Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state

Autores
Melchiorre, Mariana; Robert, German; Trippi, Victorio; Racca, Roberto Walter; Lascano, Hernan Ramiro
Año de publicación
2008
Idioma
alemán
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In previous works, we have established a correlation between antioxidant system response and tolerance to drought, osmotic stress and photooxidative stress of different wheat cultivars with contrasting drought tolerance. In the present work, a protocol to obtain and transform wheat protoplasts was established. Transgenic protoplasts with Manganese Superoxide Dismutase (Mn-SOD) (E.C.: 1.15.1.1) and Glutathione Reductase (GR) (E.C.: 1.6.4.2) overexpression in chloroplasts were obtained, and their responses to photooxidative stress were characterized. Protoplasts with Mn-SOD or GR overexpression, showed different responses and tolerance to photooxidative stress. Protoplasts with Mn-SOD overexpression showed lower levels of oxidative damage, higher level of endogenous hydrogen peroxide and a great induction of total SOD and GR activities during photooxidative treatments. In protoplasts with GR overexpression the oxidative damage provoked by the photooxidative treatment was similar to control protoplasts, the GSH content and GSH/GSH + GSSG ratio were higher than control and Mn-SOD transformed protoplast, and total SOD and GR activities were not induced. Our results suggest that the differential responses and tolerance to photooxidative stress given by Mn-SOD or GR overexpression, also depend on the effects of these enzyme activities over the cellular redox state balance, which modulate the responses to photooxidative stress.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Trippi, Victorio. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Trippi, Victorio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina Argentina
Fuente
Plant Growth Regulation 57 : 57-68 (2009)
Materia
Glutathione Reductase
Reactive Oxygen Species
Superoxide Dismutase
Glutatión Reductasa
Superóxido Dismutasa
Especies de Oxígeno Reactivo
Photooxidative Stress
Wheat Protoplasts
Estrés Fotooxidativo
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/10978
Acceder
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oai_identifier_str oai:localhost:20.500.12123/10978
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox stateMelchiorre, MarianaRobert, GermanTrippi, VictorioRacca, Roberto WalterLascano, Hernan RamiroGlutathione ReductaseReactive Oxygen SpeciesSuperoxide DismutaseGlutatión ReductasaSuperóxido DismutasaEspecies de Oxígeno ReactivoPhotooxidative StressWheat ProtoplastsEstrés FotooxidativoIn previous works, we have established a correlation between antioxidant system response and tolerance to drought, osmotic stress and photooxidative stress of different wheat cultivars with contrasting drought tolerance. In the present work, a protocol to obtain and transform wheat protoplasts was established. Transgenic protoplasts with Manganese Superoxide Dismutase (Mn-SOD) (E.C.: 1.15.1.1) and Glutathione Reductase (GR) (E.C.: 1.6.4.2) overexpression in chloroplasts were obtained, and their responses to photooxidative stress were characterized. Protoplasts with Mn-SOD or GR overexpression, showed different responses and tolerance to photooxidative stress. Protoplasts with Mn-SOD overexpression showed lower levels of oxidative damage, higher level of endogenous hydrogen peroxide and a great induction of total SOD and GR activities during photooxidative treatments. In protoplasts with GR overexpression the oxidative damage provoked by the photooxidative treatment was similar to control protoplasts, the GSH content and GSH/GSH + GSSG ratio were higher than control and Mn-SOD transformed protoplast, and total SOD and GR activities were not induced. Our results suggest that the differential responses and tolerance to photooxidative stress given by Mn-SOD or GR overexpression, also depend on the effects of these enzyme activities over the cellular redox state balance, which modulate the responses to photooxidative stress.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; ArgentinaFil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; ArgentinaFil: Trippi, Victorio. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Trippi, Victorio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; ArgentinaFil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; ArgentinaFil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); ArgentinaFil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina ArgentinaSpringer2021-12-23T10:54:41Z2021-12-23T10:54:41Z2008-08-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/10978https://link.springer.com/article/10.1007/s10725-008-9322-30167-69031573-5087 (online)https://doi.org/10.1007/s10725-008-9322-3Plant Growth Regulation 57 : 57-68 (2009)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariadeuinfo:eu-repo/semantics/restrictedAccess2025-10-16T09:30:20Zoai:localhost:20.500.12123/10978instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:30:20.925INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state
title Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state
spellingShingle Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state
Melchiorre, Mariana
Glutathione Reductase
Reactive Oxygen Species
Superoxide Dismutase
Glutatión Reductasa
Superóxido Dismutasa
Especies de Oxígeno Reactivo
Photooxidative Stress
Wheat Protoplasts
