Development and Validation of a Novel ELISA for the Detection of Neospora Caninum Antibodies in Bovine Sera

Autores
Mansilla, Florencia Celeste; Gual, Ignacio; Pereyra, Rodrigo; Turco, Cecilia Soledad; Capozzo, Alejandra Victoria
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Neospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of themajor causes of reproductive losses in cattle affecting both dairy and beef industries. Several techniques are regularly used for the diagnosis of bovine neosporosis. Amongst them, serological techniques are generally preferred, being IFAT the traditionally accepted reference method for diagnosis (gold standard). However, ELISA has become the most eligible method for large-scale screening ofspecific antibodies against N. caninum in diagnostic laboratories around the world, providing results that can be easily standardized between laboratories, within a few hours at a relative low-cost. There is no local production of commercial ELISA tests nor in Argentina neither in South America, therefore they must be purchased abroad, turning unfeasible the routinely use of ELISA tests. Here we have developed and validated accordingly to OIE specifications a novel ELISA test based on soluble antigens from N. caninum tachyzoites to accurately determine the presence of specific antibodies anti- N. caninum antigens in bovine sera samples, enabling the rapid, objective and low-cost diagnosis of bovine neosporosis in Argentina; replacing IFAT and avoiding delays and high importation taxes.
Fil: Mansilla, Florencia Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Gual, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Pereyra, Rodrigo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Turco, Cecilia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
Materia
NEOSPORA CANINUM
ELISA
BOVINE SERA
ANTIBODIES
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/112293
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/112293
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Development and Validation of a Novel ELISA for the Detection of Neospora Caninum Antibodies in Bovine SeraMansilla, Florencia CelesteGual, IgnacioPereyra, RodrigoTurco, Cecilia SoledadCapozzo, Alejandra VictoriaNEOSPORA CANINUMELISABOVINE SERAANTIBODIEShttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Neospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of themajor causes of reproductive losses in cattle affecting both dairy and beef industries. Several techniques are regularly used for the diagnosis of bovine neosporosis. Amongst them, serological techniques are generally preferred, being IFAT the traditionally accepted reference method for diagnosis (gold standard). However, ELISA has become the most eligible method for large-scale screening ofspecific antibodies against N. caninum in diagnostic laboratories around the world, providing results that can be easily standardized between laboratories, within a few hours at a relative low-cost. There is no local production of commercial ELISA tests nor in Argentina neither in South America, therefore they must be purchased abroad, turning unfeasible the routinely use of ELISA tests. Here we have developed and validated accordingly to OIE specifications a novel ELISA test based on soluble antigens from N. caninum tachyzoites to accurately determine the presence of specific antibodies anti- N. caninum antigens in bovine sera samples, enabling the rapid, objective and low-cost diagnosis of bovine neosporosis in Argentina; replacing IFAT and avoiding delays and high importation taxes.Fil: Mansilla, Florencia Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; ArgentinaFil: Gual, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; ArgentinaFil: Pereyra, Rodrigo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; ArgentinaFil: Turco, Cecilia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; ArgentinaFil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; ArgentinaAnnex Publishers2020-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/112293Mansilla, Florencia Celeste; Gual, Ignacio; Pereyra, Rodrigo; Turco, Cecilia Soledad; Capozzo, Alejandra Victoria; Development and Validation of a Novel ELISA for the Detection of Neospora Caninum Antibodies in Bovine Sera; Annex Publishers; Journal of Veterinary Science & Animal Husbandry; 7; 2; 4-2020; 1-102348-9790CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/http://www.annexpublishers.com/articles/JVSAH/7204-Development-and-Validation-of-a-Novel-ELISA-for-the-Detection-of-Neospora-Caninum-Antibodies-in-Bovine-Sera.pdfinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-17T11:46:21Zoai:ri.conicet.gov.ar:11336/112293instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-17 11:46:21.488CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Development and Validation of a Novel ELISA for the Detection of Neospora Caninum Antibodies in Bovine Sera
title Development and Validation of a Novel ELISA for the Detection of Neospora Caninum Antibodies in Bovine Sera
spellingShingle Development and Validation of a Novel ELISA for the Detection of Neospora Caninum Antibodies in Bovine Sera
Mansilla, Florencia Celeste
NEOSPORA CANINUM
ELISA
BOVINE SERA
ANTIBODIES
title_short Development and Validation of a Novel ELISA for the Detection of Neospora Caninum Antibodies in Bovine Sera
