Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions?

Autores
Robert, German; Muñoz, Nacira Belen; Alvarado-Affantranger, Xochitl; Saavedra, Laura; Davidenco, Vanina; Rodríguez-Kessler, Margarita; Estrada-Navarrete, Georgina; Sánchez, Federico; Lascano, Hernán Ramiro
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Root hair curling is an early and essential morphological change required for the success of the symbiotic interaction between legumes and rhizobia. At this stage rhizobia grow as an infection thread within root hairs and are internalized into the plant cells by endocytosis, where the PI3K enzyme plays important roles. Previous observations show that stress conditions affect early stages of the symbiotic interaction, from 2 to 30 min post-inoculation, which we term as very early host responses, and affect symbiosis establishment. Herein, we demonstrated the relevance of the very early host responses for the symbiotic interaction. PI3K and the NADPH oxidase complex are found to have key roles in the microsymbiont recognition response, modulating the apoplastic and intracellular/endosomal ROS induction in root hairs. Interestingly, compared with soybean mutant plants that do not perceive the symbiont, we demonstrated that the very early symbiont perception under sublethal saline stress conditions induced root hair death. Together, these results highlight not only the importance of the very early host-responses on later stages of the symbiont interaction, but also suggest that they act as a mechanism for local control of nodulation capacity, prior to the abortion of the infection thread, preventing the allocation of resources/energy for nodule formation under unfavorable environmental conditions.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Alvarado-Affantranger, Xochitl. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Saavedra, Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Davidenco, Vanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Rodríguez-Kessler, Margarita. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Estrada-Navarrete, Georgina. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Lascano, Hernan Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fuente
Journal of Experimental Botany 69 (8) : 2037–2048. (April 2018)
Materia
Estrés Abiótico
Nodulación
Fosfatidilinositoles
Simbiontico
Abiotic Stress
Root Nodulation
Phosphatidylinositols
Symbionts
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/7447
Acceder
id INTADig_f5ea7dd5fedb4f39b925480ab908d3b2
oai_identifier_str oai:localhost:20.500.12123/7447
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions?Robert, GermanMuñoz, Nacira BelenAlvarado-Affantranger, XochitlSaavedra, LauraDavidenco, VaninaRodríguez-Kessler, MargaritaEstrada-Navarrete, GeorginaSánchez, FedericoLascano, Hernán RamiroEstrés AbióticoNodulaciónFosfatidilinositolesSimbionticoAbiotic StressRoot NodulationPhosphatidylinositolsSymbiontsRoot hair curling is an early and essential morphological change required for the success of the symbiotic interaction between legumes and rhizobia. At this stage rhizobia grow as an infection thread within root hairs and are internalized into the plant cells by endocytosis, where the PI3K enzyme plays important roles. Previous observations show that stress conditions affect early stages of the symbiotic interaction, from 2 to 30 min post-inoculation, which we term as very early host responses, and affect symbiosis establishment. Herein, we demonstrated the relevance of the very early host responses for the symbiotic interaction. PI3K and the NADPH oxidase complex are found to have key roles in the microsymbiont recognition response, modulating the apoplastic and intracellular/endosomal ROS induction in root hairs. Interestingly, compared with soybean mutant plants that do not perceive the symbiont, we demonstrated that the very early symbiont perception under sublethal saline stress conditions induced root hair death. Together, these results highlight not only the importance of the very early host-responses on later stages of the symbiont interaction, but also suggest that they act as a mechanism for local control of nodulation capacity, prior to the abortion of the infection thread, preventing the allocation of resources/energy for nodule formation under unfavorable environmental conditions.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Alvarado-Affantranger, Xochitl. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; MéxicoFil: Saavedra, Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Davidenco, Vanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Rodríguez-Kessler, Margarita. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; MéxicoFil: Estrada-Navarrete, Georgina. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; MéxicoFil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; MéxicoFil: Lascano, Hernan Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaSociety for Experimental Biology2020-06-22T11:44:48Z2020-06-22T11:44:48Z2018-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7447https://academic.oup.com/jxb/article/69/8/2037/48311160022-09571460-2431https://doi.org/10.1093/jxb/ery030Journal of Experimental Botany 69 (8) : 2037–2048. (April 2018)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:58Zoai:localhost:20.500.12123/7447instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:58.463INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions?
title Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions?
spellingShingle Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions?
