Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera

Autores
Mansilla, Florencia Celeste; Gual, Ignacio; Pereyra, Walter Rodrigo; Cardoso, Nancy Patricia; Turco, Cecilia; Capozzo, Alejandra Victoria
Año de publicación
2019
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of the major causes of reproductive losses in cattle affecting both dairy and beef industries. Several techniques are regularly used for the diagnosis of bovine neosporosis. Amongst them, serological techniques are generally preferred, being IFAT the traditionally accepted reference method for diagnosis (gold standard). However, ELISA has become the most eligible method for large-scale screening of specific antibodies against N. caninum in diagnostic laboratories around the world, providing results that can be easily standardized between laboratories, within a few hours at a relative low-cost. There is no local production of commercial ELISA tests nor in Argentina neither in South America, therefore they must be purchased abroad, turning unfeasible the routinely use of ELISA tests. Here we have developed and validated accordingly to OIE specifications a novel ELISA test based on soluble antigens from N. caninum tachyzoites to accurately determine the presence of specific antibodies anti- N. caninum antigens in bovine sera samples, enabling the rapid, objective and low-cost diagnosis of bovine neosporosis in Argentina; replacing IFAT and avoiding delays and high importation taxes.
EEA Balcarce
Fil: Mansilla Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.
Fil: Gual Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pereyra Walter Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cardoso Nancy. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Turco Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.
Fil: Capozzo Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Journal of veterinary science and animal husbandry 7 (2) : 204. (August 29, 2019)
Materia
Técnicas Inmunológicas
ELISA
Bovinae
Neospora Caninum
Anticuerpos
Immunological Techniques
Bovina
Antibodies
IgG
Bovine Sera Samples
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7466
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spelling Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine seraMansilla, Florencia CelesteGual, IgnacioPereyra, Walter RodrigoCardoso, Nancy PatriciaTurco, CeciliaCapozzo, Alejandra VictoriaTécnicas InmunológicasELISABovinaeNeospora CaninumAnticuerposImmunological TechniquesBovinaAntibodiesIgGBovine Sera SamplesNeospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of the major causes of reproductive losses in cattle affecting both dairy and beef industries. Several techniques are regularly used for the diagnosis of bovine neosporosis. Amongst them, serological techniques are generally preferred, being IFAT the traditionally accepted reference method for diagnosis (gold standard). However, ELISA has become the most eligible method for large-scale screening of specific antibodies against N. caninum in diagnostic laboratories around the world, providing results that can be easily standardized between laboratories, within a few hours at a relative low-cost. There is no local production of commercial ELISA tests nor in Argentina neither in South America, therefore they must be purchased abroad, turning unfeasible the routinely use of ELISA tests. Here we have developed and validated accordingly to OIE specifications a novel ELISA test based on soluble antigens from N. caninum tachyzoites to accurately determine the presence of specific antibodies anti- N. caninum antigens in bovine sera samples, enabling the rapid, objective and low-cost diagnosis of bovine neosporosis in Argentina; replacing IFAT and avoiding delays and high importation taxes.EEA BalcarceFil: Mansilla Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.Fil: Gual Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Pereyra Walter Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Cardoso Nancy. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Turco Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.Fil: Capozzo Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAnnex Publishers2020-06-24T15:24:58Z2020-06-24T15:24:58Z2019-08-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://www.annexpublishers.com/articles/JVSAH/7204-Development-and-Validation-of-a-Novel-ELISA-for-the-Detection-of-Neospora-Caninum-Antibodies-in-Bovine-Sera.pdfhttp://hdl.handle.net/20.500.12123/74662348-9790Journal of veterinary science and animal husbandry 7 (2) : 204. (August 29, 2019)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:50Zoai:localhost:20.500.12123/7466instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:50.63INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera
title Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera
spellingShingle Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera
Mansilla, Florencia Celeste
Técnicas Inmunológicas
ELISA
Bovinae
Neospora Caninum
Anticuerpos
Immunological Techniques
Bovina
Antibodies
IgG
Bovine Sera Samples
