Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress

Autores
Muñoz, Nacira Belen; Soria Díaz, Maria Eugenia; Manyani, Hamid; Contreras Sánchez Matamoros, Rocío; Gil Serrano, Antonio; Megías, Manuel; Lascano, Hernan Ramiro
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The establishment of a symbiotic interaction involves a signal exchange between the host legume (flavonoids) and the nitrogen-fixing rhizobia (nodulation factors (NFs)). Likewise, abiotic stress conditions, such as salinity and drought, strongly reduce the nodulation process, possibly affecting also the signal exchange. In this work we characterized the structure and biological activity of NFs produced by Bradyrhizobium japonicum USDA 138 under control, salt, and osmotic stress conditions. This strain is the most widely used in Argentine soybean culture; under control conditions, it produces a mixture of four types of NFs (V(C16:0,MeFuc), V(C18:1,MeFuc), IV(C18:1), and V(C18:1,Ac,MeFuc)). Interestingly, under stress conditions, this strain produces new types of NFs, one common for both stress conditions (V(C16:1,MeFuc)) and another one only present under salt stress (IV(C18:1,MeFuc)). All mixtures of NFs, extracted from control, salt, and osmotic stress conditions, showed biological activity in soybean plants, such as root hair deformation, and the radical application of purified NFs induced systemic differences in dry matter accumulation. The inoculation of soybean with genistein-induced bacteria cultured under both control and stress conditions had a positive effect on the number of nodules formed and in some cases on dry matter accumulation. These responses are not related to changes in chlorophyll fluorescence or greenness index.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Soria Díaz, Maria Eugenia. Universidad de Sevilla. Centro de Investigación, Tecnología e Innovación. Servicio de Espectrometría de Masas; España. Universidad de Sevilla. Departamento de Química Orgánica; España
Fil: Manyani, Hamid. Universidad de Sevilla. Departamento de Microbiología y Parasitología; España
Fil: Contreras Sánchez Matamoros, Rocío. Universidad de Sevilla. Departamento de Química Orgánica; España
Fil: Gil Serrano, Antonio. Universidad de Sevilla. Departamento de Química Orgánica; España
Fil: Megías, Manuel. Universidad de Sevilla. Departamento de Microbiología y Parasitología; España
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fuente
Biology and Fertility of Soils 50 (2) : 207–215 (February 2014)
Materia
Soja
Bradyrhizobium japonicum
Estrés Osmótico
Nodulación
Estrés Abiótico
Contenido de Materia Seca
Soybeans
Osmotic Stress
Root Nodulation
Abiotic Stress
Dry Matter Content
Estres Salino
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3765
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3765
network_acronym_str INTADig
repository_id_str l
network_name_str INTA Digital (INTA)
spelling Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stressMuñoz, Nacira BelenSoria Díaz, Maria EugeniaManyani, HamidContreras Sánchez Matamoros, RocíoGil Serrano, AntonioMegías, ManuelLascano, Hernan RamiroSojaBradyrhizobium japonicumEstrés OsmóticoNodulaciónEstrés AbióticoContenido de Materia SecaSoybeansOsmotic StressRoot NodulationAbiotic StressDry Matter ContentEstres SalinoThe establishment of a symbiotic interaction involves a signal exchange between the host legume (flavonoids) and the nitrogen-fixing rhizobia (nodulation factors (NFs)). Likewise, abiotic stress conditions, such as salinity and drought, strongly reduce the nodulation process, possibly affecting also the signal exchange. In this work we characterized the structure and biological activity of NFs produced by Bradyrhizobium japonicum USDA 138 under control, salt, and osmotic stress conditions. This strain is the most widely used in Argentine soybean culture; under control conditions, it produces a mixture of four types of NFs (V(C16:0,MeFuc), V(C18:1,MeFuc), IV(C18:1), and V(C18:1,Ac,MeFuc)). Interestingly, under stress conditions, this strain produces new types of NFs, one common for both stress conditions (V(C16:1,MeFuc)) and another one only present under salt stress (IV(C18:1,MeFuc)). All mixtures of NFs, extracted from control, salt, and osmotic stress conditions, showed biological activity in soybean plants, such as root hair deformation, and the radical application of purified NFs induced systemic differences in dry matter accumulation. The inoculation of soybean with genistein-induced bacteria cultured under both control and stress conditions had a positive effect on the number of nodules formed and in some cases on dry matter