Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function

Autores
Schmitt, Luisa O.; Blanco, Antonella; Lima, Sheila V.; Mancini, Gianni; Mendes, Natalia F.; Latini, Alexandra; Gaspar, Joana M.
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Consumption of high-fat diets (HFD) is associated with brain alterations, including changes in feeding behavior, cognitive decline, and dementia. Astrocytes play a role in HFD-induced neuroinflammation and brain dysfunction; however, this process is not entirely understood. We hypothesized that exposure to saturated fatty acids can compromise astrocyte viability and mitochondrial function. The C6 (astrocytes) cell line was treated with palmitate or stearate (200 µM and 400 µM) for 6 h. Cell viability, morphology, inflammatory markers, and oxidative stress were evaluated. To assess mitochondrial function, various parameters were measured (membrane potential, mass, respiration, and complex activities). We observed that 6 h of treatment with 400 µM palmitate decreased cell viability, and treatment with 200 µM palmitate changed the astrocyte morphology. Palmitate increased inflammatory markers (TNF-α and IL6) but did not induce oxidative stress. Palmitate significantly decreased the mitochondrial membrane potential and mitochondrial mass. Complex I activity also decreased in palmitate-treated cells; however, no changes were observed in mitochondrial respiration. In conclusion, palmitate, a saturated fatty acid, induces inflammation and impairs mitochondrial function, leading to reduced astrocytic cell viability and changes in cellular morphology. Our study provides valuable insights into the potential mechanisms underlying the relationship between saturated fatty acids, astrocytes, and mitochondrial function in obesity-related brain dysfunction.
Fil: Schmitt, Luisa O.. Universidade Federal Da Santa Catarina. Centro de Ciencias Biológicas.; Brasil
Fil: Blanco, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Lima, Sheila V.. Universidade Federal Da Santa Catarina. Centro de Ciencias Biológicas.; Brasil
Fil: Mancini, Gianni. Universidade Federal Da Santa Catarina. Centro de Ciencias Biológicas.; Brasil
Fil: Mendes, Natalia F.. Universidade Estadual de Campinas; Brasil
Fil: Latini, Alexandra. Universidade Federal Da Santa Catarina. Centro de Ciencias Biológicas.; Brasil
Fil: Gaspar, Joana M.. Universidade Federal Da Santa Catarina. Centro de Ciencias Biológicas.; Brasil
Materia
obesity
inflammation
mitochondria
saturated fatty acids
astrocytes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/262885
Acceder
id CONICETDig_5bc728e301f8ec309fc321884d69b51a
oai_identifier_str oai:ri.conicet.gov.ar:11336/262885
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial FunctionSchmitt, Luisa O.Blanco, AntonellaLima, Sheila V.Mancini, GianniMendes, Natalia F.Latini, AlexandraGaspar, Joana M.obesityinflammationmitochondriasaturated fatty acidsastrocyteshttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Consumption of high-fat diets (HFD) is associated with brain alterations, including changes in feeding behavior, cognitive decline, and dementia. Astrocytes play a role in HFD-induced neuroinflammation and brain dysfunction; however, this process is not entirely understood. We hypothesized that exposure to saturated fatty acids can compromise astrocyte viability and mitochondrial function. The C6 (astrocytes) cell line was treated with palmitate or stearate (200 µM and 400 µM) for 6 h. Cell viability, morphology, inflammatory markers, and oxidative stress were evaluated. To assess mitochondrial function, various parameters were measured (membrane potential, mass, respiration, and complex activities). We observed that 6 h of treatment with 400 µM palmitate decreased cell viability, and treatment with 200 µM palmitate changed the astrocyte morphology. Palmitate increased inflammatory markers (TNF-α and IL6) but did not induce oxidative stress. Palmitate significantly decreased the mitochondrial membrane potential and mitochondrial mass. Complex I activity also decreased in palmitate-treated cells; however, no changes were observed in mitochondrial respiration. In conclusion, palmitate, a saturated fatty acid, induces inflammation and impairs mitochondrial function, leading to reduced astrocytic cell viability and changes in cellular morphology. Our study provides valuable insights into the potential mechanisms underlying the relationship between saturated fatty acids, astrocytes, and mitochondrial function in obesity-related brain dysfunction.Fil: Schmitt, Luisa O.. Universidade Federal Da Santa Catarina. Centro de Ciencias Biológicas.; BrasilFil: Blanco, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; ArgentinaFil: Lima, Sheila V.. Universidade Federal Da Santa Catarina. Centro de Ciencias Biológicas.; BrasilFil: Mancini, Gianni. Universidade Federal Da Santa Catarina. Centro de Ciencias Biológicas.; BrasilFil: Mendes, Natalia F.. Universidade Estadual de Campinas; BrasilFil: Latini, Alexandra. Universidade Federal Da Santa Catarina. Centro de Ciencias Biológicas.; BrasilFil: Gaspar, Joana M.. Universidade Federal Da Santa Catarina. Centro de Ciencias Biológicas.; BrasilMDPI2024-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/262885Schmitt, Luisa O.; Blanco, Antonella; Lima, Sheila V.; Mancini, Gianni; Mendes, Natalia F.; et al.; Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function; MDPI; Metabolites; 14; 3; 3-2024; 1-182218-1989CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2218-1989/14/3/161info:eu-repo/semantics/altIdentifier/doi/10.3390/metabo14030161info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:57:30Zoai:ri.conicet.gov.ar:11336/262885instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:57:30.994CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
title Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
spellingShingle Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
Schmitt, Luisa O.
obesity
inflammation
mitochondria
saturated fatty acids
astrocytes
title_short Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
title_full Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
title_fullStr Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
title_full_unstemmed Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
title_sort Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function
dc.creator.none.fl_str_mv Schmitt, Luisa O.
