Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil

Autores
Souza, Mariana Assunção de; Lopes, Thaís Aline Carolino; Silva, Brendhal Almeida; Bombonato, Nadia Grandi; Dib, Cristina Corsi; Marfil, Maria Jimena; Zumarraga, Martin Jose; Lima, Anna Monteiro Correia
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Genotyping methods have led to a better understanding of the epidemiology of Mycobacterium bovis (M. bovis) infection, and its transmission dynamics, as well as the possible phylogenetic relationships between Mycobacterium strains, thus making bovine tuberculosis control programs more efficient. The goal of this study was to characterize the main spoligotypes of M. bovis isolated from cattle in the State of Minas Gerais, Brazil. It was carried out in 28 municipalities of “Triângulo Mineiro” and “Alto Paranaíba” regions of the state. Viscera samples were obtained from 58 bovines positive for tuberculosis according to comparative cervical tests, and from another 100 bovines with lesions suggestive of tuberculosis, which were donated by the National Agricultural Laboratory of Pedro Leopoldo, Minas Gerais. Microbiological isolation was performed in Stonebrink medium, and molecular identification of mycobacteria was performed by PCR. Genotyping was performed using the spoligotyping method at the Agrobiotechnology and Molecular Biology Institute of National Agricultural Technology Institute-National Scientific and Technical Research Council, Buenos Aires, Argentina. Among the 158 viscera samples, we obtained 40 (25%) isolates of M. bovis, and detected 11 spoligotype patterns, with a predominance of SB1142 (37.5%), SB0121 (25.0%), and SB1145 (10.0%). Other standards, SB0295, SB1050, SB0881, SB1144, SB1802, SB0140, SB0120, and SB0849, varied from 2.5 to 7.5%, heterogeneously distributed among the municipalities. The presence of spoligotypes shared with other Brazilian states and different countries indicates their possible exchange through epidemiological relationships, such as the transit of live animals and/or genetic similarity between strains that share a common ancestor.
Instituto de Biotecnología
Fil: de Souza, Mariana Assunção. Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Laboratório de Doenças Infectocontagiosas; Brasil
Fil: Lopes, Thaís Aline Carolino. Centro Universitário de Patos de Minas. Faculdade de Medicina Veterinária; Brasil
Fil: Silva, Brendhal Almeida. Centro Universitário de Patos de Minas. Faculdade de Medicina Veterinária; Brasil
Fil: Bombonato, Nadia Grandi. Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Laboratório de Doenças Infectocontagiosas; Brasil
Fil: Dib, Cristina Corsi. Instituto Biológico de São Paulo. Laboratório de Tuberculose; Brasil
Fil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Marfil, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lima, Anna Monteiro Correia. Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Laboratório de Doenças Infectocontagiosas; Brasil
Fuente
Tropical Animal Health and Production 54 (4) : 238 (Julio 2022)
Materia
Epidemiology
Tuberculosis
Cattle
Genotyping
Brazil
Epidemiología
Bovinae
Mycobacterium bovis
Ganado Bovino
Genotipado
Brasil
Spoligotypes
Espoligotipos
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/12493
Acceder
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oai_identifier_str oai:localhost:20.500.12123/12493
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network_name_str INTA Digital (INTA)
spelling Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, BrazilSouza, Mariana Assunção deLopes, Thaís Aline CarolinoSilva, Brendhal AlmeidaBombonato, Nadia GrandiDib, Cristina CorsiMarfil, Maria JimenaZumarraga, Martin JoseLima, Anna Monteiro CorreiaEpidemiologyTuberculosisCattleGenotypingBrazilEpidemiologíaBovinaeMycobacterium bovisGanado BovinoGenotipadoBrasilSpoligotypesEspoligotiposGenotyping methods have led to a better understanding of the epidemiology of Mycobacterium bovis (M. bovis) infection, and its transmission dynamics, as well as the possible phylogenetic relationships between Mycobacterium strains, thus making bovine tuberculosis control programs more efficient. The goal of this study was to characterize the main spoligotypes of M. bovis isolated from cattle in the State of Minas Gerais, Brazil. It was carried out in 28 municipalities of “Triângulo Mineiro” and “Alto Paranaíba” regions of the state. Viscera samples were obtained from 58 bovines positive for tuberculosis according to comparative cervical tests, and from another 100 bovines with lesions suggestive of tuberculosis, which were donated by the National Agricultural Laboratory of Pedro Leopoldo, Minas Gerais. Microbiological isolation was performed in Stonebrink medium, and molecular identification of mycobacteria was performed by PCR. Genotyping was performed using the spoligotyping method at the Agrobiotechnology and Molecular Biology Institute of National Agricultural Technology Institute-National Scientific and Technical Research Council, Buenos Aires, Argentina. Among the 158 viscera samples, we obtained 40 (25%) isolates of M. bovis, and detected 11 spoligotype patterns, with a predominance of SB1142 (37.5%), SB0121 (25.0%), and SB1145 (10.0%). Other standards, SB0295, SB1050, SB0881, SB1144, SB1802, SB0140, SB0120, and SB0849, varied from 2.5 to 7.5%, heterogeneously distributed among the municipalities. The presence of spoligotypes shared with other Brazilian states and different countries indicates their possible exchange through epidemiological relationships, such as the transit of live animals and/or genetic similarity between strains that share a common ancestor.Instituto de BiotecnologíaFil: de Souza, Mariana Assunção. Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Laboratório de Doenças Infectocontagiosas; BrasilFil: Lopes, Thaís Aline Carolino. Centro Universitário de Patos de Minas. Faculdade de Medicina Veterinária; BrasilFil: Silva, Brendhal Almeida. Centro Universitário de Patos de Minas. Faculdade de Medicina Veterinária; BrasilFil: Bombonato, Nadia Grandi. Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Laboratório de Doenças Infectocontagiosas; BrasilFil: Dib, Cristina Corsi. Instituto Biológico de São Paulo. Laboratório de Tuberculose; BrasilFil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Marfil, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; ArgentinaFil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Lima, Anna Monteiro Correia. Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Laboratório de Doenças Infectocontagiosas; BrasilSpringer2022-08-04T11:29:23Z2022-08-04T11:29:23Z2022-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12493https://link.springer.com/article/10.1007/s11250-022-03243-21573-7438https://doi.org/10.1007/s11250-022-03243-2Tropical Animal Health and Production 54 (4) : 238 (Julio 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PD-E5-I103-001/2019-PD-E5-I103-001/AR./Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud públicainfo:eu-repo/semantics/restrictedAccess2025-10-16T09:30:50Zoai:localhost:20.500.12123/12493instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:30:50.523INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil
title Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil
spellingShingle Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil
Souza, Mariana Assunção de
Epidemiology
Tuberculosis
Cattle
Genotyping
Brazil
Epidemiología
Bovinae
Mycobacterium bovis
Ganado Bovino
Genotipado
Brasil
Spoligotypes
Espoligotipos
title_short Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil
title_full Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil
title_fullStr Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil
title_full_unstemmed Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil
title_sort Spatial distribution of Mycobacterium bovis spoligotypes in cattle from the state of Minas Gerais, Brazil
dc.creator.none.fl_str_mv Souza, Mariana Assunção de
Lopes, Thaís Aline Carolino
Silva, Brendhal Almeida
Bombonato, Nadia Grandi
Dib, Cristina Corsi
Marfil, Maria Jimena
Zumarraga, Martin Jose
Lima, Anna Monteiro Correia
author Souza, Mariana Assunção de
author_facet Souza, Mariana Assunção de
Lopes, Thaís Aline Carolino
Silva, Brendhal Almeida
Bombonato, Nadia Grandi
Dib, Cristina Corsi
Marfil, Maria Jimena
Zumarraga, Martin Jose
Lima, Anna Monteiro Correia
author_role author
author2 Lopes, Thaís Aline Carolino
Silva, Brendhal Almeida
Bombonato, Nadia Grandi
Dib, Cristina Corsi
Marfil, Maria Jimena
Zumarraga, Martin Jose
Lima, Anna Monteiro Correia
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Epidemiology
Tuberculosis
Cattle
Genotyping
Brazil
Epidemiología
Bovinae
Mycobacterium bovis
Ganado Bovino
Genotipado
Brasil
Spoligotypes
Espoligotipos
topic Epidemiology
Tuberculosis
Cattle
Genotyping
Brazil
Epidemiología
Bovinae
Mycobacterium bovis
Ganado Bovino
Genotipado
Brasil
Spoligotypes
Espoligotipos
dc.description.none.fl_txt_mv Genotyping methods have led to a better understanding of the epidemiology of Mycobacterium bovis (M. bovis) infection, and its transmission dynamics, as well as the possible phylogenetic relationships between Mycobacterium strains, thus making bovine tuberculosis control programs more efficient. The goal of this study was to characterize the main spoligotypes of M. bovis isolated from cattle in the State of Minas Gerais, Brazil. It was carried out in 28 municipalities of “Triângulo Mineiro” and “Alto Paranaíba” regions of the state. Viscera samples were obtained from 58 bovines positive for tuberculosis according to comparative cervical tests, and from another 100 bovines with lesions suggestive of tuberculosis, which were donated by the National Agricultural Laboratory of Pedro Leopoldo, Minas Gerais. Microbiological isolation was performed in Stonebrink medium, and molecular identification of mycobacteria was performed by PCR. Genotyping was performed using the spoligotyping method at the Agrobiotechnology and Molecular Biology Institute of National Agricultural Technology Institute-National Scientific and Technical Research Council, Buenos Aires, Argentina. Among the 158 viscera samples, we obtained 40 (25%) isolates of M. bovis, and detected 11 spoligotype patterns, with a predominance of SB1142 (37.5%), SB0121 (25.0%), and SB1145 (10.0%). Other standards, SB0295, SB1050, SB0881, SB1144, SB1802, SB0140, SB0120, and SB0849, varied from 2.5 to 7.5%, heterogeneously distributed among the municipalities. The presence of spoligotypes shared with other Brazilian states and different countries indicates their possible exchange through epidemiological relationships, such as the transit of live animals and/or genetic similarity between strains that share a common ancestor.
