Identification and characterization of two RNA silencing suppressors encoded by ophioviruses

Autores
Robles Luna, Gabriel; Reyes Martínez, Carina Andrea; Peña, Eduardo José; Ocolotobiche, Eliana Evelina; Baeza, Cecilia; Borniego, María Belén; Kormelink, Richard; García, María Laura
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Citrus psorosis virus and Mirafiori lettuce big-vein virus are two members of the genus Ophiovirus, family Ophioviridae. So far, how these viruses can interfere in the antiviral RNA silencing pathway is not known. In this study, using a local GFP silencing assay on Nicotiana benthamiana, the 24K-25K and the movement protein (MP) of both viruses were identified as RNA silencing suppressor proteins. Upon their co-expression with GFP in N. benthamiana 16c plants, the proteins also showed to suppress systemic RNA (GFP) silencing. The MPCPsV and 24KCPsV proteins bind long (114 nucleotides) but not short-interfering (21 nt) dsRNA, and upon transgenic expression, plants showed developmental abnormalities that coincided with an altered miRNA accumulation pattern. Furthermore, both proteins were able to suppress miRNA-induced silencing of a GFP-sensor construct and the co-expression of MPCPsV and 24KCPsV exhibited a stronger effect, suggesting they act at different stages of the RNAi pathway.
Instituto de Biotecnología y Biología Molecular
Materia
Biología
Ophiovirus
viral suppressor
RNA silencing
Citrus psorosis virus
Mirafiori lettuce big-vein virus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/118757
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/118757
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Identification and characterization of two RNA silencing suppressors encoded by ophiovirusesRobles Luna, GabrielReyes Martínez, Carina AndreaPeña, Eduardo JoséOcolotobiche, Eliana EvelinaBaeza, CeciliaBorniego, María BelénKormelink, RichardGarcía, María LauraBiologíaOphiovirusviral suppressorRNA silencingCitrus psorosis virusMirafiori lettuce big-vein virusCitrus psorosis virus and Mirafiori lettuce big-vein virus are two members of the genus Ophiovirus, family Ophioviridae. So far, how these viruses can interfere in the antiviral RNA silencing pathway is not known. In this study, using a local GFP silencing assay on Nicotiana benthamiana, the 24K-25K and the movement protein (MP) of both viruses were identified as RNA silencing suppressor proteins. Upon their co-expression with GFP in N. benthamiana 16c plants, the proteins also showed to suppress systemic RNA (GFP) silencing. The MPCPsV and 24KCPsV proteins bind long (114 nucleotides) but not short-interfering (21 nt) dsRNA, and upon transgenic expression, plants showed developmental abnormalities that coincided with an altered miRNA accumulation pattern. Furthermore, both proteins were able to suppress miRNA-induced silencing of a GFP-sensor construct and the co-expression of MPCPsV and 24KCPsV exhibited a stronger effect, suggesting they act at different stages of the RNAi pathway.Instituto de Biotecnología y Biología Molecular2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdf96-105http://sedici.unlp.edu.ar/handle/10915/118757enginfo:eu-repo/semantics/altIdentifier/issn/0168-1702info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virusres.2017.04.013info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-15T11:19:50Zoai:sedici.unlp.edu.ar:10915/118757Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-15 11:19:50.576SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Identification and characterization of two RNA silencing suppressors encoded by ophioviruses
title Identification and characterization of two RNA silencing suppressors encoded by ophioviruses
spellingShingle Identification and characterization of two RNA silencing suppressors encoded by ophioviruses
Robles Luna, Gabriel
Biología
Ophiovirus
viral suppressor
RNA silencing
Citrus psorosis virus
Mirafiori lettuce big-vein virus
title_short Identification and characterization of two RNA silencing suppressors encoded by ophioviruses
title_full Identification and characterization of two RNA silencing suppressors encoded by ophioviruses
title_fullStr Identification and characterization of two RNA silencing suppressors encoded by ophioviruses
title_full_unstemmed Identification and characterization of two RNA silencing suppressors encoded by ophioviruses
title_sort Identification and characterization of two RNA silencing suppressors encoded by ophioviruses
dc.creator.none.fl_str_mv Robles Luna, Gabriel
Reyes Martínez, Carina Andrea
Peña, Eduardo José
Ocolotobiche, Eliana Evelina
Baeza, Cecilia
Borniego, María Belén
Kormelink, Richard
García, María Laura
author Robles Luna, Gabriel
author_facet Robles Luna, Gabriel
Reyes Martínez, Carina Andrea
Peña, Eduardo José
Ocolotobiche, Eliana Evelina
Baeza, Cecilia
Borniego, María Belén
Kormelink, Richard
García, María Laura
author_role author
author2 Reyes Martínez, Carina Andrea
Peña, Eduardo José
Ocolotobiche, Eliana Evelina
Baeza, Cecilia
Borniego, María Belén
Kormelink, Richard
García, María Laura
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Ophiovirus
viral suppressor
RNA silencing
Citrus psorosis virus
Mirafiori lettuce big-vein virus
topic Biología
Ophiovirus
viral suppressor
RNA silencing
Citrus psorosis virus
Mirafiori lettuce big-vein virus
dc.description.none.fl_txt_mv Citrus psorosis virus and Mirafiori lettuce big-vein virus are two members of the genus Ophiovirus, family Ophioviridae. So far, how these viruses can interfere in the antiviral RNA silencing pathway is not known. In this study, using a local GFP silencing assay on Nicotiana benthamiana, the 24K-25K and the movement protein (MP) of both viruses were identified as RNA silencing suppressor proteins. Upon their co-expression with GFP in N. benthamiana 16c plants, the proteins also showed to suppress systemic RNA (GFP) silencing. The MPCPsV and 24KCPsV proteins bind long (114 nucleotides) but not short-interfering (21 nt) dsRNA, and upon transgenic expression, plants showed developmental abnormalities that coincided with an altered miRNA accumulation pattern. Furthermore, both proteins were able to suppress miRNA-induced silencing of a GFP-sensor construct and the co-expression of MPCPsV and 24KCPsV exhibited a stronger effect, suggesting they act at different stages of the RNAi pathway.
Instituto de Biotecnología y Biología Molecular
description Citrus psorosis virus and Mirafiori lettuce big-vein virus are two members of the genus Ophiovirus, family Ophioviridae. So far, how these viruses can interfere in the antiviral RNA silencing pathway is not known. In this study, using a local GFP silencing assay on Nicotiana benthamiana, the 24K-25K and the movement protein (MP) of both viruses were identified as RNA silencing suppressor proteins. Upon their co-expression with GFP in N. benthamiana 16c plants, the proteins also showed to suppress systemic RNA (GFP) silencing. The MPCPsV and 24KCPsV proteins bind long (114 nucleotides) but not short-interfering (21 nt) dsRNA, and upon transgenic expression, plants showed developmental abnormalities that coincided with an altered miRNA accumulation pattern. Furthermore, both proteins were able to suppress miRNA-induced silencing of a GFP-sensor construct and the co-expression of MPCPsV and 24KCPsV exhibited a stronger effect, suggesting they act at different stages of the RNAi pathway.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/118757
url http://sedici.unlp.edu.ar/handle/10915/118757
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/issn/0168-1702
info:eu-repo/semantics/altIdentifier/doi/10.1016/j.virusres.2017.04.013
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
96-105
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 13.22299

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