Phosphatidylinositol 3-kinase function at very early symbiont perception: A local nodulation control under stress conditions?

Autores
Robert, Germán; Muñoz, Nacira Belén; Alvarado Affantranger, Xochitl; Saavedra, Laura; Davidenco, Vanina; Rodríguez y Domínguez Kessler, Margarita; Estrada Navarrete, Georgina; Sanchez, Federico; Lascano, Hernan Ramiro
Año de publicación
2018
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Root hair curling is an early and essential morphological change required for the success of the symbiotic interaction between legumes and rhizobia. At this stage rhizobia grow as an infection thread within root hairs and are internalized into the plant cells by endocytosis, where the PI3K enzyme plays important roles. Previous observations show that stress conditions affect early stages of the symbiotic interaction, from 2 to 30 min post-inoculation, which we term as very early host responses, and affect symbiosis establishment. Herein, we demonstrated the relevance of the very early host responses for the symbiotic interaction. PI3K and the NADPH oxidase complex are found to have key roles in the microsymbiont recognition response, modulating the apoplastic and intracellular/endosomal ROS induction in root hairs. Interestingly, compared with soybean mutant plants that do not perceive the symbiont, we demonstrated that the very early symbiont perception under sublethal saline stress conditions induced root hair death. Together, these results highlight not only the importance of the very early host-responses on later stages of the symbiont interaction, but also suggest that they act as a mechanism for local control of nodulation capacity, prior to the abortion of the infection thread, preventing the allocation of resources/energy for nodule formation under unfavorable environmental conditions.
Fil: Robert, Germán. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; Argentina
Fil: Muñoz, Nacira Belén. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Alvarado Affantranger, Xochitl. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Saavedra, Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; Argentina
Fil: Davidenco, Vanina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Rodríguez y Domínguez Kessler, Margarita. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Estrada Navarrete, Georgina. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Lascano, Hernan Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Materia
ABIOTIC STRESS
NODULATION
PHOSPHATIDYLINOSITOL 3-KINASE
PI3K
REACTIVE OXYGEN SPECIES
ROS
SYMBIONT PERCEPTION
SYMBIOTIC INTERACTION
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/133329
Acceder
id CONICETDig_40b62280e181d1b9ac96d2f8e0445b68
oai_identifier_str oai:ri.conicet.gov.ar:11336/133329
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Phosphatidylinositol 3-kinase function at very early symbiont perception: A local nodulation control under stress conditions?Robert, GermánMuñoz, Nacira BelénAlvarado Affantranger, XochitlSaavedra, LauraDavidenco, VaninaRodríguez y Domínguez Kessler, MargaritaEstrada Navarrete, GeorginaSanchez, FedericoLascano, Hernan RamiroABIOTIC STRESSNODULATIONPHOSPHATIDYLINOSITOL 3-KINASEPI3KREACTIVE OXYGEN SPECIESROSSYMBIONT PERCEPTIONSYMBIOTIC INTERACTIONhttps://purl.org/becyt/ford/4.4https://purl.org/becyt/ford/4Root hair curling is an early and essential morphological change required for the success of the symbiotic interaction between legumes and rhizobia. At this stage rhizobia grow as an infection thread within root hairs and are internalized into the plant cells by endocytosis, where the PI3K enzyme plays important roles. Previous observations show that stress conditions affect early stages of the symbiotic interaction, from 2 to 30 min post-inoculation, which we term as very early host responses, and affect symbiosis establishment. Herein, we demonstrated the relevance of the very early host responses for the symbiotic interaction. PI3K and the NADPH oxidase complex are found to have key roles in the microsymbiont recognition response, modulating the apoplastic and intracellular/endosomal ROS induction in root hairs. Interestingly, compared with soybean mutant plants that do not perceive the symbiont, we demonstrated that the very early symbiont perception under sublethal saline stress conditions induced root hair death. Together, these results highlight not only the importance of the very early host-responses on later stages of the symbiont interaction, but also suggest that they act as a mechanism for local control of nodulation capacity, prior to the abortion of the infection thread, preventing the allocation of resources/energy for nodule formation under unfavorable environmental conditions.Fil: Robert, Germán. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; ArgentinaFil: Muñoz, Nacira Belén. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Alvarado Affantranger, Xochitl. