Validation and field evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti-Neospora caninum antibodies in sheep and goats

Autores
Novoa, María Belen; Aguirre, Nerina; Ormaechea, Nadia; Palmero, Sebastián; Rouzic, Lisandro; Valentini, Beatriz Susana; Sarli, Macarena; Orcellet, Viviana Mercedes; Marengo, Rafael; Vanzini, Victor Rene; Primo, María Evangelina
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Neospora caninum is a protozoan parasite that causes abortion and reproductive failure in small ruminants. We validated and evaluated under field conditions a competitive inhibition ELISA based on the truncated SAG1 protein (tSAG1) from N. caninum for the detection of anti-N. caninum antibodies in sheep and goat flocks. The assay was validated using 80 positive and 142 negative serum samples from sheep and goats analyzed by IFAT and immunoblot (IB). ciELISAtSAG1 was then used to evaluate the prevalence of anti-N. caninum antibodies in 1449 goats from 143 flocks and 385 sheep from 40 flocks and compared to IFAT. The prevalence of anti-Toxoplasma gondii antibodies was evaluated by IFAT. The ciELISAtSAG1 cut-off was ≥ 36 percent inhibition, with a diagnostic sensitivity of 100.0% (95% CI = 95.4–100.0%) and a diagnostic specificity of 98.6% (95% CI = 95.0–99.8%) relative to the agreement between IFAT and IB. The field evaluation revealed a concordance between ciELISAtSAG1 and IFAT of 97.4%, with an agreement (κ) of 0.90 for sheep sera, and a concordance of 96.5% with κ = 0.85 for goat sera. The overall prevalence of anti-N. caninum antibodies in sheep was 14.3% by IFAT and 15.8% by ciELISAtSAG1. In goats, prevalence was 12.9% by IFAT and 14.6% by ciELISAtSAG1. The overall prevalence of anti-T. gondii antibodies was 28.8% in goats and 43.8% in sheep. The ciELISAtSAG1 could be useful for large-scale detection of anti-N. caninum antibodies in sheep and goats, and for seroepidemiological investigations due to its appropriate sensitivity and specificity, and the simplicity of production.
EEA Rafaela
Fil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Aguirre, Nerina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina.
Fil: Ormaechea, Nadia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Palmero, Sebastián. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Rouzic, Lisandro. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Valentini, Beatriz Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Orcellet, Viviana Mercedes. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Marengo, Rafael. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Vanzini Victor Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Primo, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fuente
Veterinary Parasitology : 109201 (Available online 7 August 2020)
Materia
Ovinos
Caprinos
Enfermedades de los Animales
Neospora caninum
Diagnóstico
ELISA
Toxoplasma gondii
Anticuerpos
Sheep
Goats
Animal Diseases
Diagnosis
Antibodies
Nivel de accesibilidad
acceso abierto
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7699
Acceder
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oai_identifier_str oai:localhost:20.500.12123/7699
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Validation and field evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti-Neospora caninum antibodies in sheep and goatsNovoa, María BelenAguirre, NerinaOrmaechea, NadiaPalmero, SebastiánRouzic, LisandroValentini, Beatriz SusanaSarli, MacarenaOrcellet, Viviana MercedesMarengo, RafaelVanzini, Victor RenePrimo, María EvangelinaOvinosCaprinosEnfermedades de los AnimalesNeospora caninumDiagnósticoELISAToxoplasma gondiiAnticuerposSheepGoatsAnimal DiseasesDiagnosisAntibodiesNeospora caninum is a protozoan parasite that causes abortion and reproductive failure in small ruminants. We validated and evaluated under field conditions a competitive inhibition ELISA based on the truncated SAG1 protein (tSAG1) from N. caninum for the detection of anti-N. caninum antibodies in sheep and goat flocks. The assay was validated using 80 positive and 142 negative serum samples from sheep and goats analyzed by IFAT and immunoblot (IB). ciELISAtSAG1 was then used to evaluate the prevalence of anti-N. caninum antibodies in 1449 goats from 143 flocks and 385 sheep from 40 flocks and compared to IFAT. The prevalence of anti-Toxoplasma gondii antibodies was evaluated by IFAT. The ciELISAtSAG1 cut-off was ≥ 36 percent inhibition, with a diagnostic sensitivity of 100.0% (95% CI = 95.4–100.0%) and a diagnostic specificity of 98.6% (95% CI = 95.0–99.8%) relative to the agreement between