Early Ethanol Preexposure Modifies Expression of the 5HT2A Receptor Promoting Long-Term Breathing Plasticity in Neonate Rats

Autores
Macchione, Ana Fabiola; Anunziata, Florencia; Sahonero, Milagros; Trujillo, Verónica; Molina, Juan Carlos
Año de publicación
2019
Idioma
inglés
Tipo de recurso
documento de conferencia
Estado
versión publicada
Descripción
EtOH´s effects upon respiration are attributed to central respiratory network disruptions, especially in the medullary serotonin (5HT) system. 5HT2A/2C receptors are involved in the reduction of the phrenic nerve activity and breathing depression. We hypothesize that early EtOH preexposure alters neonatal respiration through the 5HT system´s plasticity. Here, we evaluated breathing rates and the relative expression of 5HT 2A and 2C receptors in the brainstem as a function of EtOH preexposure in neonates. Pups received i.g administrations of 2.0 or 0.0g/kg EtOH at postnatal days (PD) 3, 5 and 7. At PD 9, breathing frequencies were recorded under normoxia or hypoxia. Brainstems were collected to quantified relative mRNA expression of 5HT 2A and 2C receptors, by qPCR. Under normoxia, EtOH preexposed pups (preEtOH) exhibited high 5HT2A expression levels and breathing depressions. An opposite phenomenon was observed in preEtOH pups tested under hypoxia. An exacerbated hyperventilation associated with low 5HT2A expression levels was found. No significant differences were found in 5HT2C expression levels. These results together with our previous findings that show changes in the raphe obscurus activation patterns, suggest that a brief EtOH preexposure is enough to induce 5HT system?s plasticity, disturbing neonatal breathing. The 5HT components mismatch may be associated with breathing disruptions commonly observed in human neonates, such as Sudden Infant Death Syndrome.
Fil: Macchione, Ana Fabiola. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Anunziata, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Sahonero, Milagros. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina
Fil: Trujillo, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Molina, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
XXXIV Reunión Anual de la Sociedad Argentina de Neurociencias
Villa Carlos Paz
Argentina
Sociedad Argentina de Investigación en Neurociencias
Materia
ETHANOL
BREATHING
5HT2A RECEPTOR
SEROTONIN
BRAINSTEM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/159648
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/159648
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Early Ethanol Preexposure Modifies Expression of the 5HT2A Receptor Promoting Long-Term Breathing Plasticity in Neonate RatsMacchione, Ana FabiolaAnunziata, FlorenciaSahonero, MilagrosTrujillo, VerónicaMolina, Juan CarlosETHANOLBREATHING5HT2A RECEPTORSEROTONINBRAINSTEMhttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3EtOH´s effects upon respiration are attributed to central respiratory network disruptions, especially in the medullary serotonin (5HT) system. 5HT2A/2C receptors are involved in the reduction of the phrenic nerve activity and breathing depression. We hypothesize that early EtOH preexposure alters neonatal respiration through the 5HT system´s plasticity. Here, we evaluated breathing rates and the relative expression of 5HT 2A and 2C receptors in the brainstem as a function of EtOH preexposure in neonates. Pups received i.g administrations of 2.0 or 0.0g/kg EtOH at postnatal days (PD) 3, 5 and 7. At PD 9, breathing frequencies were recorded under normoxia or hypoxia. Brainstems were collected to quantified relative mRNA expression of 5HT 2A and 2C receptors, by qPCR. Under normoxia, EtOH preexposed pups (preEtOH) exhibited high 5HT2A expression levels and breathing depressions. An opposite phenomenon was observed in preEtOH pups tested under hypoxia. An exacerbated hyperventilation associated with low 5HT2A expression levels was found. No significant differences were found in 5HT2C expression levels. These results together with our previous findings that show changes in the raphe obscurus activation patterns, suggest that a brief EtOH preexposure is enough to induce 5HT system?s plasticity, disturbing neonatal breathing. The 5HT components mismatch may be associated with breathing disruptions commonly observed in human neonates, such as Sudden Infant Death Syndrome.Fil: Macchione, Ana Fabiola. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; ArgentinaFil: Anunziata, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Sahonero, Milagros. Universidad Nacional de Córdoba. Facultad de Psicología; ArgentinaFil: Trujillo, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaFil: Molina, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; ArgentinaXXXIV Reunión Anual de la Sociedad Argentina de NeurocienciasVilla Carlos PazArgentinaSociedad Argentina de Investigación en NeurocienciasSAGE Publications2019info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObjectReuniónJournalhttp://purl.org/coar/resource_type/c_5794info:ar-repo/semantics/documentoDeConferenciaapplication/pdfapplication/vnd.openxmlformats-officedocument.wordprocessingml.documentapplication/pdfhttp://hdl.handle.net/11336/159648Early Ethanol Preexposure Modifies Expression of the 5HT2A Receptor Promoting Long-Term Breathing Plasticity in Neonate Rats; XXXIV Reunión Anual de la Sociedad Argentina de Neurociencias; Villa Carlos Paz; Argentina; 2019; 112-1121759-09141759-0914CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1177%2F1759091420979851info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/full/10.1177/1759091420979851Nacionalinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:07:41Zoai:ri.conicet.gov.ar:11336/159648instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:07:41.635CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Early Ethanol Preexposure Modifies Expression of the 5HT2A Receptor Promoting Long-Term Breathing Plasticity in Neonate Rats
title Early Ethanol Preexposure Modifies Expression of the 5HT2A Receptor Promoting Long-Term Breathing Plasticity in Neonate Rats
spellingShingle Early Ethanol Preexposure Modifies Expression of the 5HT2A Receptor Promoting Long-Term Breathing Plasticity in Neonate Rats
Macchione, Ana Fabiola
ETHANOL
BREATHING
5HT2A RECEPTOR
SEROTONIN
BRAINSTEM
title_short Early Ethanol Preexposure Modifies Expression of the 5HT2A Receptor Promoting Long-Term Breathing Plasticity in Neonate Rats
title_full Early Ethanol Preexposure Modifies Expression of the 5HT2A Receptor Promoting Long-Term Breathing Plasticity in Neonate Rats
title_fullStr Early Ethanol Preexposure Modifies Expression of the 5HT2A Receptor Promoting Long-Term Breathing Plasticity in Neonate Rats
title_full_unstemmed Early Ethanol Preexposure Modifies Expression of the 5HT2A Receptor Promoting Long-Term Breathing Plasticity in Neonate Rats
title_sort Early Ethanol Preexposure Modifies Expression of the 5HT2A Receptor Promoting Long-Term Breathing Plasticity in Neonate Rats
dc.creator.none.fl_str_mv Macchione, Ana Fabiola
Anunziata, Florencia
Sahonero, Milagros
Trujillo, Verónica
Molina, Juan Carlos
author Macchione, Ana Fabiola
author_facet Macchione, Ana Fabiola
Anunziata, Florencia
Sahonero, Milagros
Trujillo, Verónica
Molina, Juan Carlos
author_role author
author2 Anunziata, Florencia
Sahonero, Milagros
Trujillo, Verónica
Molina, Juan Carlos
author2_role author
author
author
author
dc.subject.none.fl_str_mv ETHANOL
BREATHING
5HT2A RECEPTOR
SEROTONIN
BRAINSTEM
topic ETHANOL
BREATHING
5HT2A RECEPTOR
SEROTONIN
BRAINSTEM
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv EtOH´s effects upon respiration are attributed to central respiratory network disruptions, especially in the medullary serotonin (5HT) system. 5HT2A/2C receptors are involved in the reduction of the phrenic nerve activity and breathing depression. We hypothesize that early EtOH preexposure alters neonatal respiration through the 5HT system´s plasticity. Here, we evaluated breathing rates and the relative expression of 5HT 2A and 2C receptors in the brainstem as a function of EtOH preexposure in neonates. Pups received i.g administrations of 2.0 or 0.0g/kg EtOH at postnatal days (PD) 3, 5 and 7. At PD 9, breathing frequencies were recorded under normoxia or hypoxia. Brainstems were collected to quantified relative mRNA expression of 5HT 2A and 2C receptors, by qPCR. Under normoxia, EtOH preexposed pups (preEtOH) exhibited high 5HT2A expression levels and breathing depressions. An opposite phenomenon was observed in preEtOH pups tested under hypoxia. An exacerbated hyperventilation associated with low 5HT2A expression levels was found. No significant differences were found in 5HT2C expression levels. These results together with our previous findings that show changes in the raphe obscurus activation patterns, suggest that a brief EtOH preexposure is enough to induce 5HT system?s plasticity, disturbing neonatal breathing. The 5HT components mismatch may be associated with breathing disruptions commonly observed in human neonates, such as Sudden Infant Death Syndrome.
