Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens

Autores
Agudelo Romero, Patricia; Margarida Fortes, Ana; Suárez, Trinidad; Lascano, Hernan Ramiro; Saavedra, Laura
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Phosphatidylinositol 3-phosphate (PtdIns3P) is a signaling phospholipid, which regulates several aspects of plant growth and development, as well as responses to biotic and abiotic stresses. The mechanistic insights underlying PtdIns3P mode of action, specifically through effector proteins have been partially explored in plants, with main focus on Arabidopsis thaliana. In this study, we searched for genes coding for PtdIns3P-binding proteins such as FYVE and PHOX domain-containing sequences from different photosynthetic organisms to gather evolutionary insights on these phosphoinositide binding domains, followed by an in silico characterization of the FYVE and PHOX gene families in the moss Physcomitrella patens. Phylogenetic analysis showed that PpFYVE proteins can be grouped in 7 subclasses, with an additional subclass whose FYVE domain was lost during evolution to higher plants. On the other hand, PpPHOX proteins are classified into 5 subclasses. Expression analyses based on RNAseq data together with the analysis of cis-acting regulatory elements and transcription factor (TF) binding sites in promoter regions suggest the importance of these proteins in regulating stress responses but mainly developmental processes in P. patens. The results provide valuable information and robust candidate genes for future functional analysis aiming to further explore the role of this signaling pathway mainly during growth and development of tip growing cells and during the transition from 2 to 3D growth. These studies would identify ancestral regulatory players undertaken during plant evolution.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Agudelo Romero, Patricia. University of Western Australia. Institute of Agriculture; Australia. University of Western Australia. ARC Centre of Excellence in Plant Energy Biology; Australia
Fil: Margarida Fortes, Ana. Universidade de Lisboa. Faculdade de Ciências. BioISI-Biosystems and Integrative Sciences Institute; Portugal
Fil: Suárez, Trinidad. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Saavedra, Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas (IIByT). Universidad Nacional de Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
Fuente
Planta 251 : Article number 62 (2020)
Materia
Bryophyta
Fosfatidilinositoles
Genética
Phosphatidylinositols
Genetics
Physcomitrella patens
Musgos
Mosses
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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spelling Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patensAgudelo Romero, PatriciaMargarida Fortes, AnaSuárez, TrinidadLascano, Hernan RamiroSaavedra, LauraBryophytaFosfatidilinositolesGenéticaPhosphatidylinositolsGeneticsPhyscomitrella patensMusgosMossesPhosphatidylinositol 3-phosphate (PtdIns3P) is a signaling phospholipid, which regulates several aspects of plant growth and development, as well as responses to biotic and abiotic stresses. The mechanistic insights underlying PtdIns3P mode of action, specifically through effector proteins have been partially explored in plants, with main focus on Arabidopsis thaliana. In this study, we searched for genes coding for PtdIns3P-binding proteins such as FYVE and PHOX domain-containing sequences from different photosynthetic organisms to gather evolutionary insights on these phosphoinositide binding domains, followed by an in silico characterization of the FYVE and PHOX gene families in the moss Physcomitrella patens. Phylogenetic analysis showed that PpFYVE proteins can be grouped in 7 subclasses, with an additional subclass whose FYVE domain was lost during evolution to higher plants. On the other hand, PpPHOX proteins are classified into 5 subclasses. Expression analyses based on RNAseq data together with the analysis of cis-acting regulatory elements and transcription factor (TF) binding sites in promoter regions suggest the importance of these proteins in regulating stress responses but mainly developmental processes in P. patens. The results provide valuable information and robust candidate genes for future functional analysis aiming to further explore the role of this signaling pathway mainly during growth and development of tip growing cells and during the transition from 2 to 3D growth. These studies would identify ancestral regulatory players undertaken during plant evolution.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Agudelo Romero, Patricia. University of Western Australia. Institute of Agriculture; Australia. University of Western Australia. ARC Centre of Excellence in Plant Energy Biology; AustraliaFil: Margarida Fortes, Ana. Universidade de Lisboa. Faculdade de Ciências. BioISI-Biosystems and Integrative Sciences Institute; PortugalFil: Suárez, Trinidad. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Saavedra, Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas (IIByT). Universidad Nacional de Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; ArgentinaSpringer2020-04-13T13:53:12Z2020-04-13T13:53:12Z2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/7067https://link.springer.com/article/10.1007%2Fs00425-020-03354-w0032-09351432-2048https://doi.org/10.1007/s00425-020-03354-wPlanta 251 : Article number 62 (2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:55Zoai:localhost:20.500.12123/7067instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:55.454INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
title Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
spellingShingle Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
