An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to neospora caninum in cattle

Autores
Wilkowsky, Silvina Elizabeth; Gimenez Bareiro, Guillermo; Mon, Maria Laura; Moore, Dadin Prando; Caspe, Gastón; Campero, Carlos; Fort, Marcelo; Romano, Maria Isabel
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. Neospora caninum surface antigen 1 (Nc-SAG1) is an immunodominant candidate for the development of a diagnostic reagent for neosporosis. The current study describes the development and evaluation of an antigen print immunoassay (APIA) with recombinant Nc-SAG1 for the detection of specific antibodies to N. caninum in cattle. The concordance between APIA and a commercial enzyme-linked immunosorbent assay (ELISA) was evaluated with 232 serum samples from experimentally and naturally infected cattle. Sixty-one (26.7%) samples were positive for antibodies to N. caninum by ELISA and 58 (25.4%) by APIA. The new assay had a sensitivity of 85% and a specificity of 96%. These results, along with the potential of APIA to evolve into a multiple antigen detection format, suggest that this method would be a reliable diagnostic test for detection of antibodies to N. caninum in cattle.
Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gimenez Bareiro, Guillermo. Universidad Nacional de Asunción; Paraguay
Fil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar; Argentina
Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caspe, Gastón. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Campero, Carlos. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Fort, Marcelo. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Materia
APPLIED PRINTING IMMUNOASSAY
DIAGNOSIS
NC-SAG1
NEOSPORA CANINUM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/199018
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/199018
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to neospora caninum in cattleWilkowsky, Silvina ElizabethGimenez Bareiro, GuillermoMon, Maria LauraMoore, Dadin PrandoCaspe, GastónCampero, CarlosFort, MarceloRomano, Maria IsabelAPPLIED PRINTING IMMUNOASSAYDIAGNOSISNC-SAG1NEOSPORA CANINUMhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4https://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. Neospora caninum surface antigen 1 (Nc-SAG1) is an immunodominant candidate for the development of a diagnostic reagent for neosporosis. The current study describes the development and evaluation of an antigen print immunoassay (APIA) with recombinant Nc-SAG1 for the detection of specific antibodies to N. caninum in cattle. The concordance between APIA and a commercial enzyme-linked immunosorbent assay (ELISA) was evaluated with 232 serum samples from experimentally and naturally infected cattle. Sixty-one (26.7%) samples were positive for antibodies to N. caninum by ELISA and 58 (25.4%) by APIA. The new assay had a sensitivity of 85% and a specificity of 96%. These results, along with the potential of APIA to evolve into a multiple antigen detection format, suggest that this method would be a reliable diagnostic test for detection of antibodies to N. caninum in cattle.Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Gimenez Bareiro, Guillermo. Universidad Nacional de Asunción; ParaguayFil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar; ArgentinaFil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Caspe, Gastón. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Campero, Carlos. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Fort, Marcelo. Instituto Nacional de Tecnología Agropecuaria; ArgentinaFil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaAmerican Association of Veterinary Laboratory Diagnosticians2011-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/199018Wilkowsky, Silvina Elizabeth; Gimenez Bareiro, Guillermo; Mon, Maria Laura; Moore, Dadin Prando; Caspe, Gastón; et al.; An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to neospora caninum in cattle; American Association of Veterinary Laboratory Diagnosticians; Journal of Veterinary Diagnostic Investigation; 23; 5; 9-2011; 971-9761040-6387CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1040638711416845info:eu-repo/semantics/altIdentifier/doi/10.1177/1040638711416845info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:46:15Zoai:ri.conicet.gov.ar:11336/199018instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:46:15.88CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to neospora caninum in cattle
title An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to neospora caninum in cattle
spellingShingle An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to neospora caninum in cattle
Wilkowsky, Silvina Elizabeth
APPLIED PRINTING IMMUNOASSAY
DIAGNOSIS
NC-SAG1
NEOSPORA CANINUM
title_short An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to neospora caninum in cattle
title_full An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to neospora caninum in cattle
title_fullStr An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to neospora caninum in cattle