IFAT and IB. The field evaluation revealed a concordance between ciELISAtSAG1 and IFAT of 97.4%, with an agreement (κ) of 0.90 for sheep sera, and a concordance of 96.5% with κ = 0.85 for goat sera. The overall prevalence of anti-N. caninum antibodies in sheep was 14.3% by IFAT and 15.8% by ciELISAtSAG1. In goats, prevalence was 12.9% by IFAT and 14.6% by ciELISAtSAG1. The overall prevalence of anti-T. gondii antibodies was 28.8% in goats and 43.8% in sheep. The ciELISAtSAG1 could be useful for large-scale detection of anti-N. caninum antibodies in sheep and goats, and for seroepidemiological investigations due to its appropriate sensitivity and specificity, and the simplicity of production.EEA RafaelaFil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Aguirre, Nerina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina.Fil: Ormaechea, Nadia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Palmero, Sebastián. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Rouzic, Lisandro. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Valentini, Beatriz Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Orcellet, Viviana Mercedes. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Marengo, Rafael. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Vanzini Victor Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; ArgentinaFil: Primo, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Elsevierinfo:eu-repo/date/embargoEnd/2021-08-112020-08-11T11:54:42Z2020-08-11T11:54:42Z2020-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7699https://www.sciencedirect.com/science/article/abs/pii/S03044017203018130304-4017https://doi.org/10.1016/j.vetpar.2020.109201Veterinary Parasitology : 109201 (Available online 7 August 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccess2025-09-18T10:07:58Zoai:localhost:20.500.12123/7699instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-18 10:07:58.984INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Validation and field evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti-Neospora caninum antibodies in sheep and goats
title Validation and field evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti-Neospora caninum antibodies in sheep and goats
spellingShingle Validation and field evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti-Neospora caninum antibodies in sheep and goats
Novoa, María Belen
Ovinos
Caprinos
Enfermedades de los Animales
Neospora caninum
Diagnóstico
ELISA
Toxoplasma gondii
Anticuerpos
Sheep
Goats
Animal Diseases
Diagnosis
Antibodies
title_short Validation and field evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti-Neospora caninum antibodies in sheep and goats
title_full Validation and field evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti-Neospora caninum antibodies in sheep and goats
title_fullStr Validation and field evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti-Neospora caninum antibodies in sheep and goats
title_full_unstemmed Validation and field evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti-Neospora caninum antibodies in sheep and goats
title_sort Validation and field evaluation of a competitive inhibition ELISA based on the recombinant protein tSAG1 to detect anti-Neospora caninum antibodies in sheep and goats
dc.creator.none.fl_str_mv Novoa, María Belen
Aguirre, Nerina
Ormaechea, Nadia
Palmero, Sebastián
Rouzic, Lisandro
Valentini, Beatriz Susana
Sarli, Macarena
Orcellet, Viviana Mercedes
Marengo, Rafael
Vanzini, Victor Rene
Primo, María Evangelina
author Novoa, María Belen
author_facet Novoa, María Belen
Aguirre, Nerina
Ormaechea, Nadia
Palmero, Sebastián
Rouzic, Lisandro
Valentini, Beatriz Susana
Sarli, Macarena
Orcellet, Viviana Mercedes
Marengo, Rafael
Vanzini, Victor Rene
Primo, María Evangelina
author_role author
author2 Aguirre, Nerina
Ormaechea, Nadia
Palmero, Sebastián
Rouzic, Lisandro
Valentini, Beatriz Susana
Sarli, Macarena
Orcellet, Viviana Mercedes
Marengo, Rafael
Vanzini, Victor Rene
Primo, María Evangelina
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ovinos
Caprinos
Enfermedades de los Animales
Neospora caninum
Diagnóstico
ELISA
Toxoplasma gondii
Anticuerpos
Sheep
Goats
Animal Diseases
Diagnosis
Antibodies
topic Ovinos
Caprinos
Enfermedades de los Animales
Neospora caninum
Diagnóstico
ELISA
Toxoplasma gondii
Anticuerpos
Sheep
Goats
Animal Diseases
Diagnosis
Antibodies
