First report of cowpea mild mottle virus in chia (Salvia hispanica)

Autores
Celli, Marcos Giovani; Perotto, Maria Cecilia; Merino, Maria Alicia; Nome Docampo, Claudia; Flores, Ceferino Rene; Conci, Vilma Cecilia
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Chia (Salvia hispanica), a herbaceous plant of Lamiaceae family, has gained popularity due to its high concentrations of health-beneficial Omega-3 fatty acids. Plants showing different virus-like symptoms were observed in the principal chia production areas in Argentina. Some plants exhibited yellowing, mottled and blistering leaves, and others shortened internodes and leaf and stem deformation. The aim of this study was to identify the viruses infecting this crop. Forty symptomatic chia plants were collected from nine production fields in northeastern Argentina. The samples were tested for Alfalfa mosaic virus (AMV), Cowpea mild mottle virus (CPMMV), Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV) and Tospovirus group (I, II and III) by DAS-ELISA, for the genera Potyvirus by PTA-ELISA and for Begomovirus by PCR. CPMMV was detected in three plants with different kind of symptoms. In these plants, feather-like inclusions formed by virions were observed with transmission electron microscopy. ORF2 to ORF6 (2462 nucleotides) from the CPMMV viral genome was amplified by RT-PCR. Nucleotide (nt) sequence of the coat-protein gene (CP – ORF5) of the chia virus isolate was obtained and compared with 31 CPMMV sequences reported in GenBank. Results showed between 75.5% and 99.0% of nt identity, which is above the International Committee on Taxonomy of Viruses criteria for Carlavirus species differentiation. The phylogenetic analysis with the CP gene nt sequences revealed that the CPMMV chia isolate grouped with other CPMMV isolates from other plant species from Brazil, Ghana, India, Puerto Rico, Taiwan and USA, but were separated from four others from India. This is the first report of the presence of CPMMV in chia in the world. Although the other viruses were not detected in this work, it is possible that the different symptoms observed could be produced by a mixture of viruses because CPMMV was found in chia plants with different symptoms.
Inst. Patología Vegetal
Fil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Merino, Maria Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nome Docampo, Claudia Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Flores, Ceferino Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; Argentina
Fil: Conci, Vilma Cecilia Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Crop protection 89 :1-5. (November 2016)
Materia
Enfermedades de las Plantas
Salvia (género)
Virus de las Plantas
Plant Diseases
Plant Viruses
Chía
Salvia Hispánica
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1239

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oai_identifier_str oai:localhost:20.500.12123/1239
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling First report of cowpea mild mottle virus in chia (Salvia hispanica)Celli, Marcos GiovaniPerotto, Maria CeciliaMerino, Maria AliciaNome Docampo, ClaudiaFlores, Ceferino ReneConci, Vilma CeciliaEnfermedades de las PlantasSalvia (género)Virus de las PlantasPlant DiseasesPlant VirusesChíaSalvia HispánicaChia (Salvia hispanica), a herbaceous plant of Lamiaceae family, has gained popularity due to its high concentrations of health-beneficial Omega-3 fatty acids. Plants showing different virus-like symptoms were observed in the principal chia production areas in Argentina. Some plants exhibited yellowing, mottled and blistering leaves, and others shortened internodes and leaf and stem deformation. The aim of this study was to identify the viruses infecting this crop. Forty symptomatic chia plants were collected from nine production fields in northeastern Argentina. The samples were tested for Alfalfa mosaic virus (AMV), Cowpea mild mottle virus (CPMMV), Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV) and Tospovirus group (I, II and III) by DAS-ELISA, for the genera Potyvirus by PTA-ELISA and for Begomovirus by PCR. CPMMV was detected in three plants with different kind of symptoms. In these plants, feather-like inclusions formed by virions were observed with transmission electron microscopy. ORF2 to ORF6 (2462 nucleotides) from the CPMMV viral genome was amplified by RT-PCR. Nucleotide (nt) sequence of the coat-protein gene (CP – ORF5) of the chia virus isolate was obtained and compared with 31 CPMMV sequences reported in GenBank. Results showed between 75.5% and 99.0% of nt identity, which is above the International Committee on Taxonomy of Viruses criteria for Carlavirus species differentiation. The phylogenetic analysis with the CP gene nt sequences revealed that the CPMMV chia isolate grouped with other CPMMV isolates from other plant species from Brazil, Ghana, India, Puerto Rico, Taiwan and USA, but were separated from four others from India. This is the first report of the presence of CPMMV in chia in the world. Although the other viruses were not detected in this work, it is possible that the different symptoms observed could be produced by a mixture of viruses because CPMMV was found in chia plants with different symptoms.Inst. Patología VegetalFil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Merino, Maria Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Nome Docampo, Claudia Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; ArgentinaFil: Flores, Ceferino Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; ArgentinaFil: Conci, Vilma Cecilia Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina2017-09-18T14:34:09Z2017-09-18T14:34:09Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1239http://www.sciencedirect.com/science/article/pii/S02612194163014540261-2194https://doi.org/10.1016/j.cropro.2016.05.014Crop protection 89 :1-5. (November 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-04T09:47:01Zoai:localhost:20.500.12123/1239instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-04 09:47:03.275INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv First report of cowpea mild mottle virus in chia (Salvia hispanica)
title First report of cowpea mild mottle virus in chia (Salvia hispanica)
spellingShingle First report of cowpea mild mottle virus in chia (Salvia hispanica)
Celli, Marcos Giovani
Enfermedades de las Plantas
Salvia (género)
Virus de las Plantas
Plant Diseases
Plant Viruses
