24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment

Autores
Jiménez Ortega, Vanesa; Cano Barquilla, Pilar; Cardinali, Daniel Pedro; Esquifino, Ana I.
Año de publicación
2009
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Cano Barquilla, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Cardinali, Daniel P. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología; Argentina
Fil: Esquifino, Ana I. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Abstract: The 24-h changes in medial basal hypothalamic (MBH) gene expression of redox pathway enzymes nitric oxide synthase (NOS)-1 and NOS-2, heme oxygenase (HO)-1 and HO-2, Cu/Znand Mn-superoxide dismutases (SOD) and catalase were examined in adult male Wistar rats. Half of the animals received melatonin (ca. 60 μg/day) in the drinking water. After 1 month, rats were killed at six different time intervals, throughout a 24-h cycle. MBH mRNA levels were measured by real-time PCR analysis. In controls NOS-1 mRNA expression did not exhibit 24-h variations while NOS-2 gene expression peaked at the early light phase of cycle. MBH mRNA for both the inducible (HO-1) and constitutive (HO-2) isoforms of HO showed a significant 24 h pattern with a maximum at the middle of the dark phase or at the early light phase, respectively. None of mRNAs encoding Cu/Zn-SOD, Mn-SOD and catalase exhibited significant 24-h variations in rat MBH under control conditions. Chronic melatonin administration in the drinking water decreased significantly mRNAs for NOS-1, NOS-2, HO-1 and HO-2 as well as changed their 24-h profile. Melatonin augmented gene expression of the antioxidant enzymes Cu/Zn-SOD, Mn-SOD or catalase at certain time intervals only
Fuente
Redox Report. 2009, 14 (3)
Materia
MELATONINA
RITMO CIRCADIANO
HIPOTALAMO MEDIO
EXPRESION DE GENES
CATALASA
OXIDO NITRICO SINTASA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
Repositorio Institucional (UCA)
Institución
Pontificia Universidad Católica Argentina
OAI Identificador
oai:ucacris:123456789/1626
Acceder
id RIUCA_0b9e13209837b7046e9628e0f6f8bc4e
oai_identifier_str oai:ucacris:123456789/1626
network_acronym_str RIUCA
repository_id_str 2585
network_name_str Repositorio Institucional (UCA)
spelling 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatmentJiménez Ortega, VanesaCano Barquilla, PilarCardinali, Daniel PedroEsquifino, Ana I.MELATONINARITMO CIRCADIANOHIPOTALAMO MEDIOEXPRESION DE GENESCATALASAOXIDO NITRICO SINTASAFil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; EspañaFil: Cano Barquilla, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; EspañaFil: Cardinali, Daniel P. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología; ArgentinaFil: Esquifino, Ana I. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; EspañaAbstract: The 24-h changes in medial basal hypothalamic (MBH) gene expression of redox pathway enzymes nitric oxide synthase (NOS)-1 and NOS-2, heme oxygenase (HO)-1 and HO-2, Cu/Znand Mn-superoxide dismutases (SOD) and catalase were examined in adult male Wistar rats. Half of the animals received melatonin (ca. 60 μg/day) in the drinking water. After 1 month, rats were killed at six different time intervals, throughout a 24-h cycle. MBH mRNA levels were measured by real-time PCR analysis. In controls NOS-1 mRNA expression did not exhibit 24-h variations while NOS-2 gene expression peaked at the early light phase of cycle. MBH mRNA for both the inducible (HO-1) and constitutive (HO-2) isoforms of HO showed a significant 24 h pattern with a maximum at the middle of the dark phase or at the early light phase, respectively. None of mRNAs encoding Cu/Zn-SOD, Mn-SOD and catalase exhibited significant 24-h variations in rat MBH under control conditions. Chronic melatonin administration in the drinking water decreased significantly mRNAs for NOS-1, NOS-2, HO-1 and HO-2 as well as changed their 24-h profile. Melatonin augmented gene expression of the antioxidant enzymes Cu/Zn-SOD, Mn-SOD or catalase at certain time intervals onlyTaylor & Francis2009info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://repositorio.uca.edu.ar/handle/123456789/16261351-0002 (impreso)1743-2928 (online)10.1179/135100009X392548Jiménez-Ortega, V., et al. 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment [en línea]. Redox Report 2009, 14 (3). doi:10.1179/135100009X392548. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1626Redox Report. 2009, 14 (3)reponame:Repositorio Institucional (UCA)instname:Pontificia Universidad Católica Argentinaengenginfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/4.0/2025-07-03T10:55:19Zoai:ucacris:123456789/1626instacron:UCAInstitucionalhttps://repositorio.uca.edu.ar/Universidad privadaNo correspondehttps://repositorio.uca.edu.ar/oaiclaudia_fernandez@uca.edu.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:25852025-07-03 10:55:20.008Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentinafalse
dc.title.none.fl_str_mv 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment
title 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment
spellingShingle 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment
Jiménez Ortega, Vanesa
MELATONINA
RITMO CIRCADIANO
HIPOTALAMO MEDIO
EXPRESION DE GENES
CATALASA
OXIDO NITRICO SINTASA
title_short 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment
title_full 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment
title_fullStr 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment
title_full_unstemmed 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment
title_sort 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment
dc.creator.none.fl_str_mv Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Esquifino, Ana I.
