Viruses affecting chia crops and occurrence of cowpea mild mottle virus in chia-producing regions

Autores
Luciani, Cecilia Elizabeth; Brugo Carivali, María Florencia; Solis, Valentina Eva; Flores, Ceferino Rene; Conci, Vilma Cecilia; Perotto, Maria Cecilia; Celli, Marcos Giovani
Año de publicación
2024
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Chia seeds production has been growing rapidly since mid-2011 when worldwide nutrition experts highlighted the nutritional values of this superfood. However, little is known about the viral diseases affecting this crop can cause yield losses. This study aimed to investigate the epidemiology of cowpea mild mottle virus (CPMMV) in chia crops in northwestern Argentina and to identify and characterize other viruses that infect this crop. Between 2013 and 2021, symptomatic chia leaves and seeds were collected from 480 plants and analyzed for the presence of CPMMV using PTA-ELISA. Transmission through seeds of naturally infected plants was also tested. In addition, total RNA from one CPMMV-positive plant and four CPMMV-negative plants were sequenced by massive sequencing. The results showed that 17% of the chia plants were infected with CPMMV, and the virus incidence varied from 60–80% in the north to 0–5% in the south of the chia-producing fields in the northwestern region, possibly due to variations in the vector population. CPMMV was not detected in any of the 1530 seedlings that developed from the seeds obtained from infected plants. The complete genome of CPMMV was 8180 nucleotides long and shared more than 96.54% nucleotide identity with nine isolates from Brazil and the USA. In addition, the A component of five other begomoviruses were sequenced, including sida mosaic Bolivia virus 2, which is known to infect chia plants, and four other viruses detected in chia for the first time: soybean blistering mosaic virus, tomato mottle leaf distortion virus, tomato dwarf leaf virus, and Euphorbia severe leaf golden mosaic virus. This study also reports the first detection of tomato mottle leaf curl virus in Argentina and the second detection of Euphorbia severe leaf golden mosaic virus in the world. Moreover, this is the second time that an amalgavirus reported to infect chia plants in India, Salvia hispanica RNA virus 1, was sequenced. Overall, this study provides new insights into the epidemiology and molecular detection of CPMMV and other viruses that infect chia crops, which is important for developing effective strategies for the control of these diseases and for maintaining the sustainable production of this important superfood.
Instituto de Patología Vegetal
Fil: Luciani, Cecilia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Luciani, Cecilia Elizabeth.Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Brugo Carivali, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Brugo Carivali, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Solis, Valentina Eva. Universidad Nacional del Nordeste; Argentina
Fil: Solis, Valentina Eva. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Flores, Ceferino Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; Argentina
Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conci, Vilma Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Perotto, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Celli, Marcos Giovani. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fuente
Tropical Plant Pathology 49 : 257-267. (2024)
Materia
Salvia hispanica
Plant Viruses
Cowpea Mosaic Comovirus
Carlavirus
Tobamovirus
Begomovirus
Virus de las Plantas
Comovirus del Mosaico de Caupi
Argentina
Chía
Amalgavirus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/23197
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oai_identifier_str oai:localhost:20.500.12123/23197
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network_name_str INTA Digital (INTA)
spelling Viruses affecting chia crops and occurrence of cowpea mild mottle virus in chia-producing regionsLuciani, Cecilia ElizabethBrugo Carivali, María FlorenciaSolis, Valentina EvaFlores, Ceferino ReneConci, Vilma CeciliaPerotto, Maria CeciliaCelli, Marcos GiovaniSalvia hispanicaPlant VirusesCowpea Mosaic ComovirusCarlavirusTobamovirusBegomovirusVirus de las PlantasComovirus del Mosaico de CaupiArgentinaChíaAmalgavirusChia seeds production has been growing rapidly since mid-2011 when worldwide nutrition experts highlighted the nutritional values of this superfood. However, little is known about the viral diseases affecting this crop can cause yield losses. This study aimed to investigate the epidemiology of cowpea mild mottle virus (CPMMV) in chia crops in northwestern Argentina and to identify and characterize other viruses that infect this crop. Between 2013 and 2021, symptomatic chia leaves and seeds were collected from 480 plants and analyzed for the presence of CPMMV using PTA-ELISA. Transmission through seeds of naturally infected plants was also tested. In addition, total RNA from one CPMMV-positive plant and four CPMMV-negative plants were sequenced by massive sequencing. The results showed that 17% of the chia plants were infected with CPMMV, and the virus incidence varied from 60–80% in the north to 0–5% in the south of the chia-producing fields in the northwestern region, possibly due to variations in the vector population. CPMMV was not detected