Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil

Autores
Carneiro, Paulo A.M.; Pasquatti, Taynara N.; Takatani, Haruo; Zumarraga, Martin Jose; Marfil, Maria Jimena; Barnard, Christian; Fitzgerald, Scott D.; Abramovitch, Robert B.; Araujo, Flabio Ribeiro de; Kaneene, John B.
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil.
Instituto de Biotecnología
Fil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidos
Fil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasil
Fil: Takatani, Haruo. Agencia de Defesa Agropecuaria do Amazonas; Brasil
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Barnard, Christian. Agencia de Defesa Agropecuaria do Amazonas; Brasil
Fil: Fitzgerald, Scott D. Michigan State University. Veterinary Diagnostic Laboratory; Estados Unidos
Fil: Abramovitch, Robert B. Michigan State University. Department of Microbiology and Molecular Genetics; Estados Unidos
Fil: Araujo, Flabio Ribeiro de. Centro Nacional de Pesquisa de Gado de Corte; Brasil
Fil: Kaneene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidos
Fuente
Veterinary Medicine and Science 6 (1) : 133-141 (Febrero 2020)
Materia
Mycobacterium bovis
Tuberculosis
Ganado Bovino
Razas de Búfalo
Genotipos
Brasil
Cattle
Buffalo Breeds
Genotypes
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
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network_name_str INTA Digital (INTA)
spelling Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, BrazilCarneiro, Paulo A.M.Pasquatti, Taynara N.Takatani, HaruoZumarraga, Martin JoseMarfil, Maria JimenaBarnard, ChristianFitzgerald, Scott D.Abramovitch, Robert B.Araujo, Flabio Ribeiro deKaneene, John B.Mycobacterium bovisTuberculosisGanado BovinoRazas de BúfaloGenotiposBrasilCattleBuffalo BreedsGenotypesThe aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil.Instituto de BiotecnologíaFil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados UnidosFil: Pasquatti, Taynara N. Dom Bosco Catholic University; BrasilFil: Takatani, Haruo. Agencia de Defesa Agropecuaria do Amazonas; BrasilFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; ArgentinaFil: Barnard, Christian. Agencia de Defesa Agropecuaria do Amazonas; BrasilFil: Fitzgerald, Scott D. Michigan State University. Veterinary Diagnostic Laboratory; Estados UnidosFil: Abramovitch, Robert B. Michigan State University. Department of Microbiology and Molecular Genetics; Estados UnidosFil: Araujo, Flabio Ribeiro de. Centro Nacional de Pesquisa de Gado de Corte; BrasilFil: Kaneene, John B. Michigan State University. Center for Comparative Epidemiology; Estados UnidosWiley2021-01-05T17:58:19Z2021-01-05T17:58:19Z2020-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/8553https://onlinelibrary.wiley.com/doi/full/10.1002/vms3.2032053-1095https://doi.org/10.1002/vms3.203Veterinary Medicine and Science 6 (1) : 133-141 (Febrero 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-10-16T09:29:59Zoai:localhost:20.500.12123/8553instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:29:59.398INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
title Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
spellingShingle Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
Carneiro, Paulo A.M.
Mycobacterium bovis
Tuberculosis
Ganado Bovino
Razas de Búfalo
Genotipos
Brasil
Cattle
Buffalo Breeds
Genotypes
title_short Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
title_full Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
title_fullStr Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
title_full_unstemmed Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
title_sort Molecular characterization of Mycobacterium bovis infection in cattle and buffalo in Amazon Region, Brazil
dc.creator.none.fl_str_mv Carneiro, Paulo A.M.
Pasquatti, Taynara N.
Takatani, Haruo
Zumarraga, Martin Jose
Marfil, Maria Jimena
Barnard, Christian
Fitzgerald, Scott D.
Abramovitch, Robert B.
Araujo, Flabio Ribeiro de
Kaneene, John B.
author Carneiro, Paulo A.M.
author_facet Carneiro, Paulo A.M.
Pasquatti, Taynara N.
