TRPC channels are not required for graded persistent activity in entorhinal cortex neurons

Autores
Egorov, Alexei V.; Schumacher, Dagmar; Medert, Rebekka; Birnbaumer, Lutz; Freichel, Marc; Draguhn, Andreas
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Egorov, Alexei V. Heidelberg University. Institute of Physiology and Pathophysiology; Alemania
Fil: Schumacher, Dagmar. Heidelberg University. Institute of Pharmacology; Alemania
Fil: Medert, Rebekka. Heidelberg University. Institute of Pharmacology; Alemania
Fil: Birnbaumer, Lutz. National Institute of Environmental Health Sciences. Neurobiology Laboratory; Estados Unidos
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Freichel, Marc. Heidelberg University. Institute of Pharmacology; Alemania
Fil: Draguhn, Andreas. Heidelberg University. Institute of Physiology and Pathophysiology; Alemania
Abstract: Adaptive behavior requires the transient storage of information beyond the physical presence of external stimuli. This short-lasting form of memory involves sustained ("persistent") neuronal firing which may be generated by cell-autonomous biophysical properties of neurons or/and neural circuit dynamics. A number of studies from brain slices reports intrinsically generated persistent firing in cortical excitatory neurons following suprathreshold depolarization by intracellular current injection. In layer V (LV) neurons of the medial entorhinal cortex (mEC) persistent firing depends on the activation of cholinergic muscarinic receptors and is mediated by a calcium-activated nonselective cation current (ICAN ). The molecular identity of this conductance remains, however, unknown. Recently, it has been suggested that the underlying ion channels belong to the canonical transient receptor potential (TRPC) channel family and include heterotetramers of TRPC1/5, TRPC1/4, and/or TRPC1/4/5 channels. While this suggestion was based on pharmacological experiments and on effects of TRP-interacting peptides, an unambiguous proof based on TRPC channel-depleted animals is pending. Here, we used two different lines of TRPC channel knockout mice, either lacking TRPC1-, TRPC4-, and TRPC5-containing channels or lacking all seven members of the TRPC family. We report unchanged persistent activity in mEC LV neurons in these animals, ruling out that muscarinic-dependent persistent activity depends on TRPC channels.
Fuente
Hippocampus. 2019, 29(11)
Materia
FUNCION COGNITIVA
NEURONAS
CORTEZA CEREBRAL
NEUROTRANSMISORES
MEMORIA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/9470

id RIUCA_d2120882c969b2a709684216f3008508
oai_identifier_str oai:ucacris:123456789/9470
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling TRPC channels are not required for graded persistent activity in entorhinal cortex neuronsEgorov, Alexei V.Schumacher, DagmarMedert, RebekkaBirnbaumer, LutzFreichel, MarcDraguhn, AndreasFUNCION COGNITIVANEURONASCORTEZA CEREBRALNEUROTRANSMISORESMEMORIAFil: Egorov, Alexei V. Heidelberg University. Institute of Physiology and Pathophysiology; AlemaniaFil: Schumacher, Dagmar. Heidelberg University. Institute of Pharmacology; AlemaniaFil: Medert, Rebekka. Heidelberg University. Institute of Pharmacology; AlemaniaFil: Birnbaumer, Lutz. National Institute of Environmental Health Sciences. Neurobiology Laboratory; Estados UnidosFil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Freichel, Marc. Heidelberg University. Institute of Pharmacology; AlemaniaFil: Draguhn, Andreas. Heidelberg University. Institute of Physiology and Pathophysiology; AlemaniaAbstract: Adaptive behavior requires the transient storage of information beyond the physical presence of external stimuli. This short-lasting form of memory involves sustained ("persistent") neuronal firing which may be generated by cell-autonomous biophysical properties of neurons or/and neural circuit dynamics. A number of studies from brain slices reports intrinsically generated persistent firing in cortical excitatory neurons following suprathreshold depolarization by intracellular current injection. In layer V (LV) neurons of the medial entorhinal cortex (mEC) persistent firing depends on the activation of cholinergic muscarinic receptors and is mediated by a calcium-activated nonselective cation current (ICAN ). The molecular identity of this conductance remains, however, unknown. Recently, it has been suggested that the underlying ion channels belong to the canonical transient receptor potential (TRPC) channel family and include heterotetramers of TRPC1/5, TRPC1/4, and/or TRPC1/4/5 channels. While this suggestion was based on pharmacological experiments and on effects of TRP-interacting peptides, an unambiguous proof based on TRPC channel-depleted animals is pending. Here, we used two different lines of TRPC channel knockout mice, either lacking TRPC1-, TRPC4-, and TRPC5-containing channels or lacking all seven members of the TRPC family. We report unchanged persistent activity in mEC LV neurons in these animals, ruling out that muscarinic-dependent persistent activity depends on TRPC channels.Wiley2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/94701050-9631 (impreso)1098-1063 (on line)10.1002/hipo.2309431002217Egorov, A., Schumacher, D., Medert, R., et al. TRPC channels are not required for graded persistent activity in entorhinal cortex neurons. Hippocampus. 2019, 29(11). doi:10.1002/hipo.23094. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/9470Hippocampus. 2019, 29(11)reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:57:06Zoai:ucacris:123456789/9470instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:57:07.208Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
title TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
spellingShingle TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
Egorov, Alexei V.
