Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
- Autores
- Carneiro, Paulo A.M.; Pasquatti, Taynara N.; Takatani, Haruo; Zumarraga, Martin Jose; Marfil, Maria Jimena; Barnard, Christian; Fitzgerald, Scott D.; Abramovitch, Robert B.; Araujo, Flabio Ribeiro de; Kaneene, John B.
- Año de publicación
- 2020
- Idioma
- inglés
- Tipo de recurso
- artículo
- Estado
- versión publicada
- Descripción
- The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil.
Instituto de Biotecnología
Fil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidos
Fil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasil
Fil: Takatani, Haruo. Agencia de Defesa Agropecuaria do Amazonas; Brasil
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Barnard, Christian. Agencia de Defesa Agropecuaria do Amazonas; Brasil
Fil: Fitzgerald, Scott D. Michigan State University. Veterinary Diagnostic Laboratory; Estados Unidos
Fil: Abramovitch, Robert B. Michigan State University. Department of Microbiology and Molecular Genetics; Estados Unidos
Fil: Araujo, Flabio Ribeiro de. Centro Nacional de Pesquisa de Gado de Corte; Brasil
Fil: Kaneene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidos - Fuente
- Veterinary Medicine and Science 6 (1) : 133-141 (Febrero 2020)
- Materia
-
Mycobacterium bovis
Tuberculosis
Ganado Bovino
Razas de Búfalo
Genotipos
Brasil
Cattle
Buffalo Breeds
Genotypes - Nivel de accesibilidad
- acceso abierto
- Condiciones de uso
- http://creativecommons.org/licenses/by-nc-sa/4.0/
- Repositorio
- Institución
- Instituto Nacional de Tecnología Agropecuaria
- OAI Identificador
- oai:localhost:20.500.12123/8553
Ver los metadatos del registro completo
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Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, BrazilCarneiro, Paulo A.M.Pasquatti, Taynara N.Takatani, HaruoZumarraga, Martin JoseMarfil, Maria JimenaBarnard, ChristianFitzgerald, Scott D.Abramovitch, Robert B.Araujo, Flabio Ribeiro deKaneene, John B.Mycobacterium bovisTuberculosisGanado BovinoRazas de BúfaloGenotiposBrasilCattleBuffalo BreedsGenotypesThe aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil.Instituto de BiotecnologíaFil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados UnidosFil: Pasquatti, Taynara N. Dom Bosco Catholic University; BrasilFil: Takatani, Haruo. Agencia de Defesa Agropecuaria do Amazonas; BrasilFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Barnard, Christian. Agencia de Defesa Agropecuaria do Amazonas; BrasilFil: Fitzgerald, Scott D. Michigan State University. Veterinary Diagnostic Laboratory; Estados UnidosFil: Abramovitch, Robert B. Michigan State University. Department of Microbiology and Molecular Genetics; Estados UnidosFil: Araujo, Flabio Ribeiro de. Centro Nacional de Pesquisa de Gado de Corte; BrasilFil: Kaneene, John B. Michigan State University. Center for Comparative Epidemiology; Estados UnidosWiley2021-01-05T17:58:19Z2021-01-05T17:58:19Z2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8553https://onlinelibrary.wiley.com/doi/full/10.1002/vms3.2032053-1095https://doi.org/10.1002/vms3.203Veterinary Medicine and Science 6 (1) : 133-141 (Febrero 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:59Zoai:localhost:20.500.12123/8553instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:59.398INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse |
dc.title.none.fl_str_mv |
Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil |
title |
Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil |
spellingShingle |
Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil Carneiro, Paulo A.M. Mycobacterium bovis Tuberculosis Ganado Bovino Razas de Búfalo Genotipos Brasil Cattle Buffalo Breeds Genotypes |
title_short |
Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil |
title_full |
Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil |
title_fullStr |
Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil |
title_full_unstemmed |
Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil |
title_sort |
Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil |
dc.creator.none.fl_str_mv |
Carneiro, Paulo A.M. Pasquatti, Taynara N. Takatani, Haruo Zumarraga, Martin Jose Marfil, Maria Jimena Barnard, Christian Fitzgerald, Scott D. Abramovitch, Robert B. Araujo, Flabio Ribeiro de Kaneene, John B. |
author |
Carneiro, Paulo A.M. |
author_facet |
Carneiro, Paulo A.M. Pasquatti, Taynara N. Takatani, Haruo Zumarraga, Martin Jose Marfil, Maria Jimena Barnard, Christian Fitzgerald, Scott D. Abramovitch, Robert B. Araujo, Flabio Ribeiro de Kaneene, John B. |
author_role |
author |
author2 |
Pasquatti, Taynara N. Takatani, Haruo Zumarraga, Martin Jose Marfil, Maria Jimena Barnard, Christian Fitzgerald, Scott D. Abramovitch, Robert B. Araujo, Flabio Ribeiro de Kaneene, John B. |
author2_role |
author author author author author author author author author |
dc.subject.none.fl_str_mv |
Mycobacterium bovis Tuberculosis Ganado Bovino Razas de Búfalo Genotipos Brasil Cattle Buffalo Breeds Genotypes |
topic |
Mycobacterium bovis Tuberculosis Ganado Bovino Razas de Búfalo Genotipos Brasil Cattle Buffalo Breeds Genotypes |
dc.description.none.fl_txt_mv |
The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil. Instituto de Biotecnología Fil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidos Fil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasil Fil: Takatani, Haruo. Agencia de Defesa Agropecuaria do Amazonas; Brasil Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina Fil: Barnard, Christian. Agencia de Defesa Agropecuaria do Amazonas; Brasil Fil: Fitzgerald, Scott D. Michigan State University. Veterinary Diagnostic Laboratory; Estados Unidos Fil: Abramovitch, Robert B. Michigan State University. Department of Microbiology and Molecular Genetics; Estados Unidos Fil: Araujo, Flabio Ribeiro de. Centro Nacional de Pesquisa de Gado de Corte; Brasil Fil: Kaneene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidos |
description |
The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-02 2021-01-05T17:58:19Z 2021-01-05T17:58:19Z |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion http://purl.org/coar/resource_type/c_6501 info:ar-repo/semantics/articulo |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
http://hdl.handle.net/20.500.12123/8553 https://onlinelibrary.wiley.com/doi/full/10.1002/vms3.203 2053-1095 https://doi.org/10.1002/vms3.203 |
url |
http://hdl.handle.net/20.500.12123/8553 https://onlinelibrary.wiley.com/doi/full/10.1002/vms3.203 https://doi.org/10.1002/vms3.203 |
identifier_str_mv |
2053-1095 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
eu_rights_str_mv |
openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc-sa/4.0/ Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0) |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Wiley |
publisher.none.fl_str_mv |
Wiley |
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Veterinary Medicine and Science 6 (1) : 133-141 (Febrero 2020) reponame:INTA Digital (INTA) instname:Instituto Nacional de Tecnología Agropecuaria |
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