A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction

Autores
González Viacava, Myriam Belén; Leiva, Carlos Leónidas; Tavarone, Maria Eugenia; Dokmetjian, José Christian; Cascone, Osvaldo; Chacana, Pablo; Fingermann, Matías
Año de publicación
2022
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Although mammals are still the main source of polyclonal antibodies production, the use of egg yolk immunoglobulins (IgY) is increasing, and thus improvement of its safety to reduce undesired reactions is needed. Removal of IgY constant domains by pepsin enzymatic treatment is expected to reduce potential adverse effects derived from the parenteral administration of these antibodies, while also increasing its distribution volume. Current Fab’ production requires the previous purification of IgY to be used as starting material. In this context, relevant economic benefits may be gained if initial IgY purification could be avoided, by using rawer starting materials. In this work, a three-step process for Fab’ production from crude egg yolk water-soluble fractions is described using scalable and simple low-cost technologies such as ultrafiltration and anion exchange chromatography. The overall process yield of 33% highly pure Fab’ from water-soluble fractions favorably compares to the manufacture of related medicinal products from mammalian antibodies, such as antivenoms.
Instituto de Patobiología
Fil: González Viacava, Myriam Belén. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; Argentina
Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tavarone, Maria Eugenia. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; Argentina
Fil: Dokmetjian, José Christian. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; Argentina
Fil: Cascone, Osvaldo. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; Argentina
Fil: Cascone, Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Cascone, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fingermann, Matías. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; Argentina
Fuente
Journal of Chromatography B 1211 : 123486 (Noviembre 2022)
Materia
Egg Yolk
Antibodies
Purification
Chromatography
Yema de Huevo
Anticuerpos
Purificación
Cromatografía
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/13964
Acceder
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oai_identifier_str oai:localhost:20.500.12123/13964
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fractionGonzález Viacava, Myriam BelénLeiva, Carlos LeónidasTavarone, Maria EugeniaDokmetjian, José ChristianCascone, OsvaldoChacana, PabloFingermann, MatíasEgg YolkAntibodiesPurificationChromatographyYema de HuevoAnticuerposPurificaciónCromatografíaAlthough mammals are still the main source of polyclonal antibodies production, the use of egg yolk immunoglobulins (IgY) is increasing, and thus improvement of its safety to reduce undesired reactions is needed. Removal of IgY constant domains by pepsin enzymatic treatment is expected to reduce potential adverse effects derived from the parenteral administration of these antibodies, while also increasing its distribution volume. Current Fab’ production requires the previous purification of IgY to be used as starting material. In this context, relevant economic benefits may be gained if initial IgY purification could be avoided, by using rawer starting materials. In this work, a three-step process for Fab’ production from crude egg yolk water-soluble fractions is described using scalable and simple low-cost technologies such as ultrafiltration and anion exchange chromatography. The overall process yield of 33% highly pure Fab’ from water-soluble fractions favorably compares to the manufacture of related medicinal products from mammalian antibodies, such as antivenoms.Instituto de PatobiologíaFil: González Viacava, Myriam Belén. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; ArgentinaFil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Tavarone, Maria Eugenia. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; ArgentinaFil: Dokmetjian, José Christian. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; ArgentinaFil: Cascone, Osvaldo. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; ArgentinaFil: Cascone, Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; ArgentinaFil: Cascone, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; ArgentinaFil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Fingermann, Matías. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; ArgentinaElsevier2023-02-14T11:52:24Z2023-02-14T11:52:24Z2022-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/13964https://www.sciencedirect.com/science/article/pii/S15700232220039071873-376Xhttps://doi.org/10.1016/j.jchromb.2022.123486Journal of Chromatography B 1211 : 123486 (Noviembre 2022)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:45:53Zoai:localhost:20.500.12123/13964instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:54.207INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction
title A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction
spellingShingle A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction
González Viacava, Myriam Belén
Egg Yolk
Antibodies
Purification
Chromatography
Yema de Huevo
