Effect of cadmium on 24-hour pattern in expression of redox enzime and clock genes in rat medial basal hypothalamus

• Autores: Jiménez Ortega, Vanesa; Cardinali, Daniel Pedro; Fernández Mateos, María P.; Ríos Lugo, María J.; Scacchi, Pablo; Esquifino, Ana I.
• Año de publicación: 2010
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Biometals. 2010, 23 (2)
• Materia: RITMO CIRCADIANO; OXIDO NITRICO SINTASA; HEMO-OXIGENASA; SUPEROXIDO DISMUTASA; CATALASA; GLUTATION PEROXIDASA; GLUTATION REDUCTASA; CADMIO; HIPOTALAMO MEDIO; EXPRESION GENICA
• Institución: Pontificia Universidad Católica Argentina

Fil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Cardinali, Daniel P. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas; Departamento de Docencia e Investigación; Argentina
Fil: Fernández Mateos, María P. Universidad Complutense. Facultad de Medicina. Departamento de Biología Celular; España
Fil: Ríos Lugo, María J. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Scacchi Bernasconi, Pablo A. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Departamento de Docencia e Investigación; Argentina
Fil: Esquifino, Ana I. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Abstract: The effect of cadmium (Cd) in the brain has been attributed to an increase in reactive oxygen species in cells, particularly when high amounts of the metal are given. In this study we examined the effect of a low dose of Cd (7.5 μg/day) on 24-h changes in expression of redox pathway enzyme and circadian genes in rat medial basal hypothalamus (MBH). Rats receiving CdCl2 (5 ppm in drinking water) or tap water for one month were killed at 6 different time intervals throughout a 24 h cycle. MBH mRNA levels were measured by real-time PCR analysis. In CdCl2 treated rats a disruption of 24-h pattern of hypothalamic gene expression of nitric oxide synthase (NOS)-1 and -2, heme oxygenase (HO)-1 and -2, Mn- superoxide dismutase (SOD), catalase, glutathione peroxidase and glutathione reductase was detectable. Mean levels of MBH mRNA for HO-2, Mn-SOD and catalase augmented after Cd intake, whereas those of NOS-2 decreased. After CdCl2 intake rats the 24-h pattern of clock gene expression in MBH seen in controls was significantly suppressed (Bmal1) or changed in phase (Per1, Per2, Cry2) while in the case of Clock significant 24-h variations were induced. The results are compatible with the view that a low amount of Cd given in tap water brought about significant changes in circadian expression of redox enzyme and clock genes in rat MBH.