Antivenom against Crotalus durissus terrificus venom: Immunochemical reactivity and experimental neutralizing capacity

Autores
Baudou, Federico Gastón; Litwin, Silvana; Lanari, Laura Cecilia; Laskowicz, Rodrigo Daniel; Damin, Carlos Fabian; Chippaux, Jean-Philippe; de Roodt, Adolfo Rafael
Año de publicación
2017
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The venom of Crotalus durissus terrificus produces a neurotoxic and myotoxic syndrome that can lead to the death. Specific antivenom is the only treatment to neutralize the toxicity of the venom and the precocity in applying the antivenom is crucial for the efficiency of the treatment. We studied the variation of the immunochemical reactivity and neutralizing capacity of the specific antivenom on this venom in pre-incubation and rescue experiments, at different times. ELISA titers increased with longer venom-antivenom incubation times (p < 0.05) nevertheless incubation times had no effect on the neutralizing capacity of the antivenom. The antivenom dose necessary to rescue mice injected with 1.5 MMD (minimal mortal dose) 30 min after venom inoculation was over ten folds the dose of antivenom theoretically required to neutralize the same dose of venom according values obtained from pre-incubation experiments. Results showed that the in vitro immunochemical reactivity is not directly related to the neutralizing capacity. These observations underline the need for a rapid antivenom administration. Although preincubation experiments in mice are a powerful tool for the validation of the potency of the antivenoms in the productive process, it is clear that the nominal neutralization of the antivenoms must not be considered as a “stoichiometric” value regarding the venom to be neutralized in case of natural envenomation and emphasize the need of realization of clinical trials in order to evaluate the adequate doses of antivenom to be therapeutically used.
Fil: Baudou, Federico Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Litwin, Silvana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Lanari, Laura Cecilia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Laskowicz, Rodrigo Daniel. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Damin, Carlos Fabian. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Chippaux, Jean-Philippe. Universite de Paris I Pantheon - Sorbonne; Francia
Fil: de Roodt, Adolfo Rafael. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Materia
Antivenom
Crotalus Durissus Terrificus
Dosage
Incubation
Neutralization
Rescue
Treatment
Venom
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/72109
Acceder
id CONICETDig_f6010c28e2654487d040893510418ffd
oai_identifier_str oai:ri.conicet.gov.ar:11336/72109
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Antivenom against Crotalus durissus terrificus venom: Immunochemical reactivity and experimental neutralizing capacityBaudou, Federico GastónLitwin, SilvanaLanari, Laura CeciliaLaskowicz, Rodrigo DanielDamin, Carlos FabianChippaux, Jean-Philippede Roodt, Adolfo RafaelAntivenomCrotalus Durissus TerrificusDosageIncubationNeutralizationRescueTreatmentVenomhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1The venom of Crotalus durissus terrificus produces a neurotoxic and myotoxic syndrome that can lead to the death. Specific antivenom is the only treatment to neutralize the toxicity of the venom and the precocity in applying the antivenom is crucial for the efficiency of the treatment. We studied the variation of the immunochemical reactivity and neutralizing capacity of the specific antivenom on this venom in pre-incubation and rescue experiments, at different times. ELISA titers increased with longer venom-antivenom incubation times (p < 0.05) nevertheless incubation times had no effect on the neutralizing capacity of the antivenom. The antivenom dose necessary to rescue mice injected with 1.5 MMD (minimal mortal dose) 30 min after venom inoculation was over ten folds the dose of antivenom theoretically required to neutralize the same dose of venom according values obtained from pre-incubation experiments. Results showed that the in vitro immunochemical reactivity is not directly related to the neutralizing capacity. These observations underline the need for a rapid antivenom administration. Although preincubation experiments in mice are a powerful tool for the validation of the potency of the antivenoms in the productive process, it is clear that the nominal neutralization of the antivenoms must not be considered as a “stoichiometric” value regarding the venom to be neutralized in case of natural envenomation and emphasize the need of realization of clinical trials in order to evaluate the adequate doses of antivenom to be therapeutically used.Fil: Baudou, Federico Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Litwin, Silvana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Lanari, Laura Cecilia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Laskowicz, Rodrigo Daniel. