IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina
- Autores
- Rodríguez, Juan Pablo; de Marzi, Mauricio Cesar; Maruñak, Silvana; Teibler, Gladys Pamela; Acosta, Ofelia Cristina; Malchiodi, Emilio Luis; Leiva, Laura Cristina Ana
- Año de publicación
- 2009
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50: 2.1 mg IgG anti-crotalic PLA2/100 μg of venom) were also able to neutralize venom from other snakes in the following proportion: 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In addition, in a myotoxic activity neutralization test, measured by plasma activity of creatine kinase, 35% of B. alternatus venom and 26% of B. diporus venom were neutralized, while no neutralization was detected with B. jararacussu venom. This study presents original data concerning cross-reactions between bothropic venoms from Argentina and IgG anti-crotalic PLA2. Our results suggest that anti-crotalic PLA2 antibodies should not be used to neutralize PLA2 activity of B. alternatus, B. diporus and especially B. jararacussu venoms; nor to enrich commercial antivenoms against these Bothrops species.
Fil: Rodríguez, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica; Argentina
Fil: de Marzi, Mauricio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Maruñak, Silvana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Teibler, Gladys Pamela. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Acosta, Ofelia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina
Fil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Leiva, Laura Cristina Ana. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina - Materia
-
BOTHROPS
CROSS-REACTION
CROTALUS
NEUTRALIZATION
PLA2 - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc/2.5/ar/
- Repositorio
- Institución
- Consejo Nacional de Investigaciones Científicas y Técnicas
- OAI Identificador
- oai:ri.conicet.gov.ar:11336/61274
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IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from ArgentinaRodríguez, Juan Pablode Marzi, Mauricio CesarMaruñak, SilvanaTeibler, Gladys PamelaAcosta, Ofelia CristinaMalchiodi, Emilio LuisLeiva, Laura Cristina AnaBOTHROPSCROSS-REACTIONCROTALUSNEUTRALIZATIONPLA2https://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50: 2.1 mg IgG anti-crotalic PLA2/100 μg of venom) were also able to neutralize venom from other snakes in the following proportion: 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In addition, in a myotoxic activity neutralization test, measured by plasma activity of creatine kinase, 35% of B. alternatus venom and 26% of B. diporus venom were neutralized, while no neutralization was detected with B. jararacussu venom. This study presents original data concerning cross-reactions between bothropic venoms from Argentina and IgG anti-crotalic PLA2. Our results suggest that anti-crotalic PLA2 antibodies should not be used to neutralize PLA2 activity of B. alternatus, B. diporus and especially B. jararacussu venoms; nor to enrich commercial antivenoms against these Bothrops species.Fil: Rodríguez, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica; ArgentinaFil: de Marzi, Mauricio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Maruñak, Silvana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Teibler, Gladys Pamela. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Acosta, Ofelia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; ArgentinaFil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Leiva, Laura Cristina Ana. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; ArgentinaCevap-unesp2009-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/61274Rodríguez, Juan Pablo; de Marzi, Mauricio Cesar; Maruñak, Silvana; Teibler, Gladys Pamela; Acosta, Ofelia Cristina; et al.; IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina; Cevap-unesp; Journal Of Venomous Animals And Toxins Including Tropical Diseases; 15; 3; 3-2009; 460-4781678-9199CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/S1678-91992009000300008info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/vqjcf4info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:18:57Zoai:ri.conicet.gov.ar:11336/61274instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:18:57.664CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse |
dc.title.none.fl_str_mv |
IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina |
title |
IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina |
spellingShingle |
IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina Rodríguez, Juan Pablo BOTHROPS CROSS-REACTION CROTALUS NEUTRALIZATION PLA2 |
title_short |
IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina |
title_full |
IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina |
title_fullStr |
IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina |
title_full_unstemmed |
IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina |
title_sort |
IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina |
dc.creator.none.fl_str_mv |
Rodríguez, Juan Pablo de Marzi, Mauricio Cesar Maruñak, Silvana Teibler, Gladys Pamela Acosta, Ofelia Cristina Malchiodi, Emilio Luis Leiva, Laura Cristina Ana |
author |
Rodríguez, Juan Pablo |
author_facet |
Rodríguez, Juan Pablo de Marzi, Mauricio Cesar Maruñak, Silvana Teibler, Gladys Pamela Acosta, Ofelia Cristina Malchiodi, Emilio Luis Leiva, Laura Cristina Ana |
author_role |
author |
author2 |
de Marzi, Mauricio Cesar Maruñak, Silvana Teibler, Gladys Pamela Acosta, Ofelia Cristina Malchiodi, Emilio Luis Leiva, Laura Cristina Ana |