in any of the 1530 seedlings that developed from the seeds obtained from infected plants. The complete genome of CPMMV was 8180 nucleotides long and shared more than 96.54% nucleotide identity with nine isolates from Brazil and the USA. In addition, the A component of five other begomoviruses were sequenced, including sida mosaic Bolivia virus 2, which is known to infect chia plants, and four other viruses detected in chia for the first time: soybean blistering mosaic virus, tomato mottle leaf distortion virus, tomato dwarf leaf virus, and Euphorbia severe leaf golden mosaic virus. This study also reports the first detection of tomato mottle leaf curl virus in Argentina and the second detection of Euphorbia severe leaf golden mosaic virus in the world. Moreover, this is the second time that an amalgavirus reported to infect chia plants in India, Salvia hispanica RNA virus 1, was sequenced. Overall, this study provides new insights into the epidemiology and molecular detection of CPMMV and other viruses that infect chia crops, which is important for developing effective strategies for the control of these diseases and for maintaining the sustainable production of this important superfood.Instituto de Patología VegetalFil: Luciani, Cecilia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Luciani, Cecilia Elizabeth.Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Brugo Carivali, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Brugo Carivali, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Solis, Valentina Eva. Universidad Nacional del Nordeste; ArgentinaFil: Solis, Valentina Eva. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Flores, Ceferino Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; ArgentinaFil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Conci, Vilma Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Perotto, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); ArgentinaFil: Celli, Marcos Giovani. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaSpringer2025-07-29T11:14:52Z2025-07-29T11:14:52Z2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/23197https://link.springer.com/article/10.1007/s40858-023-00626-01982-5676https://doi.org/10.1007/s40858-023-00626-0Tropical Plant Pathology 49 : 257-267. (2024)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:47:26Zoai:localhost:20.500.12123/23197instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:47:26.514INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Viruses affecting chia crops and occurrence of cowpea mild mottle virus in chia-producing regions
title Viruses affecting chia crops and occurrence of cowpea mild mottle virus in chia-producing regions
spellingShingle Viruses affecting chia crops and occurrence of cowpea mild mottle virus in chia-producing regions
Luciani, Cecilia Elizabeth
Salvia hispanica
Plant Viruses
Cowpea Mosaic Comovirus
Carlavirus
Tobamovirus
Begomovirus
Virus de las Plantas
Comovirus del Mosaico de Caupi
Argentina
Chía
Amalgavirus
title_short Viruses affecting chia crops and occurrence of cowpea mild mottle virus in chia-producing regions
title_full Viruses affecting chia crops and occurrence of cowpea mild mottle virus in chia-producing regions
title_fullStr Viruses affecting chia crops and occurrence of cowpea mild mottle virus in chia-producing regions
title_full_unstemmed Viruses affecting chia crops and occurrence of cowpea mild mottle virus in chia-producing regions
title_sort Viruses affecting chia crops and occurrence of cowpea mild mottle virus in chia-producing regions
dc.creator.none.fl_str_mv Luciani, Cecilia Elizabeth
Brugo Carivali, María Florencia
Solis, Valentina Eva
Flores, Ceferino Rene
Conci, Vilma Cecilia
Perotto, Maria Cecilia
Celli, Marcos Giovani
author Luciani, Cecilia Elizabeth
author_facet Luciani, Cecilia Elizabeth
Brugo Carivali, María Florencia
Solis, Valentina Eva
Flores, Ceferino Rene
Conci, Vilma Cecilia
Perotto, Maria Cecilia
Celli, Marcos Giovani
author_role author
author2 Brugo Carivali, María Florencia
Solis, Valentina Eva
Flores, Ceferino Rene
Conci, Vilma Cecilia
Perotto, Maria Cecilia
Celli, Marcos Giovani
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Salvia hispanica
Plant Viruses
Cowpea Mosaic Comovirus
Carlavirus
Tobamovirus
Begomovirus
Virus de las Plantas
Comovirus del Mosaico de Caupi
Argentina
Chía
Amalgavirus
topic Salvia hispanica
Plant Viruses
Cowpea Mosaic Comovirus
Carlavirus
Tobamovirus
Begomovirus
Virus de las Plantas
Comovirus del Mosaico de Caupi
Argentina
Chía
Amalgavirus
dc.description.none.fl_txt_mv Chia seeds production has been growing rapidly since mid-2011 when worldwide nutrition experts highlighted the nutritional values of this superfood. However, little is known about the viral diseases affecting this crop can cause yield losses. This study aimed to investigate the epidemiology of cowpea mild mottle virus (CPMMV) in chia crops in northwestern Argentina and to identify and characterize other viruses that infect this crop. Between 2013 and 2021, symptomatic chia leaves and seeds were collected from 480 plants and analyzed for the presence of CPMMV using PTA-ELISA. Transmission through seeds of naturally infected plants was also tested. In addition, total RNA from one CPMMV-positive plant and four CPMMV-negative plants were sequenced by massive sequencing. The results showed that 17% of the chia