Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil

Autores
Carneiro, Paulo A. M.; Pasquatti, Taynara N.; Takatani, Haruo; Zumárraga, Martín José; Marfil, Maria Jimena; Barnard, Christian; Fitzgerald, Scott D.; Abramovitch, Robert B.; Araujo, Flábio R.; Kaneene, John B.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil.
Fil: Carneiro, Paulo A. M.. Michigan State University; Estados Unidos. Universidad Federal del Amazonas.; Brasil
Fil: Pasquatti, Taynara N.. Catholic University Dom Bosco; Brasil
Fil: Takatani, Haruo. Agencia de Defesa Agropecuaria do Amazonas; Brasil
Fil: Zumárraga, Martín José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Marfil, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Barnard, Christian. Agencia de Defesa Agropecuaria do Amazonas; Brasil
Fil: Fitzgerald, Scott D.. Michigan State University; Estados Unidos
Fil: Abramovitch, Robert B.. Michigan State University; Estados Unidos
Fil: Araujo, Flábio R.. Centro Nacional de Pesquisa de Gado de Corte; Brasil
Fil: Kaneene, John B.. Michigan State University; Estados Unidos
Materia
BOVINE TUBERCULOSIS
CATTLE AND BUFFALO
GENOTYPES
MYCOBACTERIUM BOVIS
SPOLIGOTYPING
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/163643
Acceder
id CONICETDig_39c6952ad1c76ed5b462587937122309
oai_identifier_str oai:ri.conicet.gov.ar:11336/163643
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, BrazilCarneiro, Paulo A. M.Pasquatti, Taynara N.Takatani, HaruoZumárraga, Martín JoséMarfil, Maria JimenaBarnard, ChristianFitzgerald, Scott D.Abramovitch, Robert B.Araujo, Flábio R.Kaneene, John B.BOVINE TUBERCULOSISCATTLE AND BUFFALOGENOTYPESMYCOBACTERIUM BOVISSPOLIGOTYPINGhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil.Fil: Carneiro, Paulo A. M.. Michigan State University; Estados Unidos. Universidad Federal del Amazonas.; BrasilFil: Pasquatti, Taynara N.. Catholic University Dom Bosco; BrasilFil: Takatani, Haruo. Agencia de Defesa Agropecuaria do Amazonas; BrasilFil: Zumárraga, Martín José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Marfil, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Barnard, Christian. Agencia de Defesa Agropecuaria do Amazonas; BrasilFil: Fitzgerald, Scott D.. Michigan State University; Estados UnidosFil: Abramovitch, Robert B.. Michigan State University; Estados UnidosFil: Araujo, Flábio R.. Centro Nacional de Pesquisa de Gado de Corte; BrasilFil: Kaneene, John B.. Michigan State University; Estados UnidosWiley Blackwell Publishing, Inc2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/163643Carneiro, Paulo A. M.; Pasquatti, Taynara N.; Takatani, Haruo; Zumárraga, Martín José; Marfil, Maria Jimena; et al.; Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil; Wiley Blackwell Publishing, Inc; Veterinary Medicine and Science; 6; 1; 2-2020; 133-1412053-1095CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/vms3.203info:eu-repo/semantics/altIdentifier/doi/10.1002/vms3.203info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:19:11Zoai:ri.conicet.gov.ar:11336/163643instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:19:11.34CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
title Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
spellingShingle Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
Carneiro, Paulo A. M.
BOVINE TUBERCULOSIS
CATTLE AND BUFFALO
GENOTYPES
MYCOBACTERIUM BOVIS
SPOLIGOTYPING
title_short Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
title_full Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
title_fullStr Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
title_full_unstemmed Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
title_sort Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
dc.creator.none.fl_str_mv Carneiro, Paulo A. M.
Pasquatti, Taynara N.
Takatani, Haruo
Zumárraga, Martín José
Marfil, Maria Jimena
Barnard, Christian
Fitzgerald, Scott D.
Abramovitch, Robert B.
Araujo, Flábio R.
Kaneene, John B.
author Carneiro, Paulo A. M.
author_facet Carneiro, Paulo A. M.
Pasquatti, Taynara N.
Takatani, Haruo
Zumárraga, Martín José
Marfil, Maria Jimena
Barnard, Christian
Fitzgerald, Scott D.
Abramovitch, Robert B.
Araujo, Flábio R.
Kaneene, John B.
author_role author
author2 Pasquatti, Taynara N.
Takatani, Haruo
Zumárraga, Martín José
Marfil, Maria Jimena
Barnard, Christian
Fitzgerald, Scott D.
Abramovitch, Robert B.
Araujo, Flábio R.
Kaneene, John B.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BOVINE TUBERCULOSIS
CATTLE AND BUFFALO
GENOTYPES
MYCOBACTERIUM BOVIS
SPOLIGOTYPING
topic BOVINE TUBERCULOSIS
CATTLE AND BUFFALO
GENOTYPES
MYCOBACTERIUM BOVIS
SPOLIGOTYPING
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil.
Fil: Carneiro, Paulo A. M.. Michigan State University; Estados Unidos. Universidad Federal del Amazonas.; Brasil
Fil: Pasquatti, Taynara N.. Catholic University Dom Bosco; Brasil
Fil: Takatani, Haruo. Agencia de Defesa Agropecuaria do Amazonas; Brasil
Fil: Zumárraga, Martín José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Marfil, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Barnard, Christian. Agencia de Defesa Agropecuaria do Amazonas; Brasil
Fil: Fitzgerald, Scott D.. Michigan State University; Estados Unidos
Fil: Abramovitch, Robert B.. Michigan State University; Estados Unidos
Fil: Araujo, Flábio R.. Centro Nacional de Pesquisa de Gado de Corte; Brasil
Fil: Kaneene, John B.. Michigan State University; Estados Unidos
description The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/163643
Carneiro, Paulo A. M.; Pasquatti, Taynara N.; Takatani, Haruo; Zumárraga, Martín José; Marfil, Maria Jimena; et al.; Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil; Wiley Blackwell Publishing, Inc; Veterinary Medicine and Science; 6; 1; 2-2020; 133-141
2053-1095
CONICET Digital
CONICET
url http://hdl.handle.net/11336/163643
identifier_str_mv Carneiro, Paulo A. M.; Pasquatti, Taynara N.; Takatani, Haruo; Zumárraga, Martín José; Marfil, Maria Jimena; et al.; Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil; Wiley Blackwell Publishing, Inc; Veterinary Medicine and Science; 6; 1; 2-2020; 133-141
2053-1095
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/url/https://onlinelibrary.wiley.com/doi/abs/10.1002/vms3.203
info:eu-repo/semantics/altIdentifier/doi/10.1002/vms3.203
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.069144

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