dc.description.none.fl_txt_mv Neospora caninum is a protozoan parasite that causes abortion and reproductive failure in small ruminants. We validated and evaluated under field conditions a competitive inhibition ELISA based on the truncated SAG1 protein (tSAG1) from N. caninum for the detection of anti-N. caninum antibodies in sheep and goat flocks. The assay was validated using 80 positive and 142 negative serum samples from sheep and goats analyzed by IFAT and immunoblot (IB). ciELISAtSAG1 was then used to evaluate the prevalence of anti-N. caninum antibodies in 1449 goats from 143 flocks and 385 sheep from 40 flocks and compared to IFAT. The prevalence of anti-Toxoplasma gondii antibodies was evaluated by IFAT. The ciELISAtSAG1 cut-off was ≥ 36 percent inhibition, with a diagnostic sensitivity of 100.0% (95% CI = 95.4–100.0%) and a diagnostic specificity of 98.6% (95% CI = 95.0–99.8%) relative to the agreement between IFAT and IB. The field evaluation revealed a concordance between ciELISAtSAG1 and IFAT of 97.4%, with an agreement (κ) of 0.90 for sheep sera, and a concordance of 96.5% with κ = 0.85 for goat sera. The overall prevalence of anti-N. caninum antibodies in sheep was 14.3% by IFAT and 15.8% by ciELISAtSAG1. In goats, prevalence was 12.9% by IFAT and 14.6% by ciELISAtSAG1. The overall prevalence of anti-T. gondii antibodies was 28.8% in goats and 43.8% in sheep. The ciELISAtSAG1 could be useful for large-scale detection of anti-N. caninum antibodies in sheep and goats, and for seroepidemiological investigations due to its appropriate sensitivity and specificity, and the simplicity of production.
EEA Rafaela
Fil: Novoa, María Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Aguirre, Nerina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina.
Fil: Ormaechea, Nadia. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Palmero, Sebastián. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Rouzic, Lisandro. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Valentini, Beatriz Susana. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Sarli, Macarena. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
Fil: Orcellet, Viviana Mercedes. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Marengo, Rafael. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Vanzini Victor Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina
Fil: Primo, María Evangelina. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.
description Neospora caninum is a protozoan parasite that causes abortion and reproductive failure in small ruminants. We validated and evaluated under field conditions a competitive inhibition ELISA based on the truncated SAG1 protein (tSAG1) from N. caninum for the detection of anti-N. caninum antibodies in sheep and goat flocks. The assay was validated using 80 positive and 142 negative serum samples from sheep and goats analyzed by IFAT and immunoblot (IB). ciELISAtSAG1 was then used to evaluate the prevalence of anti-N. caninum antibodies in 1449 goats from 143 flocks and 385 sheep from 40 flocks and compared to IFAT. The prevalence of anti-Toxoplasma gondii antibodies was evaluated by IFAT. The ciELISAtSAG1 cut-off was ≥ 36 percent inhibition, with a diagnostic sensitivity of 100.0% (95% CI = 95.4–100.0%) and a diagnostic specificity of 98.6% (95% CI = 95.0–99.8%) relative to the agreement between IFAT and IB. The field evaluation revealed a concordance between ciELISAtSAG1 and IFAT of 97.4%, with an agreement (κ) of 0.90 for sheep sera, and a concordance of 96.5% with κ = 0.85 for goat sera. The overall prevalence of anti-N. caninum antibodies in sheep was 14.3% by IFAT and 15.8% by ciELISAtSAG1. In goats, prevalence was 12.9% by IFAT and 14.6% by ciELISAtSAG1. The overall prevalence of anti-T. gondii antibodies was 28.8% in goats and 43.8% in sheep. The ciELISAtSAG1 could be useful for large-scale detection of anti-N. caninum antibodies in sheep and goats, and for seroepidemiological investigations due to its appropriate sensitivity and specificity, and the simplicity of production.
publishDate 2020
dc.date.none.fl_str_mv 2020-08-11T11:54:42Z
2020-08-11T11:54:42Z
2020-08
info:eu-repo/date/embargoEnd/2021-08-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7699
https://www.sciencedirect.com/science/article/abs/pii/S0304401720301813
0304-4017
https://doi.org/10.1016/j.vetpar.2020.109201
url http://hdl.handle.net/20.500.12123/7699
https://www.sciencedirect.com/science/article/abs/pii/S0304401720301813
https://doi.org/10.1016/j.vetpar.2020.109201
identifier_str_mv 0304-4017
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Veterinary Parasitology : 109201 (Available online 7 August 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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