Intercellular mitochondrial transfer through nanotubules is promoted by cyclic amp (cAMP) in rat astrocytes and human glioblastoma cells

Autores
Helfenberger, Katia Estefanía; Finocchietto, Paola Vanesa; Duarte, Alejandra Beatriz; Fuentes, Federico; Poderoso, Juan José; Gelpi, Ricardo Jorge; Poderoso, Cecilia
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Introduction: Tunneling nanotubes (TnTs) have been described as communications between cells, made of F-actin fibers of nanometric diameter. Mitochondria are transferred by TnTs in numerous cellular lines (1). Mitochondrial passage to tumor cells restores oxidative metabolism, decreases tumorigenic potential, as cAMP tumor cells treatment does (2). Our goal was to analyze mitochondrial trafficking through TnTs in normal and cancerous astrocytes and the effect of cAMP (which promotes astrocytes stellation). Materials & Methods: We used rat astrocytes and human glioblastoma U87 cells. Mitochondria and actin were probed with a specific-targeted green fluorescent protein and rhodamine phalloidin, respectively. Astrocytes and U87 were incubated with 8Br-cAMP (cAMP analogue). We analyzed images by confocal miscroscopy and measured width of actin connections between cells. Results: We analyzed each culture separately; astrocytes and U87 establish thick projections with mitochondria inside but treatment with cAMP promotes an increase of TnTs-like connections with mitochondria inside (control vs cAMP: astrocytes: 2.07±0.71 vs 0.85±0.21 μm, ***p<0.05, and U87: 2.69±1.40 vs.0.84±0.22 μm, *p<0.05, ±SD, ANOVA, Tukey?s test). Conclusion: cAMP promotes TnTs-like structures and mitochondrial passage within them in both normal astrocytes and glioblastoma cells; suggesting a possible role for intercellular mitochondrial transfer through TnTs induced by cAMP, in stellation process.
Fil: Helfenberger, Katia Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Finocchietto, Paola Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Duarte, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Fuentes, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Poderoso, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Gelpi, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Poderoso, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
8th World Congress on Targeting Mitochondria
Berlin
Alemania
World Mitochondria Society
Materia
ASTROCYTES
NANOTUBULES
MITOCHONDRIA
GLIOBLASTOMA
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/263361
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/263361
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Intercellular mitochondrial transfer through nanotubules is promoted by cyclic amp (cAMP) in rat astrocytes and human glioblastoma cellsHelfenberger, Katia EstefaníaFinocchietto, Paola VanesaDuarte, Alejandra BeatrizFuentes, FedericoPoderoso, Juan JoséGelpi, Ricardo JorgePoderoso, CeciliaASTROCYTESNANOTUBULESMITOCHONDRIAGLIOBLASTOMAhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Introduction: Tunneling nanotubes (TnTs) have been described as communications between cells, made of F-actin fibers of nanometric diameter. Mitochondria are transferred by TnTs in numerous cellular lines (1). Mitochondrial passage to tumor cells restores oxidative metabolism, decreases tumorigenic potential, as cAMP tumor cells treatment does (2). Our goal was to analyze mitochondrial trafficking through TnTs in normal and cancerous astrocytes and the effect of cAMP (which promotes astrocytes stellation). Materials & Methods: We used rat astrocytes and human glioblastoma U87 cells. Mitochondria and actin were probed with a specific-targeted green fluorescent protein and rhodamine phalloidin, respectively. Astrocytes and U87 were incubated with 8Br-cAMP (cAMP analogue). We analyzed images by confocal miscroscopy and measured width of actin connections between cells. Results: We analyzed each culture separately; astrocytes and U87 establish thick projections with mitochondria inside but treatment with cAMP promotes an increase of TnTs-like connections with mitochondria inside (control vs cAMP: astrocytes: 2.07±0.71 vs 0.85±0.21 μm, ***p<0.05, and U87: 2.69±1.40 vs.0.84±0.22 μm, *p<0.05, ±SD, ANOVA, Tukey?s test). Conclusion: cAMP promotes TnTs-like structures and mitochondrial passage within them in both normal astrocytes and glioblastoma cells; suggesting a possible role for intercellular mitochondrial transfer through TnTs induced by cAMP, in stellation process.Fil: Helfenberger, Katia Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; ArgentinaFil: Finocchietto, Paola Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Duarte, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Fuentes, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; ArgentinaFil: Poderoso, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; ArgentinaFil: Gelpi, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Poderoso, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina8th World Congress on Targeting MitochondriaBerlinAlemaniaWorld Mitochondria SocietyWorld Mitochondria Society2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectCongresoJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/263361Intercellular mitochondrial transfer through nanotubules is promoted by cyclic amp (cAMP) in rat astrocytes and human glioblastoma cells; 8th World Congress on Targeting Mitochondria; Berlin; Alemania; 2017; 119-119CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.18143/JWMS_v3i1info:eu-repo/semantics/altIdentifier/url/https://wms-site.com/71-archives/679-wms-archive-2017Internacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:47:17Zoai:ri.conicet.gov.ar:11336/263361instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:47:17.631CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Intercellular mitochondrial transfer through nanotubules is promoted by cyclic amp (cAMP) in rat astrocytes and human glioblastoma cells
title Intercellular mitochondrial transfer through nanotubules is promoted by cyclic amp (cAMP) in rat astrocytes and human glioblastoma cells
spellingShingle Intercellular mitochondrial transfer through nanotubules is promoted by cyclic amp (cAMP) in rat astrocytes and human glioblastoma cells
Helfenberger, Katia Estefanía
ASTROCYTES
NANOTUBULES
MITOCHONDRIA
GLIOBLASTOMA
title_short Intercellular mitochondrial transfer through nanotubules is promoted by cyclic amp (cAMP) in rat astrocytes and human glioblastoma cells
title_full Intercellular mitochondrial transfer through nanotubules is promoted by cyclic amp (cAMP) in rat astrocytes and human glioblastoma cells
title_fullStr Intercellular mitochondrial transfer through nanotubules is promoted by cyclic amp (cAMP) in rat astrocytes and human glioblastoma cells
title_full_unstemmed Intercellular mitochondrial transfer through nanotubules is promoted by cyclic amp (cAMP) in rat astrocytes and human glioblastoma cells
title_sort Intercellular mitochondrial transfer through nanotubules is promoted by cyclic amp (cAMP) in rat astrocytes and human glioblastoma cells
dc.creator.none.fl_str_mv Helfenberger, Katia Estefanía
Finocchietto, Paola Vanesa
Duarte, Alejandra Beatriz
Fuentes, Federico
Poderoso, Juan José
Gelpi, Ricardo Jorge
Poderoso, Cecilia
author Helfenberger, Katia Estefanía
author_facet Helfenberger, Katia Estefanía
Finocchietto, Paola Vanesa
Duarte, Alejandra Beatriz
Fuentes, Federico
Poderoso, Juan José
Gelpi, Ricardo Jorge
Poderoso, Cecilia
author_role author
author2 Finocchietto, Paola Vanesa
Duarte, Alejandra Beatriz
Fuentes, Federico
Poderoso, Juan José
Gelpi, Ricardo Jorge
Poderoso, Cecilia
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv ASTROCYTES
NANOTUBULES
MITOCHONDRIA
GLIOBLASTOMA
topic ASTROCYTES
NANOTUBULES
MITOCHONDRIA
GLIOBLASTOMA
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Introduction: Tunneling nanotubes (TnTs) have been described as communications between cells, made of F-actin fibers of nanometric diameter. Mitochondria are transferred by TnTs in numerous cellular lines (1). Mitochondrial passage to tumor cells restores oxidative metabolism, decreases tumorigenic potential, as cAMP tumor cells treatment does (2). Our goal was to analyze mitochondrial trafficking through TnTs in normal and cancerous astrocytes and the effect of cAMP (which promotes astrocytes stellation). Materials & Methods: We used rat astrocytes and human glioblastoma U87 cells. Mitochondria and actin were probed with a specific-targeted green fluorescent protein and rhodamine phalloidin, respectively. Astrocytes and U87 were incubated with 8Br-cAMP (cAMP analogue). We analyzed images by confocal miscroscopy and measured width of actin connections between cells. Results: We analyzed each culture separately; astrocytes and U87 establish thick projections with mitochondria inside but treatment with cAMP promotes an increase of TnTs-like connections with mitochondria inside (control vs cAMP: astrocytes: 2.07±0.71 vs 0.85±0.21 μm, ***p<0.05, and U87: 2.69±1.40 vs.0.84±0.22 μm, *p<0.05, ±SD, ANOVA, Tukey?s test). Conclusion: cAMP promotes TnTs-like structures and mitochondrial passage within them in both normal astrocytes and glioblastoma cells; suggesting a possible role for intercellular mitochondrial transfer through TnTs induced by cAMP, in stellation process.
