TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo
- Autores
- Belkacemi, Thabet; Niermann, Alexander; Hofmann, Laura; Wissenbach, Ulrich; Birnbaumer, Lutz; Leidinger, Petra; Backes, Christina; Meese, Eckart; Keller, Andreas; Bai, Xianshu; Scheller, Anja; Kirchhoff, Frank; Philipp, Stephan E.; Weissgerber, Petra; Flockerzi, Veit; Beck, Andreas
- Año de publicación
- 2017
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión aceptada
- Descripción
- Fil: Belkacemi, Thabet. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania
Fil: Niermann, Alexander. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania
Fil: Hofmann, Laura. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania
Fil: Wissenbach, Ulrich. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania
Fil: Birnbaumer, Lutz. National Institute of Environmental Health Sciences. Research Triangle Park. Neurobiology Laboratory; Estados Unidos
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Leidinger, Petra. Universität des Saarlandes. Institut für Humangenetik; Alemania
Fil: Backes, Christina. Universität des Saarlandes. Klinische Bioinformatik; Alemania
Fil: Meese, Eckart. Universität des Saarlandes. Institut für Humangenetik; Alemania
Fil: Keller, Andreas. Universität des Saarlandes. Klinische Bioinformatik; Alemania
Fil: Bai, Xianshu. Universität des Saarlandes. Molekulare Physiologie; Alemania
Fil: Scheller, Anja. Universität des Saarlandes. Molekulare Physiologie; Alemania
Fil: Kirchhoff, Frank. Universität des Saarlandes. Molekulare Physiologie; Alemania
Fil: Philipp, Stephan E. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania
Fil: Weissgerber, Petra. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania
Fil: Flockerzi, Veit. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania
Fil: Beck, Andreas. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania
Fil: Beck, Andreas. Universität des Saarlandes. Molekulare Physiologie; Alemania
Abstract: Following brain injury astrocytes change into a reactive state, proliferate and grow into the site of lesion, a process called astrogliosis, initiated and regulated by changes in cytoplasmic Ca2+ . Transient receptor potential canonical (TRPC) channels may contribute to Ca2+ influx but their presence and possible function in astrocytes is not known. By RT-PCR and RNA sequencing we identified transcripts of Trpc1, Trpc2, Trpc3, and Trpc4 in FACS-sorted glutamate aspartate transporter (GLAST)-positive cultured mouse cortical astrocytes and subcloned full-length Trpc1 and Trpc3 cDNAs from these cells. Ca2+ entry in cortical astrocytes depended on TRPC3 and was increased in the absence of Trpc1. After co-expression of Trpc1 and Trpc3 in HEK-293 cells both proteins co-immunoprecipitate and form functional heteromeric channels, with TRPC1 reducing TRPC3 activity. In vitro, lack of Trpc3 reduced astrocyte proliferation and migration whereas the TRPC3 gain-of-function moonwalker mutation and Trpc1 deficiency increased astrocyte migration. In vivo, astrogliosis and cortex edema following stab wound injury were reduced in Trpc3-/- but increased in Trpc1-/- mice. In summary, our results show a decisive contribution of TRPC3 to astrocyte Ca2+ signaling, which is even augmented in the absence of Trpc1, in particular following brain injury. Targeted therapies to reduce TRPC3 channel activity in astrocytes might therefore be beneficial in traumatic brain injury. - Fuente
- Glia. 2017;65(9):1535-1549
- Materia
-
TEJIDO NERVIOSO
CELULAS
CEREBRO
CORTEZA CEREBRAL
HERIDAS - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- https://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Pontificia Universidad Católica Argentina
- OAI Identificador
- oai:ucacris:123456789/8724
Ver los metadatos del registro completo
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network_name_str |
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spelling |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivoBelkacemi, ThabetNiermann, AlexanderHofmann, LauraWissenbach, UlrichBirnbaumer, LutzLeidinger, PetraBackes, ChristinaMeese, EckartKeller, AndreasBai, XianshuScheller, AnjaKirchhoff, FrankPhilipp, Stephan E.Weissgerber, PetraFlockerzi, VeitBeck, AndreasTEJIDO NERVIOSOCELULASCEREBROCORTEZA CEREBRALHERIDASFil: Belkacemi, Thabet. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; AlemaniaFil: Niermann, Alexander. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; AlemaniaFil: Hofmann, Laura. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; AlemaniaFil: Wissenbach, Ulrich. