Growth, toxin production, active oxygen species and catalase activity of Microcystis aeruginosa (Cyanophyceae) exposed to temperature stress

Autores
Giannuzzi, Leda; Krock, Bernd; Crettaz Minaglia, Melina Celeste; Rosso, Lorena Vanesa; Houghton, Christian; Sedan, Daniela Yazmine; Malanga, Gabriela Fabiana; Espinosa, Mariela; Andrinolo, Dario; Hernando, Marcelo Pablo
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Microcystis are known for their potential ability to synthesize toxins, mainly microcystins (MCs). In order to evaluate the effects of temperature on chlorophyll a (Chl a), growth, physiological responses and toxin production of a native Microcystis aeruginosa, we exposed the cells to low (23 °C) and high (29 °C) temperature in addition to a 26 °C control treatment. Exponential growth rate was significantly higher at 29 °C compared to 23 °C and control, reaching 0.43, 0.32 and 0.33 day−1 respectively. In addition, there was a delay of the start of exponential growth at 23 °C. However, the intracellular concentration of Chl a decreased significantly due to temperature change. A significant increase in intracellular ROS was observed in coincidence with the activation of enzymatic antioxidant catalase (CAT) during the first two days of exposure to 23° and 29 °C in comparison to the control experiment, decreasing thereafter to nearly initial values. Five MCs were determined by LC-MS/MS analysis. In the experiments, the highest MC concentration, 205 fg [Leu1] MC-LR.cell− 1 expressed as MC-LR equivalent was measured in the beginning of the experiment and subsequently declined to 160 fg.cell− 1 on day 2 and 70 fg.cell− 1 on day 4 in cells exposed to 29 °C. The same trend was observed for all other MCs except for the least abundant MC-LR which showed a continuous increase during exposure time. Our results suggest a high ability of M. aeruginosa to perceive ROS and to rapidly initiate antioxidant defenses with a differential response on MC production.
Fil: Giannuzzi, Leda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; Argentina
Fil: Krock, Bernd. Alfred Wegener Institute; Alemania
Fil: Crettaz Minaglia, Melina Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; Argentina
Fil: Rosso, Lorena Vanesa. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; Argentina
Fil: Houghton, Christian. Comisión Nacional de Energía Atómica; Argentina
Fil: Sedan, Daniela Yazmine. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; Argentina
Fil: Malanga, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Espinosa, Mariela. Comisión Nacional de Energía Atómica; Argentina
Fil: Andrinolo, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; Argentina
Fil: Hernando, Marcelo Pablo. Comisión Nacional de Energía Atómica; Argentina
Materia
Catalase
Chlorophyll
Microcystin Variants
Ros
Catalase
Chlorophyll
Ros
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/47439
Acceder
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network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Growth, toxin production, active oxygen species and catalase activity of Microcystis aeruginosa (Cyanophyceae) exposed to temperature stressGiannuzzi, LedaKrock, BerndCrettaz Minaglia, Melina CelesteRosso, Lorena VanesaHoughton, ChristianSedan, Daniela YazmineMalanga, Gabriela FabianaEspinosa, MarielaAndrinolo, DarioHernando, Marcelo PabloCatalaseChlorophyllMicrocystin VariantsRosCatalaseChlorophyllRoshttps://purl.org/becyt/ford/3.1https://purl.org/becyt/ford/3Microcystis are known for their potential ability to synthesize toxins, mainly microcystins (MCs). In order to evaluate the effects of temperature on chlorophyll a (Chl a), growth, physiological responses and toxin production of a native Microcystis aeruginosa, we exposed the cells to low (23 °C) and high (29 °C) temperature in addition to a 26 °C control treatment. Exponential growth rate was significantly higher at 29 °C compared to 23 °C and control, reaching 0.43, 0.32 and 0.33 day−1 respectively. In addition, there was a delay of the start of exponential growth at 23 °C. However, the intracellular concentration of Chl a decreased significantly due to temperature change. A significant increase in intracellular ROS was observed in coincidence with the activation of enzymatic antioxidant catalase (CAT) during the first two days of exposure to 23° and 29 °C in comparison to the control experiment, decreasing thereafter to nearly initial values. Five MCs were determined by LC-MS/MS analysis. In the experiments, the highest MC concentration, 205 fg [Leu1] MC-LR.cell− 1 expressed as MC-LR equivalent was measured in the beginning of the experiment and subsequently declined to 160 fg.cell− 1 on day 2 and 70 fg.cell− 1 on day 4 in cells exposed to 29 °C. The same trend was observed for all other MCs except for the least abundant MC-LR which showed a continuous increase during exposure time. Our results suggest a high ability of M. aeruginosa to perceive ROS and to rapidly initiate antioxidant defenses with a differential response on MC production.Fil: Giannuzzi, Leda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; ArgentinaFil: Krock, Bernd. Alfred Wegener Institute; AlemaniaFil: Crettaz Minaglia, Melina Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; ArgentinaFil: Rosso, Lorena Vanesa. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; ArgentinaFil: Houghton, Christian. Comisión Nacional de Energía Atómica; ArgentinaFil: Sedan, Daniela Yazmine. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; ArgentinaFil: Malanga, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; ArgentinaFil: Espinosa, Mariela. Comisión Nacional de Energía Atómica; ArgentinaFil: Andrinolo, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; ArgentinaFil: Hernando, Marcelo Pablo. Comisión Nacional de Energía Atómica; ArgentinaElsevier Science Inc2016-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/47439Giannuzzi, Leda; Krock, Bernd; Crettaz Minaglia, Melina Celeste; Rosso, Lorena Vanesa; Houghton, Christian; et al.; Growth, toxin production, active oxygen species and catalase activity of Microcystis aeruginosa (Cyanophyceae) exposed to temperature stress; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 189; 11-2016; 22-301532-0456CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpc.2016.07.001info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1532045616300734info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:49:55Zoai:ri.conicet.gov.ar:11336/47439instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:49:56.128CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Growth, toxin production, active oxygen species and catalase activity of Microcystis aeruginosa (Cyanophyceae) exposed to temperature stress
title Growth, toxin production, active oxygen species and catalase activity of Microcystis aeruginosa (Cyanophyceae) exposed to temperature stress
spellingShingle Growth, toxin production, active oxygen species and catalase activity of Microcystis aeruginosa (Cyanophyceae) exposed to temperature stress
Giannuzzi, Leda
Catalase
Chlorophyll
Microcystin Variants
Ros
Catalase
Chlorophyll
Ros
title_short Growth, toxin production, active oxygen species and catalase activity of Microcystis aeruginosa (Cyanophyceae) exposed to temperature stress
title_full Growth, toxin production, active oxygen species and catalase activity of Microcystis aeruginosa (Cyanophyceae) exposed to temperature stress
title_fullStr Growth, toxin production, active oxygen species and catalase activity of Microcystis aeruginosa (Cyanophyceae) exposed to temperature stress
title_full_unstemmed Growth, toxin production, active oxygen species and catalase activity of Microcystis aeruginosa (Cyanophyceae) exposed to temperature stress
title_sort Growth, toxin production, active oxygen species and catalase activity of Microcystis aeruginosa (Cyanophyceae) exposed to temperature stress
dc.creator.none.fl_str_mv Giannuzzi, Leda
Krock, Bernd
Crettaz Minaglia, Melina Celeste
Rosso, Lorena Vanesa
Houghton, Christian
Sedan, Daniela Yazmine
Malanga, Gabriela Fabiana
Espinosa, Mariela
Andrinolo, Dario
Hernando, Marcelo Pablo
author Giannuzzi, Leda
author_facet Giannuzzi, Leda
Krock, Bernd
Crettaz Minaglia, Melina Celeste
Rosso, Lorena Vanesa
Houghton, Christian
Sedan, Daniela Yazmine
Malanga, Gabriela Fabiana
Espinosa, Mariela
Andrinolo, Dario
Hernando, Marcelo Pablo
author_role author
author2 Krock, Bernd
Crettaz Minaglia, Melina Celeste
Rosso, Lorena Vanesa
Houghton, Christian
Sedan, Daniela Yazmine
Malanga, Gabriela Fabiana
Espinosa, Mariela
Andrinolo, Dario
Hernando, Marcelo Pablo
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Catalase
Chlorophyll
Microcystin Variants
Ros
Catalase
Chlorophyll
Ros
topic Catalase
Chlorophyll
Microcystin Variants
Ros
Catalase
Chlorophyll
Ros
purl_subject.fl_str_mv https://purl.org/becyt/ford/3.1
https://purl.org/becyt/ford/3
dc.description.none.fl_txt_mv Microcystis are known for their potential ability to synthesize toxins, mainly microcystins (MCs). In order to evaluate the effects of temperature on chlorophyll a (Chl a), growth, physiological responses and toxin production of a native Microcystis aeruginosa, we exposed the cells to low (23 °C) and high (29 °C) temperature in addition to a 26 °C control treatment. Exponential growth rate was significantly higher at 29 °C compared to 23 °C and control, reaching 0.43, 0.32 and 0.33 day−1 respectively. In addition, there was a delay of the start of exponential growth at 23 °C. However, the intracellular concentration of Chl a decreased significantly due to temperature change. A significant increase in intracellular ROS was observed in coincidence with the activation of enzymatic antioxidant catalase (CAT) during the first two days of exposure to 23° and 29 °C in comparison to the control experiment, decreasing thereafter to nearly initial values. Five MCs were determined by LC-MS/MS analysis. In the experiments, the highest MC concentration, 205 fg [Leu1] MC-LR.cell− 1 expressed as MC-LR equivalent was measured in the beginning of the experiment and subsequently declined to 160 fg.cell− 1 on day 2 and 70 fg.cell− 1 on day 4 in cells exposed to 29 °C. The same trend was observed for all other MCs except for the least abundant MC-LR which showed a continuous increase during exposure time. Our results suggest a high ability of M. aeruginosa to perceive ROS and to rapidly initiate antioxidant defenses with a differential response on MC production.