accumulation. These responses are not related to changes in chlorophyll fluorescence or greenness index.Instituto de Fisiología y Recursos Genéticos VegetalesFil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; ArgentinaFil: Soria Díaz, Maria Eugenia. Universidad de Sevilla. Centro de Investigación, Tecnología e Innovación. Servicio de Espectrometría de Masas; España. Universidad de Sevilla. Departamento de Química Orgánica; EspañaFil: Manyani, Hamid. Universidad de Sevilla. Departamento de Microbiología y Parasitología; EspañaFil: Contreras Sánchez Matamoros, Rocío. Universidad de Sevilla. Departamento de Química Orgánica; EspañaFil: Gil Serrano, Antonio. Universidad de Sevilla. Departamento de Química Orgánica; EspañaFil: Megías, Manuel. Universidad de Sevilla. Departamento de Microbiología y Parasitología; EspañaFil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina2018-11-02T12:16:07Z2018-11-02T12:16:07Z2014-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://link.springer.com/article/10.1007/s00374-013-0843-1http://hdl.handle.net/20.500.12123/37650178-27621432-0789https://doi.org/10.1007/s00374-013-0843-1Biology and Fertility of Soils 50 (2) : 207–215 (February 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:28Zoai:localhost:20.500.12123/3765instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:29.315INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress
title Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress
spellingShingle Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress
Muñoz, Nacira Belen
Soja
Bradyrhizobium japonicum
Estrés Osmótico
Nodulación
Estrés Abiótico
Contenido de Materia Seca
Soybeans
Osmotic Stress
Root Nodulation
Abiotic Stress
Dry Matter Content
Estres Salino
title_short Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress
title_full Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress
title_fullStr Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress
title_full_unstemmed Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress
title_sort Structure and biological activities of lipochitooligosaccharide nodulation signals produced by Bradyrhizobium japonicum USDA 138 under saline and osmotic stress
dc.creator.none.fl_str_mv Muñoz, Nacira Belen
Soria Díaz, Maria Eugenia
Manyani, Hamid
Contreras Sánchez Matamoros, Rocío
Gil Serrano, Antonio
Megías, Manuel
Lascano, Hernan Ramiro
author Muñoz, Nacira Belen
author_facet Muñoz, Nacira Belen
Soria Díaz, Maria Eugenia
Manyani, Hamid
Contreras Sánchez Matamoros, Rocío
Gil Serrano, Antonio
Megías, Manuel
Lascano, Hernan Ramiro
author_role author
author2 Soria Díaz, Maria Eugenia
Manyani, Hamid
Contreras Sánchez Matamoros, Rocío
Gil Serrano, Antonio
Megías, Manuel
Lascano, Hernan Ramiro
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Soja
Bradyrhizobium japonicum
Estrés Osmótico
Nodulación
Estrés Abiótico
Contenido de Materia Seca
Soybeans
Osmotic Stress
Root Nodulation
Abiotic Stress
Dry Matter Content
Estres Salino
topic Soja
Bradyrhizobium japonicum
Estrés Osmótico
Nodulación
Estrés Abiótico
Contenido de Materia Seca
Soybeans
Osmotic Stress
Root Nodulation
Abiotic Stress
Dry Matter Content
Estres Salino
dc.description.none.fl_txt_mv The establishment of a symbiotic interaction involves a signal exchange between the host legume (flavonoids) and the nitrogen-fixing rhizobia (nodulation factors (NFs)). Likewise, abiotic stress conditions, such as salinity and drought, strongly reduce the nodulation process, possibly affecting also the signal exchange. In this work we characterized the structure and biological activity of NFs produced by Bradyrhizobium japonicum USDA 138 under control, salt, and osmotic stress conditions. This strain is the most widely used in Argentine soybean culture; under control conditions, it produces a mixture of four types of NFs (V(C16:0,MeFuc), V(C18:1,MeFuc), IV(C18:1), and V(C18:1,Ac,MeFuc)). Interestingly, under stress conditions, this strain produces new types of NFs, one common for both stress conditions (V(C16:1,MeFuc)) and another one only present under salt stress (IV(C18:1,MeFuc)). All mixtures of NFs, extracted from control, salt, and osmotic stress conditions, showed biological activity in soybean plants, such as root hair deformation, and the radical application of purified NFs induced systemic differences in dry matter accumulation. The inoculation of soybean with genistein-induced bacteria cultured under both control and stress conditions had a positive effect on the number of nodules formed and in some cases on dry matter accumulation. These responses are not related to changes in chlorophyll fluorescence or greenness index.
