Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus

Autores
Kopertekh, Lilya; Krebs, Erika; Guzman, Fabiana
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To study the impact of regulatory sequences from Cowpea mosaic virus (CPMV) on Cre-mediated recombination rates, the cre gene was flanked by the 5′ non-translated and 3′ non-translated regions of CPMV. This cre configuration was tested by simultaneous excision of nptII selectable marker gene and heat-inducible cre recombinase gene in potato. Fusion of the cre recombinase sequence with modified regulatory sequences of CPMV increased both the excision efficiencies in primary regenerants and transmission frequencies of recombined loci to vegetative progeny as was confirmed by molecular analysis. These data might have practical implication with regard to selection of putative recombinants in vegetative progeny of potato and other clonally propagated plants as well.
Instituto de Patología Vegetal
Fil: Kopertekh, Lilya. Institute for Biosafety in Plant Biotechnology. Julius Kuehn Institute, Federal Research Centre for Cultivated Plants; Alemania
Fil: Krebs, Erika. Institute for Biosafety in Plant Biotechnology. Julius Kuehn Institute, Federal Research Centre for Cultivated Plants; Alemania
Fil: Guzman, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fuente
Plant biotechnology reports 12 (2) : 127–137. (April 2018)
Materia
Cowpea Mosaic Comovirus
Regulatory Sequences
Potatoes
Recombination
Comovirus del Mosaico de Caupi
Secuencia Reguladora
Papa
Solanum Tuberosum
Recombinación
Vegetative Progeny
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4731
Acceder
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oai_identifier_str oai:localhost:20.500.12123/4731
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virusKopertekh, LilyaKrebs, ErikaGuzman, FabianaCowpea Mosaic ComovirusRegulatory SequencesPotatoesRecombinationComovirus del Mosaico de CaupiSecuencia ReguladoraPapaSolanum TuberosumRecombinaciónVegetative ProgenyTo study the impact of regulatory sequences from Cowpea mosaic virus (CPMV) on Cre-mediated recombination rates, the cre gene was flanked by the 5′ non-translated and 3′ non-translated regions of CPMV. This cre configuration was tested by simultaneous excision of nptII selectable marker gene and heat-inducible cre recombinase gene in potato. Fusion of the cre recombinase sequence with modified regulatory sequences of CPMV increased both the excision efficiencies in primary regenerants and transmission frequencies of recombined loci to vegetative progeny as was confirmed by molecular analysis. These data might have practical implication with regard to selection of putative recombinants in vegetative progeny of potato and other clonally propagated plants as well.Instituto de Patología VegetalFil: Kopertekh, Lilya. Institute for Biosafety in Plant Biotechnology. Julius Kuehn Institute, Federal Research Centre for Cultivated Plants; AlemaniaFil: Krebs, Erika. Institute for Biosafety in Plant Biotechnology. Julius Kuehn Institute, Federal Research Centre for Cultivated Plants; AlemaniaFil: Guzman, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaSpringer2019-03-25T18:02:03Z2019-03-25T18:02:03Z2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/4731https://link.springer.com/article/10.1007%2Fs11816-018-0477-81863-54661863-5474 (Online)https://doi.org/10.1007/s11816-018-0477-8Plant biotechnology reports 12 (2) : 127–137. (April 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:54Zoai:localhost:20.500.12123/4731instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:54.254INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus
title Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus
spellingShingle Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus
Kopertekh, Lilya
Cowpea Mosaic Comovirus
Regulatory Sequences
Potatoes
Recombination
Comovirus del Mosaico de Caupi
Secuencia Reguladora
Papa
Solanum Tuberosum
Recombinación
Vegetative Progeny
title_short Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus
title_full Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus
title_fullStr Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus
title_full_unstemmed Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus
title_sort Improvement of conditional Cre-lox system through application of the regulatory sequences from Cowpea mosaic virus
dc.creator.none.fl_str_mv Kopertekh, Lilya
Krebs, Erika
Guzman, Fabiana
author Kopertekh, Lilya
author_facet Kopertekh, Lilya
Krebs, Erika
Guzman, Fabiana
author_role author
author2 Krebs, Erika
Guzman, Fabiana
author2_role author
author
dc.subject.none.fl_str_mv Cowpea Mosaic Comovirus
Regulatory Sequences
Potatoes
Recombination
Comovirus del Mosaico de Caupi
Secuencia Reguladora
Papa
Solanum Tuberosum
Recombinación
Vegetative Progeny
topic Cowpea Mosaic Comovirus
Regulatory Sequences
Potatoes
Recombination
Comovirus del Mosaico de Caupi
Secuencia Reguladora
Papa
Solanum Tuberosum
Recombinación
Vegetative Progeny
dc.description.none.fl_txt_mv To study the impact of regulatory sequences from Cowpea mosaic virus (CPMV) on Cre-mediated recombination rates, the cre gene was flanked by the 5′ non-translated and 3′ non-translated regions of CPMV. This cre configuration was tested by simultaneous excision of nptII selectable marker gene and heat-inducible cre recombinase gene in potato. Fusion of the cre recombinase sequence with modified regulatory sequences of CPMV increased both the excision efficiencies in primary regenerants and transmission frequencies of recombined loci to vegetative progeny as was confirmed by molecular analysis. These data might have practical implication with regard to selection of putative recombinants in vegetative progeny of potato and other clonally propagated plants as well.
Instituto de Patología Vegetal
Fil: Kopertekh, Lilya. Institute for Biosafety in Plant Biotechnology. Julius Kuehn Institute, Federal Research Centre for Cultivated Plants; Alemania
Fil: Krebs, Erika. Institute for Biosafety in Plant Biotechnology. Julius Kuehn Institute, Federal Research Centre for Cultivated Plants; Alemania
Fil: Guzman, Fabiana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
description To study the impact of regulatory sequences from Cowpea mosaic virus (CPMV) on Cre-mediated recombination rates, the cre gene was flanked by the 5′ non-translated and 3′ non-translated regions of CPMV. This cre configuration was tested by simultaneous excision of nptII selectable marker gene and heat-inducible cre recombinase gene in potato. Fusion of the cre recombinase sequence with modified regulatory sequences of CPMV increased both the excision efficiencies in primary regenerants and transmission frequencies of recombined loci to vegetative progeny as was confirmed by molecular analysis. These data might have practical implication with regard to selection of putative recombinants in vegetative progeny of potato and other clonally propagated plants as well.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
2019-03-25T18:02:03Z
2019-03-25T18:02:03Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/4731
https://link.springer.com/article/10.1007%2Fs11816-018-0477-8
1863-5466
1863-5474 (Online)
https://doi.org/10.1007/s11816-018-0477-8
url http://hdl.handle.net/20.500.12123/4731
https://link.springer.com/article/10.1007%2Fs11816-018-0477-8
https://doi.org/10.1007/s11816-018-0477-8
identifier_str_mv 1863-5466
1863-5474 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Plant biotechnology reports 12 (2) : 127–137. (April 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.623145

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