Fil: Macchione, Ana Fabiola. Universidad Nacional de Córdoba. Instituto de Investigaciones Psicológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigaciones Psicológicas; Argentina
Fil: Anunziata, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Sahonero, Milagros. Universidad Nacional de Córdoba. Facultad de Psicología; Argentina
Fil: Trujillo, Verónica. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
Fil: Molina, Juan Carlos. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra. Universidad Nacional de Córdoba. Instituto de Investigación Médica Mercedes y Martín Ferreyra; Argentina
XXXIV Reunión Anual de la Sociedad Argentina de Neurociencias
Villa Carlos Paz
Argentina
Sociedad Argentina de Investigación en Neurociencias
description EtOH´s effects upon respiration are attributed to central respiratory network disruptions, especially in the medullary serotonin (5HT) system. 5HT2A/2C receptors are involved in the reduction of the phrenic nerve activity and breathing depression. We hypothesize that early EtOH preexposure alters neonatal respiration through the 5HT system´s plasticity. Here, we evaluated breathing rates and the relative expression of 5HT 2A and 2C receptors in the brainstem as a function of EtOH preexposure in neonates. Pups received i.g administrations of 2.0 or 0.0g/kg EtOH at postnatal days (PD) 3, 5 and 7. At PD 9, breathing frequencies were recorded under normoxia or hypoxia. Brainstems were collected to quantified relative mRNA expression of 5HT 2A and 2C receptors, by qPCR. Under normoxia, EtOH preexposed pups (preEtOH) exhibited high 5HT2A expression levels and breathing depressions. An opposite phenomenon was observed in preEtOH pups tested under hypoxia. An exacerbated hyperventilation associated with low 5HT2A expression levels was found. No significant differences were found in 5HT2C expression levels. These results together with our previous findings that show changes in the raphe obscurus activation patterns, suggest that a brief EtOH preexposure is enough to induce 5HT system?s plasticity, disturbing neonatal breathing. The 5HT components mismatch may be associated with breathing disruptions commonly observed in human neonates, such as Sudden Infant Death Syndrome.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/conferenceObject
Reunión
Journal
http://purl.org/coar/resource_type/c_5794
info:ar-repo/semantics/documentoDeConferencia
status_str publishedVersion
format conferenceObject
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/159648
Early Ethanol Preexposure Modifies Expression of the 5HT2A Receptor Promoting Long-Term Breathing Plasticity in Neonate Rats; XXXIV Reunión Anual de la Sociedad Argentina de Neurociencias; Villa Carlos Paz; Argentina; 2019; 112-112
1759-0914
1759-0914
CONICET Digital
CONICET
url http://hdl.handle.net/11336/159648
identifier_str_mv Early Ethanol Preexposure Modifies Expression of the 5HT2A Receptor Promoting Long-Term Breathing Plasticity in Neonate Rats; XXXIV Reunión Anual de la Sociedad Argentina de Neurociencias; Villa Carlos Paz; Argentina; 2019; 112-112
1759-0914
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1177%2F1759091420979851
info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/full/10.1177/1759091420979851
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc/2.5/ar/
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rights_invalid_str_mv https://creativecommons.org/licenses/by-nc/2.5/ar/
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application/vnd.openxmlformats-officedocument.wordprocessingml.document
application/pdf
dc.coverage.none.fl_str_mv Nacional
dc.publisher.none.fl_str_mv SAGE Publications
publisher.none.fl_str_mv SAGE Publications
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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