Anticuerpos
Purificación
Cromatografía
title_short A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction
title_full A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction
title_fullStr A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction
title_full_unstemmed A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction
title_sort A simple and economic three-step process for producing highly purified Fab’ fragments directly from the egg yolk water-soluble fraction
dc.creator.none.fl_str_mv González Viacava, Myriam Belén
Leiva, Carlos Leónidas
Tavarone, Maria Eugenia
Dokmetjian, José Christian
Cascone, Osvaldo
Chacana, Pablo
Fingermann, Matías
author González Viacava, Myriam Belén
author_facet González Viacava, Myriam Belén
Leiva, Carlos Leónidas
Tavarone, Maria Eugenia
Dokmetjian, José Christian
Cascone, Osvaldo
Chacana, Pablo
Fingermann, Matías
author_role author
author2 Leiva, Carlos Leónidas
Tavarone, Maria Eugenia
Dokmetjian, José Christian
Cascone, Osvaldo
Chacana, Pablo
Fingermann, Matías
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Egg Yolk
Antibodies
Purification
Chromatography
Yema de Huevo
Anticuerpos
Purificación
Cromatografía
topic Egg Yolk
Antibodies
Purification
Chromatography
Yema de Huevo
Anticuerpos
Purificación
Cromatografía
dc.description.none.fl_txt_mv Although mammals are still the main source of polyclonal antibodies production, the use of egg yolk immunoglobulins (IgY) is increasing, and thus improvement of its safety to reduce undesired reactions is needed. Removal of IgY constant domains by pepsin enzymatic treatment is expected to reduce potential adverse effects derived from the parenteral administration of these antibodies, while also increasing its distribution volume. Current Fab’ production requires the previous purification of IgY to be used as starting material. In this context, relevant economic benefits may be gained if initial IgY purification could be avoided, by using rawer starting materials. In this work, a three-step process for Fab’ production from crude egg yolk water-soluble fractions is described using scalable and simple low-cost technologies such as ultrafiltration and anion exchange chromatography. The overall process yield of 33% highly pure Fab’ from water-soluble fractions favorably compares to the manufacture of related medicinal products from mammalian antibodies, such as antivenoms.
Instituto de Patobiología
Fil: González Viacava, Myriam Belén. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; Argentina
Fil: Leiva, Carlos Leónidas. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Leiva, Carlos Leónidas. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Tavarone, Maria Eugenia. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; Argentina
Fil: Dokmetjian, José Christian. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; Argentina
Fil: Cascone, Osvaldo. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; Argentina
Fil: Cascone, Osvaldo. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica. Instituto de Nanobiotecnología; Argentina
Fil: Cascone, Osvaldo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Chacana, Pablo. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Patobiología Veterinaria; Argentina
Fil: Chacana, Pablo. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Fingermann, Matías. Administración Nacional de Laboratorios e Institutos de Salud Dr. Carlos Malbrán (ANLIS). Instituto Nacional de Producción de Biológicos; Argentina
description Although mammals are still the main source of polyclonal antibodies production, the use of egg yolk immunoglobulins (IgY) is increasing, and thus improvement of its safety to reduce undesired reactions is needed. Removal of IgY constant domains by pepsin enzymatic treatment is expected to reduce potential adverse effects derived from the parenteral administration of these antibodies, while also increasing its distribution volume. Current Fab’ production requires the previous purification of IgY to be used as starting material. In this context, relevant economic benefits may be gained if initial IgY purification could be avoided, by using rawer starting materials. In this work, a three-step process for Fab’ production from crude egg yolk water-soluble fractions is described using scalable and simple low-cost technologies such as ultrafiltration and anion exchange chromatography. The overall process yield of 33% highly pure Fab’ from water-soluble fractions favorably compares to the manufacture of related medicinal products from mammalian antibodies, such as antivenoms.
publishDate 2022
dc.date.none.fl_str_mv 2022-11
2023-02-14T11:52:24Z
2023-02-14T11:52:24Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/13964
https://www.sciencedirect.com/science/article/pii/S1570023222003907
1873-376X
https://doi.org/10.1016/j.jchromb.2022.123486
url http://hdl.handle.net/20.500.12123/13964
https://www.sciencedirect.com/science/article/pii/S1570023222003907
https://doi.org/10.1016/j.jchromb.2022.123486
identifier_str_mv 1873-376X
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Journal of Chromatography B 1211 : 123486 (Noviembre 2022)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.558318

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