Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells

Autores
Cholich, Luciana; Pistan, María; Torres, Ana; Hernández, David; Moro, Ramiro; Gómez, Tamara; Gardner, Dale; Bustillo, Soledad
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Cholich, Luciana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Cholich, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Pistan, María. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Pistan, María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Torres, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.
Fil: Hernández, David. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Moro, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Grupo de Investigaciones Biológicas y Moleculares; Argentina.
Fil: Gómez, Tamara. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Gardner, Dale. Servicio de Investigación Agrícola, Departamento de Agricultura de los Estados Unidos; Estados Unidos.
Fil: Bustillo, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Grupo de Investigaciones Biológicas y Moleculares; Argentina.
The consumption of Ipomoea carnea produces a neurological syndrome in animals. The toxic principles of I. carnea are the alkaloids swainsonine (SW) and calystegines B1, B2, B3 and C1. In this study, we investigated the cytotoxicity of an alkaloid extract of Ipomoea carnea (AEE) and natural swainsonine (SW) isolated from Astragalus lentiginosus (25–1000 μM of SW) for 48 h in a glioma cell line. Although the natural SW did not induce any changes in cell viability, the AEE exhibited a dose dependent cytotoxic effect and release of lactate dehydrogenase (LDH) indicative of cytolysis. In order to evaluate the morphological changes involved, cells were examined using phase contrast and fluorescence microscopy with acridine orange-ethidium bromide staining. The AEE caused a cell death compatible with necrosis, whereas exposure to 1000 μM of SW resulted in cytoplasmic vacuolation. Immunocytochemical studies revealed that astrocytes treated with 150 μM of AEE from I. carnea or 1000 μM of SW exhibited morphological characteristics of cell activation. These findings suggest that swainsonine would not be the only component present in the AEE of I. carnea responsible for in vitro cytotoxicity. Calystegines might also play a role in acting synergistically and triggering cell death through necrosis.
Fuente
Toxicon, 2023, vol. 235, p. 107325.
Materia
Ipomoea carnea extract
Swainsonine
Cytotoxicity
Glioma
C6 cell line
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
Institución
Universidad Nacional del Nordeste
OAI Identificador
oai:repositorio.unne.edu.ar:123456789/59354
Acceder
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oai_identifier_str oai:repositorio.unne.edu.ar:123456789/59354
network_acronym_str RIUNNE
repository_id_str 4871
network_name_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
spelling Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cellsCholich, LucianaPistan, MaríaTorres, AnaHernández, DavidMoro, RamiroGómez, TamaraGardner, DaleBustillo, SoledadIpomoea carnea extractSwainsonineCytotoxicityGliomaC6 cell lineFil: Cholich, Luciana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.Fil: Cholich, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Pistan, María. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.Fil: Pistan, María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Torres, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.Fil: Hernández, David. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.Fil: Moro, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Grupo de Investigaciones Biológicas y Moleculares; Argentina.Fil: Gómez, Tamara. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.Fil: Gardner, Dale. Servicio de Investigación Agrícola, Departamento de Agricultura de los Estados Unidos; Estados Unidos.Fil: Bustillo, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Grupo de Investigaciones Biológicas y Moleculares; Argentina.The consumption of Ipomoea carnea produces a neurological syndrome in animals. The toxic principles of I. carnea are the alkaloids swainsonine (SW) and calystegines B1, B2, B3 and C1. In this study, we investigated the cytotoxicity of an alkaloid extract of Ipomoea carnea (AEE) and natural swainsonine (SW) isolated from Astragalus lentiginosus (25–1000 μM of SW) for 48 h in a glioma cell line. Although the natural SW did not induce any changes in cell viability, the AEE exhibited a dose dependent cytotoxic effect and release of lactate dehydrogenase (LDH) indicative of cytolysis. In order to evaluate the morphological changes involved, cells were examined using phase contrast and fluorescence microscopy with acridine orange-ethidium bromide staining. The AEE caused a cell death compatible with necrosis, whereas exposure to 1000 μM of SW resulted in cytoplasmic vacuolation. Immunocytochemical studies revealed that astrocytes treated with 150 μM of AEE from I. carnea or 1000 μM of SW exhibited morphological characteristics of cell activation. These findings suggest that swainsonine would not be the only component present in the AEE of I. carnea responsible for in vitro cytotoxicity. Calystegines might also play a role in acting synergistically and triggering cell death through necrosis.Elsevier2023-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfp. 107325application/pdfCholich, Luciana, et al., 2023. Ipomoea carnea alkaloid extract vs swainsonine: a comparative study on cytotoxic activity against glial cells. Toxicon. Oxford: Elsevier, vol. 235, p. 107325. ISSN 1879-31500041-0101http://repositorio.unne.edu.ar/handle/123456789/59354Toxicon, 2023, vol. 235, p. 107325.reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)instname:Universidad Nacional del Nordesteenghttps://doi.org/10.1016/j.toxicon.2023.107325info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-nd/2.5/ar/Atribución-NoComercial-SinDerivadas 2.5 Argentina2026-01-08T11:17:29Zoai:repositorio.unne.edu.ar:123456789/59354instacron:UNNEInstitucionalhttp://repositorio.unne.edu.ar/Universidad públicaNo correspondehttp://repositorio.unne.edu.ar/oaiososa@bib.unne.edu.ar;sergio.alegria@unne.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:48712026-01-08 11:17:29.645Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordestefalse
dc.title.none.fl_str_mv Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells
title Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells
spellingShingle Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells
Cholich, Luciana
Ipomoea carnea extract
Swainsonine
Cytotoxicity
Glioma
C6 cell line
title_short Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells
title_full Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells
title_fullStr Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells
title_full_unstemmed Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells
title_sort Ipomoea carnea alkaloid extract vs swainsonine : a comparative study on cytotoxic activity against glial cells
dc.creator.none.fl_str_mv Cholich, Luciana
Pistan, María
Torres, Ana
Hernández, David
Moro, Ramiro
Gómez, Tamara
Gardner, Dale
Bustillo, Soledad
author Cholich, Luciana
author_facet Cholich, Luciana
Pistan, María
Torres, Ana
Hernández, David
Moro, Ramiro
Gómez, Tamara
Gardner, Dale
Bustillo, Soledad
author_role author
author2 Pistan, María
Torres, Ana
Hernández, David
Moro, Ramiro
Gómez, Tamara
Gardner, Dale
Bustillo, Soledad
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ipomoea carnea extract
Swainsonine
Cytotoxicity
Glioma
C6 cell line
topic Ipomoea carnea extract
Swainsonine
Cytotoxicity
Glioma
C6 cell line
dc.description.none.fl_txt_mv Fil: Cholich, Luciana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Cholich, Luciana. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Pistan, María. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Pistan, María. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Torres, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Química Básica y Aplicada del Nordeste Argentino; Argentina.
Fil: Hernández, David. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Moro, Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas. Grupo de Investigaciones Biológicas y Moleculares; Argentina.
Fil: Gómez, Tamara. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
Fil: Gardner, Dale. Servicio de Investigación Agrícola, Departamento de Agricultura de los Estados Unidos; Estados Unidos.
Fil: Bustillo, Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas. Grupo de Investigaciones Biológicas y Moleculares; Argentina.
The consumption of Ipomoea carnea produces a neurological syndrome in animals. The toxic principles of I. carnea are the alkaloids swainsonine (SW) and calystegines B1, B2, B3 and C1. In this study, we investigated the cytotoxicity of an alkaloid extract of Ipomoea carnea (AEE) and natural swainsonine (SW) isolated from Astragalus lentiginosus (25–1000 μM of SW) for 48 h in a glioma cell line. Although the natural SW did not induce any changes in cell viability, the AEE exhibited a dose dependent cytotoxic effect and release of lactate dehydrogenase (LDH) indicative of cytolysis. In order to evaluate the morphological changes involved, cells were examined using phase contrast and fluorescence microscopy with acridine orange-ethidium bromide staining. The AEE caused a cell death compatible with necrosis, whereas exposure to 1000 μM of SW resulted in cytoplasmic vacuolation. Immunocytochemical studies revealed that astrocytes treated with 150 μM of AEE from I. carnea or 1000 μM of SW exhibited morphological characteristics of cell activation. These findings suggest that swainsonine would not be the only component present in the AEE of I. carnea responsible for in vitro cytotoxicity. Calystegines might also play a role in acting synergistically and triggering cell death through necrosis.
description Fil: Cholich, Luciana. Universidad Nacional del Nordeste. Facultad de Ciencias Veterinarias; Argentina.
publishDate 2023
dc.date.none.fl_str_mv 2023-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv Cholich, Luciana, et al., 2023. Ipomoea carnea alkaloid extract vs swainsonine: a comparative study on cytotoxic activity against glial cells. Toxicon. Oxford: Elsevier, vol. 235, p. 107325. ISSN 1879-3150
0041-0101
http://repositorio.unne.edu.ar/handle/123456789/59354
identifier_str_mv Cholich, Luciana, et al., 2023. Ipomoea carnea alkaloid extract vs swainsonine: a comparative study on cytotoxic activity against glial cells. Toxicon. Oxford: Elsevier, vol. 235, p. 107325. ISSN 1879-3150
0041-0101
url http://repositorio.unne.edu.ar/handle/123456789/59354
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.toxicon.2023.107325
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Atribución-NoComercial-SinDerivadas 2.5 Argentina
dc.format.none.fl_str_mv application/pdf
p. 107325
application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Toxicon, 2023, vol. 235, p. 107325.
reponame:Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname:Universidad Nacional del Nordeste
reponame_str Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
collection Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE)
instname_str Universidad Nacional del Nordeste
repository.name.fl_str_mv Repositorio Institucional de la Universidad Nacional del Nordeste (UNNE) - Universidad Nacional del Nordeste
repository.mail.fl_str_mv ososa@bib.unne.edu.ar;sergio.alegria@unne.edu.ar
_version_ 1853761397399224320
score 13.25844

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