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; AlemaniaFil: Birnbaumer, Lutz. National Institute of Environmental Health Sciences. Research Triangle Park. Neurobiology Laboratory; Estados UnidosFil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; ArgentinaFil: Leidinger, Petra. Universität des Saarlandes. Institut für Humangenetik; AlemaniaFil: Backes, Christina. Universität des Saarlandes. Klinische Bioinformatik; AlemaniaFil: Meese, Eckart. Universität des Saarlandes. Institut für Humangenetik; AlemaniaFil: Keller, Andreas. Universität des Saarlandes. Klinische Bioinformatik; AlemaniaFil: Bai, Xianshu. Universität des Saarlandes. Molekulare Physiologie; AlemaniaFil: Scheller, Anja. Universität des Saarlandes. Molekulare Physiologie; AlemaniaFil: Kirchhoff, Frank. Universität des Saarlandes. Molekulare Physiologie; AlemaniaFil: Philipp, Stephan E. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; AlemaniaFil: Weissgerber, Petra. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; AlemaniaFil: Flockerzi, Veit. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; AlemaniaFil: Beck, Andreas. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; AlemaniaFil: Beck, Andreas. Universität des Saarlandes. Molekulare Physiologie; AlemaniaAbstract: Following brain injury astrocytes change into a reactive state, proliferate and grow into the site of lesion, a process called astrogliosis, initiated and regulated by changes in cytoplasmic Ca2+ . Transient receptor potential canonical (TRPC) channels may contribute to Ca2+ influx but their presence and possible function in astrocytes is not known. By RT-PCR and RNA sequencing we identified transcripts of Trpc1, Trpc2, Trpc3, and Trpc4 in FACS-sorted glutamate aspartate transporter (GLAST)-positive cultured mouse cortical astrocytes and subcloned full-length Trpc1 and Trpc3 cDNAs from these cells. Ca2+ entry in cortical astrocytes depended on TRPC3 and was increased in the absence of Trpc1. After co-expression of Trpc1 and Trpc3 in HEK-293 cells both proteins co-immunoprecipitate and form functional heteromeric channels, with TRPC1 reducing TRPC3 activity. In vitro, lack of Trpc3 reduced astrocyte proliferation and migration whereas the TRPC3 gain-of-function moonwalker mutation and Trpc1 deficiency increased astrocyte migration. In vivo, astrogliosis and cortex edema following stab wound injury were reduced in Trpc3-/- but increased in Trpc1-/- mice. In summary, our results show a decisive contribution of TRPC3 to astrocyte Ca2+ signaling, which is even augmented in the absence of Trpc1, in particular following brain injury. Targeted therapies to reduce TRPC3 channel activity in astrocytes might therefore be beneficial in traumatic brain injury.Wiley2017info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/87241098-113610.1002/glia.2318028636132Belkacemi T, Niermann A, Hofmann L, et al. TRPC1‐ and TRPC3‐dependent Ca2+ signaling in mouse cortical astrocytes affects injury‐evoked astrogliosis in vivo [en línea]. Glia. 2017;65(9):1535-1549. doi:10.1002/glia.23180 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/8724Glia. 2017;65(9):1535-1549reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:56:54Zoai:ucacris:123456789/8724instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:56:55.12Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse |
dc.title.none.fl_str_mv |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo |
title |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo |
spellingShingle |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo Belkacemi, Thabet TEJIDO NERVIOSO CELULAS CEREBRO CORTEZA CEREBRAL HERIDAS |
title_short |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo |
title_full |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo |
title_fullStr |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo |
title_full_unstemmed |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo |
title_sort |
TRPC1- and TRPC3-dependent Ca2+ signaling in mouse cortical astrocytes affects injury-evoked astrogliosis in vivo |
dc.creator.none.fl_str_mv |
Belkacemi, Thabet Niermann, Alexander Hofmann, Laura Wissenbach, Ulrich Birnbaumer, Lutz Leidinger, Petra Backes, Christina Meese, Eckart Keller, Andreas Bai, Xianshu Scheller, Anja Kirchhoff, Frank Philipp, Stephan E. Weissgerber, Petra Flockerzi, Veit Beck, Andreas |
author |
Belkacemi, Thabet |
author_facet |
Belkacemi, Thabet Niermann, Alexander Hofmann, Laura Wissenbach, Ulrich Birnbaumer, Lutz Leidinger, Petra Backes, Christina Meese, Eckart Keller, Andreas Bai, Xianshu Scheller, Anja Kirchhoff, Frank Philipp, Stephan E. Weissgerber, Petra Flockerzi, Veit Beck, Andreas |
author_role |
author |
author2 |
Niermann, Alexander Hofmann, Laura Wissenbach, Ulrich Birnbaumer, Lutz Leidinger, Petra Backes, Christina Meese, Eckart Keller, Andreas Bai, Xianshu Scheller, Anja Kirchhoff, Frank Philipp, Stephan E. Weissgerber, Petra Flockerzi, Veit Beck, Andreas |
author2_role |
author author author author author author author author author author author author author author author |
dc.subject.none.fl_str_mv |
TEJIDO NERVIOSO CELULAS CEREBRO CORTEZA CEREBRAL HERIDAS |
