Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function

Autores
Schmitt, Luisa O.; Blanco, Antonella; Lima, Sheila V.; Mancini, Gianni; Mendes, Natalia F.; Latini, Alexandra; Gaspar, Joana M.
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.
Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.
Fil: Blanco, Antonella. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica, Argentina.
Fil: Blanco, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina.
Fil: Blanco, Antonella. Federal University of Santa Catarina. School of Biological Sciences. Department of Biochemistry Laboratory of Bioenergetics and Oxidative Stress, Brazil.
Fil: Lima, Sheila V. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.
Fil: Mancini, Gianni. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.
Fil: Mendes, Natalia F. University of Campinas. School of Medical Sciences. Department of Translational Medicine (Section of Pharmacology), Brazil.
Fil: Latini, Alexandra. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.
Fil: Latini, Alexandra. Federal University of Santa Catarina. School of Biological Sciences. Department of Biochemistry Laboratory of Bioenergetics and Oxidative Stress, Brazil.
Fil: Gaspar, Joana M. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.
Fil: Gaspar, Joana M. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.
Consumption of high-fat diets (HFD) is associated with brain alterations, including changes in feeding behavior, cognitive decline, and dementia. Astrocytes play a role in HFD-induced neuroinflammation and brain dysfunction; however, this process is not entirely understood. We hypothesized that exposure to saturated fatty acids can compromise astrocyte viability and mitochondrial function. The C6 (astrocytes) cell line was treated with palmitate or stearate (200 μM and 400 μM) for 6 h. Cell viability, morphology, inflammatory markers, and oxidative stress were evaluated. To assess mitochondrial function, various parameters were measured (membrane potential, mass, respiration, and complex activities). We observed that 6 h of treatment with 400 μM palmitate decreased cell viability, and treatment with 200 μM palmitate changed the astrocyte morphology. Palmitate increased inflammatory markers (TNF-α and IL6) but did not induce oxidative stress. Palmitate significantly decreased the mitochondrial membrane potential and mitochondrial mass. Complex I activity also decreased in palmitate-treated cells; however, no changes were observed in mitochondrial respiration. In conclusion, palmitate, a saturated fatty acid, induces inflammation and impairs mitochondrial function, leading to reduced astrocytic cell viability and changes in cellular morphology. Our study provides valuable insights into the potential mechanisms underlying the relationship between saturated fatty acids, astrocytes, and mitochondrial function in obesity-related brain dysfunction.
info:eu-repo/semantics/publishedVersion
Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.
Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.
Fil: Blanco, Antonella. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica, Argentina.
Fil: Blanco, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina.
Fil: Blanco, Antonella. Federal University of Santa Catarina. School of Biological Sciences. Department of Biochemistry Laboratory of Bioenergetics and Oxidative Stress, Brazil.
Fil: Lima, Sheila V. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.
Fil: Mancini, Gianni. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.
Fil: Mendes, Natalia F. University of Campinas. School of Medical Sciences. Department of Translational Medicine (Section of Pharmacology), Brazil.
Fil: Latini, Alexandra. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.
Fil: Latini, Alexandra. Federal University of Santa Catarina. School of Biological Sciences. Department of Biochemistry Laboratory of Bioenergetics and Oxidative Stress, Brazil.
Fil: Gaspar, Joana M. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.
Fil: Gaspar, Joana M. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.
