Musa spp. cultivars as a neutralising source against some toxic activities of Bothrops and Crotalus genus snake venoms

• Autores: Matos da Silva, Márcia; Pereira Alexandre, Gerso; Magalhães, Marta Regina; Torres, Ana Maria; Kato, Lucilia; Costa da Silva, Victória; Teixeira de Saboia Morais, Simone Maria; Garcia Rodriguez, Armando; Pacheco Fill, Taícia; Pereira, Alana Kelyene; Roque, Jussara; Souza Simão, Jorge Luiz; Pasqualotto Severino, Vanessa Gisele
• Año de publicación: 2023
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Toxicon, 2023, vol. 228, p. 1-12.
• Materia: Musa spp.; Banana; Sap; Antivenom; Bothrops; Crotalus
• Institución: Universidad Nacional del Nordeste

Fil: Matos da Silva, Márcia. Universidade Federal de Goiás. Instituto de Química; Brasil.
Fil: Pereira Alexandre, Gerso. Universidade Federal de Goiás. Instituto de Química; Brasil.
Fil: Magalhães, Marta Regina. Pontificia Universidad Católica de Goiás. Escuela de Ciencias Médicas y de la Vida. Centro de Estudios e Investigaciones Biológicas; Brasil.
Fil: Torres, Ana Maria. Universidad Nacional del Nordeste. Laboratorio de Productos Naturales Prof. Armando Ricciardi; Argentina.
Fil: Torres, Ana María. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina.
Fil: Kato, Lucilia. Universidade Federal de Goiás. Instituto de Química; Brasil.
Fil: Costa da Silva, Victória. Universidade Federal de Goiás. Instituto de Ciencias Biológicas; Brasil.
Fil: Teixeira de Saboia Morais, Simone Maria. Universidade Estadual de Campinas. Instituto de Ciencias Biológicas; Brasil.
Fil: Garcia Rodriguez, Armando. Universidade Federal de Goiás. Instituto de Ciencias Biológicas; Brasil.
Fil: Pacheco Fil, Taícia. Universidade Estadual de Campinas. Instituto de Química; Brasil.
Fil: Pereira, Alana Kelyene. Universidade Estadual de Campinas. Instituto de Química; Brasil.
Fil: Roque, Jussara. Universidade Federal de Goiás. Instituto de Química; Brasil.
Fil: Souza Simão, Jorge Luiz. Universidade Federal de Goiás. Instituto de Química; Brasil.
Fil: Pasqualotto Severino, Vanessa Gisele. Universidade Federal de Goiás. Instituto de Química; Brasil.
Accidents involving snakes from Bothrops spp. and Crotalus spp. constitute the most important cause of envenomation in Brazil and Argentina. Musa spp. (banana) have been reported to be used in popular medicine against snakebite by the members of the Canudos Settlement, located in Goiás. In this way, the aim of this work was to evaluate the antivenom effect of the Ouro (AA), Prata (AAB), Prata-anã (AAB) and Figo (ABB) cultivars against in vitro (phospholipase, coagulation and proteolytic) and in vivo (lethality and toxicity) activities caused by the venoms and toxicity (Artemia salina nauplii and Danio rerio embryos) of Musa spp. as well as the annotation of chemical compounds possibly related to these activities. From the in vitro antiophidic tests with the sap, we observed 100% inhibition of the phospholipase and coagulant activities with the cultivars Prata-anã and Figo against the venoms of B. alternatus and C. d. collineatus, B. diporus and B. pauloensis, respectively, and neutralisation of the lethality against the B. diporus venom. It was observed that the cultivars of Musa spp. did not show toxicity against Artemia salina nauplii and Danio rerio embryos. The sap analysis via HPLC-MS/MS allowed the annotation of the 13 compounds: abscisic acid, shikimic acid, citric acid, quinic acid, afzelechin, Glp-hexose, glucose, sucrose, isorhamnetin-3-O-galactoside-6-raminoside, kaempferol-3-glucoside-3-raminoside, myricetin-3-O-rutinoside, procyanidin B1 and rutin. Therefore, it can be seen that Musa spp. is a potential therapeutic agent that can act to neutralise the effects caused by snakebites.