Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress

Autores
Causin, Humberto Fabio; Marchetti, Cintia; Pena, Liliana Beatriz; Gallego, Susana Mabel; Barneix, Atilio José
Año de publicación
2015
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
In shaded wheat (Triticum aestivum L.) leaves, the suppression of blue radiation (BR) triggers senescence. This phenomenon is correlated to an increase in oxidative stress symptoms and a decrease of catalase (CAT) activity, among other traits. Previous data suggest that the radiation signal transduction pathway may involve changes in Ca2+ and H2O2 homeostasis. For better a understanding of the interaction among the spectral composition of radiation, Ca2+ availability, and the antioxidant metabolism in the regulation of shade-induced senescence, detached wheat leaves were placed in a growth chamber and exposed to either blue (B, high BR transmittance) and/or green (G, very low BR transmittance) Lee® filters in the absence or presence of 0.8 mM verapamil (a Ca2+ channels blocker), 4.0 mM EGTA (a Ca2+chelator), or 8.0 mM 3-amino-1,2,4-triazole (a CAT inhibitor). At defined time points, the leaf samples were analyzed for changes in chlorophyll content, specific activities of CAT, ascorbate peroxidase (APX), and guaiacol peroxidase (POX), CAT isozymes, and gene expression of CAT1, CAT2, and two senescence markers (TaSAG1 and TaSAG3). BR transmittance decreased the chlorophyll degradation rate and SAG genes expression either in leaves continuously exposed under the B filter, as well as in leaves previously exposed under the G filter. The effect of BR was associated with the maintenance of a high CAT (but not APX and POX) activity, and it was suppressed either in the presence of 3-AT or when Ca2+ availability was decreased. BR altered the CAT activity both at the transcriptional and at the posttranscriptional level. Nevertheless, different responses of CAT isozymes and CAT genes expression profiles to specific treatment combinations indicate that they differed in their regulatory pathways.
Fil: Causin, Humberto Fabio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Marchetti, Cintia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Pena, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Gallego, Susana Mabel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barneix, Atilio José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; Argentina
Materia
ANTIOXIDATNTS
BLUE RADIATION
CALCIUM
GENE EXPRESSION
TRITICUM AESTIVUM
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/94509
Acceder
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oai_identifier_str oai:ri.conicet.gov.ar:11336/94509
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stressCausin, Humberto FabioMarchetti, CintiaPena, Liliana BeatrizGallego, Susana MabelBarneix, Atilio JoséANTIOXIDATNTSBLUE RADIATIONCALCIUMGENE EXPRESSIONTRITICUM AESTIVUMhttps://purl.org/becyt/ford/1.6https://purl.org/becyt/ford/1In shaded wheat (Triticum aestivum L.) leaves, the suppression of blue radiation (BR) triggers senescence. This phenomenon is correlated to an increase in oxidative stress symptoms and a decrease of catalase (CAT) activity, among other traits. Previous data suggest that the radiation signal transduction pathway may involve changes in Ca2+ and H2O2 homeostasis. For better a understanding of the interaction among the spectral composition of radiation, Ca2+ availability, and the antioxidant metabolism in the regulation of shade-induced senescence, detached wheat leaves were placed in a growth chamber and exposed to either blue (B, high BR transmittance) and/or green (G, very low BR transmittance) Lee® filters in the absence or presence of 0.8 mM verapamil (a Ca2+ channels blocker), 4.0 mM EGTA (a Ca2+chelator), or 8.0 mM 3-amino-1,2,4-triazole (a CAT inhibitor). At defined time points, the leaf samples were analyzed for changes in chlorophyll content, specific activities of CAT, ascorbate peroxidase (APX), and guaiacol peroxidase (POX), CAT isozymes, and gene expression of CAT1, CAT2, and two senescence markers (TaSAG1 and TaSAG3). BR transmittance decreased the chlorophyll degradation rate and SAG genes expression either in leaves continuously exposed under the B filter, as well as in leaves previously exposed under the G filter. The effect of BR was associated with the maintenance of a high CAT (but not APX and POX) activity, and it was suppressed either in the presence of 3-AT or when Ca2+ availability was decreased. BR altered the CAT activity both at the transcriptional and at the posttranscriptional level. Nevertheless, different responses of CAT isozymes and CAT genes expression profiles to specific treatment combinations indicate that they differed in their regulatory pathways.Fil: Causin, Humberto Fabio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Marchetti, Cintia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; ArgentinaFil: Pena, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; ArgentinaFil: Gallego, Susana Mabel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; ArgentinaFil: Barneix, Atilio José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; ArgentinaSpringer2015-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/94509Causin, Humberto Fabio; Marchetti, Cintia; Pena, Liliana Beatriz; Gallego, Susana Mabel; Barneix, Atilio José; Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress; Springer; Biologia Plantarum; 59; 1; 1-2015; 154-1620006-3134CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1007/s10535-014-0480-zinfo:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10535-014-0480-zinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T09:56:02Zoai:ri.conicet.gov.ar:11336/94509instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 09:56:02.383CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
title Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
spellingShingle Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
Causin, Humberto Fabio
ANTIOXIDATNTS
BLUE RADIATION
CALCIUM
GENE EXPRESSION
TRITICUM AESTIVUM
title_short Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
title_full Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
title_fullStr Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
title_full_unstemmed Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
