First report of cowpea mild mottle virus in chia (Salvia hispanica)
- Autores
- Celli, Marcos Giovani; Perotto, Maria Cecilia; Merino, Maria Alicia; Nome Docampo, Claudia; Flores, Ceferino Rene; Conci, Vilma Cecilia
- Año de publicación
- 2016
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión aceptada
- Descripción
- Chia (Salvia hispanica), a herbaceous plant of Lamiaceae family, has gained popularity due to its high concentrations of health-beneficial Omega-3 fatty acids. Plants showing different virus-like symptoms were observed in the principal chia production areas in Argentina. Some plants exhibited yellowing, mottled and blistering leaves, and others shortened internodes and leaf and stem deformation. The aim of this study was to identify the viruses infecting this crop. Forty symptomatic chia plants were collected from nine production fields in northeastern Argentina. The samples were tested for Alfalfa mosaic virus (AMV), Cowpea mild mottle virus (CPMMV), Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV) and Tospovirus group (I, II and III) by DAS-ELISA, for the genera Potyvirus by PTA-ELISA and for Begomovirus by PCR. CPMMV was detected in three plants with different kind of symptoms. In these plants, feather-like inclusions formed by virions were observed with transmission electron microscopy. ORF2 to ORF6 (2462 nucleotides) from the CPMMV viral genome was amplified by RT-PCR. Nucleotide (nt) sequence of the coat-protein gene (CP – ORF5) of the chia virus isolate was obtained and compared with 31 CPMMV sequences reported in GenBank. Results showed between 75.5% and 99.0% of nt identity, which is above the International Committee on Taxonomy of Viruses criteria for Carlavirus species differentiation. The phylogenetic analysis with the CP gene nt sequences revealed that the CPMMV chia isolate grouped with other CPMMV isolates from other plant species from Brazil, Ghana, India, Puerto Rico, Taiwan and USA, but were separated from four others from India. This is the first report of the presence of CPMMV in chia in the world. Although the other viruses were not detected in this work, it is possible that the different symptoms observed could be produced by a mixture of viruses because CPMMV was found in chia plants with different symptoms.
Inst. Patología Vegetal
Fil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Merino, Maria Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nome Docampo, Claudia Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Flores, Ceferino Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; Argentina
Fil: Conci, Vilma Cecilia Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina - Fuente
- Crop protection 89 :1-5. (November 2016)
- Materia
-
Enfermedades de las Plantas
Salvia (género)
Virus de las Plantas
Plant Diseases
Plant Viruses
Chía
Salvia Hispánica - Nivel de accesibilidad
- acceso restringido
- Condiciones de uso
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/1239
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First report of cowpea mild mottle virus in chia (Salvia hispanica)Celli, Marcos GiovaniPerotto, Maria CeciliaMerino, Maria AliciaNome Docampo, ClaudiaFlores, Ceferino ReneConci, Vilma CeciliaEnfermedades de las PlantasSalvia (género)Virus de las PlantasPlant DiseasesPlant VirusesChíaSalvia HispánicaChia (Salvia hispanica), a herbaceous plant of Lamiaceae family, has gained popularity due to its high concentrations of health-beneficial Omega-3 fatty acids. Plants showing different virus-like symptoms were observed in the principal chia production areas in Argentina. Some plants exhibited yellowing, mottled and blistering leaves, and others shortened internodes and leaf and stem deformation. The aim of this study was to identify the viruses infecting this crop. Forty symptomatic chia plants were collected from nine production fields in northeastern Argentina. The samples were tested for Alfalfa mosaic virus (AMV), Cowpea mild mottle virus (CPMMV), Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV) and Tospovirus group (I, II and III) by DAS-ELISA, for the genera Potyvirus by PTA-ELISA and for Begomovirus by PCR. CPMMV was detected in three plants with different kind of symptoms. In these plants, feather-like inclusions formed by virions were observed with transmission electron microscopy. ORF2 to ORF6 (2462 nucleotides) from the CPMMV viral genome was amplified by RT-PCR. Nucleotide (nt) sequence of the coat-protein gene (CP – ORF5) of the chia virus isolate was obtained and compared with 31 CPMMV sequences reported in GenBank. Results showed between 75.5% and 99.0% of nt identity, which is above the International Committee on Taxonomy of Viruses criteria for Carlavirus species differentiation. The phylogenetic analysis with the CP gene nt sequences revealed that the CPMMV chia isolate grouped with other CPMMV isolates from other plant species from Brazil, Ghana, India, Puerto Rico, Taiwan and USA, but were separated from four others from India. This is the first report of the presence of CPMMV in chia in the world. Although the other viruses were not detected in this work, it is possible that the different symptoms observed could be produced by a mixture of viruses because CPMMV was found in chia plants with different symptoms.Inst. Patología VegetalFil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Merino, Maria Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nome Docampo, Claudia Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Flores, Ceferino Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; ArgentinaFil: Conci, Vilma Cecilia Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2017-09-18T14:34:09Z2017-09-18T14:34:09Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1239http://www.sciencedirect.com/science/article/pii/S02612194163014540261-2194https://doi.org/10.1016/j.cropro.2016.05.014Crop protection 89 :1-5. (November 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:01Zoai:localhost:20.500.12123/1239instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:03.275INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
First report of cowpea mild mottle virus in chia (Salvia hispanica) |
title |
First report of cowpea mild mottle virus in chia (Salvia hispanica) |
spellingShingle |
First report of cowpea mild mottle virus in chia (Salvia hispanica) Celli, Marcos Giovani Enfermedades de las Plantas Salvia (género) Virus de las Plantas Plant Diseases Plant Viruses Chía Salvia Hispánica |
title_short |
First report of cowpea mild mottle virus in chia (Salvia hispanica) |