Instituto de Biotecnología
Fil: de Souza, Mariana Assunção. Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Laboratório de Doenças Infectocontagiosas; Brasil
Fil: Lopes, Thaís Aline Carolino. Centro Universitário de Patos de Minas. Faculdade de Medicina Veterinária; Brasil
Fil: Silva, Brendhal Almeida. Centro Universitário de Patos de Minas. Faculdade de Medicina Veterinária; Brasil
Fil: Bombonato, Nadia Grandi. Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Laboratório de Doenças Infectocontagiosas; Brasil
Fil: Dib, Cristina Corsi. Instituto Biológico de São Paulo. Laboratório de Tuberculose; Brasil
Fil: Marfil, Maria Jimena. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Marfil, Maria Jimena. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Zumarraga, Martin Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Agrobiotecnología y Biología Molecular; Argentina
Fil: Zumarraga, Martin Jose. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Lima, Anna Monteiro Correia. Universidade Federal de Uberlândia. Faculdade de Medicina Veterinária. Laboratório de Doenças Infectocontagiosas; Brasil
description Genotyping methods have led to a better understanding of the epidemiology of Mycobacterium bovis (M. bovis) infection, and its transmission dynamics, as well as the possible phylogenetic relationships between Mycobacterium strains, thus making bovine tuberculosis control programs more efficient. The goal of this study was to characterize the main spoligotypes of M. bovis isolated from cattle in the State of Minas Gerais, Brazil. It was carried out in 28 municipalities of “Triângulo Mineiro” and “Alto Paranaíba” regions of the state. Viscera samples were obtained from 58 bovines positive for tuberculosis according to comparative cervical tests, and from another 100 bovines with lesions suggestive of tuberculosis, which were donated by the National Agricultural Laboratory of Pedro Leopoldo, Minas Gerais. Microbiological isolation was performed in Stonebrink medium, and molecular identification of mycobacteria was performed by PCR. Genotyping was performed using the spoligotyping method at the Agrobiotechnology and Molecular Biology Institute of National Agricultural Technology Institute-National Scientific and Technical Research Council, Buenos Aires, Argentina. Among the 158 viscera samples, we obtained 40 (25%) isolates of M. bovis, and detected 11 spoligotype patterns, with a predominance of SB1142 (37.5%), SB0121 (25.0%), and SB1145 (10.0%). Other standards, SB0295, SB1050, SB0881, SB1144, SB1802, SB0140, SB0120, and SB0849, varied from 2.5 to 7.5%, heterogeneously distributed among the municipalities. The presence of spoligotypes shared with other Brazilian states and different countries indicates their possible exchange through epidemiological relationships, such as the transit of live animals and/or genetic similarity between strains that share a common ancestor.
publishDate 2022
dc.date.none.fl_str_mv 2022-08-04T11:29:23Z
2022-08-04T11:29:23Z
2022-07
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/12493
https://link.springer.com/article/10.1007/s11250-022-03243-2
1573-7438
https://doi.org/10.1007/s11250-022-03243-2
url http://hdl.handle.net/20.500.12123/12493
https://link.springer.com/article/10.1007/s11250-022-03243-2
https://doi.org/10.1007/s11250-022-03243-2
identifier_str_mv 1573-7438
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PD-E5-I103-001/2019-PD-E5-I103-001/AR./Desarrollo de tecnologías diagnósticas y estudios epidemiológicos para el control de enfermedades que afectan la producción animal y la salud pública
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Tropical Animal Health and Production 54 (4) : 238 (Julio 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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