Materia
Obesity
Inflammation
Mitochondria
Saturated fatty acids
Astrocytes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Repositorio Digital Universitario (UNC)
Institución
Universidad Nacional de Córdoba
OAI Identificador
oai:rdu.unc.edu.ar:11086/552568
Acceder
id RDUUNC_9f9308d0926deaca91e021ebc796a14d
oai_identifier_str oai:rdu.unc.edu.ar:11086/552568
network_acronym_str RDUUNC
repository_id_str 2572
network_name_str Repositorio Digital Universitario (UNC)
spelling Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial FunctionSchmitt, Luisa O.Blanco, AntonellaLima, Sheila V.Mancini, GianniMendes, Natalia F.Latini, AlexandraGaspar, Joana M.ObesityInflammationMitochondriaSaturated fatty acidsAstrocytesFil: Schmitt, Luisa O. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.Fil: Blanco, Antonella. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica, Argentina.Fil: Blanco, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina.Fil: Blanco, Antonella. Federal University of Santa Catarina. School of Biological Sciences. Department of Biochemistry Laboratory of Bioenergetics and Oxidative Stress, Brazil.Fil: Lima, Sheila V. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.Fil: Mancini, Gianni. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.Fil: Mendes, Natalia F. University of Campinas. School of Medical Sciences. Department of Translational Medicine (Section of Pharmacology), Brazil.Fil: Latini, Alexandra. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.Fil: Latini, Alexandra. Federal University of Santa Catarina. School of Biological Sciences. Department of Biochemistry Laboratory of Bioenergetics and Oxidative Stress, Brazil.Fil: Gaspar, Joana M. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.Fil: Gaspar, Joana M. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.Consumption of high-fat diets (HFD) is associated with brain alterations, including changes in feeding behavior, cognitive decline, and dementia. Astrocytes play a role in HFD-induced neuroinflammation and brain dysfunction; however, this process is not entirely understood. We hypothesized that exposure to saturated fatty acids can compromise astrocyte viability and mitochondrial function. The C6 (astrocytes) cell line was treated with palmitate or stearate (200 μM and 400 μM) for 6 h. Cell viability, morphology, inflammatory markers, and oxidative stress were evaluated. To assess mitochondrial function, various parameters were measured (membrane potential, mass, respiration, and complex activities). We observed that 6 h of treatment with 400 μM palmitate decreased cell viability, and treatment with 200 μM palmitate changed the astrocyte morphology. Palmitate increased inflammatory markers (TNF-α and IL6) but did not induce oxidative stress. Palmitate significantly decreased the mitochondrial membrane potential and mitochondrial mass. Complex I activity also decreased in palmitate-treated cells; however, no changes were observed in mitochondrial respiration. In conclusion, palmitate, a saturated fatty acid, induces inflammation and impairs mitochondrial function, leading to reduced astrocytic cell viability and changes in cellular morphology. Our study provides valuable insights into the potential mechanisms underlying the relationship between saturated fatty acids, astrocytes, and mitochondrial function in obesity-related brain dysfunction.info:eu-repo/semantics/publishedVersionFil: Schmitt, Luisa O. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.Fil: Blanco, Antonella. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica, Argentina.Fil: Blanco, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina.Fil: Blanco, Antonella. Federal University of Santa Catarina. School of Biological Sciences. Department of Biochemistry Laboratory of Bioenergetics and Oxidative Stress, Brazil.Fil: Lima, Sheila V. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.Fil: Mancini, Gianni. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.Fil: Mendes, Natalia F. University of Campinas. School of Medical Sciences. Department of Translational Medicine (Section of Pharmacology), Brazil.Fil: Latini, Alexandra. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.Fil: Latini, Alexandra. Federal University of Santa Catarina. School of Biological Sciences. Department of Biochemistry Laboratory of Bioenergetics and Oxidative Stress, Brazil.Fil: Gaspar, Joana M. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.Fil: Gaspar, Joana M. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.https://orcid.org/0000-0002-5127-5640https://orcid.org/0000-0001-9032-1411https://orcid.org/0000-0003-3151-42342024-03-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfSchmitt, L.O.; Blanco, A.; Lima, S.V.; Mancini, G.; Mendes, N.F.; Latini, A.; Gaspar, J.M. Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function. Metabolites 2024, 14, 161. https:// doi.org/10.3390/metabo14030161http://hdl.handle.net/11086/5525682218-1989https://www.mdpi.com/2218-1989/14/3/161https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10971872/https://doi.org/10.3390/metabo14030161enginfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2025-09-29T13:44:19Zoai:rdu.unc.edu.ar:11086/552568Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-09-29 13:44:20.007Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse
dc.title.none.fl_str_mv Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
title Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
spellingShingle Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
Schmitt, Luisa O.
Obesity
Inflammation
Mitochondria
Saturated fatty acids
Astrocytes
title_short Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
title_full Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
title_fullStr Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
title_full_unstemmed Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
title_sort Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
dc.creator.none.fl_str_mv Schmitt, Luisa O.
Blanco, Antonella
Lima, Sheila V.
Mancini, Gianni
Mendes, Natalia F.
Latini, Alexandra
Gaspar, Joana M.
author Schmitt, Luisa O.
author_facet Schmitt, Luisa O.
Blanco, Antonella
Lima, Sheila V.
Mancini, Gianni
Mendes, Natalia F.
Latini, Alexandra
Gaspar, Joana M.
author_role author
author2 Blanco, Antonella
Lima, Sheila V.
Mancini, Gianni
Mendes, Natalia F.
Latini, Alexandra
Gaspar, Joana M.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv https://orcid.org/0000-0002-5127-5640
https://orcid.org/0000-0001-9032-1411
https://orcid.org/0000-0003-3151-4234
dc.subject.none.fl_str_mv Obesity
Inflammation
Mitochondria
Saturated fatty acids
Astrocytes
topic Obesity
Inflammation
Mitochondria
Saturated fatty acids
Astrocytes
dc.description.none.fl_txt_mv Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.
Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.
Fil: Blanco, Antonella. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica, Argentina.
Fil: Blanco, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina.