FUNCION COGNITIVA
NEURONAS
CORTEZA CEREBRAL
NEUROTRANSMISORES
MEMORIA
title_short TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
title_full TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
title_fullStr TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
title_full_unstemmed TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
title_sort TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
dc.creator.none.fl_str_mv Egorov, Alexei V.
Schumacher, Dagmar
Medert, Rebekka
Birnbaumer, Lutz
Freichel, Marc
Draguhn, Andreas
author Egorov, Alexei V.
author_facet Egorov, Alexei V.
Schumacher, Dagmar
Medert, Rebekka
Birnbaumer, Lutz
Freichel, Marc
Draguhn, Andreas
author_role author
author2 Schumacher, Dagmar
Medert, Rebekka
Birnbaumer, Lutz
Freichel, Marc
Draguhn, Andreas
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv FUNCION COGNITIVA
NEURONAS
CORTEZA CEREBRAL
NEUROTRANSMISORES
MEMORIA
topic FUNCION COGNITIVA
NEURONAS
CORTEZA CEREBRAL
NEUROTRANSMISORES
MEMORIA
dc.description.none.fl_txt_mv Fil: Egorov, Alexei V. Heidelberg University. Institute of Physiology and Pathophysiology; Alemania
Fil: Schumacher, Dagmar. Heidelberg University. Institute of Pharmacology; Alemania
Fil: Medert, Rebekka. Heidelberg University. Institute of Pharmacology; Alemania
Fil: Birnbaumer, Lutz. National Institute of Environmental Health Sciences. Neurobiology Laboratory; Estados Unidos
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Freichel, Marc. Heidelberg University. Institute of Pharmacology; Alemania
Fil: Draguhn, Andreas. Heidelberg University. Institute of Physiology and Pathophysiology; Alemania
Abstract: Adaptive behavior requires the transient storage of information beyond the physical presence of external stimuli. This short-lasting form of memory involves sustained ("persistent") neuronal firing which may be generated by cell-autonomous biophysical properties of neurons or/and neural circuit dynamics. A number of studies from brain slices reports intrinsically generated persistent firing in cortical excitatory neurons following suprathreshold depolarization by intracellular current injection. In layer V (LV) neurons of the medial entorhinal cortex (mEC) persistent firing depends on the activation of cholinergic muscarinic receptors and is mediated by a calcium-activated nonselective cation current (ICAN ). The molecular identity of this conductance remains, however, unknown. Recently, it has been suggested that the underlying ion channels belong to the canonical transient receptor potential (TRPC) channel family and include heterotetramers of TRPC1/5, TRPC1/4, and/or TRPC1/4/5 channels. While this suggestion was based on pharmacological experiments and on effects of TRP-interacting peptides, an unambiguous proof based on TRPC channel-depleted animals is pending. Here, we used two different lines of TRPC channel knockout mice, either lacking TRPC1-, TRPC4-, and TRPC5-containing channels or lacking all seven members of the TRPC family. We report unchanged persistent activity in mEC LV neurons in these animals, ruling out that muscarinic-dependent persistent activity depends on TRPC channels.
description Fil: Egorov, Alexei V. Heidelberg University. Institute of Physiology and Pathophysiology; Alemania
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/9470
1050-9631 (impreso)
1098-1063 (on line)
10.1002/hipo.23094
31002217
Egorov, A., Schumacher, D., Medert, R., et al. TRPC channels are not required for graded persistent activity in entorhinal cortex neurons. Hippocampus. 2019, 29(11). doi:10.1002/hipo.23094. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/9470
url https://repositorio.uca.edu.ar/handle/123456789/9470
identifier_str_mv 1050-9631 (impreso)
1098-1063 (on line)
10.1002/hipo.23094
31002217
Egorov, A., Schumacher, D., Medert, R., et al. TRPC channels are not required for graded persistent activity in entorhinal cortex neurons. Hippocampus. 2019, 29(11). doi:10.1002/hipo.23094. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/9470
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Hippocampus. 2019, 29(11)
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
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