Single yeast cell nanomotions correlate with cellular activity

Autores
Willaert, Ronnie G; Vanden Boer, Pieterjan; Malovichko, Anton; Alioscha-Perez, Mitchel; Radotić, Ksenija; Bartolić, Dragana; Kalauzi, Aleksandar; Villalba, María Inés; Sanglard, Dominique; Dietler, Giovanni; Sahli, Hichem; Kasas, Sandor
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
Materia
Biología
Yeast cells
Cellular nanomotion
Antifungal susceptibility tests
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc/4.0/
Repositorio
SEDICI (UNLP)
Institución
Universidad Nacional de La Plata
OAI Identificador
oai:sedici.unlp.edu.ar:10915/107502
Acceder
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oai_identifier_str oai:sedici.unlp.edu.ar:10915/107502
network_acronym_str SEDICI
repository_id_str 1329
network_name_str SEDICI (UNLP)
spelling Single yeast cell nanomotions correlate with cellular activityWillaert, Ronnie GVanden Boer, PieterjanMalovichko, AntonAlioscha-Perez, MitchelRadotić, KsenijaBartolić, DraganaKalauzi, AleksandarVillalba, María InésSanglard, DominiqueDietler, GiovanniSahli, HichemKasas, SandorBiologíaYeast cellsCellular nanomotionAntifungal susceptibility testsLiving single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.Centro de Investigación y Desarrollo en Fermentaciones Industriales2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionArticulohttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://sedici.unlp.edu.ar/handle/10915/107502enginfo:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7314535&blobtype=pdfinfo:eu-repo/semantics/altIdentifier/issn/2375-2548info:eu-repo/semantics/altIdentifier/pmid/32637604info:eu-repo/semantics/altIdentifier/doi/10.1126/sciadv.aba3139info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0/Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)reponame:SEDICI (UNLP)instname:Universidad Nacional de La Platainstacron:UNLP2025-10-22T17:04:47Zoai:sedici.unlp.edu.ar:10915/107502Institucionalhttp://sedici.unlp.edu.ar/Universidad públicaNo correspondehttp://sedici.unlp.edu.ar/oai/snrdalira@sedici.unlp.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:13292025-10-22 17:04:47.519SEDICI (UNLP) - Universidad Nacional de La Platafalse
dc.title.none.fl_str_mv Single yeast cell nanomotions correlate with cellular activity
title Single yeast cell nanomotions correlate with cellular activity
spellingShingle Single yeast cell nanomotions correlate with cellular activity
Willaert, Ronnie G
Biología
Yeast cells
Cellular nanomotion
Antifungal susceptibility tests
title_short Single yeast cell nanomotions correlate with cellular activity
title_full Single yeast cell nanomotions correlate with cellular activity
title_fullStr Single yeast cell nanomotions correlate with cellular activity
title_full_unstemmed Single yeast cell nanomotions correlate with cellular activity
title_sort Single yeast cell nanomotions correlate with cellular activity
dc.creator.none.fl_str_mv Willaert, Ronnie G
Vanden Boer, Pieterjan
Malovichko, Anton
Alioscha-Perez, Mitchel
Radotić, Ksenija
Bartolić, Dragana
Kalauzi, Aleksandar
Villalba, María Inés
Sanglard, Dominique
Dietler, Giovanni
Sahli, Hichem
Kasas, Sandor
author Willaert, Ronnie G
author_facet Willaert, Ronnie G
Vanden Boer, Pieterjan
Malovichko, Anton
Alioscha-Perez, Mitchel
Radotić, Ksenija
Bartolić, Dragana
Kalauzi, Aleksandar
Villalba, María Inés
Sanglard, Dominique
Dietler, Giovanni
Sahli, Hichem
Kasas, Sandor
author_role author
author2 Vanden Boer, Pieterjan
Malovichko, Anton
Alioscha-Perez, Mitchel
Radotić, Ksenija
Bartolić, Dragana
Kalauzi, Aleksandar
Villalba, María Inés
Sanglard, Dominique
Dietler, Giovanni
Sahli, Hichem
Kasas, Sandor
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biología
Yeast cells
Cellular nanomotion
Antifungal susceptibility tests
topic Biología
Yeast cells
Cellular nanomotion
Antifungal susceptibility tests
dc.description.none.fl_txt_mv Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.
Centro de Investigación y Desarrollo en Fermentaciones Industriales
description Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical optical microscopy. The distribution of the cellular displacements over a short time period is distinct from random motion. The range and shape of such nanomotion displacement distributions change substantially according to the metabolic state of the cell. The analysis of the nanomotion frequency pattern demonstrated that single living yeast cells oscillate at relatively low frequencies of around 2 hertz. The simplicity of the technique should open the way to numerous applications among which antifungal susceptibility tests seem the most straightforward.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
Articulo
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://sedici.unlp.edu.ar/handle/10915/107502
url http://sedici.unlp.edu.ar/handle/10915/107502
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC7314535&blobtype=pdf
info:eu-repo/semantics/altIdentifier/issn/2375-2548
info:eu-repo/semantics/altIdentifier/pmid/32637604
info:eu-repo/semantics/altIdentifier/doi/10.1126/sciadv.aba3139
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc/4.0/
Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:SEDICI (UNLP)
instname:Universidad Nacional de La Plata
instacron:UNLP
reponame_str SEDICI (UNLP)
collection SEDICI (UNLP)
instname_str Universidad Nacional de La Plata
instacron_str UNLP
institution UNLP
repository.name.fl_str_mv SEDICI (UNLP) - Universidad Nacional de La Plata
repository.mail.fl_str_mv alira@sedici.unlp.edu.ar
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score 12.982451

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