Agudelo Romero, Patricia
Bryophyta
Fosfatidilinositoles
Genética
Phosphatidylinositols
Genetics
Physcomitrella patens
Musgos
Mosses
title_short Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
title_full Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
title_fullStr Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
title_full_unstemmed Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
title_sort Evolutionary insights into FYVE and PHOX effector proteins from the moss Physcomitrella patens
dc.creator.none.fl_str_mv Agudelo Romero, Patricia
Margarida Fortes, Ana
Suárez, Trinidad
Lascano, Hernan Ramiro
Saavedra, Laura
author Agudelo Romero, Patricia
author_facet Agudelo Romero, Patricia
Margarida Fortes, Ana
Suárez, Trinidad
Lascano, Hernan Ramiro
Saavedra, Laura
author_role author
author2 Margarida Fortes, Ana
Suárez, Trinidad
Lascano, Hernan Ramiro
Saavedra, Laura
author2_role author
author
author
author
dc.subject.none.fl_str_mv Bryophyta
Fosfatidilinositoles
Genética
Phosphatidylinositols
Genetics
Physcomitrella patens
Musgos
Mosses
topic Bryophyta
Fosfatidilinositoles
Genética
Phosphatidylinositols
Genetics
Physcomitrella patens
Musgos
Mosses
dc.description.none.fl_txt_mv Phosphatidylinositol 3-phosphate (PtdIns3P) is a signaling phospholipid, which regulates several aspects of plant growth and development, as well as responses to biotic and abiotic stresses. The mechanistic insights underlying PtdIns3P mode of action, specifically through effector proteins have been partially explored in plants, with main focus on Arabidopsis thaliana. In this study, we searched for genes coding for PtdIns3P-binding proteins such as FYVE and PHOX domain-containing sequences from different photosynthetic organisms to gather evolutionary insights on these phosphoinositide binding domains, followed by an in silico characterization of the FYVE and PHOX gene families in the moss Physcomitrella patens. Phylogenetic analysis showed that PpFYVE proteins can be grouped in 7 subclasses, with an additional subclass whose FYVE domain was lost during evolution to higher plants. On the other hand, PpPHOX proteins are classified into 5 subclasses. Expression analyses based on RNAseq data together with the analysis of cis-acting regulatory elements and transcription factor (TF) binding sites in promoter regions suggest the importance of these proteins in regulating stress responses but mainly developmental processes in P. patens. The results provide valuable information and robust candidate genes for future functional analysis aiming to further explore the role of this signaling pathway mainly during growth and development of tip growing cells and during the transition from 2 to 3D growth. These studies would identify ancestral regulatory players undertaken during plant evolution.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Agudelo Romero, Patricia. University of Western Australia. Institute of Agriculture; Australia. University of Western Australia. ARC Centre of Excellence in Plant Energy Biology; Australia
Fil: Margarida Fortes, Ana. Universidade de Lisboa. Faculdade de Ciências. BioISI-Biosystems and Integrative Sciences Institute; Portugal
Fil: Suárez, Trinidad. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Saavedra, Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto de Investigaciones Biológicas y Tecnológicas (IIByT). Universidad Nacional de Córdoba. Instituto de Investigaciones Biológicas y Tecnológicas; Argentina
description Phosphatidylinositol 3-phosphate (PtdIns3P) is a signaling phospholipid, which regulates several aspects of plant growth and development, as well as responses to biotic and abiotic stresses. The mechanistic insights underlying PtdIns3P mode of action, specifically through effector proteins have been partially explored in plants, with main focus on Arabidopsis thaliana. In this study, we searched for genes coding for PtdIns3P-binding proteins such as FYVE and PHOX domain-containing sequences from different photosynthetic organisms to gather evolutionary insights on these phosphoinositide binding domains, followed by an in silico characterization of the FYVE and PHOX gene families in the moss Physcomitrella patens. Phylogenetic analysis showed that PpFYVE proteins can be grouped in 7 subclasses, with an additional subclass whose FYVE domain was lost during evolution to higher plants. On the other hand, PpPHOX proteins are classified into 5 subclasses. Expression analyses based on RNAseq data together with the analysis of cis-acting regulatory elements and transcription factor (TF) binding sites in promoter regions suggest the importance of these proteins in regulating stress responses but mainly developmental processes in P. patens. The results provide valuable information and robust candidate genes for future functional analysis aiming to further explore the role of this signaling pathway mainly during growth and development of tip growing cells and during the transition from 2 to 3D growth. These studies would identify ancestral regulatory players undertaken during plant evolution.
publishDate 2020
dc.date.none.fl_str_mv 2020-04-13T13:53:12Z
2020-04-13T13:53:12Z
2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/7067
https://link.springer.com/article/10.1007%2Fs00425-020-03354-w
0032-0935
1432-2048
https://doi.org/10.1007/s00425-020-03354-w
url http://hdl.handle.net/20.500.12123/7067
https://link.springer.com/article/10.1007%2Fs00425-020-03354-w
https://doi.org/10.1007/s00425-020-03354-w
identifier_str_mv 0032-0935
1432-2048
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Planta 251 : Article number 62 (2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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