IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina

Autores
Rodríguez, Juan Pablo; de Marzi, Mauricio Cesar; Maruñak, Silvana; Teibler, Gladys Pamela; Acosta, Ofelia Cristina; Malchiodi, Emilio Luis; Leiva, Laura Cristina Ana
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50: 2.1 mg IgG anti-crotalic PLA2/100 μg of venom) were also able to neutralize venom from other snakes in the following proportion: 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In addition, in a myotoxic activity neutralization test, measured by plasma activity of creatine kinase, 35% of B. alternatus venom and 26% of B. diporus venom were neutralized, while no neutralization was detected with B. jararacussu venom. This study presents original data concerning cross-reactions between bothropic venoms from Argentina and IgG anti-crotalic PLA2. Our results suggest that anti-crotalic PLA2 antibodies should not be used to neutralize PLA2 activity of B. alternatus, B. diporus and especially B. jararacussu venoms; nor to enrich commercial antivenoms against these Bothrops species.
Fil: Rodríguez, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica; Argentina
Fil: de Marzi, Mauricio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Maruñak, Silvana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Teibler, Gladys Pamela. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Acosta, Ofelia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Leiva, Laura Cristina Ana. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina
Materia
BOTHROPS
CROSS-REACTION
CROTALUS
NEUTRALIZATION
PLA2
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/61274

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spelling IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from ArgentinaRodríguez, Juan Pablode Marzi, Mauricio CesarMaruñak, SilvanaTeibler, Gladys PamelaAcosta, Ofelia CristinaMalchiodi, Emilio LuisLeiva, Laura Cristina AnaBOTHROPSCROSS-REACTIONCROTALUSNEUTRALIZATIONPLA2https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50: 2.1 mg IgG anti-crotalic PLA2/100 μg of venom) were also able to neutralize venom from other snakes in the following proportion: 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In addition, in a myotoxic activity neutralization test, measured by plasma activity of creatine kinase, 35% of B. alternatus venom and 26% of B. diporus venom were neutralized, while no neutralization was detected with B. jararacussu venom. This study presents original data concerning cross-reactions between bothropic venoms from Argentina and IgG anti-crotalic PLA2. Our results suggest that anti-crotalic PLA2 antibodies should not be used to neutralize PLA2 activity of B. alternatus, B. diporus and especially B. jararacussu venoms; nor to enrich commercial antivenoms against these Bothrops species.Fil: Rodríguez, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica; ArgentinaFil: de Marzi, Mauricio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Maruñak, Silvana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Teibler, Gladys Pamela. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Acosta, Ofelia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Leiva, Laura Cristina Ana. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; ArgentinaCevap-unesp2009-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61274Rodríguez, Juan Pablo; de Marzi, Mauricio Cesar; Maruñak, Silvana; Teibler, Gladys Pamela; Acosta, Ofelia Cristina; et al.; IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina; Cevap-unesp; Journal Of Venomous Animals And Toxins Including Tropical Diseases; 15; 3; 3-2009; 460-4781678-9199CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/S1678-91992009000300008info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/vqjcf4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:57Zoai:ri.conicet.gov.ar:11336/61274instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:57.664CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina
title IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina
spellingShingle IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina
Rodríguez, Juan Pablo
BOTHROPS
CROSS-REACTION
CROTALUS
NEUTRALIZATION
PLA2
title_short IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina
title_full IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina
title_fullStr IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina
title_full_unstemmed IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina
title_sort IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina
dc.creator.none.fl_str_mv Rodríguez, Juan Pablo
de Marzi, Mauricio Cesar
Maruñak, Silvana
Teibler, Gladys Pamela
Acosta, Ofelia Cristina
Malchiodi, Emilio Luis
Leiva, Laura Cristina Ana
author Rodríguez, Juan Pablo
author_facet Rodríguez, Juan Pablo
de Marzi, Mauricio Cesar
Maruñak, Silvana
Teibler, Gladys Pamela
Acosta, Ofelia Cristina
Malchiodi, Emilio Luis
Leiva, Laura Cristina Ana
author_role author
author2 de Marzi, Mauricio Cesar
Maruñak, Silvana
Teibler, Gladys Pamela
Acosta, Ofelia Cristina
Malchiodi, Emilio Luis
Leiva, Laura Cristina Ana
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv BOTHROPS
CROSS-REACTION
CROTALUS
NEUTRALIZATION
PLA2
topic BOTHROPS
CROSS-REACTION
CROTALUS
NEUTRALIZATION
PLA2
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50: 2.1 mg IgG anti-crotalic PLA2/100 μg of venom) were also able to neutralize venom from other snakes in the following proportion: 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In addition, in a myotoxic activity neutralization test, measured by plasma activity of creatine kinase, 35% of B. alternatus venom and 26% of B. diporus venom were neutralized, while no neutralization was detected with B. jararacussu venom. This study presents original data concerning cross-reactions between bothropic venoms from Argentina and IgG anti-crotalic PLA2. Our results suggest that anti-crotalic PLA2 antibodies should not be used to neutralize PLA2 activity of B. alternatus, B. diporus and especially B. jararacussu venoms; nor to enrich commercial antivenoms against these Bothrops species.
Fil: Rodríguez, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica; Argentina
Fil: de Marzi, Mauricio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Maruñak, Silvana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Teibler, Gladys Pamela. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Acosta, Ofelia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Leiva, Laura Cristina Ana. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina
description We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50: 2.1 mg IgG anti-crotalic PLA2/100 μg of venom) were also able to neutralize venom from other snakes in the following proportion: 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In addition, in a myotoxic activity neutralization test, measured by plasma activity of creatine kinase, 35% of B. alternatus venom and 26% of B. diporus venom were neutralized, while no neutralization was detected with B. jararacussu venom. This study presents original data concerning cross-reactions between bothropic venoms from Argentina and IgG anti-crotalic PLA2. Our results suggest that anti-crotalic PLA2 antibodies should not be used to neutralize PLA2 activity of B. alternatus, B. diporus and especially B. jararacussu venoms; nor to enrich commercial antivenoms against these Bothrops species.
publishDate 2009
dc.date.none.fl_str_mv 2009-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
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info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/61274
Rodríguez, Juan Pablo; de Marzi, Mauricio Cesar; Maruñak, Silvana; Teibler, Gladys Pamela; Acosta, Ofelia Cristina; et al.; IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina; Cevap-unesp; Journal Of Venomous Animals And Toxins Including Tropical Diseases; 15; 3; 3-2009; 460-478
1678-9199
CONICET Digital
CONICET
url http://hdl.handle.net/11336/61274
identifier_str_mv Rodríguez, Juan Pablo; de Marzi, Mauricio Cesar; Maruñak, Silvana; Teibler, Gladys Pamela; Acosta, Ofelia Cristina; et al.; IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina; Cevap-unesp; Journal Of Venomous Animals And Toxins Including Tropical Diseases; 15; 3; 3-2009; 460-478
1678-9199
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1590/S1678-91992009000300008
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/vqjcf4
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Cevap-unesp
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