Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum

Autores
Sanchez, Gerardo; Gerhardt, Nadia; Siciliano, Florencia; Vojnov, Adrián; Malcuit, Isabelle; Marano, María Rosa
Año de publicación
2010
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
To evaluate the role of salicylic acid (SA) in Nb-mediated hypersensitive resistance to Potato virus X (PVX) avirulent strain ROTH1 in Solanum tuberosum, we have constructed SA-deficient transgenic potato plant lines by overexpressing the bacterial enzyme salicylate hydroxylase (NahG), which degrades SA. Evaluation of these transgenic lines revealed hydrogen peroxide accumulation and spontaneous lesion formation in an age- and light-dependent manner. In concordance, NahG potato plants were more sensitive to treatment with methyl viologen, a reactive oxygen species--generating compound. In addition, when challenged with PVX ROTH1, NahG transgenic lines showed a decreased disease-resistance response to infection and were unable to induce systemic acquired resistance. However, the avirulent viral effector, the PVX 25-kDa protein, does induce expression of the pathogenesis-related gene PR-1a in NahG potato plants. Taken together, our data indicate that SA is involved in local and systemic defense responses mediated by the Nb gene in Solanum tuberosum. This is the first report to show that basal levels of SA correlate with hypersensitive resistance to PVX.
EEA San Pedro
Fil: Sánchez, Gerardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
Fil: Gerhardt, Nadia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
Fil: Siciliano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Vojnov, Adrián. Fundación Pablo Cassará; Argentina
Fil: Malcuit, Isabelle. Algentech Limited; Reino Unido. Institute of Food Research; Reino Unido
Fil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fuente
Molecular Plant-Microbe Interactions 23 (4) : 394-405. (2010)
Materia
Papa
Solanum tuberosum
Ácido Salicílico
Potatoes
Potato Virus A
Salicylic Acid
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/5317
Acceder
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oai_identifier_str oai:localhost:20.500.12123/5317
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosumSanchez, GerardoGerhardt, NadiaSiciliano, FlorenciaVojnov, AdriánMalcuit, IsabelleMarano, María RosaPapaSolanum tuberosumÁcido SalicílicoPotatoesPotato Virus ASalicylic AcidTo evaluate the role of salicylic acid (SA) in Nb-mediated hypersensitive resistance to Potato virus X (PVX) avirulent strain ROTH1 in Solanum tuberosum, we have constructed SA-deficient transgenic potato plant lines by overexpressing the bacterial enzyme salicylate hydroxylase (NahG), which degrades SA. Evaluation of these transgenic lines revealed hydrogen peroxide accumulation and spontaneous lesion formation in an age- and light-dependent manner. In concordance, NahG potato plants were more sensitive to treatment with methyl viologen, a reactive oxygen species--generating compound. In addition, when challenged with PVX ROTH1, NahG transgenic lines showed a decreased disease-resistance response to infection and were unable to induce systemic acquired resistance. However, the avirulent viral effector, the PVX 25-kDa protein, does induce expression of the pathogenesis-related gene PR-1a in NahG potato plants. Taken together, our data indicate that SA is involved in local and systemic defense responses mediated by the Nb gene in Solanum tuberosum. This is the first report to show that basal levels of SA correlate with hypersensitive resistance to PVX.EEA San PedroFil: Sánchez, Gerardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.Fil: Gerhardt, Nadia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.Fil: Siciliano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; ArgentinaFil: Vojnov, Adrián. Fundación Pablo Cassará; ArgentinaFil: Malcuit, Isabelle. Algentech Limited; Reino Unido. Institute of Food Research; Reino UnidoFil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina2019-06-14T11:31:43Z2019-06-14T11:31:43Z2010info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.apsnet.org/publications/mpmi/2010/April/Pages/23_4_394.aspxhttp://hdl.handle.net/20.500.12123/53170894-02821943-7706http://dx.doi.org/10.1094/MPMI-23-4-0394Molecular Plant-Microbe Interactions 23 (4) : 394-405. (2010)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-04T09:48:00Zoai:localhost:20.500.12123/5317instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:48:02.205INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum
title Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum
spellingShingle Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum
Sanchez, Gerardo
Papa
Solanum tuberosum
Ácido Salicílico
Potatoes