title_short Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera
title_full Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera
title_fullStr Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera
title_full_unstemmed Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera
title_sort Development and validation of a novel ELISA for the detection of Neospora caninum antibodies in bovine sera
dc.creator.none.fl_str_mv Mansilla, Florencia Celeste
Gual, Ignacio
Pereyra, Walter Rodrigo
Cardoso, Nancy Patricia
Turco, Cecilia
Capozzo, Alejandra Victoria
author Mansilla, Florencia Celeste
author_facet Mansilla, Florencia Celeste
Gual, Ignacio
Pereyra, Walter Rodrigo
Cardoso, Nancy Patricia
Turco, Cecilia
Capozzo, Alejandra Victoria
author_role author
author2 Gual, Ignacio
Pereyra, Walter Rodrigo
Cardoso, Nancy Patricia
Turco, Cecilia
Capozzo, Alejandra Victoria
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Técnicas Inmunológicas
ELISA
Bovinae
Neospora Caninum
Anticuerpos
Immunological Techniques
Bovina
Antibodies
IgG
Bovine Sera Samples
topic Técnicas Inmunológicas
ELISA
Bovinae
Neospora Caninum
Anticuerpos
Immunological Techniques
Bovina
Antibodies
IgG
Bovine Sera Samples
dc.description.none.fl_txt_mv Neospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of the major causes of reproductive losses in cattle affecting both dairy and beef industries. Several techniques are regularly used for the diagnosis of bovine neosporosis. Amongst them, serological techniques are generally preferred, being IFAT the traditionally accepted reference method for diagnosis (gold standard). However, ELISA has become the most eligible method for large-scale screening of specific antibodies against N. caninum in diagnostic laboratories around the world, providing results that can be easily standardized between laboratories, within a few hours at a relative low-cost. There is no local production of commercial ELISA tests nor in Argentina neither in South America, therefore they must be purchased abroad, turning unfeasible the routinely use of ELISA tests. Here we have developed and validated accordingly to OIE specifications a novel ELISA test based on soluble antigens from N. caninum tachyzoites to accurately determine the presence of specific antibodies anti- N. caninum antigens in bovine sera samples, enabling the rapid, objective and low-cost diagnosis of bovine neosporosis in Argentina; replacing IFAT and avoiding delays and high importation taxes.
EEA Balcarce
Fil: Mansilla Florencia Celeste. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.
Fil: Gual Ignacio. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Pereyra Walter Rodrigo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Salta; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Cardoso Nancy. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Turco Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.
Fil: Capozzo Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Neospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of the major causes of reproductive losses in cattle affecting both dairy and beef industries. Several techniques are regularly used for the diagnosis of bovine neosporosis. Amongst them, serological techniques are generally preferred, being IFAT the traditionally accepted reference method for diagnosis (gold standard). However, ELISA has become the most eligible method for large-scale screening of specific antibodies against N. caninum in diagnostic laboratories around the world, providing results that can be easily standardized between laboratories, within a few hours at a relative low-cost. There is no local production of commercial ELISA tests nor in Argentina neither in South America, therefore they must be purchased abroad, turning unfeasible the routinely use of ELISA tests. Here we have developed and validated accordingly to OIE specifications a novel ELISA test based on soluble antigens from N. caninum tachyzoites to accurately determine the presence of specific antibodies anti- N. caninum antigens in bovine sera samples, enabling the rapid, objective and low-cost diagnosis of bovine neosporosis in Argentina; replacing IFAT and avoiding delays and high importation taxes.
publishDate 2019
dc.date.none.fl_str_mv 2019-08-26
2020-06-24T15:24:58Z
2020-06-24T15:24:58Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://www.annexpublishers.com/articles/JVSAH/7204-Development-and-Validation-of-a-Novel-ELISA-for-the-Detection-of-Neospora-Caninum-Antibodies-in-Bovine-Sera.pdf
http://hdl.handle.net/20.500.12123/7466
2348-9790
url http://www.annexpublishers.com/articles/JVSAH/7204-Development-and-Validation-of-a-Novel-ELISA-for-the-Detection-of-Neospora-Caninum-Antibodies-in-Bovine-Sera.pdf
http://hdl.handle.net/20.500.12123/7466
identifier_str_mv 2348-9790
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Annex Publishers
publisher.none.fl_str_mv Annex Publishers
dc.source.none.fl_str_mv Journal of veterinary science and animal husbandry 7 (2) : 204. (August 29, 2019)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.6140175

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