Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress

Autores
Causin, Humberto Fabio; Marchetti, Cintia Florencia; Pena, Liliana Beatriz; Gallego, Susana Mabel; Barneix, Atilio Jose
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In shaded wheat (Triticum aestivum L.) leaves, the suppression of blue radiation (BR) triggers senescence. This phenomenon is correlated to an increase in oxidative stress symptoms and a decrease of catalase (CAT) activity, among other traits. Previous data suggest that the radiation signal transduction pathway may involve changes in Ca2+ and H2O2 homeostasis. For better a understanding of the interaction among the spectral composition of radiation, Ca2+ availability, and the antioxidant metabolism in the regulation of shade-induced senescence, detached wheat leaves were placed in a growth chamber and exposed to either blue (B, high BR transmittance) and/or green (G, very low BR transmittance) Lee® filters in the absence or presence of 0.8 mM verapamil (a Ca2+ channels blocker), 4.0 mM EGTA (a Ca2+chelator), or 8.0 mM 3-amino-1,2,4-triazole (a CAT inhibitor). At defined time points, the leaf samples were analyzed for changes in chlorophyll content, specific activities of CAT, ascorbate peroxidase (APX), and guaiacol peroxidase (POX), CAT isozymes, and gene expression of CAT1, CAT2, and two senescence markers (TaSAG1 and TaSAG3). BR transmittance decreased the chlorophyll degradation rate and SAG genes expression either in leaves continuously exposed under the B filter, as well as in leaves previously exposed under the G filter. The effect of BR was associated with the maintenance of a high CAT (but not APX and POX) activity, and it was suppressed either in the presence of 3-AT or when Ca2+ availability was decreased. BR altered the CAT activity both at the transcriptional and at the posttranscriptional level. Nevertheless, different responses of CAT isozymes and CAT genes expression profiles to specific treatment combinations indicate that they differed in their regulatory pathways.
Instituto de Suelos
Fil: Causin, Humberto Fabio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Marchetti, Cintia Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Pena, Liliana Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Gallego, Susana Mabel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Barneix, Atilio José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina
Fuente
Biologia plantarum 59 (1) : 154–162. (January 2015)
Materia
Trigo
Triticum Aestivum
Catalasa
Antioxidantes
Calcio
Radiación
Estrés Oxidativo
Expresión Génica
Wheat
Catalase
Antioxidants
Calcium
Radiation
Oxidative Stress
Gene Expression
Blue Radiation
Senescencia
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/3558
Acceder
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oai_identifier_str oai:localhost:20.500.12123/3558
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network_name_str INTA Digital (INTA)
spelling Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stressCausin, Humberto FabioMarchetti, Cintia FlorenciaPena, Liliana BeatrizGallego, Susana MabelBarneix, Atilio JoseTrigoTriticum AestivumCatalasaAntioxidantesCalcioRadiaciónEstrés OxidativoExpresión GénicaWheatCatalaseAntioxidantsCalciumRadiationOxidative StressGene ExpressionBlue RadiationSenescenciaIn shaded wheat (Triticum aestivum L.) leaves, the suppression of blue radiation (BR) triggers senescence. This phenomenon is correlated to an increase in oxidative stress symptoms and a decrease of catalase (CAT) activity, among other traits. Previous data suggest that the radiation signal transduction pathway may involve changes in Ca2+ and H2O2 homeostasis. For better a understanding of the interaction among the spectral composition of radiation, Ca2+ availability, and the antioxidant metabolism in the regulation of shade-induced senescence, detached wheat leaves were placed in a growth chamber and exposed to either blue (B, high BR transmittance) and/or green (G, very low BR transmittance) Lee® filters in the absence or presence of 0.8 mM verapamil (a Ca2+ channels blocker), 4.0 mM EGTA (a Ca2+chelator), or 8.0 mM 3-amino-1,2,4-triazole (a CAT inhibitor). At defined time points, the leaf samples were analyzed for changes in chlorophyll content, specific activities of CAT, ascorbate peroxidase (APX), and guaiacol peroxidase (POX), CAT isozymes, and gene expression of CAT1, CAT2, and two senescence markers (TaSAG1 and TaSAG3). BR transmittance decreased the chlorophyll degradation rate and SAG genes expression either in leaves continuously exposed under the B filter, as well as in leaves previously exposed under the G filter. The effect of BR was associated with the maintenance of a high CAT (but not APX and POX) activity, and it was suppressed either in the presence of 3-AT or when Ca2+ availability was decreased. BR altered the CAT activity both at the transcriptional and at the posttranscriptional level. Nevertheless, different responses of CAT isozymes and CAT genes expression profiles to specific treatment combinations indicate that they differed in their regulatory pathways.Instituto de SuelosFil: Causin, Humberto Fabio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Marchetti, Cintia Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Pena, Liliana Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Gallego, Susana Mabel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Barneix, Atilio José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; ArgentinaSpringer2018-10-04T16:13:50Z2018-10-04T16:13:50Z2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/3558https://link.springer.com/article/10.1007/s10535-014-0480-z0006-31341573-8264 (Online)https://doi.org/10.1007/s10535-014-0480-zBiologia plantarum 59 (1) : 154–162. (January 2015)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/restrictedAccess2025-09-29T13:44:27Zoai:localhost:20.500.12123/3558instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:27.713INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
title Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
spellingShingle Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
Causin, Humberto Fabio
Trigo
Triticum Aestivum
Catalasa