author2_role |
author author author author author author |
dc.subject.none.fl_str_mv |
BOTHROPS CROSS-REACTION CROTALUS NEUTRALIZATION PLA2 |
topic |
BOTHROPS CROSS-REACTION CROTALUS NEUTRALIZATION PLA2 |
purl_subject.fl_str_mv |
https://purl.org/becyt/ford/4.3 https://purl.org/becyt/ford/4 |
dc.description.none.fl_txt_mv |
We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50: 2.1 mg IgG anti-crotalic PLA2/100 μg of venom) were also able to neutralize venom from other snakes in the following proportion: 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In addition, in a myotoxic activity neutralization test, measured by plasma activity of creatine kinase, 35% of B. alternatus venom and 26% of B. diporus venom were neutralized, while no neutralization was detected with B. jararacussu venom. This study presents original data concerning cross-reactions between bothropic venoms from Argentina and IgG anti-crotalic PLA2. Our results suggest that anti-crotalic PLA2 antibodies should not be used to neutralize PLA2 activity of B. alternatus, B. diporus and especially B. jararacussu venoms; nor to enrich commercial antivenoms against these Bothrops species. Fil: Rodríguez, Juan Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas Naturales y Agrimensura. Departamento de Bioquímica; Argentina Fil: de Marzi, Mauricio Cesar. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Maruñak, Silvana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina Fil: Teibler, Gladys Pamela. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina Fil: Acosta, Ofelia Cristina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina Fil: Malchiodi, Emilio Luis. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Estudios de la Inmunidad Humoral Prof. Ricardo A. Margni; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina Fil: Leiva, Laura Cristina Ana. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina |
description |
We examined the ability of IgG anti-crotalic PLA2 to cross-react with Bothrops spp. venoms, from snakes found in the northeast of Argentina. Immunoblotting and ELISA tests showed that IgG anti-crotalic PLA2 recognize antigens of bothropic venoms. Indirect hemolytic activity tests showed that the quantity of antibodies that neutralized 50% of Crotalus durissus terrificus venom (ED50: 2.1 mg IgG anti-crotalic PLA2/100 μg of venom) were also able to neutralize venom from other snakes in the following proportion: 34% of B. alternatus, 18% of B. diporus and 12% of B. jararacussu. Likewise, direct PLA2 activity neutralization tests showed a similar cross-neutralization pattern including 56% of B. alternatus, 29% of B. diporus and 30% of B. jararacussu. In addition, in a myotoxic activity neutralization test, measured by plasma activity of creatine kinase, 35% of B. alternatus venom and 26% of B. diporus venom were neutralized, while no neutralization was detected with B. jararacussu venom. This study presents original data concerning cross-reactions between bothropic venoms from Argentina and IgG anti-crotalic PLA2. Our results suggest that anti-crotalic PLA2 antibodies should not be used to neutralize PLA2 activity of B. alternatus, B. diporus and especially B. jararacussu venoms; nor to enrich commercial antivenoms against these Bothrops species. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-03 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/11336/61274 Rodríguez, Juan Pablo; de Marzi, Mauricio Cesar; Maruñak, Silvana; Teibler, Gladys Pamela; Acosta, Ofelia Cristina; et al.; IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina; Cevap-unesp; Journal Of Venomous Animals And Toxins Including Tropical Diseases; 15; 3; 3-2009; 460-478 1678-9199 CONICET Digital CONICET |
url |
http://hdl.handle.net/11336/61274 |
identifier_str_mv |
Rodríguez, Juan Pablo; de Marzi, Mauricio Cesar; Maruñak, Silvana; Teibler, Gladys Pamela; Acosta, Ofelia Cristina; et al.; IgG antibodies against phospholipase A2 from Crotalus durissus terrificus: Cross-reaction with venoms from Bothrops species from Argentina; Cevap-unesp; Journal Of Venomous Animals And Toxins Including Tropical Diseases; 15; 3; 3-2009; 460-478 1678-9199 CONICET Digital CONICET |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
info:eu-repo/semantics/altIdentifier/doi/10.1590/S1678-91992009000300008 info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/vqjcf4 |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc/2.5/ar/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc/2.5/ar/ |
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application/pdf application/pdf application/pdf application/pdf application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Cevap-unesp |
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Cevap-unesp |
dc.source.none.fl_str_mv |
reponame:CONICET Digital (CONICET) instname:Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) |
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CONICET Digital (CONICET) |
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Consejo Nacional de Investigaciones Científicas y Técnicas |
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CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas |
repository.mail.fl_str_mv |
dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar |
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1844614156823560192 |
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13.070432 |