UV-resistant Acinetobacter sp. isolates from Andean wetlands display high catalase activity

Autores
Di Capua, Cecilia Beatriz; Bortolotti, Ana; Farias, Maria Eugenia; Cortez, Nestor Ricardo
Año de publicación
2011
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Andean wetlands are characterized by their extreme environmental conditions such as high UV radiation, elevated heavy metal content and salinity. We present here the first study on UV tolerance and antioxidant defense of four Acinetobacter strains: Ver3, Ver5 and Ver7, isolated from Lake Verde, and N40 from Lake Negra, both lakes located 4400m above sea level. All four isolates displayed higher UV resistance compared with collection strains, with Ver3 and Ver7 being the most tolerant strains not only to UV radiation but also to hydrogen peroxide (H2O2) and methyl viologen (MV) challenges. A single superoxide dismutase band with similar activity was detected in all studied strains, whereas different electrophoretic pattern and activity levels were observed for catalase. Ver3 and Ver7 displayed 5-15 times higher catalase activity levels than the control strains. Analysis of the response of antioxidant enzymes to UV and oxidative challenges revealed a significant increase in Ver7 catalase activity after H2O2 and MV exposure. Incubation of Ver7 cultures with a catalase inhibitor resulted in a significant decrease of tolerance against UV radiation. We conclude that the high catalase activity displayed by Ver7 isolate could play an important role in UV tolerance.
Fil: Di Capua, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Bortolotti, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Fil: Cortez, Nestor Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Materia
Acinetobacter
Andean Wetlands
Catalase
Oxidative Stress
Superoxide Dismutase
Uv Radiation
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/37868
Acceder
id CONICETDig_2cf93876d4c6272b1f8bf0bbf67f42ef
oai_identifier_str oai:ri.conicet.gov.ar:11336/37868
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling UV-resistant Acinetobacter sp. isolates from Andean wetlands display high catalase activityDi Capua, Cecilia BeatrizBortolotti, AnaFarias, Maria EugeniaCortez, Nestor RicardoAcinetobacterAndean WetlandsCatalaseOxidative StressSuperoxide DismutaseUv Radiationhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1Andean wetlands are characterized by their extreme environmental conditions such as high UV radiation, elevated heavy metal content and salinity. We present here the first study on UV tolerance and antioxidant defense of four Acinetobacter strains: Ver3, Ver5 and Ver7, isolated from Lake Verde, and N40 from Lake Negra, both lakes located 4400m above sea level. All four isolates displayed higher UV resistance compared with collection strains, with Ver3 and Ver7 being the most tolerant strains not only to UV radiation but also to hydrogen peroxide (H2O2) and methyl viologen (MV) challenges. A single superoxide dismutase band with similar activity was detected in all studied strains, whereas different electrophoretic pattern and activity levels were observed for catalase. Ver3 and Ver7 displayed 5-15 times higher catalase activity levels than the control strains. Analysis of the response of antioxidant enzymes to UV and oxidative challenges revealed a significant increase in Ver7 catalase activity after H2O2 and MV exposure. Incubation of Ver7 cultures with a catalase inhibitor resulted in a significant decrease of tolerance against UV radiation. We conclude that the high catalase activity displayed by Ver7 isolate could play an important role in UV tolerance.Fil: Di Capua, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Bortolotti, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaFil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; ArgentinaFil: Cortez, Nestor Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; ArgentinaWiley Blackwell Publishing, Inc2011-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/37868Di Capua, Cecilia Beatriz; Bortolotti, Ana; Farias, Maria Eugenia; Cortez, Nestor Ricardo; UV-resistant Acinetobacter sp. isolates from Andean wetlands display high catalase activity; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 317; 2; 4-2011; 181-1890378-1097CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2011.02231.xinfo:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/317/2/181/623976info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2011.02231.x/abstractinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-03T09:54:11Zoai:ri.conicet.gov.ar:11336/37868instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-03 09:54:11.342CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv UV-resistant Acinetobacter sp. isolates from Andean wetlands display high catalase activity
title UV-resistant Acinetobacter sp. isolates from Andean wetlands display high catalase activity
spellingShingle UV-resistant Acinetobacter sp. isolates from Andean wetlands display high catalase activity
Di Capua, Cecilia Beatriz
Acinetobacter
Andean Wetlands
Catalase
Oxidative Stress
Superoxide Dismutase
Uv Radiation
title_short UV-resistant Acinetobacter sp. isolates from Andean wetlands display high catalase activity
title_full UV-resistant Acinetobacter sp. isolates from Andean wetlands display high catalase activity