topic |
TEJIDO NERVIOSO CELULAS CEREBRO CORTEZA CEREBRAL HERIDAS |
dc.description.none.fl_txt_mv |
Fil: Belkacemi, Thabet. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania Fil: Niermann, Alexander. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania Fil: Hofmann, Laura. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania Fil: Wissenbach, Ulrich. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania Fil: Birnbaumer, Lutz. National Institute of Environmental Health Sciences. Research Triangle Park. Neurobiology Laboratory; Estados Unidos Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina Fil: Leidinger, Petra. Universität des Saarlandes. Institut für Humangenetik; Alemania Fil: Backes, Christina. Universität des Saarlandes. Klinische Bioinformatik; Alemania Fil: Meese, Eckart. Universität des Saarlandes. Institut für Humangenetik; Alemania Fil: Keller, Andreas. Universität des Saarlandes. Klinische Bioinformatik; Alemania Fil: Bai, Xianshu. Universität des Saarlandes. Molekulare Physiologie; Alemania Fil: Scheller, Anja. Universität des Saarlandes. Molekulare Physiologie; Alemania Fil: Kirchhoff, Frank. Universität des Saarlandes. Molekulare Physiologie; Alemania Fil: Philipp, Stephan E. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania Fil: Weissgerber, Petra. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania Fil: Flockerzi, Veit. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania Fil: Beck, Andreas. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania Fil: Beck, Andreas. Universität des Saarlandes. Molekulare Physiologie; Alemania Abstract: Following brain injury astrocytes change into a reactive state, proliferate and grow into the site of lesion, a process called astrogliosis, initiated and regulated by changes in cytoplasmic Ca2+ . Transient receptor potential canonical (TRPC) channels may contribute to Ca2+ influx but their presence and possible function in astrocytes is not known. By RT-PCR and RNA sequencing we identified transcripts of Trpc1, Trpc2, Trpc3, and Trpc4 in FACS-sorted glutamate aspartate transporter (GLAST)-positive cultured mouse cortical astrocytes and subcloned full-length Trpc1 and Trpc3 cDNAs from these cells. Ca2+ entry in cortical astrocytes depended on TRPC3 and was increased in the absence of Trpc1. After co-expression of Trpc1 and Trpc3 in HEK-293 cells both proteins co-immunoprecipitate and form functional heteromeric channels, with TRPC1 reducing TRPC3 activity. In vitro, lack of Trpc3 reduced astrocyte proliferation and migration whereas the TRPC3 gain-of-function moonwalker mutation and Trpc1 deficiency increased astrocyte migration. In vivo, astrogliosis and cortex edema following stab wound injury were reduced in Trpc3-/- but increased in Trpc1-/- mice. In summary, our results show a decisive contribution of TRPC3 to astrocyte Ca2+ signaling, which is even augmented in the absence of Trpc1, in particular following brain injury. Targeted therapies to reduce TRPC3 channel activity in astrocytes might therefore be beneficial in traumatic brain injury. |
description |
Fil: Belkacemi, Thabet. Universität des Saarlandes. Experimentelle und Klinische Pharmakologie und Toxikologie; Alemania |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
acceptedVersion |
dc.identifier.none.fl_str_mv |
https://repositorio.uca.edu.ar/handle/123456789/8724 1098-1136 10.1002/glia.23180 28636132 Belkacemi T, Niermann A, Hofmann L, et al. TRPC1‐ and TRPC3‐dependent Ca2+ signaling in mouse cortical astrocytes affects injury‐evoked astrogliosis in vivo [en línea]. Glia. 2017;65(9):1535-1549. doi:10.1002/glia.23180 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/8724 |
url |
https://repositorio.uca.edu.ar/handle/123456789/8724 |
identifier_str_mv |
1098-1136 10.1002/glia.23180 28636132 Belkacemi T, Niermann A, Hofmann L, et al. TRPC1‐ and TRPC3‐dependent Ca2+ signaling in mouse cortical astrocytes affects injury‐evoked astrogliosis in vivo [en línea]. Glia. 2017;65(9):1535-1549. doi:10.1002/glia.23180 Disponible en: https://repositorio.uca.edu.ar/handle/123456789/8724 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess https://creativecommons.org/licenses/by-nc-sa/4.0/ |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0/ |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
dc.source.none.fl_str_mv |
Glia. 2017;65(9):1535-1549 reponame:Repositorio Institucional (UCA) instname:Pontificia Universidad Católica Argentina |
reponame_str |
Repositorio Institucional (UCA) |
collection |
Repositorio Institucional (UCA) |
instname_str |
Pontificia Universidad Católica Argentina |
repository.name.fl_str_mv |
Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina |
repository.mail.fl_str_mv |
claudia_fernandez@uca.edu.ar |
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1836638347566514176 |
score |
13.13397 |