Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural...

Autores
Calderoli, Priscila A.; Behrends Kraemer, Filipe; Morrás, Héctor José María; Aguilar, O. Mario
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Calderoli, Priscila A. Instituto de Biotecnología y Biología Molecular (IBBM), Universidad Nacional de La Plata-CONICET, La Plata, Argentina.
Fil: Behrends Kraemer, Filipe. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Ingeniería Agrícola y Uso de la Tierra. Cátedra de Manejo y Conservación de Suelos. Buenos Aires, Argentina.
Fil: Behrends Kraemer, Filipe. Instituto Nacional de Tecnología Agropecuaria. Investigación de Recursos Naturales. Instituto de Suelos (INTA - CIRN). Argentina.
Fil: Morrás, Héctor José María. Instituto Nacional de Tecnología Agropecuaria. Investigación de Recursos Naturales. Instituto de Suelos (INTA - CIRN). Argentina.
Fil: Aguilar, O. Mario. Instituto de Biotecnología y Biología Molecular (IBBM), Universidad Nacional de La Plata-CONICET, La Plata, Argentina.
In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no-till management. Because crop production relies on soil-borne microorganism’s activities, such as free N2 fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.
grafs., tbls.
Fuente
Microbiology Open
Vol.6
e502
https://www.wiley.com
Materia
CYANOBACTERIA AND GEOBACTER
NIFH TRANSCRIPTS
SOIL DIAZOTROPHIC COMMUNITY
SOIL MANAGEMENT PRACTICES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
acceso abierto
Repositorio
FAUBA Digital (UBA-FAUBA)
Institución
Universidad de Buenos Aires. Facultad de Agronomía
OAI Identificador
snrd:2017calderoli

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oai_identifier_str snrd:2017calderoli
network_acronym_str FAUBA
repository_id_str 2729
network_name_str FAUBA Digital (UBA-FAUBA)
spelling Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soilCalderoli, Priscila A.Behrends Kraemer, FilipeMorrás, Héctor José MaríaAguilar, O. MarioCYANOBACTERIA AND GEOBACTERNIFH TRANSCRIPTSSOIL DIAZOTROPHIC COMMUNITYSOIL MANAGEMENT PRACTICESFil: Calderoli, Priscila A. Instituto de Biotecnología y Biología Molecular (IBBM), Universidad Nacional de La Plata-CONICET, La Plata, Argentina.Fil: Behrends Kraemer, Filipe. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Ingeniería Agrícola y Uso de la Tierra. Cátedra de Manejo y Conservación de Suelos. Buenos Aires, Argentina.Fil: Behrends Kraemer, Filipe. Instituto Nacional de Tecnología Agropecuaria. Investigación de Recursos Naturales. Instituto de Suelos (INTA - CIRN). Argentina.Fil: Morrás, Héctor José María. Instituto Nacional de Tecnología Agropecuaria. Investigación de Recursos Naturales. Instituto de Suelos (INTA - CIRN). Argentina.Fil: Aguilar, O. Mario. Instituto de Biotecnología y Biología Molecular (IBBM), Universidad Nacional de La Plata-CONICET, La Plata, Argentina.In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no-till management. Because crop production relies on soil-borne microorganism’s activities, such as free N2 fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.grafs., tbls.2017info:eu-repo/semantics/articlepublishedVersioninfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfdoi:10.1002/mbo3.502issn:2045-8827http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2017calderoliMicrobiology OpenVol.6e502https://www.wiley.comreponame:FAUBA Digital (UBA-FAUBA)instname:Universidad de Buenos Aires. Facultad de Agronomíaenginfo:eu-repo/semantics/openAccessopenAccesshttp://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section42025-09-29T13:41:45Zsnrd:2017calderoliinstacron:UBA-FAUBAInstitucionalhttp://ri.agro.uba.ar/Universidad públicaNo correspondehttp://ri.agro.uba.ar/greenstone3/oaiserver?verb=ListSetsmartino@agro.uba.ar;berasa@agro.uba.ar ArgentinaNo correspondeNo correspondeNo correspondeopendoar:27292025-09-29 13:41:46.768FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomíafalse
dc.title.none.fl_str_mv Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soil
title Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soil
spellingShingle Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soil
Calderoli, Priscila A.
CYANOBACTERIA AND GEOBACTER
NIFH TRANSCRIPTS
SOIL DIAZOTROPHIC COMMUNITY
SOIL MANAGEMENT PRACTICES
title_short Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soil
title_full Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soil
title_fullStr Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soil
title_full_unstemmed Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soil
title_sort Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soil
dc.creator.none.fl_str_mv Calderoli, Priscila A.
Behrends Kraemer, Filipe
Morrás, Héctor José María
Aguilar, O. Mario
author Calderoli, Priscila A.
author_facet Calderoli, Priscila A.
Behrends Kraemer, Filipe
Morrás, Héctor José María
Aguilar, O. Mario
author_role author
author2 Behrends Kraemer, Filipe
Morrás, Héctor José María
Aguilar, O. Mario
author2_role author
author
author
dc.subject.none.fl_str_mv CYANOBACTERIA AND GEOBACTER
NIFH TRANSCRIPTS
SOIL DIAZOTROPHIC COMMUNITY
SOIL MANAGEMENT PRACTICES
topic CYANOBACTERIA AND GEOBACTER
NIFH TRANSCRIPTS
SOIL DIAZOTROPHIC COMMUNITY
SOIL MANAGEMENT PRACTICES
dc.description.none.fl_txt_mv Fil: Calderoli, Priscila A. Instituto de Biotecnología y Biología Molecular (IBBM), Universidad Nacional de La Plata-CONICET, La Plata, Argentina.
Fil: Behrends Kraemer, Filipe. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Ingeniería Agrícola y Uso de la Tierra. Cátedra de Manejo y Conservación de Suelos. Buenos Aires, Argentina.
Fil: Behrends Kraemer, Filipe. Instituto Nacional de Tecnología Agropecuaria. Investigación de Recursos Naturales. Instituto de Suelos (INTA - CIRN). Argentina.
Fil: Morrás, Héctor José María. Instituto Nacional de Tecnología Agropecuaria. Investigación de Recursos Naturales. Instituto de Suelos (INTA - CIRN). Argentina.
Fil: Aguilar, O. Mario. Instituto de Biotecnología y Biología Molecular (IBBM), Universidad Nacional de La Plata-CONICET, La Plata, Argentina.
In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no-till management. Because crop production relies on soil-borne microorganism’s activities, such as free N2 fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.
grafs., tbls.
description Fil: Calderoli, Priscila A. Instituto de Biotecnología y Biología Molecular (IBBM), Universidad Nacional de La Plata-CONICET, La Plata, Argentina.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
publishedVersion
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv doi:10.1002/mbo3.502
issn:2045-8827
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2017calderoli
identifier_str_mv doi:10.1002/mbo3.502
issn:2045-8827
url http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2017calderoli
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
eu_rights_str_mv openAccess
rights_invalid_str_mv openAccess
http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Microbiology Open
Vol.6
e502
https://www.wiley.com
reponame:FAUBA Digital (UBA-FAUBA)
instname:Universidad de Buenos Aires. Facultad de Agronomía
reponame_str FAUBA Digital (UBA-FAUBA)
collection FAUBA Digital (UBA-FAUBA)
instname_str Universidad de Buenos Aires. Facultad de Agronomía
repository.name.fl_str_mv FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomía
repository.mail.fl_str_mv martino@agro.uba.ar;berasa@agro.uba.ar
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