title_full |
First report of cowpea mild mottle virus in chia (Salvia hispanica) |
title_fullStr |
First report of cowpea mild mottle virus in chia (Salvia hispanica) |
title_full_unstemmed |
First report of cowpea mild mottle virus in chia (Salvia hispanica) |
title_sort |
First report of cowpea mild mottle virus in chia (Salvia hispanica) |
dc.creator.none.fl_str_mv |
Celli, Marcos Giovani Perotto, Maria Cecilia Merino, Maria Alicia Nome Docampo, Claudia Flores, Ceferino Rene Conci, Vilma Cecilia |
author |
Celli, Marcos Giovani |
author_facet |
Celli, Marcos Giovani Perotto, Maria Cecilia Merino, Maria Alicia Nome Docampo, Claudia Flores, Ceferino Rene Conci, Vilma Cecilia |
author_role |
author |
author2 |
Perotto, Maria Cecilia Merino, Maria Alicia Nome Docampo, Claudia Flores, Ceferino Rene Conci, Vilma Cecilia |
author2_role |
author author author author author |
dc.subject.none.fl_str_mv |
Enfermedades de las Plantas Salvia (género) Virus de las Plantas Plant Diseases Plant Viruses Chía Salvia Hispánica |
topic |
Enfermedades de las Plantas Salvia (género) Virus de las Plantas Plant Diseases Plant Viruses Chía Salvia Hispánica |
dc.description.none.fl_txt_mv |
Chia (Salvia hispanica), a herbaceous plant of Lamiaceae family, has gained popularity due to its high concentrations of health-beneficial Omega-3 fatty acids. Plants showing different virus-like symptoms were observed in the principal chia production areas in Argentina. Some plants exhibited yellowing, mottled and blistering leaves, and others shortened internodes and leaf and stem deformation. The aim of this study was to identify the viruses infecting this crop. Forty symptomatic chia plants were collected from nine production fields in northeastern Argentina. The samples were tested for Alfalfa mosaic virus (AMV), Cowpea mild mottle virus (CPMMV), Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV) and Tospovirus group (I, II and III) by DAS-ELISA, for the genera Potyvirus by PTA-ELISA and for Begomovirus by PCR. CPMMV was detected in three plants with different kind of symptoms. In these plants, feather-like inclusions formed by virions were observed with transmission electron microscopy. ORF2 to ORF6 (2462 nucleotides) from the CPMMV viral genome was amplified by RT-PCR. Nucleotide (nt) sequence of the coat-protein gene (CP – ORF5) of the chia virus isolate was obtained and compared with 31 CPMMV sequences reported in GenBank. Results showed between 75.5% and 99.0% of nt identity, which is above the International Committee on Taxonomy of Viruses criteria for Carlavirus species differentiation. The phylogenetic analysis with the CP gene nt sequences revealed that the CPMMV chia isolate grouped with other CPMMV isolates from other plant species from Brazil, Ghana, India, Puerto Rico, Taiwan and USA, but were separated from four others from India. This is the first report of the presence of CPMMV in chia in the world. Although the other viruses were not detected in this work, it is possible that the different symptoms observed could be produced by a mixture of viruses because CPMMV was found in chia plants with different symptoms. Inst. Patología Vegetal Fil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Merino, Maria Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Nome Docampo, Claudia Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina Fil: Flores, Ceferino Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; Argentina Fil: Conci, Vilma Cecilia Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
description |
Chia (Salvia hispanica), a herbaceous plant of Lamiaceae family, has gained popularity due to its high concentrations of health-beneficial Omega-3 fatty acids. Plants showing different virus-like symptoms were observed in the principal chia production areas in Argentina. Some plants exhibited yellowing, mottled and blistering leaves, and others shortened internodes and leaf and stem deformation. The aim of this study was to identify the viruses infecting this crop. Forty symptomatic chia plants were collected from nine production fields in northeastern Argentina. The samples were tested for Alfalfa mosaic virus (AMV), Cowpea mild mottle virus (CPMMV), Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV) and Tospovirus group (I, II and III) by DAS-ELISA, for the genera Potyvirus by PTA-ELISA and for Begomovirus by PCR. CPMMV was detected in three plants with different kind of symptoms. In these plants, feather-like inclusions formed by virions were observed with transmission electron microscopy. ORF2 to ORF6 (2462 nucleotides) from the CPMMV viral genome was amplified by RT-PCR. Nucleotide (nt) sequence of the coat-protein gene (CP – ORF5) of the chia virus isolate was obtained and compared with 31 CPMMV sequences reported in GenBank. Results showed between 75.5% and 99.0% of nt identity, which is above the International Committee on Taxonomy of Viruses criteria for Carlavirus species differentiation. The phylogenetic analysis with the CP gene nt sequences revealed that the CPMMV chia isolate grouped with other CPMMV isolates from other plant species from Brazil, Ghana, India, Puerto Rico, Taiwan and USA, but were separated from four others from India. This is the first report of the presence of CPMMV in chia in the world. Although the other viruses were not detected in this work, it is possible that the different symptoms observed could be produced by a mixture of viruses because CPMMV was found in chia plants with different symptoms. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016 2017-09-18T14:34:09Z 2017-09-18T14:34:09Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
acceptedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/1239 http://www.sciencedirect.com/science/article/pii/S0261219416301454 0261-2194 https://doi.org/10.1016/j.cropro.2016.05.014 |
url |
http://hdl.handle.net/20.500.12123/1239 http://www.sciencedirect.com/science/article/pii/S0261219416301454 https://doi.org/10.1016/j.cropro.2016.05.014 |
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0261-2194 |
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eng |
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eng |
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Crop protection 89 :1-5. (November 2016) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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INTA Digital (INTA) |
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tripaldi.nicolas@inta.gob.ar |
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