title_full_unstemmed An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to neospora caninum in cattle
title_sort An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to neospora caninum in cattle
dc.creator.none.fl_str_mv Wilkowsky, Silvina Elizabeth
Gimenez Bareiro, Guillermo
Mon, Maria Laura
Moore, Dadin Prando
Caspe, Gastón
Campero, Carlos
Fort, Marcelo
Romano, Maria Isabel
author Wilkowsky, Silvina Elizabeth
author_facet Wilkowsky, Silvina Elizabeth
Gimenez Bareiro, Guillermo
Mon, Maria Laura
Moore, Dadin Prando
Caspe, Gastón
Campero, Carlos
Fort, Marcelo
Romano, Maria Isabel
author_role author
author2 Gimenez Bareiro, Guillermo
Mon, Maria Laura
Moore, Dadin Prando
Caspe, Gastón
Campero, Carlos
Fort, Marcelo
Romano, Maria Isabel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv APPLIED PRINTING IMMUNOASSAY
DIAGNOSIS
NC-SAG1
NEOSPORA CANINUM
topic APPLIED PRINTING IMMUNOASSAY
DIAGNOSIS
NC-SAG1
NEOSPORA CANINUM
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. Neospora caninum surface antigen 1 (Nc-SAG1) is an immunodominant candidate for the development of a diagnostic reagent for neosporosis. The current study describes the development and evaluation of an antigen print immunoassay (APIA) with recombinant Nc-SAG1 for the detection of specific antibodies to N. caninum in cattle. The concordance between APIA and a commercial enzyme-linked immunosorbent assay (ELISA) was evaluated with 232 serum samples from experimentally and naturally infected cattle. Sixty-one (26.7%) samples were positive for antibodies to N. caninum by ELISA and 58 (25.4%) by APIA. The new assay had a sensitivity of 85% and a specificity of 96%. These results, along with the potential of APIA to evolve into a multiple antigen detection format, suggest that this method would be a reliable diagnostic test for detection of antibodies to N. caninum in cattle.
Fil: Wilkowsky, Silvina Elizabeth. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Agrobiotecnología y Biología Molecular. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Gimenez Bareiro, Guillermo. Universidad Nacional de Asunción; Paraguay
Fil: Mon, Maria Laura. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar; Argentina
Fil: Moore, Dadin Prando. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce. Agencia de Extensión Rural Balcarce; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caspe, Gastón. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Campero, Carlos. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Fort, Marcelo. Instituto Nacional de Tecnología Agropecuaria; Argentina
Fil: Romano, Maria Isabel. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Neospora caninum is a protozoan parasite that causes an important reproductive disease in cattle. Neospora caninum surface antigen 1 (Nc-SAG1) is an immunodominant candidate for the development of a diagnostic reagent for neosporosis. The current study describes the development and evaluation of an antigen print immunoassay (APIA) with recombinant Nc-SAG1 for the detection of specific antibodies to N. caninum in cattle. The concordance between APIA and a commercial enzyme-linked immunosorbent assay (ELISA) was evaluated with 232 serum samples from experimentally and naturally infected cattle. Sixty-one (26.7%) samples were positive for antibodies to N. caninum by ELISA and 58 (25.4%) by APIA. The new assay had a sensitivity of 85% and a specificity of 96%. These results, along with the potential of APIA to evolve into a multiple antigen detection format, suggest that this method would be a reliable diagnostic test for detection of antibodies to N. caninum in cattle.
publishDate 2011
dc.date.none.fl_str_mv 2011-09
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/199018
Wilkowsky, Silvina Elizabeth; Gimenez Bareiro, Guillermo; Mon, Maria Laura; Moore, Dadin Prando; Caspe, Gastón; et al.; An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to neospora caninum in cattle; American Association of Veterinary Laboratory Diagnosticians; Journal of Veterinary Diagnostic Investigation; 23; 5; 9-2011; 971-976
1040-6387
CONICET Digital
CONICET
url http://hdl.handle.net/11336/199018
identifier_str_mv Wilkowsky, Silvina Elizabeth; Gimenez Bareiro, Guillermo; Mon, Maria Laura; Moore, Dadin Prando; Caspe, Gastón; et al.; An applied printing immunoassay with recombinant Nc-SAG1 for detection of antibodies to neospora caninum in cattle; American Association of Veterinary Laboratory Diagnosticians; Journal of Veterinary Diagnostic Investigation; 23; 5; 9-2011; 971-976
1040-6387
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://journals.sagepub.com/doi/10.1177/1040638711416845
info:eu-repo/semantics/altIdentifier/doi/10.1177/1040638711416845
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv American Association of Veterinary Laboratory Diagnosticians
publisher.none.fl_str_mv American Association of Veterinary Laboratory Diagnosticians
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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