Robert, German
Estrés Abiótico
Nodulación
Fosfatidilinositoles
Simbiontico
Abiotic Stress
Root Nodulation
Phosphatidylinositols
Symbionts
title_short Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions?
title_full Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions?
title_fullStr Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions?
title_full_unstemmed Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions?
title_sort Phosphatidylinositol 3-kinase function at very early symbiont perception: a local nodulation control under stress conditions?
dc.creator.none.fl_str_mv Robert, German
Muñoz, Nacira Belen
Alvarado-Affantranger, Xochitl
Saavedra, Laura
Davidenco, Vanina
Rodríguez-Kessler, Margarita
Estrada-Navarrete, Georgina
Sánchez, Federico
Lascano, Hernán Ramiro
author Robert, German
author_facet Robert, German
Muñoz, Nacira Belen
Alvarado-Affantranger, Xochitl
Saavedra, Laura
Davidenco, Vanina
Rodríguez-Kessler, Margarita
Estrada-Navarrete, Georgina
Sánchez, Federico
Lascano, Hernán Ramiro
author_role author
author2 Muñoz, Nacira Belen
Alvarado-Affantranger, Xochitl
Saavedra, Laura
Davidenco, Vanina
Rodríguez-Kessler, Margarita
Estrada-Navarrete, Georgina
Sánchez, Federico
Lascano, Hernán Ramiro
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Estrés Abiótico
Nodulación
Fosfatidilinositoles
Simbiontico
Abiotic Stress
Root Nodulation
Phosphatidylinositols
Symbionts
topic Estrés Abiótico
Nodulación
Fosfatidilinositoles
Simbiontico
Abiotic Stress
Root Nodulation
Phosphatidylinositols
Symbionts
dc.description.none.fl_txt_mv Root hair curling is an early and essential morphological change required for the success of the symbiotic interaction between legumes and rhizobia. At this stage rhizobia grow as an infection thread within root hairs and are internalized into the plant cells by endocytosis, where the PI3K enzyme plays important roles. Previous observations show that stress conditions affect early stages of the symbiotic interaction, from 2 to 30 min post-inoculation, which we term as very early host responses, and affect symbiosis establishment. Herein, we demonstrated the relevance of the very early host responses for the symbiotic interaction. PI3K and the NADPH oxidase complex are found to have key roles in the microsymbiont recognition response, modulating the apoplastic and intracellular/endosomal ROS induction in root hairs. Interestingly, compared with soybean mutant plants that do not perceive the symbiont, we demonstrated that the very early symbiont perception under sublethal saline stress conditions induced root hair death. Together, these results highlight not only the importance of the very early host-responses on later stages of the symbiont interaction, but also suggest that they act as a mechanism for local control of nodulation capacity, prior to the abortion of the infection thread, preventing the allocation of resources/energy for nodule formation under unfavorable environmental conditions.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Robert, German. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Alvarado-Affantranger, Xochitl. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Saavedra, Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Davidenco, Vanina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Rodríguez-Kessler, Margarita. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Estrada-Navarrete, Georgina. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología. Departamento de Biología Molecular de Plantas; México
Fil: Lascano, Hernan Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
description Root hair curling is an early and essential morphological change required for the success of the symbiotic interaction between legumes and rhizobia. At this stage rhizobia grow as an infection thread within root hairs and are internalized into the plant cells by endocytosis, where the PI3K enzyme plays important roles. Previous observations show that stress conditions affect early stages of the symbiotic interaction, from 2 to 30 min post-inoculation, which we term as very early host responses, and affect symbiosis establishment. Herein, we demonstrated the relevance of the very early host responses for the symbiotic interaction. PI3K and the NADPH oxidase complex are found to have key roles in the microsymbiont recognition response, modulating the apoplastic and intracellular/endosomal ROS induction in root hairs. Interestingly, compared with soybean mutant plants that do not perceive the symbiont, we demonstrated that the very early symbiont perception under sublethal saline stress conditions induced root hair death. Together, these results highlight not only the importance of the very early host-responses on later stages of the symbiont interaction, but also suggest that they act as a mechanism for local control of nodulation capacity, prior to the abortion of the infection thread, preventing the allocation of resources/energy for nodule formation under unfavorable environmental conditions.
publishDate 2018
dc.date.none.fl_str_mv 2018-04
2020-06-22T11:44:48Z
2020-06-22T11:44:48Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7447
https://academic.oup.com/jxb/article/69/8/2037/4831116
0022-0957
1460-2431
https://doi.org/10.1093/jxb/ery030
url http://hdl.handle.net/20.500.12123/7447
https://academic.oup.com/jxb/article/69/8/2037/4831116
https://doi.org/10.1093/jxb/ery030
identifier_str_mv 0022-0957
1460-2431
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Society for Experimental Biology
publisher.none.fl_str_mv Society for Experimental Biology
dc.source.none.fl_str_mv Journal of Experimental Botany 69 (8) : 2037–2048. (April 2018)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
_version_ 1844619145205776384
score 12.559606

Publicaciones similares