Instituto de Fisiología y Recursos Genéticos Vegetales
Fil: Muñoz, Nacira Belen. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
Fil: Soria Díaz, Maria Eugenia. Universidad de Sevilla. Centro de Investigación, Tecnología e Innovación. Servicio de Espectrometría de Masas; España. Universidad de Sevilla. Departamento de Química Orgánica; España
Fil: Manyani, Hamid. Universidad de Sevilla. Departamento de Microbiología y Parasitología; España
Fil: Contreras Sánchez Matamoros, Rocío. Universidad de Sevilla. Departamento de Química Orgánica; España
Fil: Gil Serrano, Antonio. Universidad de Sevilla. Departamento de Química Orgánica; España
Fil: Megías, Manuel. Universidad de Sevilla. Departamento de Microbiología y Parasitología; España
Fil: Lascano, Hernan Ramiro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas Físicas y Naturales. Cátedra de Fisiología Vegetal; Argentina
description The establishment of a symbiotic interaction involves a signal exchange between the host legume (flavonoids) and the nitrogen-fixing rhizobia (nodulation factors (NFs)). Likewise, abiotic stress conditions, such as salinity and drought, strongly reduce the nodulation process, possibly affecting also the signal exchange. In this work we characterized the structure and biological activity of NFs produced by Bradyrhizobium japonicum USDA 138 under control, salt, and osmotic stress conditions. This strain is the most widely used in Argentine soybean culture; under control conditions, it produces a mixture of four types of NFs (V(C16:0,MeFuc), V(C18:1,MeFuc), IV(C18:1), and V(C18:1,Ac,MeFuc)). Interestingly, under stress conditions, this strain produces new types of NFs, one common for both stress conditions (V(C16:1,MeFuc)) and another one only present under salt stress (IV(C18:1,MeFuc)). All mixtures of NFs, extracted from control, salt, and osmotic stress conditions, showed biological activity in soybean plants, such as root hair deformation, and the radical application of purified NFs induced systemic differences in dry matter accumulation. The inoculation of soybean with genistein-induced bacteria cultured under both control and stress conditions had a positive effect on the number of nodules formed and in some cases on dry matter accumulation. These responses are not related to changes in chlorophyll fluorescence or greenness index.
publishDate 2014
dc.date.none.fl_str_mv 2014-02
2018-11-02T12:16:07Z
2018-11-02T12:16:07Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://link.springer.com/article/10.1007/s00374-013-0843-1
http://hdl.handle.net/20.500.12123/3765
0178-2762
1432-0789
https://doi.org/10.1007/s00374-013-0843-1
url https://link.springer.com/article/10.1007/s00374-013-0843-1
http://hdl.handle.net/20.500.12123/3765
https://doi.org/10.1007/s00374-013-0843-1
identifier_str_mv 0178-2762
1432-0789
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Biology and Fertility of Soils 50 (2) : 207–215 (February 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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