Fil: Helfenberger, Katia Estefanía. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
Fil: Finocchietto, Paola Vanesa. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Duarte, Alejandra Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Fuentes, Federico. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Medicina Experimental. Academia Nacional de Medicina de Buenos Aires. Instituto de Medicina Experimental; Argentina
Fil: Poderoso, Juan José. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Inmunología, Genética y Metabolismo. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Inmunología, Genética y Metabolismo; Argentina
Fil: Gelpi, Ricardo Jorge. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Poderoso, Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Investigaciones Biomédicas. Universidad de Buenos Aires. Facultad de Medicina. Instituto de Investigaciones Biomédicas; Argentina
8th World Congress on Targeting Mitochondria
Berlin
Alemania
World Mitochondria Society
description Introduction: Tunneling nanotubes (TnTs) have been described as communications between cells, made of F-actin fibers of nanometric diameter. Mitochondria are transferred by TnTs in numerous cellular lines (1). Mitochondrial passage to tumor cells restores oxidative metabolism, decreases tumorigenic potential, as cAMP tumor cells treatment does (2). Our goal was to analyze mitochondrial trafficking through TnTs in normal and cancerous astrocytes and the effect of cAMP (which promotes astrocytes stellation). Materials & Methods: We used rat astrocytes and human glioblastoma U87 cells. Mitochondria and actin were probed with a specific-targeted green fluorescent protein and rhodamine phalloidin, respectively. Astrocytes and U87 were incubated with 8Br-cAMP (cAMP analogue). We analyzed images by confocal miscroscopy and measured width of actin connections between cells. Results: We analyzed each culture separately; astrocytes and U87 establish thick projections with mitochondria inside but treatment with cAMP promotes an increase of TnTs-like connections with mitochondria inside (control vs cAMP: astrocytes: 2.07±0.71 vs 0.85±0.21 μm, ***p<0.05, and U87: 2.69±1.40 vs.0.84±0.22 μm, *p<0.05, ±SD, ANOVA, Tukey?s test). Conclusion: cAMP promotes TnTs-like structures and mitochondrial passage within them in both normal astrocytes and glioblastoma cells; suggesting a possible role for intercellular mitochondrial transfer through TnTs induced by cAMP, in stellation process.
publishDate 2017
dc.date.none.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Congreso
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/263361
Intercellular mitochondrial transfer through nanotubules is promoted by cyclic amp (cAMP) in rat astrocytes and human glioblastoma cells; 8th World Congress on Targeting Mitochondria; Berlin; Alemania; 2017; 119-119
CONICET Digital
CONICET
url http://hdl.handle.net/11336/263361
identifier_str_mv Intercellular mitochondrial transfer through nanotubules is promoted by cyclic amp (cAMP) in rat astrocytes and human glioblastoma cells; 8th World Congress on Targeting Mitochondria; Berlin; Alemania; 2017; 119-119
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://dx.doi.org/10.18143/JWMS_v3i1
info:eu-repo/semantics/altIdentifier/url/https://wms-site.com/71-archives/679-wms-archive-2017
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
dc.coverage.none.fl_str_mv Internacional
dc.publisher.none.fl_str_mv World Mitochondria Society
publisher.none.fl_str_mv World Mitochondria Society
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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