Chía
Salvia Hispánica
title_short First report of cowpea mild mottle virus in chia (Salvia hispanica)
title_full First report of cowpea mild mottle virus in chia (Salvia hispanica)
title_fullStr First report of cowpea mild mottle virus in chia (Salvia hispanica)
title_full_unstemmed First report of cowpea mild mottle virus in chia (Salvia hispanica)
title_sort First report of cowpea mild mottle virus in chia (Salvia hispanica)
dc.creator.none.fl_str_mv Celli, Marcos Giovani
Perotto, Maria Cecilia
Merino, Maria Alicia
Nome Docampo, Claudia
Flores, Ceferino Rene
Conci, Vilma Cecilia
author Celli, Marcos Giovani
author_facet Celli, Marcos Giovani
Perotto, Maria Cecilia
Merino, Maria Alicia
Nome Docampo, Claudia
Flores, Ceferino Rene
Conci, Vilma Cecilia
author_role author
author2 Perotto, Maria Cecilia
Merino, Maria Alicia
Nome Docampo, Claudia
Flores, Ceferino Rene
Conci, Vilma Cecilia
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de las Plantas
Salvia (género)
Virus de las Plantas
Plant Diseases
Plant Viruses
Chía
Salvia Hispánica
topic Enfermedades de las Plantas
Salvia (género)
Virus de las Plantas
Plant Diseases
Plant Viruses
Chía
Salvia Hispánica
dc.description.none.fl_txt_mv Chia (Salvia hispanica), a herbaceous plant of Lamiaceae family, has gained popularity due to its high concentrations of health-beneficial Omega-3 fatty acids. Plants showing different virus-like symptoms were observed in the principal chia production areas in Argentina. Some plants exhibited yellowing, mottled and blistering leaves, and others shortened internodes and leaf and stem deformation. The aim of this study was to identify the viruses infecting this crop. Forty symptomatic chia plants were collected from nine production fields in northeastern Argentina. The samples were tested for Alfalfa mosaic virus (AMV), Cowpea mild mottle virus (CPMMV), Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV) and Tospovirus group (I, II and III) by DAS-ELISA, for the genera Potyvirus by PTA-ELISA and for Begomovirus by PCR. CPMMV was detected in three plants with different kind of symptoms. In these plants, feather-like inclusions formed by virions were observed with transmission electron microscopy. ORF2 to ORF6 (2462 nucleotides) from the CPMMV viral genome was amplified by RT-PCR. Nucleotide (nt) sequence of the coat-protein gene (CP – ORF5) of the chia virus isolate was obtained and compared with 31 CPMMV sequences reported in GenBank. Results showed between 75.5% and 99.0% of nt identity, which is above the International Committee on Taxonomy of Viruses criteria for Carlavirus species differentiation. The phylogenetic analysis with the CP gene nt sequences revealed that the CPMMV chia isolate grouped with other CPMMV isolates from other plant species from Brazil, Ghana, India, Puerto Rico, Taiwan and USA, but were separated from four others from India. This is the first report of the presence of CPMMV in chia in the world. Although the other viruses were not detected in this work, it is possible that the different symptoms observed could be produced by a mixture of viruses because CPMMV was found in chia plants with different symptoms.
Inst. Patología Vegetal
Fil: Celli, Marcos Giovani. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Perotto, Maria Cecilia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Merino, Maria Alicia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Nome Docampo, Claudia Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina
Fil: Flores, Ceferino Rene. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Yuto; Argentina
Fil: Conci, Vilma Cecilia Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patología Vegetal; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Chia (Salvia hispanica), a herbaceous plant of Lamiaceae family, has gained popularity due to its high concentrations of health-beneficial Omega-3 fatty acids. Plants showing different virus-like symptoms were observed in the principal chia production areas in Argentina. Some plants exhibited yellowing, mottled and blistering leaves, and others shortened internodes and leaf and stem deformation. The aim of this study was to identify the viruses infecting this crop. Forty symptomatic chia plants were collected from nine production fields in northeastern Argentina. The samples were tested for Alfalfa mosaic virus (AMV), Cowpea mild mottle virus (CPMMV), Cucumber mosaic virus (CMV), Tobacco mosaic virus (TMV) and Tospovirus group (I, II and III) by DAS-ELISA, for the genera Potyvirus by PTA-ELISA and for Begomovirus by PCR. CPMMV was detected in three plants with different kind of symptoms. In these plants, feather-like inclusions formed by virions were observed with transmission electron microscopy. ORF2 to ORF6 (2462 nucleotides) from the CPMMV viral genome was amplified by RT-PCR. Nucleotide (nt) sequence of the coat-protein gene (CP – ORF5) of the chia virus isolate was obtained and compared with 31 CPMMV sequences reported in GenBank. Results showed between 75.5% and 99.0% of nt identity, which is above the International Committee on Taxonomy of Viruses criteria for Carlavirus species differentiation. The phylogenetic analysis with the CP gene nt sequences revealed that the CPMMV chia isolate grouped with other CPMMV isolates from other plant species from Brazil, Ghana, India, Puerto Rico, Taiwan and USA, but were separated from four others from India. This is the first report of the presence of CPMMV in chia in the world. Although the other viruses were not detected in this work, it is possible that the different symptoms observed could be produced by a mixture of viruses because CPMMV was found in chia plants with different symptoms.
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-09-18T14:34:09Z
2017-09-18T14:34:09Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1239
http://www.sciencedirect.com/science/article/pii/S0261219416301454
0261-2194
https://doi.org/10.1016/j.cropro.2016.05.014
url http://hdl.handle.net/20.500.12123/1239
http://www.sciencedirect.com/science/article/pii/S0261219416301454
https://doi.org/10.1016/j.cropro.2016.05.014
identifier_str_mv 0261-2194
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
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dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Crop protection 89 :1-5. (November 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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