Takatani, Haruo
Zumarraga, Martin Jose
Marfil, Maria Jimena
Barnard, Christian
Fitzgerald, Scott D.
Abramovitch, Robert B.
Araujo, Flabio Ribeiro de
Kaneene, John B.
author_role author
author2 Pasquatti, Taynara N.
Takatani, Haruo
Zumarraga, Martin Jose
Marfil, Maria Jimena
Barnard, Christian
Fitzgerald, Scott D.
Abramovitch, Robert B.
Araujo, Flabio Ribeiro de
Kaneene, John B.
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Mycobacterium bovis
Tuberculosis
Ganado Bovino
Razas de Búfalo
Genotipos
Brasil
Cattle
Buffalo Breeds
Genotypes
topic Mycobacterium bovis
Tuberculosis
Ganado Bovino
Razas de Búfalo
Genotipos
Brasil
Cattle
Buffalo Breeds
Genotypes
dc.description.none.fl_txt_mv The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil.
Instituto de Biotecnología
Fil: Carneiro, Paulo A. M. Michigan State University. Center for Comparative Epidemiology; Estados Unidos
Fil: Pasquatti, Taynara N. Dom Bosco Catholic University; Brasil
Fil: Takatani, Haruo. Agencia de Defesa Agropecuaria do Amazonas; Brasil
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Biotecnología; Argentina
Fil: Barnard, Christian. Agencia de Defesa Agropecuaria do Amazonas; Brasil
Fil: Fitzgerald, Scott D. Michigan State University. Veterinary Diagnostic Laboratory; Estados Unidos
Fil: Abramovitch, Robert B. Michigan State University. Department of Microbiology and Molecular Genetics; Estados Unidos
Fil: Araujo, Flabio Ribeiro de. Centro Nacional de Pesquisa de Gado de Corte; Brasil
Fil: Kaneene, John B. Michigan State University. Center for Comparative Epidemiology; Estados Unidos
description The aim of this study was to characterize Mycobacterium bovis from cattle and buffalo tissue samples, from two Brazilian states, and to analyse their genetic diversity by spoligotyping. Tissue samples from tuberculosis suspect animals, 57 in Amazonas State (12 cattle and 45 buffaloes) and six from Pará State (5 cattle and one buffalo) from slaughterhouses under State Veterinary Inspection, were isolated in culture medium Stonebrink. The positive cultures were confirmed by PCR and analysed by the spoligotyping technique and the patterns (spoligotypes) were identified and compared at the Mycobacterium bovis Spoligotype Database (http://www.mbovis.org/). There was bacterial growth in 44 (69.8%) of the tissues of the 63 animals, of which PCR for region of differentiation 4 identified 35/44 (79.5%) as Mycobacterium bovis. Six different spoligotypes were identified among the 35 Mycobacterium bovis isolates, of which SB0295, SB1869, SB0121 and SB1800 had already been described in Brazil, and SB0822 and SB1608 had not been described. The most frequent spoligotype in this study (SB0822) had already been described in buffaloes in Colombia, a neighbouring country of Amazonas state. The other identified spoligotypes were also described in other South American countries, such as Argentina and Venezuela, and described in the Brazilian states of Rio Grande do Sul, Santa Catarina, São Paulo, Minas Gerais, Mato Grosso do Sul, Mato Grosso and Goiás, indicating an active movement of Mycobacterium bovis strains within Brazil.
publishDate 2020
dc.date.none.fl_str_mv 2020-02
2021-01-05T17:58:19Z
2021-01-05T17:58:19Z
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dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/8553
https://onlinelibrary.wiley.com/doi/full/10.1002/vms3.203
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https://doi.org/10.1002/vms3.203
url http://hdl.handle.net/20.500.12123/8553
https://onlinelibrary.wiley.com/doi/full/10.1002/vms3.203
https://doi.org/10.1002/vms3.203
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rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv Veterinary Medicine and Science 6 (1) : 133-141 (Febrero 2020)
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