Epidemiology and Antifungal Susceptibilities of Yeasts Causing Vulvovaginitis in a Teaching Hospital

Autores
Gamarra, Soledad; Morano, Susana; Dudiuk, Catiana Beatriz; Mancilla, Estefanía; Nardin, María Elena; Méndez, Emilce de los Ángeles; Garcia, Guillermo Manuel
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Vulvovaginal candidiasis is one of the most common mycosis. However, the information about antifungal susceptibilities of the yeasts causing this infection is scant. We studied 121 yeasts isolated from 118 patients with vulvovaginal candidiasis. The isolates were identified by phenotypic and molecular methods, including four phenotypic methods described to differentiate Candida albicans from C. dubliniensis. Antifungal susceptibility testing was performed according to CLSI documents M27A3 and M27S4 using the drugs available as treatment option in the hospital. Diabetes, any antibacterial and amoxicillin treatment were statistically linked with vulvovaginal candidiasis, while oral contraceptives were not considered a risk factor. Previous azole-based over-the-counter antifungal treatment was statistically associated with non-C.albicans yeasts infections. The most common isolated yeast species was C. albicans (85.2 %) followed by C. glabrata (5 %), Saccharomyces cerevisiae (3.3 %), and C. dubliniensis (2.5 %). Fluconazole- and itraconazole-reduced susceptibility was observed in ten and in only one C. albicans strains, respectively. All the C. glabrata isolates showed low fluconazole MICs. Clotrimazole showed excellent potency against all but seven isolates (three C. glabrata, two S. cerevisiae, one C. albicans and one Picchia anomala). Any of the strains showed nystatin reduced susceptibility. On the other hand, terbinafine was the less potent drug. Antifungal resistance is still a rare phenomenon supporting the use of azole antifungals as empirical treatment of vulvovaginal candidiasis.
Fil: Gamarra, Soledad. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Morano, Susana. Provincia de Santa Fe. Ministerio de Salud. Hospital "Dr. José María Cullen"; Argentina
Fil: Dudiuk, Catiana Beatriz. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Mancilla, Estefanía. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Provincia de Santa Fe. Ministerio de Salud. Hospital San Carlos; Argentina
Fil: Nardin, María Elena. Provincia de Santa Fe. Ministerio de Salud. Hospital "Dr. José María Cullen"; Argentina
Fil: Méndez, Emilce de los Ángeles. Provincia de Santa Fe. Ministerio de Salud. Hospital "Dr. José María Cullen"; Argentina
Fil: Garcia, Guillermo Manuel. Provincia de Santa Fe. Ministerio de Salud. Hospital "Dr. José María Cullen"; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Materia
Yeast
Vulvovaginitis
Identification
Susceptibility
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/30933
Acceder
id CONICETDig_8b05658df4ad42bf573c51e88be9d889
oai_identifier_str oai:ri.conicet.gov.ar:11336/30933
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Epidemiology and Antifungal Susceptibilities of Yeasts Causing Vulvovaginitis in a Teaching HospitalGamarra, SoledadMorano, SusanaDudiuk, Catiana BeatrizMancilla, EstefaníaNardin, María ElenaMéndez, Emilce de los ÁngelesGarcia, Guillermo ManuelYeastVulvovaginitisIdentificationSusceptibilityhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Vulvovaginal candidiasis is one of the most common mycosis. However, the information about antifungal susceptibilities of the yeasts causing this infection is scant. We studied 121 yeasts isolated from 118 patients with vulvovaginal candidiasis. The isolates were identified by phenotypic and molecular methods, including four phenotypic methods described to differentiate Candida albicans from C. dubliniensis. Antifungal susceptibility testing was performed according to CLSI documents M27A3 and M27S4 using the drugs available as treatment option in the hospital. Diabetes, any antibacterial and amoxicillin treatment were statistically linked with vulvovaginal candidiasis, while oral contraceptives were not considered a risk factor. Previous azole-based over-the-counter antifungal treatment was statistically associated with non-C.albicans yeasts infections. The most common isolated yeast species was C. albicans (85.2 %) followed by C. glabrata (5 %), Saccharomyces cerevisiae (3.3 %), and C. dubliniensis (2.5 %). Fluconazole- and itraconazole-reduced susceptibility was observed in ten and in only one C. albicans strains, respectively. All the C. glabrata isolates showed low fluconazole MICs. Clotrimazole showed excellent potency against all but seven isolates (three C. glabrata, two S. cerevisiae, one C. albicans and one Picchia anomala). Any of the strains showed nystatin reduced susceptibility. On the other hand, terbinafine was the less potent drug. Antifungal resistance is still a rare phenomenon supporting the use of azole antifungals as empirical treatment of vulvovaginal candidiasis.Fil: Gamarra, Soledad. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; ArgentinaFil: Morano, Susana. Provincia de Santa Fe. Ministerio de Salud. Hospital "Dr. José María Cullen"; ArgentinaFil: Dudiuk, Catiana Beatriz. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaFil: Mancilla, Estefanía. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Provincia de Santa Fe. Ministerio de Salud. Hospital San Carlos; ArgentinaFil: Nardin, María Elena. Provincia de Santa Fe. Ministerio de Salud. Hospital "Dr. José María Cullen"; ArgentinaFil: Méndez, Emilce de los Ángeles. Provincia de Santa Fe. Ministerio de Salud. Hospital "Dr. José María Cullen"; ArgentinaFil: Garcia, Guillermo Manuel. Provincia de Santa Fe. Ministerio de Salud. Hospital "Dr. José María Cullen"; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; ArgentinaSpringer2014-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/30933Garcia, Guillermo Manuel; Méndez, Emilce de los Ángeles; Nardin, María Elena; Mancilla, Estefanía; Dudiuk, Catiana Beatriz; Morano, Susana; et al.; Epidemiology and Antifungal Susceptibilities of Yeasts Causing Vulvovaginitis in a Teaching Hospital; Springer; Mycopathologia; 178; 3-4; 7-2014; 251-2580301-486X1573-0832CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s11046-014-9780-2info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11046-014-9780-2info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-22T11:48:19Zoai:ri.conicet.gov.ar:11336/30933instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-22 11:48:19.745CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Epidemiology and Antifungal Susceptibilities of Yeasts Causing Vulvovaginitis in a Teaching Hospital
title Epidemiology and Antifungal Susceptibilities of Yeasts Causing Vulvovaginitis in a Teaching Hospital
spellingShingle Epidemiology and Antifungal Susceptibilities of Yeasts Causing Vulvovaginitis in a Teaching Hospital
Gamarra, Soledad
Yeast
Vulvovaginitis
Identification
Susceptibility
title_short Epidemiology and Antifungal Susceptibilities of Yeasts Causing Vulvovaginitis in a Teaching Hospital
title_full Epidemiology and Antifungal Susceptibilities of Yeasts Causing Vulvovaginitis in a Teaching Hospital
title_fullStr Epidemiology and Antifungal Susceptibilities of Yeasts Causing Vulvovaginitis in a Teaching Hospital
title_full_unstemmed Epidemiology and Antifungal Susceptibilities of Yeasts Causing Vulvovaginitis in a Teaching Hospital
title_sort Epidemiology and Antifungal Susceptibilities of Yeasts Causing Vulvovaginitis in a Teaching Hospital
dc.creator.none.fl_str_mv Gamarra, Soledad
Morano, Susana
Dudiuk, Catiana Beatriz
Mancilla, Estefanía
Nardin, María Elena
Méndez, Emilce de los Ángeles
Garcia, Guillermo Manuel
author Gamarra, Soledad
author_facet Gamarra, Soledad
Morano, Susana
Dudiuk, Catiana Beatriz
Mancilla, Estefanía
Nardin, María Elena
Méndez, Emilce de los Ángeles
Garcia, Guillermo Manuel
author_role author
author2 Morano, Susana
Dudiuk, Catiana Beatriz
Mancilla, Estefanía
Nardin, María Elena
Méndez, Emilce de los Ángeles
Garcia, Guillermo Manuel
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Yeast
Vulvovaginitis
Identification
Susceptibility
topic Yeast
Vulvovaginitis
Identification
Susceptibility
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Vulvovaginal candidiasis is one of the most common mycosis. However, the information about antifungal susceptibilities of the yeasts causing this infection is scant. We studied 121 yeasts isolated from 118 patients with vulvovaginal candidiasis. The isolates were identified by phenotypic and molecular methods, including four phenotypic methods described to differentiate Candida albicans from C. dubliniensis. Antifungal susceptibility testing was performed according to CLSI documents M27A3 and M27S4 using the drugs available as treatment option in the hospital. Diabetes, any antibacterial and amoxicillin treatment were statistically linked with vulvovaginal candidiasis, while oral contraceptives were not considered a risk factor. Previous azole-based over-the-counter antifungal treatment was statistically associated with non-C.albicans yeasts infections. The most common isolated yeast species was C. albicans (85.2 %) followed by C. glabrata (5 %), Saccharomyces cerevisiae (3.3 %), and C. dubliniensis (2.5 %). Fluconazole- and itraconazole-reduced susceptibility was observed in ten and in only one C. albicans strains, respectively. All the C. glabrata isolates showed low fluconazole MICs. Clotrimazole showed excellent potency against all but seven isolates (three C. glabrata, two S. cerevisiae, one C. albicans and one Picchia anomala). Any of the strains showed nystatin reduced susceptibility. On the other hand, terbinafine was the less potent drug. Antifungal resistance is still a rare phenomenon supporting the use of azole antifungals as empirical treatment of vulvovaginal candidiasis.