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Saavedra, Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; ArgentinaFil: Davidenco, Vanina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaFil: Rodríguez y Domínguez Kessler, Margarita. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Estrada Navarrete, Georgina. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología; MéxicoFil: Lascano, Hernan Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; ArgentinaOxford University Press2018-04-03info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/133329Robert, Germán; Muñoz, Nacira Belén; Alvarado Affantranger, Xochitl; Saavedra, Laura; Davidenco, Vanina; et al.; Phosphatidylinositol 3-kinase function at very early symbiont perception: A local nodulation control under stress conditions?; Oxford University Press; Journal of Experimental Botany; 69; 8; 3-4-2018; 2037-20480022-0957CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article/69/8/2037/4831116info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/ery030info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T14:44:16Zoai:ri.conicet.gov.ar:11336/133329instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 14:44:16.894CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Phosphatidylinositol 3-kinase function at very early symbiont perception: A local nodulation control under stress conditions?
title Phosphatidylinositol 3-kinase function at very early symbiont perception: A local nodulation control under stress conditions?
spellingShingle Phosphatidylinositol 3-kinase function at very early symbiont perception: A local nodulation control under stress conditions?
Robert, Germán
ABIOTIC STRESS
NODULATION
PHOSPHATIDYLINOSITOL 3-KINASE
PI3K
REACTIVE OXYGEN SPECIES
ROS
SYMBIONT PERCEPTION
SYMBIOTIC INTERACTION
title_short Phosphatidylinositol 3-kinase function at very early symbiont perception: A local nodulation control under stress conditions?
title_full Phosphatidylinositol 3-kinase function at very early symbiont perception: A local nodulation control under stress conditions?
title_fullStr Phosphatidylinositol 3-kinase function at very early symbiont perception: A local nodulation control under stress conditions?
title_full_unstemmed Phosphatidylinositol 3-kinase function at very early symbiont perception: A local nodulation control under stress conditions?
title_sort Phosphatidylinositol 3-kinase function at very early symbiont perception: A local nodulation control under stress conditions?
dc.creator.none.fl_str_mv Robert, Germán
Muñoz, Nacira Belén
Alvarado Affantranger, Xochitl
Saavedra, Laura
Davidenco, Vanina
Rodríguez y Domínguez Kessler, Margarita
Estrada Navarrete, Georgina
Sanchez, Federico
Lascano, Hernan Ramiro
author Robert, Germán
author_facet Robert, Germán
Muñoz, Nacira Belén
Alvarado Affantranger, Xochitl
Saavedra, Laura
Davidenco, Vanina
Rodríguez y Domínguez Kessler, Margarita
Estrada Navarrete, Georgina
Sanchez, Federico
Lascano, Hernan Ramiro
author_role author
author2 Muñoz, Nacira Belén
Alvarado Affantranger, Xochitl
Saavedra, Laura
Davidenco, Vanina
Rodríguez y Domínguez Kessler, Margarita
Estrada Navarrete, Georgina
Sanchez, Federico
Lascano, Hernan Ramiro
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ABIOTIC STRESS
NODULATION
PHOSPHATIDYLINOSITOL 3-KINASE
PI3K
REACTIVE OXYGEN SPECIES
ROS
SYMBIONT PERCEPTION
SYMBIOTIC INTERACTION
topic ABIOTIC STRESS
NODULATION
PHOSPHATIDYLINOSITOL 3-KINASE
PI3K
REACTIVE OXYGEN SPECIES
ROS
SYMBIONT PERCEPTION
SYMBIOTIC INTERACTION
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.4
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Root hair curling is an early and essential morphological change required for the success of the symbiotic interaction between legumes and rhizobia. At this stage rhizobia grow as an infection thread within root hairs and are internalized into the plant cells by endocytosis, where the PI3K enzyme plays important roles. Previous observations show that stress conditions affect early stages of the symbiotic interaction, from 2 to 30 min post-inoculation, which we term as very early host responses, and affect symbiosis establishment. Herein, we demonstrated the relevance of the very early host responses for the symbiotic interaction. PI3K and the NADPH oxidase complex are found to have key roles in the microsymbiont recognition response, modulating the apoplastic and intracellular/endosomal ROS induction in root hairs. Interestingly, compared with soybean mutant plants that do not perceive the symbiont, we demonstrated that the very early symbiont perception under sublethal saline stress conditions induced root hair death. Together, these results highlight not only the importance of the very early host-responses on later stages of the symbiont interaction, but also suggest that they act as a mechanism for local control of nodulation capacity, prior to the abortion of the infection thread, preventing the allocation of resources/energy for nodule formation under unfavorable environmental conditions.
