In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses

Autores
Bustos, Carla Paola; Leiva, Carlos Leónidas; Gambarotta, Mariana; Guida, Nora; Chacana, Pablo
Año de publicación
2021
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Equine salmonellosis is caused by several Salmonella serotypes, including Salmonella Newport, which cause enterocolitis and diarrhea. Treatment usually includes the administration of antibiotics. However, since multidrug-resistant Salmonella is commonly detected, alternative options to control the pathogen are needed. One of these options is the use of specific egg yolk antibodies (IgY) for passive immunotherapy. Thus, the aim of our work was to produce IgY antibodies against an equine S. Newport strain and assess their in vitro inhibitory activity. To this end, laying hens were immunized with an inactivated S. Newport strain by using either Freund's or Montanide adjuvant and egg yolk extracts were obtained. The levels of specific IgY antibodies against Salmonella in sera and egg extracts were determined by dot-blot and microagglutination. Besides, the IgY extracts were characterized by total protein analysis, SDS-PAGE, Western Blot, and inhibition of bacterial motility. IgY extracts showed high purity (87.7 to 91.8 %), high microagglutination titers, and the ability to inhibit the motility of the bacterium. The results using Montanide were similar to those using the traditional Freund's adjuvant. Thus, Montanide may also be a good adjuvant to produce IgY. IgY-technology represents a potential tool for the control of salmonellosis in horses.
Instituto de Patobiología
Fil: Bustos, Carla Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Bustos, Carla Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bustos, Carla Paola. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Enfermedades Infecciosas; Argentina
Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gambarotta, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Bioestadística; Argentina
Fil: Guida, Nora. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Enfermedades Infecciosas; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fuente
Journal of Equine Veterinary Science 103 : 103657 (Agosto 2021)
Materia
Salmonella
Caballos
Anticuerpos
Yema de Huevo
Horses
Antibodies
Egg Yolk
In Vitro
Salmonella newport
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/12744
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oai_identifier_str oai:localhost:20.500.12123/12744
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spelling In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horsesBustos, Carla PaolaLeiva, Carlos LeónidasGambarotta, MarianaGuida, NoraChacana, PabloSalmonellaCaballosAnticuerposYema de HuevoHorsesAntibodiesEgg YolkIn VitroSalmonella newportEquine salmonellosis is caused by several Salmonella serotypes, including Salmonella Newport, which cause enterocolitis and diarrhea. Treatment usually includes the administration of antibiotics. However, since multidrug-resistant Salmonella is commonly detected, alternative options to control the pathogen are needed. One of these options is the use of specific egg yolk antibodies (IgY) for passive immunotherapy. Thus, the aim of our work was to produce IgY antibodies against an equine S. Newport strain and assess their in vitro inhibitory activity. To this end, laying hens were immunized with an inactivated S. Newport strain by using either Freund's or Montanide adjuvant and egg yolk extracts were obtained. The levels of specific IgY antibodies against Salmonella in sera and egg extracts were determined by dot-blot and microagglutination. Besides, the IgY extracts were characterized by total protein analysis, SDS-PAGE, Western Blot, and inhibition of bacterial motility. IgY extracts showed high purity (87.7 to 91.8 %), high microagglutination titers, and the ability to inhibit the motility of the bacterium. The results using Montanide were similar to those using the traditional Freund's adjuvant. Thus, Montanide may also be a good adjuvant to produce IgY. IgY-technology represents a potential tool for the control of salmonellosis in horses.Instituto de PatobiologíaFil: Bustos, Carla Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Bustos, Carla Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Bustos, Carla Paola. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Enfermedades Infecciosas; ArgentinaFil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Gambarotta, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Bioestadística; ArgentinaFil: Guida, Nora. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Enfermedades Infecciosas; ArgentinaFil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; ArgentinaFil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaElsevier2022-08-31T16:19:05Z2022-08-31T16:19:05Z2021-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/12744https://www.sciencedirect.com/science/article/abs/pii/S07370806210028720737-0806https://doi.org/10.1016/j.jevs.2021.103657Journal of Equine Veterinary Science 103 : 103657 (Agosto 2021)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repograntAgreement/INTA/2019-PpAT-2.3.1-S.ANIM-001/2019-PpAT-2.3.1-S.ANIM-001/AR./Programa por Área Temática Salud Animalinfo:eu-repo/semantics/restrictedAccess2025-10-16T09:30:53Zoai:localhost:20.500.12123/12744instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-10-16 09:30:54.283INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses
title In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses
spellingShingle In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses
Bustos, Carla Paola
Salmonella
Caballos
Anticuerpos
Yema de Huevo
Horses
Antibodies
Egg Yolk
In Vitro
Salmonella newport
title_short In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses
title_full In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses
title_fullStr In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses
title_full_unstemmed In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses
title_sort In vitro inhibitory activity of IgY antibodies against Salmonella ser. newport isolated from horses
dc.creator.none.fl_str_mv Bustos, Carla Paola
Leiva, Carlos Leónidas
Gambarotta, Mariana
Guida, Nora
Chacana, Pablo
author Bustos, Carla Paola
author_facet Bustos, Carla Paola
Leiva, Carlos Leónidas
Gambarotta, Mariana
Guida, Nora
Chacana, Pablo
author_role author
author2 Leiva, Carlos Leónidas
Gambarotta, Mariana
Guida, Nora
Chacana, Pablo
author2_role author
author
author
author
dc.subject.none.fl_str_mv Salmonella
Caballos
Anticuerpos
Yema de Huevo
Horses
Antibodies
Egg Yolk
In Vitro
Salmonella newport
topic Salmonella
Caballos
Anticuerpos
Yema de Huevo
Horses
Antibodies
Egg Yolk
In Vitro
Salmonella newport
dc.description.none.fl_txt_mv Equine salmonellosis is caused by several Salmonella serotypes, including Salmonella Newport, which cause enterocolitis and diarrhea. Treatment usually includes the administration of antibiotics. However, since multidrug-resistant Salmonella is commonly detected, alternative options to control the pathogen are needed. One of these options is the use of specific egg yolk antibodies (IgY) for passive immunotherapy. Thus, the aim of our work was to produce IgY antibodies against an equine S. Newport strain and assess their in vitro inhibitory activity. To this end, laying hens were immunized with an inactivated S. Newport strain by using either Freund's or Montanide adjuvant and egg yolk extracts were obtained. The levels of specific IgY antibodies against Salmonella in sera and egg extracts were determined by dot-blot and microagglutination. Besides, the IgY extracts were characterized by total protein analysis, SDS-PAGE, Western Blot, and inhibition of bacterial motility. IgY extracts showed high purity (87.7 to 91.8 %), high microagglutination titers, and the ability to inhibit the motility of the bacterium. The results using Montanide were similar to those using the traditional Freund's adjuvant. Thus, Montanide may also be a good adjuvant to produce IgY. IgY-technology represents a potential tool for the control of salmonellosis in horses.
Instituto de Patobiología
Fil: Bustos, Carla Paola. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Bustos, Carla Paola. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Bustos, Carla Paola. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Enfermedades Infecciosas; Argentina
Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Gambarotta, Mariana. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Bioestadística; Argentina
Fil: Guida, Nora. Universidad de Buenos Aires. Facultad de Ciencias Veterinarias. Cátedra de Enfermedades Infecciosas; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología; Argentina
Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
description Equine salmonellosis is caused by several Salmonella serotypes, including Salmonella Newport, which cause enterocolitis and diarrhea. Treatment usually includes the administration of antibiotics. However, since multidrug-resistant Salmonella is commonly detected, alternative options to control the pathogen are needed. One of these options is the use of specific egg yolk antibodies (IgY) for passive immunotherapy. Thus, the aim of our work was to produce IgY antibodies against an equine S. Newport strain and assess their in vitro inhibitory activity. To this end, laying hens were immunized with an inactivated S. Newport strain by using either Freund's or Montanide adjuvant and egg yolk extracts were obtained. The levels of specific IgY antibodies against Salmonella in sera and egg extracts were determined by dot-blot and microagglutination. Besides, the IgY extracts were characterized by total protein analysis, SDS-PAGE, Western Blot, and inhibition of bacterial motility. IgY extracts showed high purity (87.7 to 91.8 %), high microagglutination titers, and the ability to inhibit the motility of the bacterium. The results using Montanide were similar to those using the traditional Freund's adjuvant. Thus, Montanide may also be a good adjuvant to produce IgY. IgY-technology represents a potential tool for the control of salmonellosis in horses.
publishDate 2021
dc.date.none.fl_str_mv 2021-08
2022-08-31T16:19:05Z
2022-08-31T16:19:05Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/12744
https://www.sciencedirect.com/science/article/abs/pii/S0737080621002872
0737-0806
https://doi.org/10.1016/j.jevs.2021.103657
url http://hdl.handle.net/20.500.12123/12744
https://www.sciencedirect.com/science/article/abs/pii/S0737080621002872
https://doi.org/10.1016/j.jevs.2021.103657
identifier_str_mv 0737-0806
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repograntAgreement/INTA/2019-PpAT-2.3.1-S.ANIM-001/2019-PpAT-2.3.1-S.ANIM-001/AR./Programa por Área Temática Salud Animal
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of Equine Veterinary Science 103 : 103657 (Agosto 2021)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.712165

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