Fil: Blanco, Antonella. Federal University of Santa Catarina. School of Biological Sciences. Department of Biochemistry Laboratory of Bioenergetics and Oxidative Stress, Brazil.
Fil: Lima, Sheila V. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.
Fil: Mancini, Gianni. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.
Fil: Mendes, Natalia F. University of Campinas. School of Medical Sciences. Department of Translational Medicine (Section of Pharmacology), Brazil.
Fil: Latini, Alexandra. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.
Fil: Latini, Alexandra. Federal University of Santa Catarina. School of Biological Sciences. Department of Biochemistry Laboratory of Bioenergetics and Oxidative Stress, Brazil.
Fil: Gaspar, Joana M. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.
Fil: Gaspar, Joana M. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.
Consumption of high-fat diets (HFD) is associated with brain alterations, including changes in feeding behavior, cognitive decline, and dementia. Astrocytes play a role in HFD-induced neuroinflammation and brain dysfunction; however, this process is not entirely understood. We hypothesized that exposure to saturated fatty acids can compromise astrocyte viability and mitochondrial function. The C6 (astrocytes) cell line was treated with palmitate or stearate (200 μM and 400 μM) for 6 h. Cell viability, morphology, inflammatory markers, and oxidative stress were evaluated. To assess mitochondrial function, various parameters were measured (membrane potential, mass, respiration, and complex activities). We observed that 6 h of treatment with 400 μM palmitate decreased cell viability, and treatment with 200 μM palmitate changed the astrocyte morphology. Palmitate increased inflammatory markers (TNF-α and IL6) but did not induce oxidative stress. Palmitate significantly decreased the mitochondrial membrane potential and mitochondrial mass. Complex I activity also decreased in palmitate-treated cells; however, no changes were observed in mitochondrial respiration. In conclusion, palmitate, a saturated fatty acid, induces inflammation and impairs mitochondrial function, leading to reduced astrocytic cell viability and changes in cellular morphology. Our study provides valuable insights into the potential mechanisms underlying the relationship between saturated fatty acids, astrocytes, and mitochondrial function in obesity-related brain dysfunction.
info:eu-repo/semantics/publishedVersion
Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.
Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.
Fil: Blanco, Antonella. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Departamento de Bioquímica Clínica, Argentina.
Fil: Blanco, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigaciones en Bioquímica Clínica e Inmunología, Argentina.
Fil: Blanco, Antonella. Federal University of Santa Catarina. School of Biological Sciences. Department of Biochemistry Laboratory of Bioenergetics and Oxidative Stress, Brazil.
Fil: Lima, Sheila V. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.
Fil: Mancini, Gianni. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.
Fil: Mendes, Natalia F. University of Campinas. School of Medical Sciences. Department of Translational Medicine (Section of Pharmacology), Brazil.
Fil: Latini, Alexandra. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.
Fil: Latini, Alexandra. Federal University of Santa Catarina. School of Biological Sciences. Department of Biochemistry Laboratory of Bioenergetics and Oxidative Stress, Brazil.
Fil: Gaspar, Joana M. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.
Fil: Gaspar, Joana M. Federal University of Santa Catarina. Graduate Program in Biochemistry, Brazil.
description Fil: Schmitt, Luisa O. Federal University of Santa Catarina. Laboratory of Neuroimmune-Metabolism, Federal, Brazil.
publishDate 2024
dc.date.none.fl_str_mv 2024-03-12
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
status_str publishedVersion
format article
dc.identifier.none.fl_str_mv Schmitt, L.O.; Blanco, A.; Lima, S.V.; Mancini, G.; Mendes, N.F.; Latini, A.; Gaspar, J.M. Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function. Metabolites 2024, 14, 161. https:// doi.org/10.3390/metabo14030161
http://hdl.handle.net/11086/552568
2218-1989
https://www.mdpi.com/2218-1989/14/3/161
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10971872/
https://doi.org/10.3390/metabo14030161
identifier_str_mv Schmitt, L.O.; Blanco, A.; Lima, S.V.; Mancini, G.; Mendes, N.F.; Latini, A.; Gaspar, J.M. Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function. Metabolites 2024, 14, 161. https:// doi.org/10.3390/metabo14030161
2218-1989
url http://hdl.handle.net/11086/552568
https://www.mdpi.com/2218-1989/14/3/161
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10971872/
https://doi.org/10.3390/metabo14030161
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Repositorio Digital Universitario (UNC)
instname:Universidad Nacional de Córdoba
instacron:UNC
reponame_str Repositorio Digital Universitario (UNC)
collection Repositorio Digital Universitario (UNC)
instname_str Universidad Nacional de Córdoba
instacron_str UNC
institution UNC
repository.name.fl_str_mv Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba
repository.mail.fl_str_mv oca.unc@gmail.com
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score 13.070432

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