Brief ethanol doses modifies 5HT levels at Raphe obscurus in neonate rats

Autores
Macchione, Ana Fabiola; Trujillo, Veronica; Anastasia, Agustín; Molina, Juan Carlos
Año de publicación
2017
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
Fil: Macchione, Ana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Fil: Macchione, Ana Fabiola. Universidad Nacional de Córdoba. Facultad de Psicologías; Argentina.
Fil: Trujillo, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Fil: Anastasia, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Fil: Molina, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Fil: Molina, Juan Carlos. Universidad Nacional de Córdoba. Facultad de Psicologías; Argentina.
Ethanol is the most commonly abused substance that impacts the humans during early ontogeny (gestation and lactation). Ethanol effects include alterations in brain development, learning and memory, cognitive abilities and neurovegetative functions such as breathing. A major target of ethanol in the central nervous system is the serotonin (5HT) neurons involved on the respiratory regulation. Preclinical studies employing chronic and severe ethanol consumption models during pregnancy and lactation, showed a decrease in the number of 5HT-neurons and 5HT levels in the raphe system. However, the status of the serotonergic system in animal models with moderate and neonatal ethanol exposure are understudied. Thus, the main goal of this study was to evaluate 5HT levels in the raphe obscurus (ROb) nucleus in neonate rats, as a function of moderate ethanol or vehicle exposure (2.0 or 0.0g/kg, intragastrically) at postnatal days (PD) 3, 5 and 7. At PD 9, pups were intoxicated or vehicle administrated. Ninety minutes after, all pups were sacrificed and 5HT levels were immunodetected at the ROb. A significant reduction of 5HT levels was observed in pups exposed to ethanol for the first time at PD 9 and vehicle-pretreated at PDs 3, 5 & 7. Interestingly, a similar decrease in 5HT levels was also found in ethanol-pretreated pups evaluated under sobriety at PD 9. These results indicate a profound effect of ethanol upon on 5HT levels at ROb, nucleus specifically involved in breathing regulation. These data indicate that perinatal ethanol exposure is enough to alter 5HT levels in the Rob. These results became important when associating the function of the ROb on respiratory response and breathing disturbances commonly observed in neonate rats and humans, such as Sudden Infant Death Syndrome.
https://drive.google.com/file/d/1ANKFVSKnbUO3PJX7jV94t_L1nTDKgU-d/view
Fil: Macchione, Ana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Fil: Macchione, Ana Fabiola. Universidad Nacional de Córdoba. Facultad de Psicologías; Argentina.
Fil: Trujillo, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Fil: Anastasia, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Fil: Molina, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Fil: Molina, Juan Carlos. Universidad Nacional de Córdoba. Facultad de Psicologías; Argentina.
Neurociencias (incluye Psicofiosiología)
Materia
Serotonina
Raphe
Etanol
Medicina
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
Repositorio Digital Universitario (UNC)
Institución
Universidad Nacional de Córdoba
OAI Identificador
oai:rdu.unc.edu.ar:11086/555206
Acceder
id RDUUNC_693c7706bdb8c13a20f08db9f877db0c
oai_identifier_str oai:rdu.unc.edu.ar:11086/555206
network_acronym_str RDUUNC
repository_id_str 2572
network_name_str Repositorio Digital Universitario (UNC)
spelling Brief ethanol doses modifies 5HT levels at Raphe obscurus in neonate ratsMacchione, Ana FabiolaTrujillo, VeronicaAnastasia, AgustínMolina, Juan CarlosSerotoninaRapheEtanolMedicinaFil: Macchione, Ana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.Fil: Macchione, Ana Fabiola. Universidad Nacional de Córdoba. Facultad de Psicologías; Argentina.Fil: Trujillo, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.Fil: Anastasia, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.Fil: Molina, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.Fil: Molina, Juan Carlos. Universidad Nacional de Córdoba. Facultad de Psicologías; Argentina.Ethanol is the most commonly abused substance that impacts the humans during early ontogeny (gestation and lactation). Ethanol effects include alterations in brain development, learning and memory, cognitive abilities and neurovegetative functions such as breathing. A major target of ethanol in the central nervous system is the serotonin (5HT) neurons involved on the respiratory regulation. Preclinical studies employing chronic and severe ethanol consumption models during pregnancy and lactation, showed a decrease in the number of 5HT-neurons and 5HT levels in the raphe system. However, the status of the serotonergic system in animal models with moderate and neonatal ethanol exposure are understudied. Thus, the main goal of this study was to evaluate 5HT levels in the raphe obscurus (ROb) nucleus in neonate rats, as a function of moderate ethanol or vehicle exposure (2.0 or 0.0g/kg, intragastrically) at postnatal days (PD) 3, 5 and 7. At PD 9, pups were intoxicated or vehicle administrated. Ninety minutes after, all pups were sacrificed and 5HT levels were immunodetected at the ROb. A significant reduction of 5HT levels was observed in pups exposed to ethanol for the first time at PD 9 and vehicle-pretreated at PDs 3, 5 & 7. Interestingly, a similar decrease in 5HT levels was also found in ethanol-pretreated pups evaluated under sobriety at PD 9. These results indicate a profound effect of ethanol upon on 5HT levels at ROb, nucleus specifically involved in breathing regulation. These data indicate that perinatal ethanol exposure is enough to alter 5HT levels in the Rob. These results became important when associating the function of the ROb on respiratory response and breathing disturbances commonly observed in neonate rats and humans, such as Sudden Infant Death Syndrome.https://drive.google.com/file/d/1ANKFVSKnbUO3PJX7jV94t_L1nTDKgU-d/viewFil: Macchione, Ana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.Fil: Macchione, Ana Fabiola. Universidad Nacional de Córdoba. Facultad de Psicologías; Argentina.Fil: Trujillo, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.Fil: Anastasia, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.Fil: Molina, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.Fil: Molina, Juan Carlos. Universidad Nacional de Córdoba. Facultad de Psicologías; Argentina.Neurociencias (incluye Psicofiosiología)2017info:eu-repo/semantics/conferenceObjectinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdf1669-9106http://hdl.handle.net/11086/555206enginfo:eu-repo/semantics/openAccessreponame:Repositorio Digital Universitario (UNC)instname:Universidad Nacional de Córdobainstacron:UNC2025-09-29T13:41:35Zoai:rdu.unc.edu.ar:11086/555206Institucionalhttps://rdu.unc.edu.ar/Universidad públicaNo correspondehttp://rdu.unc.edu.ar/oai/snrdoca.unc@gmail.comArgentinaNo correspondeNo correspondeNo correspondeopendoar:25722025-09-29 13:41:35.386Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdobafalse
dc.title.none.fl_str_mv Brief ethanol doses modifies 5HT levels at Raphe obscurus in neonate rats
title Brief ethanol doses modifies 5HT levels at Raphe obscurus in neonate rats
spellingShingle Brief ethanol doses modifies 5HT levels at Raphe obscurus in neonate rats
Macchione, Ana Fabiola
Serotonina
Raphe
Etanol
Medicina
title_short Brief ethanol doses modifies 5HT levels at Raphe obscurus in neonate rats
title_full Brief ethanol doses modifies 5HT levels at Raphe obscurus in neonate rats
title_fullStr Brief ethanol doses modifies 5HT levels at Raphe obscurus in neonate rats
title_full_unstemmed Brief ethanol doses modifies 5HT levels at Raphe obscurus in neonate rats
title_sort Brief ethanol doses modifies 5HT levels at Raphe obscurus in neonate rats
dc.creator.none.fl_str_mv Macchione, Ana Fabiola
Trujillo, Veronica
Anastasia, Agustín
Molina, Juan Carlos
author Macchione, Ana Fabiola
author_facet Macchione, Ana Fabiola
Trujillo, Veronica
Anastasia, Agustín
Molina, Juan Carlos
author_role author
author2 Trujillo, Veronica
Anastasia, Agustín
Molina, Juan Carlos
author2_role author
author
author
dc.subject.none.fl_str_mv Serotonina
Raphe
Etanol
Medicina
topic Serotonina
Raphe
Etanol
Medicina
dc.description.none.fl_txt_mv Fil: Macchione, Ana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Fil: Macchione, Ana Fabiola. Universidad Nacional de Córdoba. Facultad de Psicologías; Argentina.
Fil: Trujillo, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Fil: Anastasia, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Fil: Molina, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Fil: Molina, Juan Carlos. Universidad Nacional de Córdoba. Facultad de Psicologías; Argentina.
Ethanol is the most commonly abused substance that impacts the humans during early ontogeny (gestation and lactation). Ethanol effects include alterations in brain development, learning and memory, cognitive abilities and neurovegetative functions such as breathing. A major target of ethanol in the central nervous system is the serotonin (5HT) neurons involved on the respiratory regulation. Preclinical studies employing chronic and severe ethanol consumption models during pregnancy and lactation, showed a decrease in the number of 5HT-neurons and 5HT levels in the raphe system. However, the status of the serotonergic system in animal models with moderate and neonatal ethanol exposure are understudied. Thus, the main goal of this study was to evaluate 5HT levels in the raphe obscurus (ROb) nucleus in neonate rats, as a function of moderate ethanol or vehicle exposure (2.0 or 0.0g/kg, intragastrically) at postnatal days (PD) 3, 5 and 7. At PD 9, pups were intoxicated or vehicle administrated. Ninety minutes after, all pups were sacrificed and 5HT levels were immunodetected at the ROb. A significant reduction of 5HT levels was observed in pups exposed to ethanol for the first time at PD 9 and vehicle-pretreated at PDs 3, 5 & 7. Interestingly, a similar decrease in 5HT levels was also found in ethanol-pretreated pups evaluated under sobriety at PD 9. These results indicate a profound effect of ethanol upon on 5HT levels at ROb, nucleus specifically involved in breathing regulation. These data indicate that perinatal ethanol exposure is enough to alter 5HT levels in the Rob. These results became important when associating the function of the ROb on respiratory response and breathing disturbances commonly observed in neonate rats and humans, such as Sudden Infant Death Syndrome.
https://drive.google.com/file/d/1ANKFVSKnbUO3PJX7jV94t_L1nTDKgU-d/view
Fil: Macchione, Ana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Fil: Macchione, Ana Fabiola. Universidad Nacional de Córdoba. Facultad de Psicologías; Argentina.
Fil: Trujillo, Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Fil: Anastasia, Agustín. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Fil: Molina, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
Fil: Molina, Juan Carlos. Universidad Nacional de Córdoba. Facultad de Psicologías; Argentina.
Neurociencias (incluye Psicofiosiología)
description Fil: Macchione, Ana Fabiola. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina.
publishDate 2017
dc.date.none.fl_str_mv 2017
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info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.none.fl_str_mv 1669-9106
http://hdl.handle.net/11086/555206
identifier_str_mv 1669-9106
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dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Repositorio Digital Universitario (UNC)
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instname_str Universidad Nacional de Córdoba
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repository.name.fl_str_mv Repositorio Digital Universitario (UNC) - Universidad Nacional de Córdoba
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