TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
- Autores
- Egorov, Alexei V.; Schumacher, Dagmar; Medert, Rebekka; Birnbaumer, Lutz; Freichel, Marc; Draguhn, Andreas
- Año de publicación
- 2019
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- Fil: Egorov, Alexei V. Heidelberg University. Institute of Physiology and Pathophysiology; Alemania
Fil: Schumacher, Dagmar. Heidelberg University. Institute of Pharmacology; Alemania
Fil: Medert, Rebekka. Heidelberg University. Institute of Pharmacology; Alemania
Fil: Birnbaumer, Lutz. National Institute of Environmental Health Sciences. Neurobiology Laboratory; Estados Unidos
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Freichel, Marc. Heidelberg University. Institute of Pharmacology; Alemania
Fil: Draguhn, Andreas. Heidelberg University. Institute of Physiology and Pathophysiology; Alemania
Abstract: Adaptive behavior requires the transient storage of information beyond the physical presence of external stimuli. This short-lasting form of memory involves sustained ("persistent") neuronal firing which may be generated by cell-autonomous biophysical properties of neurons or/and neural circuit dynamics. A number of studies from brain slices reports intrinsically generated persistent firing in cortical excitatory neurons following suprathreshold depolarization by intracellular current injection. In layer V (LV) neurons of the medial entorhinal cortex (mEC) persistent firing depends on the activation of cholinergic muscarinic receptors and is mediated by a calcium-activated nonselective cation current (ICAN ). The molecular identity of this conductance remains, however, unknown. Recently, it has been suggested that the underlying ion channels belong to the canonical transient receptor potential (TRPC) channel family and include heterotetramers of TRPC1/5, TRPC1/4, and/or TRPC1/4/5 channels. While this suggestion was based on pharmacological experiments and on effects of TRP-interacting peptides, an unambiguous proof based on TRPC channel-depleted animals is pending. Here, we used two different lines of TRPC channel knockout mice, either lacking TRPC1-, TRPC4-, and TRPC5-containing channels or lacking all seven members of the TRPC family. We report unchanged persistent activity in mEC LV neurons in these animals, ruling out that muscarinic-dependent persistent activity depends on TRPC channels. - Fuente
- Hippocampus. 2019, 29(11)
- Materia
-
FUNCION COGNITIVA
NEURONAS
CORTEZA CEREBRAL
NEUROTRANSMISORES
MEMORIA - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Pontificia Universidad Católica Argentina
- OAI Identificador
- oai:ucacris:123456789/9470
Ver los metadatos del registro completo
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spelling |
TRPC channels are not required for graded persistent activity in entorhinal cortex neuronsEgorov, Alexei V.Schumacher, DagmarMedert, RebekkaBirnbaumer, LutzFreichel, MarcDraguhn, AndreasFUNCION COGNITIVANEURONASCORTEZA CEREBRALNEUROTRANSMISORESMEMORIAFil: Egorov, Alexei V. Heidelberg University. Institute of Physiology and Pathophysiology; AlemaniaFil: Schumacher, Dagmar. Heidelberg University. Institute of Pharmacology; AlemaniaFil: Medert, Rebekka. Heidelberg University. Institute of Pharmacology; AlemaniaFil: Birnbaumer, Lutz. National Institute of Environmental Health Sciences. Neurobiology Laboratory; Estados UnidosFil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Freichel, Marc. Heidelberg University. Institute of Pharmacology; AlemaniaFil: Draguhn, Andreas. Heidelberg University. Institute of Physiology and Pathophysiology; AlemaniaAbstract: Adaptive behavior requires the transient storage of information beyond the physical presence of external stimuli. This short-lasting form of memory involves sustained ("persistent") neuronal firing which may be generated by cell-autonomous biophysical properties of neurons or/and neural circuit dynamics. A number of studies from brain slices reports intrinsically generated persistent firing in cortical excitatory neurons following suprathreshold depolarization by intracellular current injection. In layer V (LV) neurons of the medial entorhinal cortex (mEC) persistent firing depends on the activation of cholinergic muscarinic receptors and is mediated by a calcium-activated nonselective cation current (ICAN ). The molecular identity of this conductance remains, however, unknown. Recently, it has been suggested that the underlying ion channels belong to the canonical transient receptor potential (TRPC) channel family and include heterotetramers of TRPC1/5, TRPC1/4, and/or TRPC1/4/5 channels. While this suggestion was based on pharmacological experiments and on effects of TRP-interacting peptides, an unambiguous proof based on TRPC channel-depleted animals is pending. Here, we used two different lines of TRPC channel knockout mice, either lacking TRPC1-, TRPC4-, and TRPC5-containing channels or lacking all seven members of the TRPC family. We report unchanged persistent activity in mEC LV neurons in these animals, ruling out that muscarinic-dependent persistent activity depends on TRPC channels.Wiley2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/94701050-9631 (impreso)1098-1063 (on line)10.1002/hipo.2309431002217Egorov, A., Schumacher, D., Medert, R., et al. TRPC channels are not required for graded persistent activity in entorhinal cortex neurons. Hippocampus. 2019, 29(11). doi:10.1002/hipo.23094. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/9470Hippocampus. 2019, 29(11)reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:57:06Zoai:ucacris:123456789/9470instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:57:07.208Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse |
dc.title.none.fl_str_mv |
TRPC channels are not required for graded persistent activity in entorhinal cortex neurons |
title |
TRPC channels are not required for graded persistent activity in entorhinal cortex neurons |
spellingShingle |
TRPC channels are not required for graded persistent activity in entorhinal cortex neurons Egorov, Alexei V. FUNCION COGNITIVA NEURONAS CORTEZA CEREBRAL NEUROTRANSMISORES MEMORIA |
title_short |
TRPC channels are not required for graded persistent activity in entorhinal cortex neurons |
title_full |
TRPC channels are not required for graded persistent activity in entorhinal cortex neurons |
title_fullStr |
TRPC channels are not required for graded persistent activity in entorhinal cortex neurons |
title_full_unstemmed |
TRPC channels are not required for graded persistent activity in entorhinal cortex neurons |
title_sort |
TRPC channels are not required for graded persistent activity in entorhinal cortex neurons |
dc.creator.none.fl_str_mv |
Egorov, Alexei V. Schumacher, Dagmar Medert, Rebekka Birnbaumer, Lutz Freichel, Marc Draguhn, Andreas |
author |
Egorov, Alexei V. |
author_facet |
Egorov, Alexei V. Schumacher, Dagmar Medert, Rebekka Birnbaumer, Lutz Freichel, Marc Draguhn, Andreas |
author_role |
author |
author2 |
Schumacher, Dagmar Medert, Rebekka Birnbaumer, Lutz Freichel, Marc Draguhn, Andreas |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
FUNCION COGNITIVA NEURONAS CORTEZA CEREBRAL NEUROTRANSMISORES MEMORIA |
topic |
FUNCION COGNITIVA NEURONAS CORTEZA CEREBRAL NEUROTRANSMISORES MEMORIA |
dc.description.none.fl_txt_mv |
Fil: Egorov, Alexei V. Heidelberg University. Institute of Physiology and Pathophysiology; Alemania Fil: Schumacher, Dagmar. Heidelberg University. Institute of Pharmacology; Alemania Fil: Medert, Rebekka. Heidelberg University. Institute of Pharmacology; Alemania Fil: Birnbaumer, Lutz. National Institute of Environmental Health Sciences. Neurobiology Laboratory; Estados Unidos Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina Fil: Birnbaumer, Lutz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Freichel, Marc. Heidelberg University. Institute of Pharmacology; Alemania Fil: Draguhn, Andreas. Heidelberg University. Institute of Physiology and Pathophysiology; Alemania Abstract: Adaptive behavior requires the transient storage of information beyond the physical presence of external stimuli. This short-lasting form of memory involves sustained ("persistent") neuronal firing which may be generated by cell-autonomous biophysical properties of neurons or/and neural circuit dynamics. A number of studies from brain slices reports intrinsically generated persistent firing in cortical excitatory neurons following suprathreshold depolarization by intracellular current injection. In layer V (LV) neurons of the medial entorhinal cortex (mEC) persistent firing depends on the activation of cholinergic muscarinic receptors and is mediated by a calcium-activated nonselective cation current (ICAN ). The molecular identity of this conductance remains, however, unknown. Recently, it has been suggested that the underlying ion channels belong to the canonical transient receptor potential (TRPC) channel family and include heterotetramers of TRPC1/5, TRPC1/4, and/or TRPC1/4/5 channels. While this suggestion was based on pharmacological experiments and on effects of TRP-interacting peptides, an unambiguous proof based on TRPC channel-depleted animals is pending. Here, we used two different lines of TRPC channel knockout mice, either lacking TRPC1-, TRPC4-, and TRPC5-containing channels or lacking all seven members of the TRPC family. We report unchanged persistent activity in mEC LV neurons in these animals, ruling out that muscarinic-dependent persistent activity depends on TRPC channels. |
description |
Fil: Egorov, Alexei V. Heidelberg University. Institute of Physiology and Pathophysiology; Alemania |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://repositorio.uca.edu.ar/handle/123456789/9470 1050-9631 (impreso) 1098-1063 (on line) 10.1002/hipo.23094 31002217 Egorov, A., Schumacher, D., Medert, R., et al. TRPC channels are not required for graded persistent activity in entorhinal cortex neurons. Hippocampus. 2019, 29(11). doi:10.1002/hipo.23094. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/9470 |
url |
https://repositorio.uca.edu.ar/handle/123456789/9470 |
identifier_str_mv |
1050-9631 (impreso) 1098-1063 (on line) 10.1002/hipo.23094 31002217 Egorov, A., Schumacher, D., Medert, R., et al. TRPC channels are not required for graded persistent activity in entorhinal cortex neurons. Hippocampus. 2019, 29(11). doi:10.1002/hipo.23094. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/9470 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
Hippocampus. 2019, 29(11) reponame:Repositorio Institucional (UCA) instname:Pontificia Universidad Católica Argentina |
reponame_str |
Repositorio Institucional (UCA) |
collection |
Repositorio Institucional (UCA) |
instname_str |
Pontificia Universidad Católica Argentina |
repository.name.fl_str_mv |
Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina |
repository.mail.fl_str_mv |
claudia_fernandez@uca.edu.ar |
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1836638349784252416 |
score |
13.13397 |