Fil: Robert, Germán. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; Argentina
Fil: Muñoz, Nacira Belén. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigaciones Agropecuarias. Instituto de Fisiología y Recursos Genéticos Vegetales; Argentina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Alvarado Affantranger, Xochitl. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Saavedra, Laura. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; Argentina
Fil: Davidenco, Vanina. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
Fil: Rodríguez y Domínguez Kessler, Margarita. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Estrada Navarrete, Georgina. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Sanchez, Federico. Universidad Nacional Autónoma de México. Instituto de Biotecnología; México
Fil: Lascano, Hernan Ramiro. Universidad Nacional de Córdoba. Facultad de Ciencias Agropecuarias. Cátedra de Fisiología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba; Argentina
description Root hair curling is an early and essential morphological change required for the success of the symbiotic interaction between legumes and rhizobia. At this stage rhizobia grow as an infection thread within root hairs and are internalized into the plant cells by endocytosis, where the PI3K enzyme plays important roles. Previous observations show that stress conditions affect early stages of the symbiotic interaction, from 2 to 30 min post-inoculation, which we term as very early host responses, and affect symbiosis establishment. Herein, we demonstrated the relevance of the very early host responses for the symbiotic interaction. PI3K and the NADPH oxidase complex are found to have key roles in the microsymbiont recognition response, modulating the apoplastic and intracellular/endosomal ROS induction in root hairs. Interestingly, compared with soybean mutant plants that do not perceive the symbiont, we demonstrated that the very early symbiont perception under sublethal saline stress conditions induced root hair death. Together, these results highlight not only the importance of the very early host-responses on later stages of the symbiont interaction, but also suggest that they act as a mechanism for local control of nodulation capacity, prior to the abortion of the infection thread, preventing the allocation of resources/energy for nodule formation under unfavorable environmental conditions.
publishDate 2018
dc.date.none.fl_str_mv 2018-04-03
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/133329
Robert, Germán; Muñoz, Nacira Belén; Alvarado Affantranger, Xochitl; Saavedra, Laura; Davidenco, Vanina; et al.; Phosphatidylinositol 3-kinase function at very early symbiont perception: A local nodulation control under stress conditions?; Oxford University Press; Journal of Experimental Botany; 69; 8; 3-4-2018; 2037-2048
0022-0957
CONICET Digital
CONICET
url http://hdl.handle.net/11336/133329
identifier_str_mv Robert, Germán; Muñoz, Nacira Belén; Alvarado Affantranger, Xochitl; Saavedra, Laura; Davidenco, Vanina; et al.; Phosphatidylinositol 3-kinase function at very early symbiont perception: A local nodulation control under stress conditions?; Oxford University Press; Journal of Experimental Botany; 69; 8; 3-4-2018; 2037-2048
0022-0957
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/jxb/article/69/8/2037/4831116
info:eu-repo/semantics/altIdentifier/doi/10.1093/jxb/ery030
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.22299

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