Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends

Autores
Shimizu, Ernesto; Macías, Analía; Paolicchi, Fernando Alberto; Magnano, Gabriel; Zapata, Laura; Fernández, Analía; Canal, Ana; Garbaccio, Sergio; Cataldi, Ángel Adrián; Caimi, Karina Cynthia; Zumárraga, Martín José
Año de publicación
2014
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovisspoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism.
Fil: Shimizu, Ernesto. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
Fil: Macías, Analía. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Paolicchi, Fernando Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
Fil: Magnano, Gabriel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Zapata, Laura. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Fernández, Analía. Provincia de Entre Ríos. Secretaría de Producción. Dirección General de Producción Animal; Argentina
Fil: Canal, Ana. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Garbaccio, Sergio. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Cataldi, Ángel Adrián. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caimi, Karina Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Zumárraga, Martín José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Materia
Bovine TB
Mycobacterium bovis
Spoligotype
VNTRs
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/33394
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/33394
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trendsShimizu, ErnestoMacías, AnalíaPaolicchi, Fernando AlbertoMagnano, GabrielZapata, LauraFernández, AnalíaCanal, AnaGarbaccio, SergioCataldi, Ángel AdriánCaimi, Karina CynthiaZumárraga, Martín JoséBovine TBMycobacterium bovisSpoligotypeVNTRshttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovisspoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism.Fil: Shimizu, Ernesto. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; ArgentinaFil: Macías, Analía. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Paolicchi, Fernando Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; ArgentinaFil: Magnano, Gabriel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Zapata, Laura. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; ArgentinaFil: Fernández, Analía. Provincia de Entre Ríos. Secretaría de Producción. Dirección General de Producción Animal; ArgentinaFil: Canal, Ana. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; ArgentinaFil: Garbaccio, Sergio. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; ArgentinaFil: Cataldi, Ángel Adrián. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Caimi, Karina Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFil: Zumárraga, Martín José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; ArgentinaFundação Oswaldo Cruz2014-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/33394Cataldi, Ángel Adrián; Caimi, Karina Cynthia; Shimizu, Ernesto; Zumárraga, Martín José; Fernández, Analía; Magnano, Gabriel; et al.; Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 109; 2; 4-2014; 236-2450074-02761678-8060CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1590/0074-0276140292info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015263/info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/4fmzr7info:eu-repo/semantics/altIdentifier/url/http://memorias.ioc.fiocruz.br/issues/past-issues/item/1641-0292_genotyping-mycobacterium-bovis-from-cattle-in-the-central-pampas-of-argentina-temporal-and-regional-trendsinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-10-15T15:36:34Zoai:ri.conicet.gov.ar:11336/33394instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-10-15 15:36:35.267CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
title Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
spellingShingle Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
Shimizu, Ernesto
Bovine TB
Mycobacterium bovis
Spoligotype
VNTRs
title_short Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
title_full Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
title_fullStr Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
title_full_unstemmed Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
title_sort Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends
dc.creator.none.fl_str_mv Shimizu, Ernesto
Macías, Analía
Paolicchi, Fernando Alberto
Magnano, Gabriel
Zapata, Laura
Fernández, Analía
Canal, Ana
Garbaccio, Sergio
Cataldi, Ángel Adrián
Caimi, Karina Cynthia
Zumárraga, Martín José
author Shimizu, Ernesto
author_facet Shimizu, Ernesto
Macías, Analía
Paolicchi, Fernando Alberto
Magnano, Gabriel
Zapata, Laura
Fernández, Analía
Canal, Ana
Garbaccio, Sergio
Cataldi, Ángel Adrián
Caimi, Karina Cynthia
Zumárraga, Martín José
author_role author
author2 Macías, Analía
Paolicchi, Fernando Alberto
Magnano, Gabriel
Zapata, Laura
Fernández, Analía
Canal, Ana
Garbaccio, Sergio
Cataldi, Ángel Adrián
Caimi, Karina Cynthia
Zumárraga, Martín José
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Bovine TB
Mycobacterium bovis
Spoligotype
VNTRs
topic Bovine TB
Mycobacterium bovis
Spoligotype
VNTRs
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovisspoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism.
Fil: Shimizu, Ernesto. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
Fil: Macías, Analía. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Paolicchi, Fernando Alberto. Universidad Nacional de La Plata. Facultad de Ciencias Agrarias y Forestales; Argentina
Fil: Magnano, Gabriel. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Zapata, Laura. Universidad Nacional de Río Cuarto. Facultad de Agronomía y Veterinaria; Argentina
Fil: Fernández, Analía. Provincia de Entre Ríos. Secretaría de Producción. Dirección General de Producción Animal; Argentina
Fil: Canal, Ana. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina
Fil: Garbaccio, Sergio. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina
Fil: Cataldi, Ángel Adrián. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Caimi, Karina Cynthia. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
Fil: Zumárraga, Martín José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro Nacional de Investigaciones Agropecuarias Castelar. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Biotecnología; Argentina
description Mycobacterium bovis is the causative agent of bovine tuberculosis (TB), a disease that affects approximately 5% of Argentinean cattle. Among the molecular methods for genotyping, the most convenient are spoligotyping and variable number of tandem repeats (VNTR). A total of 378 samples from bovines with visible lesions consistent with TB were collected at slaughterhouses in three provinces, yielding 265 M. bovisspoligotyped isolates, which were distributed into 35 spoligotypes. In addition, 197 isolates were also typed by the VNTR method and 54 combined VNTR types were detected. There were 24 clusters and 27 orphan types. When both typing methods were combined, 98 spoligotypes and VNTR types were observed with 27 clusters and 71 orphan types. By performing a meta-analysis with previous spoligotyping results, we identified regional and temporal trends in the population structure of M. bovis. For SB0140, the most predominant spoligotype in Argentina, the prevalence percentage remained high during different periods, varying from 25.5-57.8% (1994-2011). By contrast, the second and third most prevalent spoligotypes exhibited important fluctuations. This study shows that there has been an expansion in ancestral lineages as demonstrated by spoligotyping. However, exact tandem repeat typing suggests dynamic changes in the clonal population of this microorganism.
publishDate 2014
dc.date.none.fl_str_mv 2014-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/33394
Cataldi, Ángel Adrián; Caimi, Karina Cynthia; Shimizu, Ernesto; Zumárraga, Martín José; Fernández, Analía; Magnano, Gabriel; et al.; Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 109; 2; 4-2014; 236-245
0074-0276
1678-8060
CONICET Digital
CONICET
url http://hdl.handle.net/11336/33394
identifier_str_mv Cataldi, Ángel Adrián; Caimi, Karina Cynthia; Shimizu, Ernesto; Zumárraga, Martín José; Fernández, Analía; Magnano, Gabriel; et al.; Genotyping Mycobacterium bovis from cattle in the Central Pampas of Argentina: temporal and regional trends; Fundação Oswaldo Cruz; Memórias do Instituto Oswaldo Cruz; 109; 2; 4-2014; 236-245
0074-0276
1678-8060
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1590/0074-0276140292
info:eu-repo/semantics/altIdentifier/url/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4015263/
info:eu-repo/semantics/altIdentifier/url/http://ref.scielo.org/4fmzr7
info:eu-repo/semantics/altIdentifier/url/http://memorias.ioc.fiocruz.br/issues/past-issues/item/1641-0292_genotyping-mycobacterium-bovis-from-cattle-in-the-central-pampas-of-argentina-temporal-and-regional-trends
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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