TRPC channels are not required for graded persistent activity in entorhinal cortex neurons

Autores
Egorov, Alexei V.; Schumacher, Dagmar; Medert, Rebekka; Birnbaumer, Lutz; Freichel, Marc; Draguhn, Andreas
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Adaptive behavior requires the transient storage of information beyond the physical presence of external stimuli. This short-lasting form of memory involves sustained (“persistent”) neuronal firing which may be generated by cell-autonomous biophysical properties of neurons or/and neural circuit dynamics. A number of studies from brain slices reports intrinsically generated persistent firing in cortical excitatory neurons following suprathreshold depolarization by intracellular current injection. In layer V (LV) neurons of the medial entorhinal cortex (mEC) persistent firing depends on the activation of cholinergic muscarinic receptors and is mediated by a calcium-activated nonselective cation current (ICAN). The molecular identity of this conductance remains, however, unknown. Recently, it has been suggested that the underlying ion channels belong to the canonical transient receptor potential (TRPC) channel family and include heterotetramers of TRPC1/5, TRPC1/4, and/or TRPC1/4/5 channels. While this suggestion was based on pharmacological experiments and on effects of TRP-interacting peptides, an unambiguous proof based on TRPC channel-depleted animals is pending. Here, we used two different lines of TRPC channel knockout mice, either lacking TRPC1-, TRPC4-, and TRPC5-containing channels or lacking all seven members of the TRPC family. We report unchanged persistent activity in mEC LV neurons in these animals, ruling out that muscarinic-dependent persistent activity depends on TRPC channels.
Fil: Egorov, Alexei V.. Universität Heidelberg; Alemania
Fil: Schumacher, Dagmar. Universität Heidelberg; Alemania
Fil: Medert, Rebekka. Universität Heidelberg; Alemania
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Freichel, Marc. Universität Heidelberg; Alemania
Fil: Draguhn, Andreas. Universität Heidelberg; Alemania
Materia
HEPTA-TRPC KO MICE
MUSCARINIC
PERSISTENT FIRING
TRPC1/4/5 KO MICE
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/120563
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling TRPC channels are not required for graded persistent activity in entorhinal cortex neuronsEgorov, Alexei V.Schumacher, DagmarMedert, RebekkaBirnbaumer, LutzFreichel, MarcDraguhn, AndreasHEPTA-TRPC KO MICEMUSCARINICPERSISTENT FIRINGTRPC1/4/5 KO MICEhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Adaptive behavior requires the transient storage of information beyond the physical presence of external stimuli. This short-lasting form of memory involves sustained (“persistent”) neuronal firing which may be generated by cell-autonomous biophysical properties of neurons or/and neural circuit dynamics. A number of studies from brain slices reports intrinsically generated persistent firing in cortical excitatory neurons following suprathreshold depolarization by intracellular current injection. In layer V (LV) neurons of the medial entorhinal cortex (mEC) persistent firing depends on the activation of cholinergic muscarinic receptors and is mediated by a calcium-activated nonselective cation current (ICAN). The molecular identity of this conductance remains, however, unknown. Recently, it has been suggested that the underlying ion channels belong to the canonical transient receptor potential (TRPC) channel family and include heterotetramers of TRPC1/5, TRPC1/4, and/or TRPC1/4/5 channels. While this suggestion was based on pharmacological experiments and on effects of TRP-interacting peptides, an unambiguous proof based on TRPC channel-depleted animals is pending. Here, we used two different lines of TRPC channel knockout mice, either lacking TRPC1-, TRPC4-, and TRPC5-containing channels or lacking all seven members of the TRPC family. We report unchanged persistent activity in mEC LV neurons in these animals, ruling out that muscarinic-dependent persistent activity depends on TRPC channels.Fil: Egorov, Alexei V.. Universität Heidelberg; AlemaniaFil: Schumacher, Dagmar. Universität Heidelberg; AlemaniaFil: Medert, Rebekka. Universität Heidelberg; AlemaniaFil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; ArgentinaFil: Freichel, Marc. Universität Heidelberg; AlemaniaFil: Draguhn, Andreas. Universität Heidelberg; AlemaniaVeterinary and Human Toxicology2019-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/120563Egorov, Alexei V.; Schumacher, Dagmar; Medert, Rebekka; Birnbaumer, Lutz; Freichel, Marc; et al.; TRPC channels are not required for graded persistent activity in entorhinal cortex neurons; Veterinary and Human Toxicology; Hippocampus; 29; 11; 11-2019; 1038-10481050-9631CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1002/hipo.23094info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/epdf/10.1002/hipo.23094info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6791731/info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:58:31Zoai:ri.conicet.gov.ar:11336/120563instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:58:31.95CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
title TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
spellingShingle TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
Egorov, Alexei V.
HEPTA-TRPC KO MICE
MUSCARINIC
PERSISTENT FIRING
TRPC1/4/5 KO MICE
title_short TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
title_full TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
title_fullStr TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
title_full_unstemmed TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
title_sort TRPC channels are not required for graded persistent activity in entorhinal cortex neurons
dc.creator.none.fl_str_mv Egorov, Alexei V.