Estrés Fotooxidativo
title_short Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state
title_full Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state
title_fullStr Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state
title_full_unstemmed Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state
title_sort Superoxide dismutase and glutathione reductase overexpression in wheat protoplast: photooxidative stress tolerance and changes in cellular redox state
dc.creator.none.fl_str_mv Melchiorre, Mariana
Robert, German
Trippi, Victorio
Racca, Roberto Walter
Lascano, Hernan Ramiro
author Melchiorre, Mariana
author_facet Melchiorre, Mariana
Robert, German
Trippi, Victorio
Racca, Roberto Walter
Lascano, Hernan Ramiro
author_role author
author2 Robert, German
Trippi, Victorio
Racca, Roberto Walter
Lascano, Hernan Ramiro
author2_role author
author
author
author
dc.subject.none.fl_str_mv Glutathione Reductase
Reactive Oxygen Species
Superoxide Dismutase
Glutatión Reductasa
Superóxido Dismutasa
Especies de Oxígeno Reactivo
Photooxidative Stress
Wheat Protoplasts
Estrés Fotooxidativo
topic Glutathione Reductase
Reactive Oxygen Species
Superoxide Dismutase
Glutatión Reductasa
Superóxido Dismutasa
Especies de Oxígeno Reactivo
Photooxidative Stress
Wheat Protoplasts
Estrés Fotooxidativo
dc.description.none.fl_txt_mv In previous works, we have established a correlation between antioxidant system response and tolerance to drought, osmotic stress and photooxidative stress of different wheat cultivars with contrasting drought tolerance. In the present work, a protocol to obtain and transform wheat protoplasts was established. Transgenic protoplasts with Manganese Superoxide Dismutase (Mn-SOD) (E.C.: 1.15.1.1) and Glutathione Reductase (GR) (E.C.: 1.6.4.2) overexpression in chloroplasts were obtained, and their responses to photooxidative stress were characterized. Protoplasts with Mn-SOD or GR overexpression, showed different responses and tolerance to photooxidative stress. Protoplasts with Mn-SOD overexpression showed lower levels of oxidative damage, higher level of endogenous hydrogen peroxide and a great induction of total SOD and GR activities during photooxidative treatments. In protoplasts with GR overexpression the oxidative damage provoked by the photooxidative treatment was similar to control protoplasts, the GSH content and GSH/GSH + GSSG ratio were higher than control and Mn-SOD transformed protoplast, and total SOD and GR activities were not induced. Our results suggest that the differential responses and tolerance to photooxidative stress given by Mn-SOD or GR overexpression, also depend on the effects of these enzyme activities over the cellular redox state balance, which modulate the responses to photooxidative stress.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Melchiorre, Mariana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Trippi, Victorio. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Trippi, Victorio. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Racca, Roberto Walter. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina
Fil: Lascano, Hernán Ramiro. Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET); Argentina
Fil: Lascano, Hernán Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fitopatología y Fisiología Vegetal; Argentina Argentina
description In previous works, we have established a correlation between antioxidant system response and tolerance to drought, osmotic stress and photooxidative stress of different wheat cultivars with contrasting drought tolerance. In the present work, a protocol to obtain and transform wheat protoplasts was established. Transgenic protoplasts with Manganese Superoxide Dismutase (Mn-SOD) (E.C.: 1.15.1.1) and Glutathione Reductase (GR) (E.C.: 1.6.4.2) overexpression in chloroplasts were obtained, and their responses to photooxidative stress were characterized. Protoplasts with Mn-SOD or GR overexpression, showed different responses and tolerance to photooxidative stress. Protoplasts with Mn-SOD overexpression showed lower levels of oxidative damage, higher level of endogenous hydrogen peroxide and a great induction of total SOD and GR activities during photooxidative treatments. In protoplasts with GR overexpression the oxidative damage provoked by the photooxidative treatment was similar to control protoplasts, the GSH content and GSH/GSH + GSSG ratio were higher than control and Mn-SOD transformed protoplast, and total SOD and GR activities were not induced. Our results suggest that the differential responses and tolerance to photooxidative stress given by Mn-SOD or GR overexpression, also depend on the effects of these enzyme activities over the cellular redox state balance, which modulate the responses to photooxidative stress.
publishDate 2008
dc.date.none.fl_str_mv 2008-08-26
2021-12-23T10:54:41Z
2021-12-23T10:54:41Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/10978
https://link.springer.com/article/10.1007/s10725-008-9322-3
0167-6903
1573-5087 (online)
https://doi.org/10.1007/s10725-008-9322-3
url http://hdl.handle.net/20.500.12123/10978
https://link.springer.com/article/10.1007/s10725-008-9322-3
https://doi.org/10.1007/s10725-008-9322-3
identifier_str_mv 0167-6903
1573-5087 (online)
dc.language.none.fl_str_mv deu
language deu
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Plant Growth Regulation 57 : 57-68 (2009)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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