author Jiménez Ortega, Vanesa
author_facet Jiménez Ortega, Vanesa
Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Esquifino, Ana I.
author_role author
author2 Cano Barquilla, Pilar
Cardinali, Daniel Pedro
Esquifino, Ana I.
author2_role author
author
author
dc.subject.none.fl_str_mv MELATONINA
RITMO CIRCADIANO
HIPOTALAMO MEDIO
EXPRESION DE GENES
CATALASA
OXIDO NITRICO SINTASA
topic MELATONINA
RITMO CIRCADIANO
HIPOTALAMO MEDIO
EXPRESION DE GENES
CATALASA
OXIDO NITRICO SINTASA
dc.description.none.fl_txt_mv Fil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Cano Barquilla, Pilar. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Fil: Cardinali, Daniel P. Universidad de Buenos Aires. Facultad de Medicina. Departamento de Fisiología; Argentina
Fil: Esquifino, Ana I. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
Abstract: The 24-h changes in medial basal hypothalamic (MBH) gene expression of redox pathway enzymes nitric oxide synthase (NOS)-1 and NOS-2, heme oxygenase (HO)-1 and HO-2, Cu/Znand Mn-superoxide dismutases (SOD) and catalase were examined in adult male Wistar rats. Half of the animals received melatonin (ca. 60 μg/day) in the drinking water. After 1 month, rats were killed at six different time intervals, throughout a 24-h cycle. MBH mRNA levels were measured by real-time PCR analysis. In controls NOS-1 mRNA expression did not exhibit 24-h variations while NOS-2 gene expression peaked at the early light phase of cycle. MBH mRNA for both the inducible (HO-1) and constitutive (HO-2) isoforms of HO showed a significant 24 h pattern with a maximum at the middle of the dark phase or at the early light phase, respectively. None of mRNAs encoding Cu/Zn-SOD, Mn-SOD and catalase exhibited significant 24-h variations in rat MBH under control conditions. Chronic melatonin administration in the drinking water decreased significantly mRNAs for NOS-1, NOS-2, HO-1 and HO-2 as well as changed their 24-h profile. Melatonin augmented gene expression of the antioxidant enzymes Cu/Zn-SOD, Mn-SOD or catalase at certain time intervals only
description Fil: Jiménez Ortega, Vanesa. Universidad Complutense. Facultad de Medicina. Departamento de Bioquímica y Biología Molecular III; España
publishDate 2009
dc.date.none.fl_str_mv 2009
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://repositorio.uca.edu.ar/handle/123456789/1626
1351-0002 (impreso)
1743-2928 (online)
10.1179/135100009X392548
Jiménez-Ortega, V., et al. 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment [en línea]. Redox Report 2009, 14 (3). doi:10.1179/135100009X392548. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1626
url https://repositorio.uca.edu.ar/handle/123456789/1626
identifier_str_mv 1351-0002 (impreso)
1743-2928 (online)
10.1179/135100009X392548
Jiménez-Ortega, V., et al. 24-hour rhythm in gene expression of redox pathway enzymes in rat hypothalamus : effect of melatonin treatment [en línea]. Redox Report 2009, 14 (3). doi:10.1179/135100009X392548. Disponible en: https://repositorio.uca.edu.ar/handle/123456789/1626
dc.language.none.fl_str_mv eng
eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/4.0/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor & Francis
publisher.none.fl_str_mv Taylor & Francis
dc.source.none.fl_str_mv Redox Report. 2009, 14 (3)
reponame:Repositorio Institucional (UCA)
instname:Pontificia Universidad Católica Argentina
reponame_str Repositorio Institucional (UCA)
collection Repositorio Institucional (UCA)
instname_str Pontificia Universidad Católica Argentina
repository.name.fl_str_mv Repositorio Institucional (UCA) - Pontificia Universidad Católica Argentina
repository.mail.fl_str_mv claudia_fernandez@uca.edu.ar
_version_ 1836638330564902912
score 13.070432

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