Major contribution of the 3/6/7 class of TRPC channels to myocardial ischemia/reperfusion and cellular hypoxia/reoxygenation injuries

• Autores: He, Xiju; Li, Shoutian; Liu, Benju; Susperreguy, Sebastián; Formoso, Karina; Yao, Jinghong; Kang, Jinsong; Anbing, Shi; Birnbaumer, Lutz; Liao, Yanhong
• Año de publicación: 2017
• Idioma: inglés
• Tipo de recurso: artículo
• Fuente: Proceedings of the National Academy of Sciences 2017, 114 (23); ISSN 0027-8424 (impreso); ISSN 1091-6490 (online)
• Materia: INFARTO DEL MIOCARDIO; CALCIO; APOPTOSIS; MUERTE CELULAR
• Institución: Pontificia Universidad Católica Argentina

Fil: He, Xiju. Huazhong University of Science and Technology. Tongji Medical College. Department of Anatomy; China
Fil: He, Xiju. Huazhong University of Science and Technology. Tongji Medical College. Institute of Brain Research; China
Fil: He, Xiju. Hubei University of Medicine. Department of Anatomy; China
Fil: Li, Shoutian. Huazhong University of Science and Technology. Tongji Medical College. Department of Anatomy; China
Fil: Li, Shoutian. Huazhong University of Science and Technology. Tongji Medical College. Institute of Brain Research; China
Fil: Liu, Benju. Huazhong University of Science and Technology. Tongji Medical College. Department of Anatomy; China
Fil: Liu, Benju. Huazhong University of Science and Technology. Tongji Medical College. Institute of Brain Research; China
Fil: Susperreguy, Sebastian. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Formoso, Karina. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Yao, Jinghong. Huazhong University of Science and Technology. Tongji Medical College. Union Hospital. Department of Infectious Disease; China
Fil: Kang, Jinsong. Huazhong University of Science and Technology. Tongji Medical College. Tongji Hospital. Department of Surgery; China
Fil: Anbing, Shi. Huazhong University of Science and Technology. Tongji Medical College. Department of Biochemistry and Molecular Biology; China
Fil: Birnbaumer, Lutz. Pontificia Universidad Católica Argentina. Facultad de Ciencias Médicas. Instituto de Investigaciones Biomédicas; Argentina
Fil: Birnbaumer, Lutz. Research Triangle Park. National Institute of Environmental Health Sciences. Neurobiology Laboratory; Estados Unidos
Fil: Liao, Yanhong. Huazhong University of Science and Technology. Tongji Medical College. Department of Anatomy; China
Fil: Liao, Yanhong. Huazhong University of Science and Technology. Tongji Medical College. Institute of Brain Research; China
Abstract: The injury phase after myocardial infarcts occurs during reperfusion and is a consequence of calcium release from internal stores combined with calcium entry, leading to cell death by apoptopic and necrotic processes. The mechanism(s) by which calcium enters cells has(ve) not been identified. Here, we identify canonical transient receptor potential channels (TRPC) 3 and 6 as the cation channels through which most of the damaging calcium enters cells to trigger their death, and we describe mechanisms activated during the injury phase. Working in vitro with H9c2 cardiomyoblasts subjected to 9-h hypoxia followed by 6-h reoxygenation (H/R), and analyzing changes occurring in areas-at-risk (AARs) of murine hearts subjected to a 30-min ischemia followed by 24-h reperfusion (I/R) protocol, we found: (i) that blocking TRPCwith SKF96365 significantly ameliorated damage induced by H/R, including development of the mitochondrial permeability transition and proapoptotic changes in Bcl2/BAX ratios; and (ii) that AAR tissues had increased TUNEL+ cells, augmented Bcl2/BAX ratios, and increased p(S240)NFATc3, p(S473) AKT, p(S9)GSK3β, and TRPC3 and -6 proteins, consistent with activation of a positive-feedback loop in which calcium entering through TRPCs activates calcineurin-mediated NFATc3-directed transcription of TRPC genes, leading to more Ca2+ entry. All these changes were markedly reduced in mice lacking TRPC3, -6, and -7. The changes caused by I/R in AAR tissues were matched by those seen after H/R in cardiomyoblasts in all aspects except for p-AKT and p-GSK3β, which were decreased after H/R in cardiomyoblasts instead of increased. TRPC should be promising targets for pharmacologic intervention after cardiac infarcts.