Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions

Autores
Tau, Rocio Lucia; Ferreccio, Carola Maria; Bachir, Natalia Veronica; Torales, Fatima Anabel; Romera, Sonia Alejandra; Maidana, Silvina Soledad
Año de publicación
2023
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
High-throughput sequencing of genomes has expanded our knowledge of the Alphaherpesvirinae, a widely extended subfamily of DNA viruses that recombine to increase their genetic diversity. It has been acknowledged that Equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4), two alphaherpesviruses with an economic impact on the horse industry, can recombine. This work aimed to analyze interspecific recombination between all equid alphaherpesvirus species, using genomes of EHV-1, EHV-3, EHV-4, EHV-6, EHV-8, and EHV-9 available in GenBank. 14 events of recombination by RDP4 and Simplot between EHV-1 x EHV-4, EHV-1 x EHV-9, EHV-8 x EHV-1, and EHV-8 x EHV-9 were identified. 10 out of 14 events involved ORF64, a double-copy gene located at the repeat regions that codifies for the infected cell protein 4 (ICP4). Among the ICP4, recombination can be found between EHV-1 X EHV-9, EHV-8 X EHV-9, and EHV-1 X EHV-4, the former affects zebra-borne genotypes, a type of EHV-1 that infect wild equids, and the latter match with previous breakpoints reported in fields isolates. Consequently, these findings strongly suggest that ICP4 is a hotspot for recombination. This work describes novel recombination events and is the first genome-wide recombination analysis using all available equid alphaherpesvirus species genomes.
Instituto de Virología
Fil: Tau, Rocio Lucia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Tau, Rocio Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferrecio, Carola Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación en Virología; Argentina
Fil: Ferrecio, Carola Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina
Fil: Ferrecio, Carola Maria. Universidad del Salvador. Cátedra de Inmunología; Argentina
Fil: Bachir, Natalia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Bachir, Natalia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Torales, Fatima Anabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Torales, Fatima Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Romera, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romera, Sonia Alejandra. Universidad del Salvador. Cátedra de Inmunología; Argentina
Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Maidana, Silvina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maidana, Silvina Soledad. Universidad del Salvador. Cátedra de Inmunología; Argentina
Fuente
Journal of equine veterinary science (Available online 11 September 2023)
Materia
Herpesviridae
Recombinación Homóloga
Equidae
Homologous Recombination
Alphaherpesvirus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/15284
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oai_identifier_str oai:localhost:20.500.12123/15284
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regionsTau, Rocio LuciaFerreccio, Carola MariaBachir, Natalia VeronicaTorales, Fatima AnabelRomera, Sonia AlejandraMaidana, Silvina SoledadHerpesviridaeRecombinación HomólogaEquidaeHomologous RecombinationAlphaherpesvirusHigh-throughput sequencing of genomes has expanded our knowledge of the Alphaherpesvirinae, a widely extended subfamily of DNA viruses that recombine to increase their genetic diversity. It has been acknowledged that Equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4), two alphaherpesviruses with an economic impact on the horse industry, can recombine. This work aimed to analyze interspecific recombination between all equid alphaherpesvirus species, using genomes of EHV-1, EHV-3, EHV-4, EHV-6, EHV-8, and EHV-9 available in GenBank. 14 events of recombination by RDP4 and Simplot between EHV-1 x EHV-4, EHV-1 x EHV-9, EHV-8 x EHV-1, and EHV-8 x EHV-9 were identified. 10 out of 14 events involved ORF64, a double-copy gene located at the repeat regions that codifies for the infected cell protein 4 (ICP4). Among the ICP4, recombination can be found between EHV-1 X EHV-9, EHV-8 X EHV-9, and EHV-1 X EHV-4, the former affects zebra-borne genotypes, a type of EHV-1 that infect wild equids, and the latter match with previous breakpoints reported in fields isolates. Consequently, these findings strongly suggest that ICP4 is a hotspot for recombination. This work describes novel recombination events and is the first genome-wide recombination analysis using all available equid alphaherpesvirus species genomes.Instituto de VirologíaFil: Tau, Rocio Lucia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Tau, Rocio Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Ferrecio, Carola Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación en Virología; ArgentinaFil: Ferrecio, Carola Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas; ArgentinaFil: Ferrecio, Carola Maria. Universidad del Salvador. Cátedra de Inmunología; ArgentinaFil: Bachir, Natalia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Bachir, Natalia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Torales, Fatima Anabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Torales, Fatima Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Romera, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Romera, Sonia Alejandra. Universidad del Salvador. Cátedra de Inmunología; ArgentinaFil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; ArgentinaFil: Maidana, Silvina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Maidana, Silvina Soledad. Universidad del Salvador. Cátedra de Inmunología; ArgentinaElsevier2023-09-21T16:40:21Z2023-09-21T16:40:21Z2023info:eu-repo/semantics/acceptedVersioninfo:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/152840737-0806https://doi.org/10.1016/j.jevs.2023.104916Journal of equine veterinary science (Available online 11 September 2023)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-11-06T09:41:44Zoai:localhost:20.500.12123/15284instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-11-06 09:41:45.122INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions
title Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions
spellingShingle Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions
Tau, Rocio Lucia
Herpesviridae
Recombinación Homóloga
Equidae
Homologous Recombination
Alphaherpesvirus
title_short Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions
title_full Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions
title_fullStr Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions
title_full_unstemmed Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions
title_sort Comprehensive analysis of equid herpesvirus recombination : an insight into the repeat regions
dc.creator.none.fl_str_mv Tau, Rocio Lucia
Ferreccio, Carola Maria
Bachir, Natalia Veronica
Torales, Fatima Anabel
Romera, Sonia Alejandra
Maidana, Silvina Soledad
author Tau, Rocio Lucia
author_facet Tau, Rocio Lucia
Ferreccio, Carola Maria
Bachir, Natalia Veronica
Torales, Fatima Anabel
Romera, Sonia Alejandra
Maidana, Silvina Soledad
author_role author
author2 Ferreccio, Carola Maria
Bachir, Natalia Veronica
Torales, Fatima Anabel
Romera, Sonia Alejandra
Maidana, Silvina Soledad
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Herpesviridae
Recombinación Homóloga
Equidae
Homologous Recombination
Alphaherpesvirus
topic Herpesviridae
Recombinación Homóloga
Equidae
Homologous Recombination
Alphaherpesvirus
dc.description.none.fl_txt_mv High-throughput sequencing of genomes has expanded our knowledge of the Alphaherpesvirinae, a widely extended subfamily of DNA viruses that recombine to increase their genetic diversity. It has been acknowledged that Equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4), two alphaherpesviruses with an economic impact on the horse industry, can recombine. This work aimed to analyze interspecific recombination between all equid alphaherpesvirus species, using genomes of EHV-1, EHV-3, EHV-4, EHV-6, EHV-8, and EHV-9 available in GenBank. 14 events of recombination by RDP4 and Simplot between EHV-1 x EHV-4, EHV-1 x EHV-9, EHV-8 x EHV-1, and EHV-8 x EHV-9 were identified. 10 out of 14 events involved ORF64, a double-copy gene located at the repeat regions that codifies for the infected cell protein 4 (ICP4). Among the ICP4, recombination can be found between EHV-1 X EHV-9, EHV-8 X EHV-9, and EHV-1 X EHV-4, the former affects zebra-borne genotypes, a type of EHV-1 that infect wild equids, and the latter match with previous breakpoints reported in fields isolates. Consequently, these findings strongly suggest that ICP4 is a hotspot for recombination. This work describes novel recombination events and is the first genome-wide recombination analysis using all available equid alphaherpesvirus species genomes.
Instituto de Virología
Fil: Tau, Rocio Lucia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Tau, Rocio Lucia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Ferrecio, Carola Maria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Investigación en Virología; Argentina
Fil: Ferrecio, Carola Maria. Consejo Nacional de Investigaciones Cientificas y Tecnicas; Argentina
Fil: Ferrecio, Carola Maria. Universidad del Salvador. Cátedra de Inmunología; Argentina
Fil: Bachir, Natalia Veronica. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Bachir, Natalia Veronica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Torales, Fatima Anabel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Torales, Fatima Anabel. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romera, Sonia Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Romera, Sonia Alejandra. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Romera, Sonia Alejandra. Universidad del Salvador. Cátedra de Inmunología; Argentina
Fil: Maidana, Silvina Soledad. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología e Innovaciones Tecnológicas; Argentina
Fil: Maidana, Silvina Soledad. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Maidana, Silvina Soledad. Universidad del Salvador. Cátedra de Inmunología; Argentina
description High-throughput sequencing of genomes has expanded our knowledge of the Alphaherpesvirinae, a widely extended subfamily of DNA viruses that recombine to increase their genetic diversity. It has been acknowledged that Equid herpesvirus 1 (EHV-1) and equid herpesvirus 4 (EHV-4), two alphaherpesviruses with an economic impact on the horse industry, can recombine. This work aimed to analyze interspecific recombination between all equid alphaherpesvirus species, using genomes of EHV-1, EHV-3, EHV-4, EHV-6, EHV-8, and EHV-9 available in GenBank. 14 events of recombination by RDP4 and Simplot between EHV-1 x EHV-4, EHV-1 x EHV-9, EHV-8 x EHV-1, and EHV-8 x EHV-9 were identified. 10 out of 14 events involved ORF64, a double-copy gene located at the repeat regions that codifies for the infected cell protein 4 (ICP4). Among the ICP4, recombination can be found between EHV-1 X EHV-9, EHV-8 X EHV-9, and EHV-1 X EHV-4, the former affects zebra-borne genotypes, a type of EHV-1 that infect wild equids, and the latter match with previous breakpoints reported in fields isolates. Consequently, these findings strongly suggest that ICP4 is a hotspot for recombination. This work describes novel recombination events and is the first genome-wide recombination analysis using all available equid alphaherpesvirus species genomes.
publishDate 2023
dc.date.none.fl_str_mv 2023-09-21T16:40:21Z
2023-09-21T16:40:21Z
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/acceptedVersion
info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
status_str acceptedVersion
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/15284
0737-0806
https://doi.org/10.1016/j.jevs.2023.104916
url http://hdl.handle.net/20.500.12123/15284
https://doi.org/10.1016/j.jevs.2023.104916
identifier_str_mv 0737-0806
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of equine veterinary science (Available online 11 September 2023)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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