An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle

Autores
Wilkowsky, Silvina Elizabeth; Gimenez Bareiro, Guillermo; Mon, Maria Laura; Moore, Prando Dadin; Caspe, Sergio Gaston; Campero, Carlos Manuel; Fort, Marcelo Cristian; Romano, Maria Isabel
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. Neospora caninum surface antigen 1 (Nc-SAG1) is an immunodominant candidate for the development of a diagnostic reagent for neosporosis. The current study describes the development and evaluation of an antigen print immunoassay (APIA) with recombinant Nc-SAG1 for the detection of specific antibodies to N. caninum in cattle. The concordance between APIA and a commercial enzyme-linked immunosorbent assay (ELISA) was evaluated with 232 serum samples from experimentally and naturally infected cattle. Sixty-one (26.7%) samples were positive for antibodies to N. caninum by ELISA and 58 (25.4%) by APIA. The new assay had a sensitivity of 85% and a specificity of 96%. These results, along with the potential of APIA to evolve into a multiple antigen detection format, suggest that this method would be a reliable diagnostic test for detection of antibodies to N. caninum in cattle.
Instituto de Biotecnología
Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.
Fil: Gimenez Bareiro, Guillermo. Universidad Nacional de Asunción. Faculty of Veterinary Science; Paraguay
Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.
Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Fort, Marcelo Cristian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.
Fuente
Journal of Veterinary Diagnostic Investigation 23 (5) : 971-976. (August 2011)
Materia
Ganado Bovino
Anticuerpos
Inmunoensayos
Diagnóstico
Cattle
Neospora caninum
Antibodies
Immunoassays
Diagnosis
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/14429
Acceder
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oai_identifier_str oai:localhost:20.500.12123/14429
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spelling An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattleWilkowsky, Silvina ElizabethGimenez Bareiro, GuillermoMon, Maria LauraMoore, Prando DadinCaspe, Sergio GastonCampero, Carlos ManuelFort, Marcelo CristianRomano, Maria IsabelGanado BovinoAnticuerposInmunoensayosDiagnósticoCattleNeospora caninumAntibodiesImmunoassaysDiagnosisNeospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. Neospora caninum surface antigen 1 (Nc-SAG1) is an immunodominant candidate for the development of a diagnostic reagent for neosporosis. The current study describes the development and evaluation of an antigen print immunoassay (APIA) with recombinant Nc-SAG1 for the detection of specific antibodies to N. caninum in cattle. The concordance between APIA and a commercial enzyme-linked immunosorbent assay (ELISA) was evaluated with 232 serum samples from experimentally and naturally infected cattle. Sixty-one (26.7%) samples were positive for antibodies to N. caninum by ELISA and 58 (25.4%) by APIA. The new assay had a sensitivity of 85% and a specificity of 96%. These results, along with the potential of APIA to evolve into a multiple antigen detection format, suggest that this method would be a reliable diagnostic test for detection of antibodies to N. caninum in cattle.Instituto de BiotecnologíaFil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.Fil: Gimenez Bareiro, Guillermo. Universidad Nacional de Asunción. Faculty of Veterinary Science; ParaguayFil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; ArgentinaFil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.Fil: Fort, Marcelo Cristian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; ArgentinaFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.Sage Publishing2023-04-10T13:43:31Z2023-04-10T13:43:31Z2011-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/14429https://journals.sagepub.com/doi/10.1177/10406387114168451040-63871943-4936https://doi.org/10.1177/1040638711416845Journal of Veterinary Diagnostic Investigation 23 (5) : 971-976. (August 2011)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:57Zoai:localhost:20.500.12123/14429instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:57.784INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle
title An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle
spellingShingle An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle
Wilkowsky, Silvina Elizabeth
Ganado Bovino
Anticuerpos
Inmunoensayos
Diagnóstico
Cattle
Neospora caninum
Antibodies
Immunoassays
Diagnosis
title_short An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle
title_full An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle
title_fullStr An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle
title_full_unstemmed An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle
title_sort An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to Neospora caninum in cattle
dc.creator.none.fl_str_mv Wilkowsky, Silvina Elizabeth
Gimenez Bareiro, Guillermo
Mon, Maria Laura
Moore, Prando Dadin
Caspe, Sergio Gaston
Campero, Carlos Manuel
Fort, Marcelo Cristian
Romano, Maria Isabel
author Wilkowsky, Silvina Elizabeth
author_facet Wilkowsky, Silvina Elizabeth
Gimenez Bareiro, Guillermo
Mon, Maria Laura
Moore, Prando Dadin
Caspe, Sergio Gaston
Campero, Carlos Manuel
Fort, Marcelo Cristian
Romano, Maria Isabel
author_role author
author2 Gimenez Bareiro, Guillermo
Mon, Maria Laura
Moore, Prando Dadin
Caspe, Sergio Gaston
Campero, Carlos Manuel
Fort, Marcelo Cristian
Romano, Maria Isabel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Anticuerpos
Inmunoensayos
Diagnóstico
Cattle
Neospora caninum
Antibodies
Immunoassays
Diagnosis
topic Ganado Bovino
Anticuerpos
Inmunoensayos
Diagnóstico
Cattle
Neospora caninum
Antibodies
Immunoassays
Diagnosis
dc.description.none.fl_txt_mv Neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. Neospora caninum surface antigen 1 (Nc-SAG1) is an immunodominant candidate for the development of a diagnostic reagent for neosporosis. The current study describes the development and evaluation of an antigen print immunoassay (APIA) with recombinant Nc-SAG1 for the detection of specific antibodies to N. caninum in cattle. The concordance between APIA and a commercial enzyme-linked immunosorbent assay (ELISA) was evaluated with 232 serum samples from experimentally and naturally infected cattle. Sixty-one (26.7%) samples were positive for antibodies to N. caninum by ELISA and 58 (25.4%) by APIA. The new assay had a sensitivity of 85% and a specificity of 96%. These results, along with the potential of APIA to evolve into a multiple antigen detection format, suggest that this method would be a reliable diagnostic test for detection of antibodies to N. caninum in cattle.
Instituto de Biotecnología
Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.
Fil: Gimenez Bareiro, Guillermo. Universidad Nacional de Asunción. Faculty of Veterinary Science; Paraguay
Fil: Mon, Maria Laura. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.
Fil: Moore, Prando Dadin. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Caspe, Sergio Gaston. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Mercedes; Argentina
Fil: Campero, Carlos Manuel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina.
Fil: Fort, Marcelo Cristian. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Anguil; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina.
description Neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. Neospora caninum surface antigen 1 (Nc-SAG1) is an immunodominant candidate for the development of a diagnostic reagent for neosporosis. The current study describes the development and evaluation of an antigen print immunoassay (APIA) with recombinant Nc-SAG1 for the detection of specific antibodies to N. caninum in cattle. The concordance between APIA and a commercial enzyme-linked immunosorbent assay (ELISA) was evaluated with 232 serum samples from experimentally and naturally infected cattle. Sixty-one (26.7%) samples were positive for antibodies to N. caninum by ELISA and 58 (25.4%) by APIA. The new assay had a sensitivity of 85% and a specificity of 96%. These results, along with the potential of APIA to evolve into a multiple antigen detection format, suggest that this method would be a reliable diagnostic test for detection of antibodies to N. caninum in cattle.
publishDate 2011
dc.date.none.fl_str_mv 2011-08
2023-04-10T13:43:31Z
2023-04-10T13:43:31Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/14429
https://journals.sagepub.com/doi/10.1177/1040638711416845
1040-6387
1943-4936
https://doi.org/10.1177/1040638711416845
url http://hdl.handle.net/20.500.12123/14429
https://journals.sagepub.com/doi/10.1177/1040638711416845
https://doi.org/10.1177/1040638711416845
identifier_str_mv 1040-6387
1943-4936
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sage Publishing
publisher.none.fl_str_mv Sage Publishing
dc.source.none.fl_str_mv Journal of Veterinary Diagnostic Investigation 23 (5) : 971-976. (August 2011)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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