plants were infected with CPMMV, and the virus incidence varied from 60–80% in the north to 0–5% in the south of the chia-producing fields in the northwestern region, possibly due to variations in the vector population. CPMMV was not detected in any of the 1530 seedlings that developed from the seeds obtained from infected plants. The complete genome of CPMMV was 8180 nucleotides long and shared more than 96.54% nucleotide identity with nine isolates from Brazil and the USA. In addition, the A component of five other begomoviruses were sequenced, including sida mosaic Bolivia virus 2, which is known to infect chia plants, and four other viruses detected in chia for the first time: soybean blistering mosaic virus, tomato mottle leaf distortion virus, tomato dwarf leaf virus, and Euphorbia severe leaf golden mosaic virus. This study also reports the first detection of tomato mottle leaf curl virus in Argentina and the second detection of Euphorbia severe leaf golden mosaic virus in the world. Moreover, this is the second time that an amalgavirus reported to infect chia plants in India, Salvia hispanica RNA virus 1, was sequenced. Overall, this study provides new insights into the epidemiology and molecular detection of CPMMV and other viruses that infect chia crops, which is important for developing effective strategies for the control of these diseases and for maintaining the sustainable production of this important superfood.
Instituto de Patología Vegetal
Fil: Luciani, Cecilia Elizabeth. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Luciani, Cecilia Elizabeth.Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Brugo Carivali, Maria Florencia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Brugo Carivali, Maria Florencia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Solis, Valentina Eva. Universidad Nacional del Nordeste; Argentina
Fil: Solis, Valentina Eva. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Flores, Ceferino Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; Argentina
Fil: Conci, Vilma Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Conci, Vilma Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Perotto, Maria Cecilia. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas. Unidad de Fitopatología y Modelización Agrícola (UFyMA); Argentina
Fil: Celli, Marcos Giovani. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
description Chia seeds production has been growing rapidly since mid-2011 when worldwide nutrition experts highlighted the nutritional values of this superfood. However, little is known about the viral diseases affecting this crop can cause yield losses. This study aimed to investigate the epidemiology of cowpea mild mottle virus (CPMMV) in chia crops in northwestern Argentina and to identify and characterize other viruses that infect this crop. Between 2013 and 2021, symptomatic chia leaves and seeds were collected from 480 plants and analyzed for the presence of CPMMV using PTA-ELISA. Transmission through seeds of naturally infected plants was also tested. In addition, total RNA from one CPMMV-positive plant and four CPMMV-negative plants were sequenced by massive sequencing. The results showed that 17% of the chia plants were infected with CPMMV, and the virus incidence varied from 60–80% in the north to 0–5% in the south of the chia-producing fields in the northwestern region, possibly due to variations in the vector population. CPMMV was not detected in any of the 1530 seedlings that developed from the seeds obtained from infected plants. The complete genome of CPMMV was 8180 nucleotides long and shared more than 96.54% nucleotide identity with nine isolates from Brazil and the USA. In addition, the A component of five other begomoviruses were sequenced, including sida mosaic Bolivia virus 2, which is known to infect chia plants, and four other viruses detected in chia for the first time: soybean blistering mosaic virus, tomato mottle leaf distortion virus, tomato dwarf leaf virus, and Euphorbia severe leaf golden mosaic virus. This study also reports the first detection of tomato mottle leaf curl virus in Argentina and the second detection of Euphorbia severe leaf golden mosaic virus in the world. Moreover, this is the second time that an amalgavirus reported to infect chia plants in India, Salvia hispanica RNA virus 1, was sequenced. Overall, this study provides new insights into the epidemiology and molecular detection of CPMMV and other viruses that infect chia crops, which is important for developing effective strategies for the control of these diseases and for maintaining the sustainable production of this important superfood.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025-07-29T11:14:52Z
2025-07-29T11:14:52Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/23197
https://link.springer.com/article/10.1007/s40858-023-00626-0
1982-5676
https://doi.org/10.1007/s40858-023-00626-0
url http://hdl.handle.net/20.500.12123/23197
https://link.springer.com/article/10.1007/s40858-023-00626-0
https://doi.org/10.1007/s40858-023-00626-0
identifier_str_mv 1982-5676
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv restrictedAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Tropical Plant Pathology 49 : 257-267. (2024)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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