Potato Virus A
Salicylic Acid
title_short Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum
title_full Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum
title_fullStr Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum
title_full_unstemmed Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum
title_sort Salicylic acid is involved in the Nb-mediated defense responses to Potato virus X in Solanum tuberosum
dc.creator.none.fl_str_mv Sanchez, Gerardo
Gerhardt, Nadia
Siciliano, Florencia
Vojnov, Adrián
Malcuit, Isabelle
Marano, María Rosa
author Sanchez, Gerardo
author_facet Sanchez, Gerardo
Gerhardt, Nadia
Siciliano, Florencia
Vojnov, Adrián
Malcuit, Isabelle
Marano, María Rosa
author_role author
author2 Gerhardt, Nadia
Siciliano, Florencia
Vojnov, Adrián
Malcuit, Isabelle
Marano, María Rosa
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Papa
Solanum tuberosum
Ácido Salicílico
Potatoes
Potato Virus A
Salicylic Acid
topic Papa
Solanum tuberosum
Ácido Salicílico
Potatoes
Potato Virus A
Salicylic Acid
dc.description.none.fl_txt_mv To evaluate the role of salicylic acid (SA) in Nb-mediated hypersensitive resistance to Potato virus X (PVX) avirulent strain ROTH1 in Solanum tuberosum, we have constructed SA-deficient transgenic potato plant lines by overexpressing the bacterial enzyme salicylate hydroxylase (NahG), which degrades SA. Evaluation of these transgenic lines revealed hydrogen peroxide accumulation and spontaneous lesion formation in an age- and light-dependent manner. In concordance, NahG potato plants were more sensitive to treatment with methyl viologen, a reactive oxygen species--generating compound. In addition, when challenged with PVX ROTH1, NahG transgenic lines showed a decreased disease-resistance response to infection and were unable to induce systemic acquired resistance. However, the avirulent viral effector, the PVX 25-kDa protein, does induce expression of the pathogenesis-related gene PR-1a in NahG potato plants. Taken together, our data indicate that SA is involved in local and systemic defense responses mediated by the Nb gene in Solanum tuberosum. This is the first report to show that basal levels of SA correlate with hypersensitive resistance to PVX.
EEA San Pedro
Fil: Sánchez, Gerardo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria San Pedro; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
Fil: Gerhardt, Nadia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina.
Fil: Siciliano, Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
Fil: Vojnov, Adrián. Fundación Pablo Cassará; Argentina
Fil: Malcuit, Isabelle. Algentech Limited; Reino Unido. Institute of Food Research; Reino Unido
Fil: Marano, María Rosa. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Biología Molecular y Celular de Rosario; Argentina. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas; Argentina
description To evaluate the role of salicylic acid (SA) in Nb-mediated hypersensitive resistance to Potato virus X (PVX) avirulent strain ROTH1 in Solanum tuberosum, we have constructed SA-deficient transgenic potato plant lines by overexpressing the bacterial enzyme salicylate hydroxylase (NahG), which degrades SA. Evaluation of these transgenic lines revealed hydrogen peroxide accumulation and spontaneous lesion formation in an age- and light-dependent manner. In concordance, NahG potato plants were more sensitive to treatment with methyl viologen, a reactive oxygen species--generating compound. In addition, when challenged with PVX ROTH1, NahG transgenic lines showed a decreased disease-resistance response to infection and were unable to induce systemic acquired resistance. However, the avirulent viral effector, the PVX 25-kDa protein, does induce expression of the pathogenesis-related gene PR-1a in NahG potato plants. Taken together, our data indicate that SA is involved in local and systemic defense responses mediated by the Nb gene in Solanum tuberosum. This is the first report to show that basal levels of SA correlate with hypersensitive resistance to PVX.
publishDate 2010
dc.date.none.fl_str_mv 2010
2019-06-14T11:31:43Z
2019-06-14T11:31:43Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.apsnet.org/publications/mpmi/2010/April/Pages/23_4_394.aspx
http://hdl.handle.net/20.500.12123/5317
0894-0282
1943-7706
http://dx.doi.org/10.1094/MPMI-23-4-0394
url https://www.apsnet.org/publications/mpmi/2010/April/Pages/23_4_394.aspx
http://hdl.handle.net/20.500.12123/5317
http://dx.doi.org/10.1094/MPMI-23-4-0394
identifier_str_mv 0894-0282
1943-7706
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Molecular Plant-Microbe Interactions 23 (4) : 394-405. (2010)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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