An Experimental Model of Myocardial Infarction for Studying Cardiac Repair and Remodeling in Knockout Mice

Autores
Fontana Estevez, Florencia Sofía; Miksztowicz, Verónica; Seropian, Ignacio Miguel; Cassaglia, Pablo; Bustos, Romina; Touceda, Vanessa Michelle; Cianciulli, Tomás; Cassanova, Verónica; Morales, Celina; González, Germán Esteban
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Cardiovascular disease is the most prevalent cause of death in Western countries, with acute myocardial infarction (MI) being the most prevalent form. This paper describes a protocol for studying the role of galectin 3 (Gal-3) in the temporal evolution of cardiac healing and remodeling in an experimental animal model of MI. The procedures described include an experimental model of MI with a permanent coronary ligature in male C57BL/6J (control) and Gal-3 knockout (KO) mice, an echocardiography procedure to study cardiac remodeling and systolic function in vivo, a histological evaluation of interstitial myocardial fibrosis with picrosirius red-stained and rhodamine-conjugated lectin-stained sections for studying myocyte hypertrophy by the cross-sectional area (MCSA), and the quantification of infarct size and cardiac remodeling (scar thinning, septum thickness, and expansion index) by planimetry in slices stained with Masson´s trichrome and triphenyl tetrazolium chloride. Gal-3 KO mice with MI showed disrupted cardiac remodeling and an increase in the scar thinning ratio and the expansion index. At the onset of MI, myocardial function and cardiac remodeling were also severely affected. At 4 weeks post MI, the natural evolution of fibrosis in infarcted Gal-3 KO mice was also affected. In summary, the experimental model of MI is a suitable model for studying the temporal evolution of cardiac repair and remodeling in mice with the genetic deletion of Gal-3 and other animal models. The lack of Gal-3 affects the dynamics of cardiac repair and disrupts the evolution of cardiac remodeling and function after MI.
Fil: Fontana Estevez, Florencia Sofía. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina
Fil: Miksztowicz, Verónica. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina
Fil: Seropian, Ignacio Miguel. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Hospital Italiano. Instituto Universitario. Escuela de Medicina; Argentina
Fil: Cassaglia, Pablo. Universidad de Buenos Aires; Argentina
Fil: Bustos, Romina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina
Fil: Touceda, Vanessa Michelle. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cianciulli, Tomás. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Cosme Argerich; Argentina
Fil: Cassanova, Verónica. Universidad de Buenos Aires; Argentina
Fil: Morales, Celina. Universidad de Buenos Aires; Argentina
Fil: González, Germán Esteban. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Materia
GALECTINA 3
INFARTO DE MIOCARDIO
REMODELADO POST INFARTO
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/227990
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/227990
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An Experimental Model of Myocardial Infarction for Studying Cardiac Repair and Remodeling in Knockout MiceFontana Estevez, Florencia SofíaMiksztowicz, VerónicaSeropian, Ignacio MiguelCassaglia, PabloBustos, RominaTouceda, Vanessa MichelleCianciulli, TomásCassanova, VerónicaMorales, CelinaGonzález, Germán EstebanGALECTINA 3INFARTO DE MIOCARDIOREMODELADO POST INFARTOhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Cardiovascular disease is the most prevalent cause of death in Western countries, with acute myocardial infarction (MI) being the most prevalent form. This paper describes a protocol for studying the role of galectin 3 (Gal-3) in the temporal evolution of cardiac healing and remodeling in an experimental animal model of MI. The procedures described include an experimental model of MI with a permanent coronary ligature in male C57BL/6J (control) and Gal-3 knockout (KO) mice, an echocardiography procedure to study cardiac remodeling and systolic function in vivo, a histological evaluation of interstitial myocardial fibrosis with picrosirius red-stained and rhodamine-conjugated lectin-stained sections for studying myocyte hypertrophy by the cross-sectional area (MCSA), and the quantification of infarct size and cardiac remodeling (scar thinning, septum thickness, and expansion index) by planimetry in slices stained with Masson´s trichrome and triphenyl tetrazolium chloride. Gal-3 KO mice with MI showed disrupted cardiac remodeling and an increase in the scar thinning ratio and the expansion index. At the onset of MI, myocardial function and cardiac remodeling were also severely affected. At 4 weeks post MI, the natural evolution of fibrosis in infarcted Gal-3 KO mice was also affected. In summary, the experimental model of MI is a suitable model for studying the temporal evolution of cardiac repair and remodeling in mice with the genetic deletion of Gal-3 and other animal models. The lack of Gal-3 affects the dynamics of cardiac repair and disrupts the evolution of cardiac remodeling and function after MI.Fil: Fontana Estevez, Florencia Sofía. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; ArgentinaFil: Miksztowicz, Verónica. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; ArgentinaFil: Seropian, Ignacio Miguel. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Hospital Italiano. Instituto Universitario. Escuela de Medicina; ArgentinaFil: Cassaglia, Pablo. Universidad de Buenos Aires; ArgentinaFil: Bustos, Romina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; ArgentinaFil: Touceda, Vanessa Michelle. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Cianciulli, Tomás. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Cosme Argerich; ArgentinaFil: Cassanova, Verónica. Universidad de Buenos Aires; ArgentinaFil: Morales, Celina. Universidad de Buenos Aires; ArgentinaFil: González, Germán Esteban. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaJournal of Visualized Experiments2023-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/227990Fontana Estevez, Florencia Sofía; Miksztowicz, Verónica; Seropian, Ignacio Miguel; Cassaglia, Pablo; Bustos, Romina; et al.; An Experimental Model of Myocardial Infarction for Studying Cardiac Repair and Remodeling in Knockout Mice; Journal of Visualized Experiments; Journal of Visualized Experiments; 2023; 197; 7-2023; 1-151940-087XCONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.3791/64143info:eu-repo/semantics/altIdentifier/url/https://www.jove.com/es/t/64143/an-experimental-model-myocardial-infarction-for-studying-cardiacinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T10:00:59Zoai:ri.conicet.gov.ar:11336/227990instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 10:00:59.846CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An Experimental Model of Myocardial Infarction for Studying Cardiac Repair and Remodeling in Knockout Mice
title An Experimental Model of Myocardial Infarction for Studying Cardiac Repair and Remodeling in Knockout Mice
spellingShingle An Experimental Model of Myocardial Infarction for Studying Cardiac Repair and Remodeling in Knockout Mice
Fontana Estevez, Florencia Sofía
GALECTINA 3
INFARTO DE MIOCARDIO
REMODELADO POST INFARTO
title_short An Experimental Model of Myocardial Infarction for Studying Cardiac Repair and Remodeling in Knockout Mice
title_full An Experimental Model of Myocardial Infarction for Studying Cardiac Repair and Remodeling in Knockout Mice
title_fullStr An Experimental Model of Myocardial Infarction for Studying Cardiac Repair and Remodeling in Knockout Mice
title_full_unstemmed An Experimental Model of Myocardial Infarction for Studying Cardiac Repair and Remodeling in Knockout Mice
title_sort An Experimental Model of Myocardial Infarction for Studying Cardiac Repair and Remodeling in Knockout Mice
dc.creator.none.fl_str_mv Fontana Estevez, Florencia Sofía
Miksztowicz, Verónica
Seropian, Ignacio Miguel
Cassaglia, Pablo
Bustos, Romina
Touceda, Vanessa Michelle
Cianciulli, Tomás
Cassanova, Verónica
Morales, Celina
González, Germán Esteban
author Fontana Estevez, Florencia Sofía
author_facet Fontana Estevez, Florencia Sofía
Miksztowicz, Verónica
Seropian, Ignacio Miguel
Cassaglia, Pablo
Bustos, Romina
Touceda, Vanessa Michelle
Cianciulli, Tomás
Cassanova, Verónica
Morales, Celina
González, Germán Esteban
author_role author
author2 Miksztowicz, Verónica
Seropian, Ignacio Miguel
Cassaglia, Pablo
Bustos, Romina
Touceda, Vanessa Michelle
Cianciulli, Tomás
Cassanova, Verónica
Morales, Celina
González, Germán Esteban
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv GALECTINA 3
INFARTO DE MIOCARDIO
REMODELADO POST INFARTO
topic GALECTINA 3
INFARTO DE MIOCARDIO
REMODELADO POST INFARTO
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Cardiovascular disease is the most prevalent cause of death in Western countries, with acute myocardial infarction (MI) being the most prevalent form. This paper describes a protocol for studying the role of galectin 3 (Gal-3) in the temporal evolution of cardiac healing and remodeling in an experimental animal model of MI. The procedures described include an experimental model of MI with a permanent coronary ligature in male C57BL/6J (control) and Gal-3 knockout (KO) mice, an echocardiography procedure to study cardiac remodeling and systolic function in vivo, a histological evaluation of interstitial myocardial fibrosis with picrosirius red-stained and rhodamine-conjugated lectin-stained sections for studying myocyte hypertrophy by the cross-sectional area (MCSA), and the quantification of infarct size and cardiac remodeling (scar thinning, septum thickness, and expansion index) by planimetry in slices stained with Masson´s trichrome and triphenyl tetrazolium chloride. Gal-3 KO mice with MI showed disrupted cardiac remodeling and an increase in the scar thinning ratio and the expansion index. At the onset of MI, myocardial function and cardiac remodeling were also severely affected. At 4 weeks post MI, the natural evolution of fibrosis in infarcted Gal-3 KO mice was also affected. In summary, the experimental model of MI is a suitable model for studying the temporal evolution of cardiac repair and remodeling in mice with the genetic deletion of Gal-3 and other animal models. The lack of Gal-3 affects the dynamics of cardiac repair and disrupts the evolution of cardiac remodeling and function after MI.
