Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural...
- Autores
- Calderoli, Priscila A.; Behrends Kraemer, Filipe; Morrás, Héctor José María; Aguilar, O. Mario
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Calderoli, Priscila A. Instituto de Biotecnología y Biología Molecular (IBBM), Universidad Nacional de La Plata-CONICET, La Plata, Argentina.
Fil: Behrends Kraemer, Filipe. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Ingeniería Agrícola y Uso de la Tierra. Cátedra de Manejo y Conservación de Suelos. Buenos Aires, Argentina.
Fil: Behrends Kraemer, Filipe. Instituto Nacional de Tecnología Agropecuaria. Investigación de Recursos Naturales. Instituto de Suelos (INTA - CIRN). Argentina.
Fil: Morrás, Héctor José María. Instituto Nacional de Tecnología Agropecuaria. Investigación de Recursos Naturales. Instituto de Suelos (INTA - CIRN). Argentina.
Fil: Aguilar, O. Mario. Instituto de Biotecnología y Biología Molecular (IBBM), Universidad Nacional de La Plata-CONICET, La Plata, Argentina.
In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no-till management. Because crop production relies on soil-borne microorganism’s activities, such as free N2 fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.
grafs., tbls. - Fuente
- Microbiology Open
Vol.6
e502
https://www.wiley.com - Materia
-
CYANOBACTERIA AND GEOBACTER
NIFH TRANSCRIPTS
SOIL DIAZOTROPHIC COMMUNITY
SOIL MANAGEMENT PRACTICES - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- acceso abierto
- Repositorio
- Institución
- Universidad de Buenos Aires. Facultad de Agronomía
- OAI Identificador
- snrd:2017calderoli
Ver los metadatos del registro completo
id |
FAUBA_19008d34e1cea8184c5b3c960d9b2c98 |
---|---|
oai_identifier_str |
snrd:2017calderoli |
network_acronym_str |
FAUBA |
repository_id_str |
2729 |
network_name_str |
FAUBA Digital (UBA-FAUBA) |
spelling |
Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soilCalderoli, Priscila A.Behrends Kraemer, FilipeMorrás, Héctor José MaríaAguilar, O. MarioCYANOBACTERIA AND GEOBACTERNIFH TRANSCRIPTSSOIL DIAZOTROPHIC COMMUNITYSOIL MANAGEMENT PRACTICESFil: Calderoli, Priscila A. Instituto de Biotecnología y Biología Molecular (IBBM), Universidad Nacional de La Plata-CONICET, La Plata, Argentina.Fil: Behrends Kraemer, Filipe. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Ingeniería Agrícola y Uso de la Tierra. Cátedra de Manejo y Conservación de Suelos. Buenos Aires, Argentina.Fil: Behrends Kraemer, Filipe. Instituto Nacional de Tecnología Agropecuaria. Investigación de Recursos Naturales. Instituto de Suelos (INTA - CIRN). Argentina.Fil: Morrás, Héctor José María. Instituto Nacional de Tecnología Agropecuaria. Investigación de Recursos Naturales. Instituto de Suelos (INTA - CIRN). Argentina.Fil: Aguilar, O. Mario. Instituto de Biotecnología y Biología Molecular (IBBM), Universidad Nacional de La Plata-CONICET, La Plata, Argentina.In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no-till management. Because crop production relies on soil-borne microorganism’s activities, such as free N2 fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified.grafs., tbls.2017info:eu-repo/semantics/articlepublishedVersioninfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfdoi:10.1002/mbo3.502issn:2045-8827http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2017calderoliMicrobiology OpenVol.6e502https://www.wiley.comreponame:FAUBA Digital (UBA-FAUBA)instname:Universidad de Buenos Aires. Facultad de Agronomíaenginfo:eu-repo/semantics/openAccessopenAccesshttp://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section42025-09-29T13:41:45Zsnrd:2017calderoliinstacron:UBA-FAUBAInstitucionalhttp://ri.agro.uba.ar/Universidad públicaNo correspondehttp://ri.agro.uba.ar/greenstone3/oaiserver?verb=ListSetsmartino@agro.uba.ar;berasa@agro.uba.ar ArgentinaNo correspondeNo correspondeNo correspondeopendoar:27292025-09-29 13:41:46.768FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomíafalse |
dc.title.none.fl_str_mv |
Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soil |
title |
Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soil |
spellingShingle |
Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soil Calderoli, Priscila A. CYANOBACTERIA AND GEOBACTER NIFH TRANSCRIPTS SOIL DIAZOTROPHIC COMMUNITY SOIL MANAGEMENT PRACTICES |
title_short |
Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soil |
title_full |
Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soil |
title_fullStr |
Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soil |
title_full_unstemmed |
Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soil |
title_sort |
Analysis of nifH - RNA reveals phylotypes related to Geobacter and Cyanobacteria as important functional components of the N2 - fixing community depending on depth and agricultural use of soil |
dc.creator.none.fl_str_mv |
Calderoli, Priscila A. Behrends Kraemer, Filipe Morrás, Héctor José María Aguilar, O. Mario |
author |
Calderoli, Priscila A. |
author_facet |
Calderoli, Priscila A. Behrends Kraemer, Filipe Morrás, Héctor José María Aguilar, O. Mario |
author_role |
author |
author2 |
Behrends Kraemer, Filipe Morrás, Héctor José María Aguilar, O. Mario |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
CYANOBACTERIA AND GEOBACTER NIFH TRANSCRIPTS SOIL DIAZOTROPHIC COMMUNITY SOIL MANAGEMENT PRACTICES |
topic |
CYANOBACTERIA AND GEOBACTER NIFH TRANSCRIPTS SOIL DIAZOTROPHIC COMMUNITY SOIL MANAGEMENT PRACTICES |
dc.description.none.fl_txt_mv |
Fil: Calderoli, Priscila A. Instituto de Biotecnología y Biología Molecular (IBBM), Universidad Nacional de La Plata-CONICET, La Plata, Argentina. Fil: Behrends Kraemer, Filipe. Universidad de Buenos Aires. Facultad de Agronomía. Departamento de Ingeniería Agrícola y Uso de la Tierra. Cátedra de Manejo y Conservación de Suelos. Buenos Aires, Argentina. Fil: Behrends Kraemer, Filipe. Instituto Nacional de Tecnología Agropecuaria. Investigación de Recursos Naturales. Instituto de Suelos (INTA - CIRN). Argentina. Fil: Morrás, Héctor José María. Instituto Nacional de Tecnología Agropecuaria. Investigación de Recursos Naturales. Instituto de Suelos (INTA - CIRN). Argentina. Fil: Aguilar, O. Mario. Instituto de Biotecnología y Biología Molecular (IBBM), Universidad Nacional de La Plata-CONICET, La Plata, Argentina. In this survey, a total of 80 787 reads and 28 171 unique NifH protein sequences were retrieved from soil RNA. This dataset extends our knowledge about the structure and diversity of the functional diazotrophic communities in agricultural soils from Argentinean Pampas. Operational taxonomic unit (OTU)-based analyses showed that nifH phylotypes related to Geobacter and Anaeromyxobacter (44.8%), Rhizobiales (29%), Cyanobacteria (16.7%), and Verrucomicrobiales (8%) are key microbial components of N2 fixation in soils associated with no-till management and soil depth. In addition, quantification of nifH gene copies related to Geobacter and Cyanobacteria revealed that these groups are abundant in soils under maize–soybean rotation and soybean monoculture, respectively. The correlation of physicochemical soil parameters with the diazotrophic diversity and composition showed that soil stability and organic carbon might contribute to the functional signatures of particular nifH phylotypes in fields under no-till management. Because crop production relies on soil-borne microorganism’s activities, such as free N2 fixation, the information provided by our study on the diazotrophic population dynamics, associated with the edaphic properties and land-use practices, represents a major contribution to gain insight into soil biology, in which functionally active components are identified. grafs., tbls. |
description |
Fil: Calderoli, Priscila A. Instituto de Biotecnología y Biología Molecular (IBBM), Universidad Nacional de La Plata-CONICET, La Plata, Argentina. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article publishedVersion info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
doi:10.1002/mbo3.502 issn:2045-8827 http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2017calderoli |
identifier_str_mv |
doi:10.1002/mbo3.502 issn:2045-8827 |
url |
http://ri.agro.uba.ar/greenstone3/library/collection/arti/document/2017calderoli |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
openAccess http://ri.agro.uba.ar/greenstone3/library/page/biblioteca#section4 |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Microbiology Open Vol.6 e502 https://www.wiley.com reponame:FAUBA Digital (UBA-FAUBA) instname:Universidad de Buenos Aires. Facultad de Agronomía |
reponame_str |
FAUBA Digital (UBA-FAUBA) |
collection |
FAUBA Digital (UBA-FAUBA) |
instname_str |
Universidad de Buenos Aires. Facultad de Agronomía |
repository.name.fl_str_mv |
FAUBA Digital (UBA-FAUBA) - Universidad de Buenos Aires. Facultad de Agronomía |
repository.mail.fl_str_mv |
martino@agro.uba.ar;berasa@agro.uba.ar |
_version_ |
1844618861388759040 |
score |
13.070432 |