title_full Development and Validation of a Novel ELISA for the Detection of Neospora Caninum Antibodies in Bovine Sera
title_fullStr Development and Validation of a Novel ELISA for the Detection of Neospora Caninum Antibodies in Bovine Sera
title_full_unstemmed Development and Validation of a Novel ELISA for the Detection of Neospora Caninum Antibodies in Bovine Sera
title_sort Development and Validation of a Novel ELISA for the Detection of Neospora Caninum Antibodies in Bovine Sera
dc.creator.none.fl_str_mv Mansilla, Florencia Celeste
Gual, Ignacio
Pereyra, Rodrigo
Turco, Cecilia Soledad
Capozzo, Alejandra Victoria
author Mansilla, Florencia Celeste
author_facet Mansilla, Florencia Celeste
Gual, Ignacio
Pereyra, Rodrigo
Turco, Cecilia Soledad
Capozzo, Alejandra Victoria
author_role author
author2 Gual, Ignacio
Pereyra, Rodrigo
Turco, Cecilia Soledad
Capozzo, Alejandra Victoria
author2_role author
author
author
author
dc.subject.none.fl_str_mv NEOSPORA CANINUM
ELISA
BOVINE SERA
ANTIBODIES
topic NEOSPORA CANINUM
ELISA
BOVINE SERA
ANTIBODIES
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Neospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of themajor causes of reproductive losses in cattle affecting both dairy and beef industries. Several techniques are regularly used for the diagnosis of bovine neosporosis. Amongst them, serological techniques are generally preferred, being IFAT the traditionally accepted reference method for diagnosis (gold standard). However, ELISA has become the most eligible method for large-scale screening ofspecific antibodies against N. caninum in diagnostic laboratories around the world, providing results that can be easily standardized between laboratories, within a few hours at a relative low-cost. There is no local production of commercial ELISA tests nor in Argentina neither in South America, therefore they must be purchased abroad, turning unfeasible the routinely use of ELISA tests. Here we have developed and validated accordingly to OIE specifications a novel ELISA test based on soluble antigens from N. caninum tachyzoites to accurately determine the presence of specific antibodies anti- N. caninum antigens in bovine sera samples, enabling the rapid, objective and low-cost diagnosis of bovine neosporosis in Argentina; replacing IFAT and avoiding delays and high importation taxes.
Fil: Mansilla, Florencia Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Gual, Ignacio. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Buenos Aires Sur. Estación Experimental Agropecuaria Balcarce; Argentina
Fil: Pereyra, Rodrigo. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Turco, Cecilia Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina
Fil: Capozzo, Alejandra Victoria. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario; Argentina
description Neospora caninum is a world-wide distributed apicomplexan parasite, causative agent of bovine neosporosis, which is one of themajor causes of reproductive losses in cattle affecting both dairy and beef industries. Several techniques are regularly used for the diagnosis of bovine neosporosis. Amongst them, serological techniques are generally preferred, being IFAT the traditionally accepted reference method for diagnosis (gold standard). However, ELISA has become the most eligible method for large-scale screening ofspecific antibodies against N. caninum in diagnostic laboratories around the world, providing results that can be easily standardized between laboratories, within a few hours at a relative low-cost. There is no local production of commercial ELISA tests nor in Argentina neither in South America, therefore they must be purchased abroad, turning unfeasible the routinely use of ELISA tests. Here we have developed and validated accordingly to OIE specifications a novel ELISA test based on soluble antigens from N. caninum tachyzoites to accurately determine the presence of specific antibodies anti- N. caninum antigens in bovine sera samples, enabling the rapid, objective and low-cost diagnosis of bovine neosporosis in Argentina; replacing IFAT and avoiding delays and high importation taxes.
publishDate 2020
dc.date.none.fl_str_mv 2020-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/112293
Mansilla, Florencia Celeste; Gual, Ignacio; Pereyra, Rodrigo; Turco, Cecilia Soledad; Capozzo, Alejandra Victoria; Development and Validation of a Novel ELISA for the Detection of Neospora Caninum Antibodies in Bovine Sera; Annex Publishers; Journal of Veterinary Science & Animal Husbandry; 7; 2; 4-2020; 1-10
2348-9790
CONICET Digital
CONICET
url http://hdl.handle.net/11336/112293
identifier_str_mv Mansilla, Florencia Celeste; Gual, Ignacio; Pereyra, Rodrigo; Turco, Cecilia Soledad; Capozzo, Alejandra Victoria; Development and Validation of a Novel ELISA for the Detection of Neospora Caninum Antibodies in Bovine Sera; Annex Publishers; Journal of Veterinary Science & Animal Husbandry; 7; 2; 4-2020; 1-10
2348-9790
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/http://www.annexpublishers.com/articles/JVSAH/7204-Development-and-Validation-of-a-Novel-ELISA-for-the-Detection-of-Neospora-Caninum-Antibodies-in-Bovine-Sera.pdf
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Annex Publishers
publisher.none.fl_str_mv Annex Publishers
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1843606814593122304
score 13.001348

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