title_sort Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress
dc.creator.none.fl_str_mv Causin, Humberto Fabio
Marchetti, Cintia
Pena, Liliana Beatriz
Gallego, Susana Mabel
Barneix, Atilio José
author Causin, Humberto Fabio
author_facet Causin, Humberto Fabio
Marchetti, Cintia
Pena, Liliana Beatriz
Gallego, Susana Mabel
Barneix, Atilio José
author_role author
author2 Marchetti, Cintia
Pena, Liliana Beatriz
Gallego, Susana Mabel
Barneix, Atilio José
author2_role author
author
author
author
dc.subject.none.fl_str_mv ANTIOXIDATNTS
BLUE RADIATION
CALCIUM
GENE EXPRESSION
TRITICUM AESTIVUM
topic ANTIOXIDATNTS
BLUE RADIATION
CALCIUM
GENE EXPRESSION
TRITICUM AESTIVUM
purl_subject.fl_str_mv https://purl.org/becyt/ford/1.6
https://purl.org/becyt/ford/1
dc.description.none.fl_txt_mv In shaded wheat (Triticum aestivum L.) leaves, the suppression of blue radiation (BR) triggers senescence. This phenomenon is correlated to an increase in oxidative stress symptoms and a decrease of catalase (CAT) activity, among other traits. Previous data suggest that the radiation signal transduction pathway may involve changes in Ca2+ and H2O2 homeostasis. For better a understanding of the interaction among the spectral composition of radiation, Ca2+ availability, and the antioxidant metabolism in the regulation of shade-induced senescence, detached wheat leaves were placed in a growth chamber and exposed to either blue (B, high BR transmittance) and/or green (G, very low BR transmittance) Lee® filters in the absence or presence of 0.8 mM verapamil (a Ca2+ channels blocker), 4.0 mM EGTA (a Ca2+chelator), or 8.0 mM 3-amino-1,2,4-triazole (a CAT inhibitor). At defined time points, the leaf samples were analyzed for changes in chlorophyll content, specific activities of CAT, ascorbate peroxidase (APX), and guaiacol peroxidase (POX), CAT isozymes, and gene expression of CAT1, CAT2, and two senescence markers (TaSAG1 and TaSAG3). BR transmittance decreased the chlorophyll degradation rate and SAG genes expression either in leaves continuously exposed under the B filter, as well as in leaves previously exposed under the G filter. The effect of BR was associated with the maintenance of a high CAT (but not APX and POX) activity, and it was suppressed either in the presence of 3-AT or when Ca2+ availability was decreased. BR altered the CAT activity both at the transcriptional and at the posttranscriptional level. Nevertheless, different responses of CAT isozymes and CAT genes expression profiles to specific treatment combinations indicate that they differed in their regulatory pathways.
Fil: Causin, Humberto Fabio. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Marchetti, Cintia. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina
Fil: Pena, Liliana Beatriz. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina
Fil: Gallego, Susana Mabel. Universidad de Buenos Aires. Facultad de Farmacia y Bioquímica; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina
Fil: Barneix, Atilio José. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación de Recursos Naturales. Instituto de Suelos; Argentina
description In shaded wheat (Triticum aestivum L.) leaves, the suppression of blue radiation (BR) triggers senescence. This phenomenon is correlated to an increase in oxidative stress symptoms and a decrease of catalase (CAT) activity, among other traits. Previous data suggest that the radiation signal transduction pathway may involve changes in Ca2+ and H2O2 homeostasis. For better a understanding of the interaction among the spectral composition of radiation, Ca2+ availability, and the antioxidant metabolism in the regulation of shade-induced senescence, detached wheat leaves were placed in a growth chamber and exposed to either blue (B, high BR transmittance) and/or green (G, very low BR transmittance) Lee® filters in the absence or presence of 0.8 mM verapamil (a Ca2+ channels blocker), 4.0 mM EGTA (a Ca2+chelator), or 8.0 mM 3-amino-1,2,4-triazole (a CAT inhibitor). At defined time points, the leaf samples were analyzed for changes in chlorophyll content, specific activities of CAT, ascorbate peroxidase (APX), and guaiacol peroxidase (POX), CAT isozymes, and gene expression of CAT1, CAT2, and two senescence markers (TaSAG1 and TaSAG3). BR transmittance decreased the chlorophyll degradation rate and SAG genes expression either in leaves continuously exposed under the B filter, as well as in leaves previously exposed under the G filter. The effect of BR was associated with the maintenance of a high CAT (but not APX and POX) activity, and it was suppressed either in the presence of 3-AT or when Ca2+ availability was decreased. BR altered the CAT activity both at the transcriptional and at the posttranscriptional level. Nevertheless, different responses of CAT isozymes and CAT genes expression profiles to specific treatment combinations indicate that they differed in their regulatory pathways.
publishDate 2015
dc.date.none.fl_str_mv 2015-01
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/94509
Causin, Humberto Fabio; Marchetti, Cintia; Pena, Liliana Beatriz; Gallego, Susana Mabel; Barneix, Atilio José; Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress; Springer; Biologia Plantarum; 59; 1; 1-2015; 154-162
0006-3134
CONICET Digital
CONICET
url http://hdl.handle.net/11336/94509
identifier_str_mv Causin, Humberto Fabio; Marchetti, Cintia; Pena, Liliana Beatriz; Gallego, Susana Mabel; Barneix, Atilio José; Down-regulation of catalase activity contributes to senescence induction in wheat leaves exposed to shading stress; Springer; Biologia Plantarum; 59; 1; 1-2015; 154-162
0006-3134
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1007/s10535-014-0480-z
info:eu-repo/semantics/altIdentifier/url/https://link.springer.com/article/10.1007/s10535-014-0480-z
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
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eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
application/pdf
application/pdf
application/pdf
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dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
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reponame_str CONICET Digital (CONICET)
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instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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