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaFil: Damin, Carlos Fabian. Universidad de Buenos Aires. Facultad de Medicina; ArgentinaFil: Chippaux, Jean-Philippe. Universite de Paris I Pantheon - Sorbonne; FranciaFil: de Roodt, Adolfo Rafael. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; ArgentinaPergamon-Elsevier Science Ltd2017-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/72109Baudou, Federico Gastón; Litwin, Silvana; Lanari, Laura Cecilia; Laskowicz, Rodrigo Daniel; Damin, Carlos Fabian; et al.; Antivenom against Crotalus durissus terrificus venom: Immunochemical reactivity and experimental neutralizing capacity; Pergamon-Elsevier Science Ltd; Toxicon; 140; 12-2017; 11-170041-0101CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxicon.2017.10.009info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0041010117303069info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:44:48Zoai:ri.conicet.gov.ar:11336/72109instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:44:49.007CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Antivenom against Crotalus durissus terrificus venom: Immunochemical reactivity and experimental neutralizing capacity
title Antivenom against Crotalus durissus terrificus venom: Immunochemical reactivity and experimental neutralizing capacity
spellingShingle Antivenom against Crotalus durissus terrificus venom: Immunochemical reactivity and experimental neutralizing capacity
Baudou, Federico Gastón
Antivenom
Crotalus Durissus Terrificus
Dosage
Incubation
Neutralization
Rescue
Treatment
Venom
title_short Antivenom against Crotalus durissus terrificus venom: Immunochemical reactivity and experimental neutralizing capacity
title_full Antivenom against Crotalus durissus terrificus venom: Immunochemical reactivity and experimental neutralizing capacity
title_fullStr Antivenom against Crotalus durissus terrificus venom: Immunochemical reactivity and experimental neutralizing capacity
title_full_unstemmed Antivenom against Crotalus durissus terrificus venom: Immunochemical reactivity and experimental neutralizing capacity
title_sort Antivenom against Crotalus durissus terrificus venom: Immunochemical reactivity and experimental neutralizing capacity
dc.creator.none.fl_str_mv Baudou, Federico Gastón
Litwin, Silvana
Lanari, Laura Cecilia
Laskowicz, Rodrigo Daniel
Damin, Carlos Fabian
Chippaux, Jean-Philippe
de Roodt, Adolfo Rafael
author Baudou, Federico Gastón
author_facet Baudou, Federico Gastón
Litwin, Silvana
Lanari, Laura Cecilia
Laskowicz, Rodrigo Daniel
Damin, Carlos Fabian
Chippaux, Jean-Philippe
de Roodt, Adolfo Rafael
author_role author
author2 Litwin, Silvana
Lanari, Laura Cecilia
Laskowicz, Rodrigo Daniel
Damin, Carlos Fabian
Chippaux, Jean-Philippe
de Roodt, Adolfo Rafael
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Antivenom
Crotalus Durissus Terrificus
Dosage
Incubation
Neutralization
Rescue
Treatment
Venom
topic Antivenom
Crotalus Durissus Terrificus
Dosage
Incubation
Neutralization
Rescue
Treatment
Venom
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv The venom of Crotalus durissus terrificus produces a neurotoxic and myotoxic syndrome that can lead to the death. Specific antivenom is the only treatment to neutralize the toxicity of the venom and the precocity in applying the antivenom is crucial for the efficiency of the treatment. We studied the variation of the immunochemical reactivity and neutralizing capacity of the specific antivenom on this venom in pre-incubation and rescue experiments, at different times. ELISA titers increased with longer venom-antivenom incubation times (p < 0.05) nevertheless incubation times had no effect on the neutralizing capacity of the antivenom. The antivenom dose necessary to rescue mice injected with 1.5 MMD (minimal mortal dose) 30 min after venom inoculation was over ten folds the dose of antivenom theoretically required to neutralize the same dose of venom according values obtained from pre-incubation experiments. Results showed that the in vitro immunochemical reactivity is not directly related to the neutralizing capacity. These observations underline the need for a rapid antivenom administration. Although preincubation experiments in mice are a powerful tool for the validation of the potency of the antivenoms in the productive process, it is clear that the nominal neutralization of the antivenoms must not be considered as a “stoichiometric” value regarding the venom to be neutralized in case of natural envenomation and emphasize the need of realization of clinical trials in order to evaluate the adequate doses of antivenom to be therapeutically used.