Fil: Giannuzzi, Leda. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; Argentina
Fil: Krock, Bernd. Alfred Wegener Institute; Alemania
Fil: Crettaz Minaglia, Melina Celeste. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; Argentina
Fil: Rosso, Lorena Vanesa. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; Argentina
Fil: Houghton, Christian. Comisión Nacional de Energía Atómica; Argentina
Fil: Sedan, Daniela Yazmine. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; Argentina
Fil: Malanga, Gabriela Fabiana. Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Houssay. Instituto de Bioquímica y Medicina Molecular. Universidad de Buenos Aires. Facultad Medicina. Instituto de Bioquímica y Medicina Molecular; Argentina
Fil: Espinosa, Mariela. Comisión Nacional de Energía Atómica; Argentina
Fil: Andrinolo, Dario. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad Nacional de La Plata. Facultad de Ciencias Exactas. Departamento de Ciencias Biológicas. Área de Toxicología; Argentina
Fil: Hernando, Marcelo Pablo. Comisión Nacional de Energía Atómica; Argentina
description Microcystis are known for their potential ability to synthesize toxins, mainly microcystins (MCs). In order to evaluate the effects of temperature on chlorophyll a (Chl a), growth, physiological responses and toxin production of a native Microcystis aeruginosa, we exposed the cells to low (23 °C) and high (29 °C) temperature in addition to a 26 °C control treatment. Exponential growth rate was significantly higher at 29 °C compared to 23 °C and control, reaching 0.43, 0.32 and 0.33 day−1 respectively. In addition, there was a delay of the start of exponential growth at 23 °C. However, the intracellular concentration of Chl a decreased significantly due to temperature change. A significant increase in intracellular ROS was observed in coincidence with the activation of enzymatic antioxidant catalase (CAT) during the first two days of exposure to 23° and 29 °C in comparison to the control experiment, decreasing thereafter to nearly initial values. Five MCs were determined by LC-MS/MS analysis. In the experiments, the highest MC concentration, 205 fg [Leu1] MC-LR.cell− 1 expressed as MC-LR equivalent was measured in the beginning of the experiment and subsequently declined to 160 fg.cell− 1 on day 2 and 70 fg.cell− 1 on day 4 in cells exposed to 29 °C. The same trend was observed for all other MCs except for the least abundant MC-LR which showed a continuous increase during exposure time. Our results suggest a high ability of M. aeruginosa to perceive ROS and to rapidly initiate antioxidant defenses with a differential response on MC production.
publishDate 2016
dc.date.none.fl_str_mv 2016-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/47439
Giannuzzi, Leda; Krock, Bernd; Crettaz Minaglia, Melina Celeste; Rosso, Lorena Vanesa; Houghton, Christian; et al.; Growth, toxin production, active oxygen species and catalase activity of Microcystis aeruginosa (Cyanophyceae) exposed to temperature stress; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 189; 11-2016; 22-30
1532-0456
CONICET Digital
CONICET
url http://hdl.handle.net/11336/47439
identifier_str_mv Giannuzzi, Leda; Krock, Bernd; Crettaz Minaglia, Melina Celeste; Rosso, Lorena Vanesa; Houghton, Christian; et al.; Growth, toxin production, active oxygen species and catalase activity of Microcystis aeruginosa (Cyanophyceae) exposed to temperature stress; Elsevier Science Inc; Comparative Biochemistry And Physiology. Toxicology & Pharmacology; 189; 11-2016; 22-30
1532-0456
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.cbpc.2016.07.001
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1532045616300734
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science Inc
publisher.none.fl_str_mv Elsevier Science Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.13397

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