Blanco, Antonella
Lima, Sheila V.
Mancini, Gianni
Mendes, Natalia F.
Latini, Alexandra
Gaspar, Joana M.
author Schmitt, Luisa O.
author_facet Schmitt, Luisa O.
Blanco, Antonella
Lima, Sheila V.
Mancini, Gianni
Mendes, Natalia F.
Latini, Alexandra
Gaspar, Joana M.
author_role author
author2 Blanco, Antonella
Lima, Sheila V.
Mancini, Gianni
Mendes, Natalia F.
Latini, Alexandra
Gaspar, Joana M.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv obesity
inflammation
mitochondria
saturated fatty acids
astrocytes
topic obesity
inflammation
mitochondria
saturated fatty acids
astrocytes
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Consumption of high-fat diets (HFD) is associated with brain alterations, including changes in feeding behavior, cognitive decline, and dementia. Astrocytes play a role in HFD-induced neuroinflammation and brain dysfunction; however, this process is not entirely understood. We hypothesized that exposure to saturated fatty acids can compromise astrocyte viability and mitochondrial function. The C6 (astrocytes) cell line was treated with palmitate or stearate (200 µM and 400 µM) for 6 h. Cell viability, morphology, inflammatory markers, and oxidative stress were evaluated. To assess mitochondrial function, various parameters were measured (membrane potential, mass, respiration, and complex activities). We observed that 6 h of treatment with 400 µM palmitate decreased cell viability, and treatment with 200 µM palmitate changed the astrocyte morphology. Palmitate increased inflammatory markers (TNF-α and IL6) but did not induce oxidative stress. Palmitate significantly decreased the mitochondrial membrane potential and mitochondrial mass. Complex I activity also decreased in palmitate-treated cells; however, no changes were observed in mitochondrial respiration. In conclusion, palmitate, a saturated fatty acid, induces inflammation and impairs mitochondrial function, leading to reduced astrocytic cell viability and changes in cellular morphology. Our study provides valuable insights into the potential mechanisms underlying the relationship between saturated fatty acids, astrocytes, and mitochondrial function in obesity-related brain dysfunction.
Fil: Schmitt, Luisa O.. Universidade Federal Da Santa Catarina. Centro de Ciencias Biológicas.; Brasil
Fil: Blanco, Antonella. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Córdoba. Centro de Investigaciones en Bioquímica Clínica e Inmunología; Argentina
Fil: Lima, Sheila V.. Universidade Federal Da Santa Catarina. Centro de Ciencias Biológicas.; Brasil
Fil: Mancini, Gianni. Universidade Federal Da Santa Catarina. Centro de Ciencias Biológicas.; Brasil
Fil: Mendes, Natalia F.. Universidade Estadual de Campinas; Brasil
Fil: Latini, Alexandra. Universidade Federal Da Santa Catarina. Centro de Ciencias Biológicas.; Brasil
Fil: Gaspar, Joana M.. Universidade Federal Da Santa Catarina. Centro de Ciencias Biológicas.; Brasil
description Consumption of high-fat diets (HFD) is associated with brain alterations, including changes in feeding behavior, cognitive decline, and dementia. Astrocytes play a role in HFD-induced neuroinflammation and brain dysfunction; however, this process is not entirely understood. We hypothesized that exposure to saturated fatty acids can compromise astrocyte viability and mitochondrial function. The C6 (astrocytes) cell line was treated with palmitate or stearate (200 µM and 400 µM) for 6 h. Cell viability, morphology, inflammatory markers, and oxidative stress were evaluated. To assess mitochondrial function, various parameters were measured (membrane potential, mass, respiration, and complex activities). We observed that 6 h of treatment with 400 µM palmitate decreased cell viability, and treatment with 200 µM palmitate changed the astrocyte morphology. Palmitate increased inflammatory markers (TNF-α and IL6) but did not induce oxidative stress. Palmitate significantly decreased the mitochondrial membrane potential and mitochondrial mass. Complex I activity also decreased in palmitate-treated cells; however, no changes were observed in mitochondrial respiration. In conclusion, palmitate, a saturated fatty acid, induces inflammation and impairs mitochondrial function, leading to reduced astrocytic cell viability and changes in cellular morphology. Our study provides valuable insights into the potential mechanisms underlying the relationship between saturated fatty acids, astrocytes, and mitochondrial function in obesity-related brain dysfunction.
publishDate 2024
dc.date.none.fl_str_mv 2024-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/262885
Schmitt, Luisa O.; Blanco, Antonella; Lima, Sheila V.; Mancini, Gianni; Mendes, Natalia F.; et al.; Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function; MDPI; Metabolites; 14; 3; 3-2024; 1-18
2218-1989
CONICET Digital
CONICET
url http://hdl.handle.net/11336/262885
identifier_str_mv Schmitt, Luisa O.; Blanco, Antonella; Lima, Sheila V.; Mancini, Gianni; Mendes, Natalia F.; et al.; Palmitate Compromises C6 Astrocytic Cell Viability and Mitochondrial Function; MDPI; Metabolites; 14; 3; 3-2024; 1-18
2218-1989
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://www.mdpi.com/2218-1989/14/3/161
info:eu-repo/semantics/altIdentifier/doi/10.3390/metabo14030161
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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