title_fullStr UV-resistant Acinetobacter sp. isolates from Andean wetlands display high catalase activity
title_full_unstemmed UV-resistant Acinetobacter sp. isolates from Andean wetlands display high catalase activity
title_sort UV-resistant Acinetobacter sp. isolates from Andean wetlands display high catalase activity
dc.creator.none.fl_str_mv Di Capua, Cecilia Beatriz
Bortolotti, Ana
Farias, Maria Eugenia
Cortez, Nestor Ricardo
author Di Capua, Cecilia Beatriz
author_facet Di Capua, Cecilia Beatriz
Bortolotti, Ana
Farias, Maria Eugenia
Cortez, Nestor Ricardo
author_role author
author2 Bortolotti, Ana
Farias, Maria Eugenia
Cortez, Nestor Ricardo
author2_role author
author
author
dc.subject.none.fl_str_mv Acinetobacter
Andean Wetlands
Catalase
Oxidative Stress
Superoxide Dismutase
Uv Radiation
topic Acinetobacter
Andean Wetlands
Catalase
Oxidative Stress
Superoxide Dismutase
Uv Radiation
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv Andean wetlands are characterized by their extreme environmental conditions such as high UV radiation, elevated heavy metal content and salinity. We present here the first study on UV tolerance and antioxidant defense of four Acinetobacter strains: Ver3, Ver5 and Ver7, isolated from Lake Verde, and N40 from Lake Negra, both lakes located 4400m above sea level. All four isolates displayed higher UV resistance compared with collection strains, with Ver3 and Ver7 being the most tolerant strains not only to UV radiation but also to hydrogen peroxide (H2O2) and methyl viologen (MV) challenges. A single superoxide dismutase band with similar activity was detected in all studied strains, whereas different electrophoretic pattern and activity levels were observed for catalase. Ver3 and Ver7 displayed 5-15 times higher catalase activity levels than the control strains. Analysis of the response of antioxidant enzymes to UV and oxidative challenges revealed a significant increase in Ver7 catalase activity after H2O2 and MV exposure. Incubation of Ver7 cultures with a catalase inhibitor resulted in a significant decrease of tolerance against UV radiation. We conclude that the high catalase activity displayed by Ver7 isolate could play an important role in UV tolerance.
Fil: Di Capua, Cecilia Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Bortolotti, Ana. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
Fil: Farias, Maria Eugenia. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Planta Piloto de Procesos Industriales Microbiologicos; Argentina
Fil: Cortez, Nestor Ricardo. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Rosario. Instituto de Biología Molecular y Celular de Rosario. Universidad Nacional de Rosario. Facultad de Ciencias Bioquímicas y Farmacéuticas. Instituto de Biología Molecular y Celular de Rosario; Argentina
description Andean wetlands are characterized by their extreme environmental conditions such as high UV radiation, elevated heavy metal content and salinity. We present here the first study on UV tolerance and antioxidant defense of four Acinetobacter strains: Ver3, Ver5 and Ver7, isolated from Lake Verde, and N40 from Lake Negra, both lakes located 4400m above sea level. All four isolates displayed higher UV resistance compared with collection strains, with Ver3 and Ver7 being the most tolerant strains not only to UV radiation but also to hydrogen peroxide (H2O2) and methyl viologen (MV) challenges. A single superoxide dismutase band with similar activity was detected in all studied strains, whereas different electrophoretic pattern and activity levels were observed for catalase. Ver3 and Ver7 displayed 5-15 times higher catalase activity levels than the control strains. Analysis of the response of antioxidant enzymes to UV and oxidative challenges revealed a significant increase in Ver7 catalase activity after H2O2 and MV exposure. Incubation of Ver7 cultures with a catalase inhibitor resulted in a significant decrease of tolerance against UV radiation. We conclude that the high catalase activity displayed by Ver7 isolate could play an important role in UV tolerance.
publishDate 2011
dc.date.none.fl_str_mv 2011-04
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/37868
Di Capua, Cecilia Beatriz; Bortolotti, Ana; Farias, Maria Eugenia; Cortez, Nestor Ricardo; UV-resistant Acinetobacter sp. isolates from Andean wetlands display high catalase activity; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 317; 2; 4-2011; 181-189
0378-1097
CONICET Digital
CONICET
url http://hdl.handle.net/11336/37868
identifier_str_mv Di Capua, Cecilia Beatriz; Bortolotti, Ana; Farias, Maria Eugenia; Cortez, Nestor Ricardo; UV-resistant Acinetobacter sp. isolates from Andean wetlands display high catalase activity; Wiley Blackwell Publishing, Inc; FEMS Microbiology Letters; 317; 2; 4-2011; 181-189
0378-1097
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1574-6968.2011.02231.x
info:eu-repo/semantics/altIdentifier/url/https://academic.oup.com/femsle/article/317/2/181/623976
info:eu-repo/semantics/altIdentifier/url/http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2011.02231.x/abstract
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
publisher.none.fl_str_mv Wiley Blackwell Publishing, Inc
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
_version_ 1842269269890433024
score 13.13397

Publicaciones similares