Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends

Autores
Shimizu, Ernesto; Macias, Analía; Paolicchi, Fernando; Magnano, Gabriel; Zapata, Laura; Fernandez, Analía; Canal, Ana María; Garbaccio, Sergio Gabriel; Cataldi, Angel Adrian; Caimi, Karina Cynthia; Zumarraga, Martin Jose
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovis spoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism.
Instituto de Patobiología
Fil: Shimizu, Ernesto. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Macias, Analía. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Magnano, Gabriel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Zapata, Laura. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Fernandez, Analía. Ministerio de la Producción. Dirección General de Ganadería y Avicultura. Laboratorio de Sanidad Animal; Argentina
Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Patología Básica; Argentina
Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fuente
Memorias do Instituto Oswaldo Cruz 109 (2) : 236-245 (Abril 2014)
Materia
Mycobacterium bovis
Tuberculosis
Ganado Bovino
Tendencias
Genotipos
Buenos Aires
Cordoba (Argentina)
Santa Fe (Argentina)
Cattle
Trends
Genotypes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/8221
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oai_identifier_str oai:localhost:20.500.12123/8221
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network_name_str INTA Digital (INTA)
spelling Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trendsShimizu, ErnestoMacias, AnalíaPaolicchi, FernandoMagnano, GabrielZapata, LauraFernandez, AnalíaCanal, Ana MaríaGarbaccio, Sergio GabrielCataldi, Angel AdrianCaimi, Karina CynthiaZumarraga, Martin JoseMycobacterium bovisTuberculosisGanado BovinoTendenciasGenotiposBuenos AiresCordoba (Argentina)Santa Fe (Argentina)CattleTrendsGenotypesMycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovis spoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism.Instituto de PatobiologíaFil: Shimizu, Ernesto. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Macias, Analía. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; ArgentinaFil: Magnano, Gabriel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Zapata, Laura. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Fernandez, Analía. Ministerio de la Producción. Dirección General de Ganadería y Avicultura. Laboratorio de Sanidad Animal; ArgentinaFil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Patología Básica; ArgentinaFil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFundacao Oswaldo Cruz, Brasil2020-11-09T16:59:02Z2020-11-09T16:59:02Z2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8221https://memorias.ioc.fiocruz.br/article/1641/0292_genotyping-mycobacterium-bovis-from-cattle-in-the-central-pampas-of-argentina-temporal-and-regional-trends1678-80600074-0276http://dx.doi.org/10.1590/0074-0276140292Memorias do Instituto Oswaldo Cruz 109 (2) : 236-245 (Abril 2014)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-23T11:17:24Zoai:localhost:20.500.12123/8221instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-23 11:17:25.35INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends
title Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends
spellingShingle Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends
Shimizu, Ernesto
Mycobacterium bovis
Tuberculosis
Ganado Bovino
Tendencias
Genotipos
Buenos Aires
Cordoba (Argentina)
Santa Fe (Argentina)
Cattle
Trends
Genotypes
title_short Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends
title_full Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends
title_fullStr Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends
title_full_unstemmed Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends
title_sort Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: tempoal and regional trends
dc.creator.none.fl_str_mv Shimizu, Ernesto
Macias, Analía
Paolicchi, Fernando
Magnano, Gabriel
Zapata, Laura
Fernandez, Analía
Canal, Ana María
Garbaccio, Sergio Gabriel
Cataldi, Angel Adrian
Caimi, Karina Cynthia
Zumarraga, Martin Jose
author Shimizu, Ernesto
author_facet Shimizu, Ernesto
Macias, Analía
Paolicchi, Fernando
Magnano, Gabriel
Zapata, Laura
Fernandez, Analía
Canal, Ana María
Garbaccio, Sergio Gabriel
Cataldi, Angel Adrian
Caimi, Karina Cynthia
Zumarraga, Martin Jose
author_role author
author2 Macias, Analía
Paolicchi, Fernando
Magnano, Gabriel
Zapata, Laura
Fernandez, Analía
Canal, Ana María
Garbaccio, Sergio Gabriel
Cataldi, Angel Adrian
Caimi, Karina Cynthia
Zumarraga, Martin Jose
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mycobacterium bovis
Tuberculosis
Ganado Bovino
Tendencias
Genotipos
Buenos Aires
Cordoba (Argentina)
Santa Fe (Argentina)
Cattle
Trends
Genotypes
topic Mycobacterium bovis
Tuberculosis
Ganado Bovino
Tendencias
Genotipos
Buenos Aires
Cordoba (Argentina)
Santa Fe (Argentina)
Cattle
Trends
Genotypes
dc.description.none.fl_txt_mv Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovis spoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism.
Instituto de Patobiología
Fil: Shimizu, Ernesto. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Macias, Analía. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Paolicchi, Fernando. Universidad Nacional de Mar del Plata. Facultad de Ciencias Agrarias; Argentina
Fil: Magnano, Gabriel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Zapata, Laura. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Fernandez, Analía. Ministerio de la Producción. Dirección General de Ganadería y Avicultura. Laboratorio de Sanidad Animal; Argentina
Fil: Canal, Ana María. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Departamento de Patología Básica; Argentina
Fil: Garbaccio, Sergio Gabriel. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Cataldi, Angel Adrian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Caimi, Karina Cynthia. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
description Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovis spoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
2020-11-09T16:59:02Z
2020-11-09T16:59:02Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8221
https://memorias.ioc.fiocruz.br/article/1641/0292_genotyping-mycobacterium-bovis-from-cattle-in-the-central-pampas-of-argentina-temporal-and-regional-trends
1678-8060
0074-0276
http://dx.doi.org/10.1590/0074-0276140292
url http://hdl.handle.net/20.500.12123/8221
https://memorias.ioc.fiocruz.br/article/1641/0292_genotyping-mycobacterium-bovis-from-cattle-in-the-central-pampas-of-argentina-temporal-and-regional-trends
http://dx.doi.org/10.1590/0074-0276140292
identifier_str_mv 1678-8060
0074-0276
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Fundacao Oswaldo Cruz, Brasil
publisher.none.fl_str_mv Fundacao Oswaldo Cruz, Brasil
dc.source.none.fl_str_mv Memorias do Instituto Oswaldo Cruz 109 (2) : 236-245 (Abril 2014)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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