Fil: Fontana Estevez, Florencia Sofía. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina
Fil: Miksztowicz, Verónica. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina
Fil: Seropian, Ignacio Miguel. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Hospital Italiano. Instituto Universitario. Escuela de Medicina; Argentina
Fil: Cassaglia, Pablo. Universidad de Buenos Aires; Argentina
Fil: Bustos, Romina. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina
Fil: Touceda, Vanessa Michelle. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Cianciulli, Tomás. Gobierno de la Ciudad Autónoma de Buenos Aires. Hospital General de Agudos Doctor Cosme Argerich; Argentina
Fil: Cassanova, Verónica. Universidad de Buenos Aires; Argentina
Fil: Morales, Celina. Universidad de Buenos Aires; Argentina
Fil: González, Germán Esteban. Pontificia Universidad Católica Argentina "Santa María de los Buenos Aires"; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
description Cardiovascular disease is the most prevalent cause of death in Western countries, with acute myocardial infarction (MI) being the most prevalent form. This paper describes a protocol for studying the role of galectin 3 (Gal-3) in the temporal evolution of cardiac healing and remodeling in an experimental animal model of MI. The procedures described include an experimental model of MI with a permanent coronary ligature in male C57BL/6J (control) and Gal-3 knockout (KO) mice, an echocardiography procedure to study cardiac remodeling and systolic function in vivo, a histological evaluation of interstitial myocardial fibrosis with picrosirius red-stained and rhodamine-conjugated lectin-stained sections for studying myocyte hypertrophy by the cross-sectional area (MCSA), and the quantification of infarct size and cardiac remodeling (scar thinning, septum thickness, and expansion index) by planimetry in slices stained with Masson´s trichrome and triphenyl tetrazolium chloride. Gal-3 KO mice with MI showed disrupted cardiac remodeling and an increase in the scar thinning ratio and the expansion index. At the onset of MI, myocardial function and cardiac remodeling were also severely affected. At 4 weeks post MI, the natural evolution of fibrosis in infarcted Gal-3 KO mice was also affected. In summary, the experimental model of MI is a suitable model for studying the temporal evolution of cardiac repair and remodeling in mice with the genetic deletion of Gal-3 and other animal models. The lack of Gal-3 affects the dynamics of cardiac repair and disrupts the evolution of cardiac remodeling and function after MI.
publishDate 2023
dc.date.none.fl_str_mv 2023-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/227990
Fontana Estevez, Florencia Sofía; Miksztowicz, Verónica; Seropian, Ignacio Miguel; Cassaglia, Pablo; Bustos, Romina; et al.; An Experimental Model of Myocardial Infarction for Studying Cardiac Repair and Remodeling in Knockout Mice; Journal of Visualized Experiments; Journal of Visualized Experiments; 2023; 197; 7-2023; 1-15
1940-087X
CONICET Digital
CONICET
url http://hdl.handle.net/11336/227990
identifier_str_mv Fontana Estevez, Florencia Sofía; Miksztowicz, Verónica; Seropian, Ignacio Miguel; Cassaglia, Pablo; Bustos, Romina; et al.; An Experimental Model of Myocardial Infarction for Studying Cardiac Repair and Remodeling in Knockout Mice; Journal of Visualized Experiments; Journal of Visualized Experiments; 2023; 197; 7-2023; 1-15
1940-087X
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3791/64143
info:eu-repo/semantics/altIdentifier/url/https://www.jove.com/es/t/64143/an-experimental-model-myocardial-infarction-for-studying-cardiac
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Journal of Visualized Experiments
publisher.none.fl_str_mv Journal of Visualized Experiments
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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