Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay

Autores
Castells, Matias; Caffarena, Rubén Darío; Casaux, María Laura; Schild, Carlos; Castells, Felipe; Castells, Daniel; Victoria, Matías; Riet‑Correa, Franklin; Giannitti, Federico; Parreño, Viviana; Colina, Rodney
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Uruguay is a leading exporter of bovine meat and dairy products, and cattle production is one of the principal economic backbones in this country. A main clinical problem faced by livestock farmers is neonatal calf diarrhea (NCD); however, causes of NCD have not been extensively studied in Uruguay. Bovine norovirus (BoNoV) has been proposed as one of the possible etiologies of NCD as experimentally infected calves developed diarrhea and enteropathy, although limited information is available from field surveys. The aims of this study were to determine the frequency of infection, to investigate possible risk factors, and to determine the molecular diversity of BoNoV in Uruguay. A total of 761 samples of feces or intestinal contents from dairy and beef calves were analyzed through RT-qPCR. The overall frequency of detection of BoNoV was 66.1% with higher frequency in dairy (70.5%) than beef (15.9%) calves (p < 0.01). BoNoV was detected similarly in diarrheic (78.8%) and non-diarrheic (76.2%) dairy calves (p = 0.50). Calves ≤2 weeks of age (84%) were infected more often than older (62.7%) calves (p < 0.01). Phylogenetic analysis confirmed the presence of GIII.1 and GIII.2 genotypes. In addition, we reported the circulation of recombinant strains and the detection of a strain with the recently described novel VP1 genotype. This study represents the first report describing the circulation, the associated risk factors, and the molecular diversity of BoNoV in Uruguay.
Instituto de Virología
Fil: Castells, Matías. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay.
Fil: Castells, Matías. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay
Fil: Caffarena, Rubén Darío. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay
Fil: Caffarena, Rubén Darío. Universidad de la República. Facultad de Veterinaria; Uruguay.
Fil: Casaux, María Laura. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay
Fil: Schild, Carlos. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay
Fil: Castells, Felipe. Profesional independiente; Uruguay
Fil: Castells, Daniel. Secretariado Uruguayo de la Lana. Centro de Investigación y Experimentación Dr. Alejandro Gallinal; Uruguay.
Fil: Victoria, Matías. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay.
Fil: Riet‑Correa, Franklin. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay
Fil: Giannitti, Federico. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.
Fil: Colina, Rodney. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay.
Fuente
Infection, Genetics and Evolution 86 : 104613 (December 2020)
Materia
Ganado Bovino
Virus de los Animales
Genotipos
Uruguay
Cattle
Animal Viruses
Genotypes
Norovirus Bovino
Bovine Norovirus
Nivel de accesibilidad
acceso abierto
Condiciones de uso
http://creativecommons.org/licenses/by-nc-sa/4.0/
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/11125
Acceder
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oai_identifier_str oai:localhost:20.500.12123/11125
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Detection, risk factors and molecular diversity of norovirus GIII in cattle in UruguayCastells, MatiasCaffarena, Rubén DaríoCasaux, María LauraSchild, CarlosCastells, FelipeCastells, DanielVictoria, MatíasRiet‑Correa, FranklinGiannitti, FedericoParreño, VivianaColina, RodneyGanado BovinoVirus de los AnimalesGenotiposUruguayCattleAnimal VirusesGenotypesNorovirus BovinoBovine NorovirusUruguay is a leading exporter of bovine meat and dairy products, and cattle production is one of the principal economic backbones in this country. A main clinical problem faced by livestock farmers is neonatal calf diarrhea (NCD); however, causes of NCD have not been extensively studied in Uruguay. Bovine norovirus (BoNoV) has been proposed as one of the possible etiologies of NCD as experimentally infected calves developed diarrhea and enteropathy, although limited information is available from field surveys. The aims of this study were to determine the frequency of infection, to investigate possible risk factors, and to determine the molecular diversity of BoNoV in Uruguay. A total of 761 samples of feces or intestinal contents from dairy and beef calves were analyzed through RT-qPCR. The overall frequency of detection of BoNoV was 66.1% with higher frequency in dairy (70.5%) than beef (15.9%) calves (p < 0.01). BoNoV was detected similarly in diarrheic (78.8%) and non-diarrheic (76.2%) dairy calves (p = 0.50). Calves ≤2 weeks of age (84%) were infected more often than older (62.7%) calves (p < 0.01). Phylogenetic analysis confirmed the presence of GIII.1 and GIII.2 genotypes. In addition, we reported the circulation of recombinant strains and the detection of a strain with the recently described novel VP1 genotype. This study represents the first report describing the circulation, the associated risk factors, and the molecular diversity of BoNoV in Uruguay.Instituto de VirologíaFil: Castells, Matías. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay.Fil: Castells, Matías. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; UruguayFil: Caffarena, Rubén Darío. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; UruguayFil: Caffarena, Rubén Darío. Universidad de la República. Facultad de Veterinaria; Uruguay.Fil: Casaux, María Laura. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; UruguayFil: Schild, Carlos. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; UruguayFil: Castells, Felipe. Profesional independiente; UruguayFil: Castells, Daniel. Secretariado Uruguayo de la Lana. Centro de Investigación y Experimentación Dr. Alejandro Gallinal; Uruguay.Fil: Victoria, Matías. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay.Fil: Riet‑Correa, Franklin. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; UruguayFil: Giannitti, Federico. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; UruguayFil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.Fil: Colina, Rodney. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay.Elsevier2022-01-14T13:55:17Z2022-01-14T13:55:17Z2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/11125https://www.sciencedirect.com/science/article/abs/pii/S15671348203044451567-1348https://doi.org/10.1016/j.meegid.2020.104613Infection, Genetics and Evolution 86 : 104613 (December 2020)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología Agropecuariaenginfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc-sa/4.0/Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)2025-09-29T13:45:27Zoai:localhost:20.500.12123/11125instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:45:27.82INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
title Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
spellingShingle Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
Castells, Matias
Ganado Bovino
Virus de los Animales
Genotipos
Uruguay
Cattle
Animal Viruses
Genotypes
Norovirus Bovino
Bovine Norovirus
title_short Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
title_full Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
title_fullStr Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
title_full_unstemmed Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
title_sort Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
dc.creator.none.fl_str_mv Castells, Matias
Caffarena, Rubén Darío
Casaux, María Laura
Schild, Carlos
Castells, Felipe
Castells, Daniel
Victoria, Matías
Riet‑Correa, Franklin
Giannitti, Federico
Parreño, Viviana
Colina, Rodney
author Castells, Matias
author_facet Castells, Matias
Caffarena, Rubén Darío
Casaux, María Laura
Schild, Carlos
Castells, Felipe
Castells, Daniel
Victoria, Matías
Riet‑Correa, Franklin
Giannitti, Federico
Parreño, Viviana
Colina, Rodney
author_role author
author2 Caffarena, Rubén Darío
Casaux, María Laura
Schild, Carlos
Castells, Felipe
Castells, Daniel
Victoria, Matías
Riet‑Correa, Franklin
Giannitti, Federico
Parreño, Viviana
Colina, Rodney
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Ganado Bovino
Virus de los Animales
Genotipos
Uruguay
Cattle
Animal Viruses
Genotypes
Norovirus Bovino
Bovine Norovirus
topic Ganado Bovino
Virus de los Animales
Genotipos
Uruguay
Cattle
Animal Viruses
Genotypes
Norovirus Bovino
Bovine Norovirus
dc.description.none.fl_txt_mv Uruguay is a leading exporter of bovine meat and dairy products, and cattle production is one of the principal economic backbones in this country. A main clinical problem faced by livestock farmers is neonatal calf diarrhea (NCD); however, causes of NCD have not been extensively studied in Uruguay. Bovine norovirus (BoNoV) has been proposed as one of the possible etiologies of NCD as experimentally infected calves developed diarrhea and enteropathy, although limited information is available from field surveys. The aims of this study were to determine the frequency of infection, to investigate possible risk factors, and to determine the molecular diversity of BoNoV in Uruguay. A total of 761 samples of feces or intestinal contents from dairy and beef calves were analyzed through RT-qPCR. The overall frequency of detection of BoNoV was 66.1% with higher frequency in dairy (70.5%) than beef (15.9%) calves (p < 0.01). BoNoV was detected similarly in diarrheic (78.8%) and non-diarrheic (76.2%) dairy calves (p = 0.50). Calves ≤2 weeks of age (84%) were infected more often than older (62.7%) calves (p < 0.01). Phylogenetic analysis confirmed the presence of GIII.1 and GIII.2 genotypes. In addition, we reported the circulation of recombinant strains and the detection of a strain with the recently described novel VP1 genotype. This study represents the first report describing the circulation, the associated risk factors, and the molecular diversity of BoNoV in Uruguay.