Fil: Gamarra, Soledad. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina
Fil: Morano, Susana. Provincia de Santa Fe. Ministerio de Salud. Hospital "Dr. José María Cullen"; Argentina
Fil: Dudiuk, Catiana Beatriz. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
Fil: Mancilla, Estefanía. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Provincia de Santa Fe. Ministerio de Salud. Hospital San Carlos; Argentina
Fil: Nardin, María Elena. Provincia de Santa Fe. Ministerio de Salud. Hospital "Dr. José María Cullen"; Argentina
Fil: Méndez, Emilce de los Ángeles. Provincia de Santa Fe. Ministerio de Salud. Hospital "Dr. José María Cullen"; Argentina
Fil: Garcia, Guillermo Manuel. Provincia de Santa Fe. Ministerio de Salud. Hospital "Dr. José María Cullen"; Argentina. Universidad Nacional del Litoral. Facultad de Bioquímica y Ciencias Biológicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe; Argentina
description Vulvovaginal candidiasis is one of the most common mycosis. However, the information about antifungal susceptibilities of the yeasts causing this infection is scant. We studied 121 yeasts isolated from 118 patients with vulvovaginal candidiasis. The isolates were identified by phenotypic and molecular methods, including four phenotypic methods described to differentiate Candida albicans from C. dubliniensis. Antifungal susceptibility testing was performed according to CLSI documents M27A3 and M27S4 using the drugs available as treatment option in the hospital. Diabetes, any antibacterial and amoxicillin treatment were statistically linked with vulvovaginal candidiasis, while oral contraceptives were not considered a risk factor. Previous azole-based over-the-counter antifungal treatment was statistically associated with non-C.albicans yeasts infections. The most common isolated yeast species was C. albicans (85.2 %) followed by C. glabrata (5 %), Saccharomyces cerevisiae (3.3 %), and C. dubliniensis (2.5 %). Fluconazole- and itraconazole-reduced susceptibility was observed in ten and in only one C. albicans strains, respectively. All the C. glabrata isolates showed low fluconazole MICs. Clotrimazole showed excellent potency against all but seven isolates (three C. glabrata, two S. cerevisiae, one C. albicans and one Picchia anomala). Any of the strains showed nystatin reduced susceptibility. On the other hand, terbinafine was the less potent drug. Antifungal resistance is still a rare phenomenon supporting the use of azole antifungals as empirical treatment of vulvovaginal candidiasis.
publishDate 2014
dc.date.none.fl_str_mv 2014-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/30933
Garcia, Guillermo Manuel; Méndez, Emilce de los Ángeles; Nardin, María Elena; Mancilla, Estefanía; Dudiuk, Catiana Beatriz; Morano, Susana; et al.; Epidemiology and Antifungal Susceptibilities of Yeasts Causing Vulvovaginitis in a Teaching Hospital; Springer; Mycopathologia; 178; 3-4; 7-2014; 251-258
0301-486X
1573-0832
CONICET Digital
CONICET
url http://hdl.handle.net/11336/30933
identifier_str_mv Garcia, Guillermo Manuel; Méndez, Emilce de los Ángeles; Nardin, María Elena; Mancilla, Estefanía; Dudiuk, Catiana Beatriz; Morano, Susana; et al.; Epidemiology and Antifungal Susceptibilities of Yeasts Causing Vulvovaginitis in a Teaching Hospital; Springer; Mycopathologia; 178; 3-4; 7-2014; 251-258
0301-486X
1573-0832
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s11046-014-9780-2
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007%2Fs11046-014-9780-2
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 12.982451

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