Antioxidantes
Calcio
Radiación
Estrés Oxidativo
Expresión Génica
Wheat
Catalase
Antioxidants
Calcium
Radiation
Oxidative Stress
Gene Expression
Blue Radiation
Senescencia
title_short Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
title_full Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
title_fullStr Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
title_full_unstemmed Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
title_sort Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
dc.creator.none.fl_str_mv Causin, Humberto Fabio
Marchetti, Cintia Florencia
Pena, Liliana Beatriz
Gallego, Susana Mabel
Barneix, Atilio Jose
author Causin, Humberto Fabio
author_facet Causin, Humberto Fabio
Marchetti, Cintia Florencia
Pena, Liliana Beatriz
Gallego, Susana Mabel
Barneix, Atilio Jose
author_role author
author2 Marchetti, Cintia Florencia
Pena, Liliana Beatriz
Gallego, Susana Mabel
Barneix, Atilio Jose
author2_role author
author
author
author
dc.subject.none.fl_str_mv Trigo
Triticum Aestivum
Catalasa
Antioxidantes
Calcio
Radiación
Estrés Oxidativo
Expresión Génica
Wheat
Catalase
Antioxidants
Calcium
Radiation
Oxidative Stress
Gene Expression
Blue Radiation
Senescencia
topic Trigo
Triticum Aestivum
Catalasa
Antioxidantes
Calcio
Radiación
Estrés Oxidativo
Expresión Génica
Wheat
Catalase
Antioxidants
Calcium
Radiation
Oxidative Stress
Gene Expression
Blue Radiation
Senescencia
dc.description.none.fl_txt_mv In shaded wheat (Triticum aestivum L.) leaves, the suppression of blue radiation (BR) triggers senescence. This phenomenon is correlated to an increase in oxidative stress symptoms and a decrease of catalase (CAT) activity, among other traits. Previous data suggest that the radiation signal transduction pathway may involve changes in Ca2+ and H2O2 homeostasis. For better a understanding of the interaction among the spectral composition of radiation, Ca2+ availability, and the antioxidant metabolism in the regulation of shade-induced senescence, detached wheat leaves were placed in a growth chamber and exposed to either blue (B, high BR transmittance) and/or green (G, very low BR transmittance) Lee® filters in the absence or presence of 0.8 mM verapamil (a Ca2+ channels blocker), 4.0 mM EGTA (a Ca2+chelator), or 8.0 mM 3-amino-1,2,4-triazole (a CAT inhibitor). At defined time points, the leaf samples were analyzed for changes in chlorophyll content, specific activities of CAT, ascorbate peroxidase (APX), and guaiacol peroxidase (POX), CAT isozymes, and gene expression of CAT1, CAT2, and two senescence markers (TaSAG1 and TaSAG3). BR transmittance decreased the chlorophyll degradation rate and SAG genes expression either in leaves continuously exposed under the B filter, as well as in leaves previously exposed under the G filter. The effect of BR was associated with the maintenance of a high CAT (but not APX and POX) activity, and it was suppressed either in the presence of 3-AT or when Ca2+ availability was decreased. BR altered the CAT activity both at the transcriptional and at the posttranscriptional level. Nevertheless, different responses of CAT isozymes and CAT genes expression profiles to specific treatment combinations indicate that they differed in their regulatory pathways.
Instituto de Suelos
Fil: Causin, Humberto Fabio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Marchetti, Cintia Florencia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Pena, Liliana Beatriz. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Gallego, Susana Mabel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Barneix, Atilio José. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Suelos; Argentina
description In shaded wheat (Triticum aestivum L.) leaves, the suppression of blue radiation (BR) triggers senescence. This phenomenon is correlated to an increase in oxidative stress symptoms and a decrease of catalase (CAT) activity, among other traits. Previous data suggest that the radiation signal transduction pathway may involve changes in Ca2+ and H2O2 homeostasis. For better a understanding of the interaction among the spectral composition of radiation, Ca2+ availability, and the antioxidant metabolism in the regulation of shade-induced senescence, detached wheat leaves were placed in a growth chamber and exposed to either blue (B, high BR transmittance) and/or green (G, very low BR transmittance) Lee® filters in the absence or presence of 0.8 mM verapamil (a Ca2+ channels blocker), 4.0 mM EGTA (a Ca2+chelator), or 8.0 mM 3-amino-1,2,4-triazole (a CAT inhibitor). At defined time points, the leaf samples were analyzed for changes in chlorophyll content, specific activities of CAT, ascorbate peroxidase (APX), and guaiacol peroxidase (POX), CAT isozymes, and gene expression of CAT1, CAT2, and two senescence markers (TaSAG1 and TaSAG3). BR transmittance decreased the chlorophyll degradation rate and SAG genes expression either in leaves continuously exposed under the B filter, as well as in leaves previously exposed under the G filter. The effect of BR was associated with the maintenance of a high CAT (but not APX and POX) activity, and it was suppressed either in the presence of 3-AT or when Ca2+ availability was decreased. BR altered the CAT activity both at the transcriptional and at the posttranscriptional level. Nevertheless, different responses of CAT isozymes and CAT genes expression profiles to specific treatment combinations indicate that they differed in their regulatory pathways.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
2018-10-04T16:13:50Z
2018-10-04T16:13:50Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/3558
https://link.springer.com/article/10.1007/s10535-014-0480-z
0006-3134
1573-8264 (Online)
https://doi.org/10.1007/s10535-014-0480-z
url http://hdl.handle.net/20.500.12123/3558
https://link.springer.com/article/10.1007/s10535-014-0480-z
https://doi.org/10.1007/s10535-014-0480-z
identifier_str_mv 0006-3134
1573-8264 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv Biologia plantarum 59 (1) : 154–162. (January 2015)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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score 12.559606

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