Schumacher, Dagmar
Medert, Rebekka
Birnbaumer, Lutz
Freichel, Marc
Draguhn, Andreas
author Egorov, Alexei V.
author_facet Egorov, Alexei V.
Schumacher, Dagmar
Medert, Rebekka
Birnbaumer, Lutz
Freichel, Marc
Draguhn, Andreas
author_role author
author2 Schumacher, Dagmar
Medert, Rebekka
Birnbaumer, Lutz
Freichel, Marc
Draguhn, Andreas
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv HEPTA-TRPC KO MICE
MUSCARINIC
PERSISTENT FIRING
TRPC1/4/5 KO MICE
topic HEPTA-TRPC KO MICE
MUSCARINIC
PERSISTENT FIRING
TRPC1/4/5 KO MICE
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Adaptive behavior requires the transient storage of information beyond the physical presence of external stimuli. This short-lasting form of memory involves sustained (“persistent”) neuronal firing which may be generated by cell-autonomous biophysical properties of neurons or/and neural circuit dynamics. A number of studies from brain slices reports intrinsically generated persistent firing in cortical excitatory neurons following suprathreshold depolarization by intracellular current injection. In layer V (LV) neurons of the medial entorhinal cortex (mEC) persistent firing depends on the activation of cholinergic muscarinic receptors and is mediated by a calcium-activated nonselective cation current (ICAN). The molecular identity of this conductance remains, however, unknown. Recently, it has been suggested that the underlying ion channels belong to the canonical transient receptor potential (TRPC) channel family and include heterotetramers of TRPC1/5, TRPC1/4, and/or TRPC1/4/5 channels. While this suggestion was based on pharmacological experiments and on effects of TRP-interacting peptides, an unambiguous proof based on TRPC channel-depleted animals is pending. Here, we used two different lines of TRPC channel knockout mice, either lacking TRPC1-, TRPC4-, and TRPC5-containing channels or lacking all seven members of the TRPC family. We report unchanged persistent activity in mEC LV neurons in these animals, ruling out that muscarinic-dependent persistent activity depends on TRPC channels.
Fil: Egorov, Alexei V.. Universität Heidelberg; Alemania
Fil: Schumacher, Dagmar. Universität Heidelberg; Alemania
Fil: Medert, Rebekka. Universität Heidelberg; Alemania
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires". Instituto de Investigaciones Biomédicas. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas; Argentina
Fil: Freichel, Marc. Universität Heidelberg; Alemania
Fil: Draguhn, Andreas. Universität Heidelberg; Alemania
description Adaptive behavior requires the transient storage of information beyond the physical presence of external stimuli. This short-lasting form of memory involves sustained (“persistent”) neuronal firing which may be generated by cell-autonomous biophysical properties of neurons or/and neural circuit dynamics. A number of studies from brain slices reports intrinsically generated persistent firing in cortical excitatory neurons following suprathreshold depolarization by intracellular current injection. In layer V (LV) neurons of the medial entorhinal cortex (mEC) persistent firing depends on the activation of cholinergic muscarinic receptors and is mediated by a calcium-activated nonselective cation current (ICAN). The molecular identity of this conductance remains, however, unknown. Recently, it has been suggested that the underlying ion channels belong to the canonical transient receptor potential (TRPC) channel family and include heterotetramers of TRPC1/5, TRPC1/4, and/or TRPC1/4/5 channels. While this suggestion was based on pharmacological experiments and on effects of TRP-interacting peptides, an unambiguous proof based on TRPC channel-depleted animals is pending. Here, we used two different lines of TRPC channel knockout mice, either lacking TRPC1-, TRPC4-, and TRPC5-containing channels or lacking all seven members of the TRPC family. We report unchanged persistent activity in mEC LV neurons in these animals, ruling out that muscarinic-dependent persistent activity depends on TRPC channels.
publishDate 2019
dc.date.none.fl_str_mv 2019-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/120563
Egorov, Alexei V.; Schumacher, Dagmar; Medert, Rebekka; Birnbaumer, Lutz; Freichel, Marc; et al.; TRPC channels are not required for graded persistent activity in entorhinal cortex neurons; Veterinary and Human Toxicology; Hippocampus; 29; 11; 11-2019; 1038-1048
1050-9631
CONICET Digital
CONICET
url http://hdl.handle.net/11336/120563
identifier_str_mv Egorov, Alexei V.; Schumacher, Dagmar; Medert, Rebekka; Birnbaumer, Lutz; Freichel, Marc; et al.; TRPC channels are not required for graded persistent activity in entorhinal cortex neurons; Veterinary and Human Toxicology; Hippocampus; 29; 11; 11-2019; 1038-1048
1050-9631
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1002/hipo.23094
info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/epdf/10.1002/hipo.23094
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6791731/
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-nd/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Veterinary and Human Toxicology
publisher.none.fl_str_mv Veterinary and Human Toxicology
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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