Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies

Autores
Bellido, Demian; Chacana, Pablo; Mozgovoj, Marina Valeria; Gonzalez, Diego; Goldbaum, Fernando Alberto; Wigdorovitz, Andres; Dus Santos, Maria Jose
Año de publicación
2012
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Lumazine synthase from Brucella spp. (BLS) is a highly immunogenic decameric protein. It has been previously described as a carrier of peptides or proteins to increase their immunogenicity in different animal species, but its activity has never been evaluated in chickens. In this work, the use of BLS to improve the antibody response against bovine rotavirus (BRV) VP8d protein in laying hens was assessed. VP8d is the inner domain of the VP8 spike protein which preserves the sialic acid binding activity and the neutralizing epitopes present in the viral protein. Hens were immunized three times with 2 μg of VP8d alone or fused to BLS. Hens inoculated with BLSVP8d developed higher antibody titers (evaluated by ELISA and viral neutralization test) than hens immunized either with VP8d alone or the mixture of VP8d and BLS. Furthermore, IgY antibodies against BLSVP8d were able to fully protect mice against challenge with virulent BRV in a dose-depent-manner. Overall, these results demonstrate that BLS is a potent immonumodulator that enhances the antibody response in hens, thus increasing the concentration of specific IgY in the egg yolk, one of the main issues to be adressed in order to improve the use of the IgY technology.
Instituto de Virología
Fil: Bellido, Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Gonzalez, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Goldbaum, Fernando Alberto. Fundación Instituto Leloir; Argentina
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fuente
Advances in Bioscience and Biotechnology 3 (1) : 80-86 (February 2012)
Materia
Brucella
Inmunogenética
Proteínas
Yema de Huevo
Rotavirus Bovino
Anticuerpos
Immunogenetics
Proteins
Egg Yolk
Bovine Rotavirus
Antibodies
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/4341
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oai_identifier_str oai:localhost:20.500.12123/4341
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodiesBellido, DemianChacana, PabloMozgovoj, Marina ValeriaGonzalez, DiegoGoldbaum, Fernando AlbertoWigdorovitz, AndresDus Santos, Maria JoseBrucellaInmunogenéticaProteínasYema de HuevoRotavirus BovinoAnticuerposImmunogeneticsProteinsEgg YolkBovine RotavirusAntibodiesLumazine synthase from Brucella spp. (BLS) is a highly immunogenic decameric protein. It has been previously described as a carrier of peptides or proteins to increase their immunogenicity in different animal species, but its activity has never been evaluated in chickens. In this work, the use of BLS to improve the antibody response against bovine rotavirus (BRV) VP8d protein in laying hens was assessed. VP8d is the inner domain of the VP8 spike protein which preserves the sialic acid binding activity and the neutralizing epitopes present in the viral protein. Hens were immunized three times with 2 μg of VP8d alone or fused to BLS. Hens inoculated with BLSVP8d developed higher antibody titers (evaluated by ELISA and viral neutralization test) than hens immunized either with VP8d alone or the mixture of VP8d and BLS. Furthermore, IgY antibodies against BLSVP8d were able to fully protect mice against challenge with virulent BRV in a dose-depent-manner. Overall, these results demonstrate that BLS is a potent immonumodulator that enhances the antibody response in hens, thus increasing the concentration of specific IgY in the egg yolk, one of the main issues to be adressed in order to improve the use of the IgY technology.Instituto de VirologíaFil: Bellido, Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Gonzalez, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Goldbaum, Fernando Alberto. Fundación Instituto Leloir; ArgentinaFil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaScientific Research2019-01-28T15:48:56Z2019-01-28T15:48:56Z2012-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttps://www.scirp.org/journal/PaperInformation.aspx?PaperID=17200http://hdl.handle.net/20.500.12123/43412156-84562156-8502http://dx.doi.org/10.4236/abb.2012.31012Advances in Bioscience and Biotechnology 3 (1) : 80-86 (February 2012)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:44:33Zoai:localhost:20.500.12123/4341instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:34.166INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies
title Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies
spellingShingle Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies
Bellido, Demian
Brucella
Inmunogenética
Proteínas
Yema de Huevo
Rotavirus Bovino
Anticuerpos
Immunogenetics
Proteins
Egg Yolk
Bovine Rotavirus
Antibodies
title_short Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies
title_full Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies
title_fullStr Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies
title_full_unstemmed Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies
title_sort Brucella spp. Lumazine synthase as a novel immunomodulator to produce egg yolk antibodies
dc.creator.none.fl_str_mv Bellido, Demian
Chacana, Pablo
Mozgovoj, Marina Valeria
Gonzalez, Diego
Goldbaum, Fernando Alberto
Wigdorovitz, Andres
Dus Santos, Maria Jose
author Bellido, Demian
author_facet Bellido, Demian
Chacana, Pablo
Mozgovoj, Marina Valeria
Gonzalez, Diego
Goldbaum, Fernando Alberto
Wigdorovitz, Andres
Dus Santos, Maria Jose
author_role author
author2 Chacana, Pablo
Mozgovoj, Marina Valeria
Gonzalez, Diego
Goldbaum, Fernando Alberto
Wigdorovitz, Andres
Dus Santos, Maria Jose
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Brucella
Inmunogenética
Proteínas
Yema de Huevo
Rotavirus Bovino
Anticuerpos
Immunogenetics
Proteins
Egg Yolk
Bovine Rotavirus
Antibodies
topic Brucella
Inmunogenética
Proteínas
Yema de Huevo
Rotavirus Bovino
Anticuerpos
Immunogenetics
Proteins
Egg Yolk
Bovine Rotavirus
Antibodies
dc.description.none.fl_txt_mv Lumazine synthase from Brucella spp. (BLS) is a highly immunogenic decameric protein. It has been previously described as a carrier of peptides or proteins to increase their immunogenicity in different animal species, but its activity has never been evaluated in chickens. In this work, the use of BLS to improve the antibody response against bovine rotavirus (BRV) VP8d protein in laying hens was assessed. VP8d is the inner domain of the VP8 spike protein which preserves the sialic acid binding activity and the neutralizing epitopes present in the viral protein. Hens were immunized three times with 2 μg of VP8d alone or fused to BLS. Hens inoculated with BLSVP8d developed higher antibody titers (evaluated by ELISA and viral neutralization test) than hens immunized either with VP8d alone or the mixture of VP8d and BLS. Furthermore, IgY antibodies against BLSVP8d were able to fully protect mice against challenge with virulent BRV in a dose-depent-manner. Overall, these results demonstrate that BLS is a potent immonumodulator that enhances the antibody response in hens, thus increasing the concentration of specific IgY in the egg yolk, one of the main issues to be adressed in order to improve the use of the IgY technology.
Instituto de Virología
Fil: Bellido, Demian. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Mozgovoj, Marina Valeria. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Gonzalez, Diego. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Goldbaum, Fernando Alberto. Fundación Instituto Leloir; Argentina
Fil: Wigdorovitz, Andres. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Dus Santos, Maria Jose. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
description Lumazine synthase from Brucella spp. (BLS) is a highly immunogenic decameric protein. It has been previously described as a carrier of peptides or proteins to increase their immunogenicity in different animal species, but its activity has never been evaluated in chickens. In this work, the use of BLS to improve the antibody response against bovine rotavirus (BRV) VP8d protein in laying hens was assessed. VP8d is the inner domain of the VP8 spike protein which preserves the sialic acid binding activity and the neutralizing epitopes present in the viral protein. Hens were immunized three times with 2 μg of VP8d alone or fused to BLS. Hens inoculated with BLSVP8d developed higher antibody titers (evaluated by ELISA and viral neutralization test) than hens immunized either with VP8d alone or the mixture of VP8d and BLS. Furthermore, IgY antibodies against BLSVP8d were able to fully protect mice against challenge with virulent BRV in a dose-depent-manner. Overall, these results demonstrate that BLS is a potent immonumodulator that enhances the antibody response in hens, thus increasing the concentration of specific IgY in the egg yolk, one of the main issues to be adressed in order to improve the use of the IgY technology.
publishDate 2012
dc.date.none.fl_str_mv 2012-02
2019-01-28T15:48:56Z
2019-01-28T15:48:56Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://www.scirp.org/journal/PaperInformation.aspx?PaperID=17200
http://hdl.handle.net/20.500.12123/4341
2156-8456
2156-8502
http://dx.doi.org/10.4236/abb.2012.31012
url https://www.scirp.org/journal/PaperInformation.aspx?PaperID=17200
http://hdl.handle.net/20.500.12123/4341
http://dx.doi.org/10.4236/abb.2012.31012
identifier_str_mv 2156-8456
2156-8502
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Scientific Research
publisher.none.fl_str_mv Scientific Research
dc.source.none.fl_str_mv Advances in Bioscience and Biotechnology 3 (1) : 80-86 (February 2012)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.558318

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