Fil: Baudou, Federico Gastón. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Litwin, Silvana. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Lanari, Laura Cecilia. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Laskowicz, Rodrigo Daniel. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
Fil: Damin, Carlos Fabian. Universidad de Buenos Aires. Facultad de Medicina; Argentina
Fil: Chippaux, Jean-Philippe. Universite de Paris I Pantheon - Sorbonne; Francia
Fil: de Roodt, Adolfo Rafael. Universidad de Buenos Aires. Facultad de Medicina; Argentina. Dirección Nacional de Instituto de Investigación. Administración Nacional de Laboratorio e Instituto de Salud “Dr. C. G. Malbrán”; Argentina
description The venom of Crotalus durissus terrificus produces a neurotoxic and myotoxic syndrome that can lead to the death. Specific antivenom is the only treatment to neutralize the toxicity of the venom and the precocity in applying the antivenom is crucial for the efficiency of the treatment. We studied the variation of the immunochemical reactivity and neutralizing capacity of the specific antivenom on this venom in pre-incubation and rescue experiments, at different times. ELISA titers increased with longer venom-antivenom incubation times (p < 0.05) nevertheless incubation times had no effect on the neutralizing capacity of the antivenom. The antivenom dose necessary to rescue mice injected with 1.5 MMD (minimal mortal dose) 30 min after venom inoculation was over ten folds the dose of antivenom theoretically required to neutralize the same dose of venom according values obtained from pre-incubation experiments. Results showed that the in vitro immunochemical reactivity is not directly related to the neutralizing capacity. These observations underline the need for a rapid antivenom administration. Although preincubation experiments in mice are a powerful tool for the validation of the potency of the antivenoms in the productive process, it is clear that the nominal neutralization of the antivenoms must not be considered as a “stoichiometric” value regarding the venom to be neutralized in case of natural envenomation and emphasize the need of realization of clinical trials in order to evaluate the adequate doses of antivenom to be therapeutically used.
publishDate 2017
dc.date.none.fl_str_mv 2017-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/72109
Baudou, Federico Gastón; Litwin, Silvana; Lanari, Laura Cecilia; Laskowicz, Rodrigo Daniel; Damin, Carlos Fabian; et al.; Antivenom against Crotalus durissus terrificus venom: Immunochemical reactivity and experimental neutralizing capacity; Pergamon-Elsevier Science Ltd; Toxicon; 140; 12-2017; 11-17
0041-0101
CONICET Digital
CONICET
url http://hdl.handle.net/11336/72109
identifier_str_mv Baudou, Federico Gastón; Litwin, Silvana; Lanari, Laura Cecilia; Laskowicz, Rodrigo Daniel; Damin, Carlos Fabian; et al.; Antivenom against Crotalus durissus terrificus venom: Immunochemical reactivity and experimental neutralizing capacity; Pergamon-Elsevier Science Ltd; Toxicon; 140; 12-2017; 11-17
0041-0101
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.toxicon.2017.10.009
info:eu-repo/semantics/altIdentifier/url/https://linkinghub.elsevier.com/retrieve/pii/S0041010117303069
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
publisher.none.fl_str_mv Pergamon-Elsevier Science Ltd
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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