Instituto de Virología
Fil: Castells, Matías. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay.
Fil: Castells, Matías. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay
Fil: Caffarena, Rubén Darío. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay
Fil: Caffarena, Rubén Darío. Universidad de la República. Facultad de Veterinaria; Uruguay.
Fil: Casaux, María Laura. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay
Fil: Schild, Carlos. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay
Fil: Castells, Felipe. Profesional independiente; Uruguay
Fil: Castells, Daniel. Secretariado Uruguayo de la Lana. Centro de Investigación y Experimentación Dr. Alejandro Gallinal; Uruguay.
Fil: Victoria, Matías. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay.
Fil: Riet‑Correa, Franklin. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay
Fil: Giannitti, Federico. Instituto Nacional de Investigación Agropecuaria (INIA). Estación Experimental La Estanzuela; Uruguay
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina.
Fil: Colina, Rodney. Universidad de la República. Centro Universitario de Salto. CENUR Litoral Norte. Laboratorio de Virología Molecular; Uruguay.
description Uruguay is a leading exporter of bovine meat and dairy products, and cattle production is one of the principal economic backbones in this country. A main clinical problem faced by livestock farmers is neonatal calf diarrhea (NCD); however, causes of NCD have not been extensively studied in Uruguay. Bovine norovirus (BoNoV) has been proposed as one of the possible etiologies of NCD as experimentally infected calves developed diarrhea and enteropathy, although limited information is available from field surveys. The aims of this study were to determine the frequency of infection, to investigate possible risk factors, and to determine the molecular diversity of BoNoV in Uruguay. A total of 761 samples of feces or intestinal contents from dairy and beef calves were analyzed through RT-qPCR. The overall frequency of detection of BoNoV was 66.1% with higher frequency in dairy (70.5%) than beef (15.9%) calves (p < 0.01). BoNoV was detected similarly in diarrheic (78.8%) and non-diarrheic (76.2%) dairy calves (p = 0.50). Calves ≤2 weeks of age (84%) were infected more often than older (62.7%) calves (p < 0.01). Phylogenetic analysis confirmed the presence of GIII.1 and GIII.2 genotypes. In addition, we reported the circulation of recombinant strains and the detection of a strain with the recently described novel VP1 genotype. This study represents the first report describing the circulation, the associated risk factors, and the molecular diversity of BoNoV in Uruguay.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
2022-01-14T13:55:17Z
2022-01-14T13:55:17Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/11125
https://www.sciencedirect.com/science/article/abs/pii/S1567134820304445
1567-1348
https://doi.org/10.1016/j.meegid.2020.104613
url http://hdl.handle.net/20.500.12123/11125
https://www.sciencedirect.com/science/article/abs/pii/S1567134820304445
https://doi.org/10.1016/j.meegid.2020.104613
identifier_str_mv 1567-1348
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by-nc-sa/4.0/
Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv Infection, Genetics and Evolution 86 : 104613 (December 2020)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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