Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype

Autores
Ferragut, Fátima; Vega, Celina Guadalupe; Mauroy, Axel; Conceição-Neto, Nádia; Zeller, Mark; Heylen, Elisabeth; Louge Uriarte, Enrique Leopoldo; Bilbao, Gladys; Bok, Marina; Matthijnssens, Jelle; Thiry, Etienne; Badaracco, Alejandra; Parreño, Gladys Viviana
Año de publicación
2016
Idioma
inglés
Tipo de recurso
artículo
Estado
versión aceptada
Descripción
Bovine noroviruses are enteric pathogens detected in fecal samples of both diarrheic and non-diarrheic calves from several countries worldwide. However, epidemiological information regarding bovine noroviruses is still lacking for many important cattle producing countries from South America. In this study, three bovine norovirus genogroup III sequences were determined by conventional RT-PCR and Sanger sequencing in feces from diarrheic dairy calves from Argentina (B4836, B4848, and B4881, all collected in 2012). Phylogenetic studies based on a partial coding region for the RNA-dependent RNA polymerase (RdRp, 503 nucleotides) of these three samples suggested that two of them (B4836 and B4881) belong to genotype 2 (GIII.2) while the third one (B4848) was more closely related to genotype 1 (GIII.1) strains. By deep sequencing, the capsid region from two of these strains could be determined. This confirmed the circulation of genotype 1 (B4848) together with the presence of another sequence (B4881) sharing its highest genetic relatedness with genotype 1, but sufficiently distant to constitute a new genotype. This latter strain was shown in silico to be a recombinant: phylogenetic divergence was detected between its RNA-dependent RNA polymerase coding sequence (genotype GIII.2) and its capsid protein coding sequence (genotype GIII.1 or a potential norovirus genotype). According to this data, this strain could be the second genotype GIII.2_GIII.1 bovine norovirus recombinant described in literature worldwide. Further analysis suggested that this strain could even be a potential norovirus GIII genotype, tentatively named GIII.4. The data provides important epidemiological and evolutionary information on bovine noroviruses circulating in South America
Inst.de Virología
Fil: Ferragut, Fátima. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Mauroy, Axel. University of Liège. Faculty of Veterinary Medicine. Fundamental and Applied Research for Animal and Health Centre. Department of Infectious and Parasitic Diseases. Veterinary Virology and Animal Viral Diseases; Bélgica
Fil: Conceição-Neto, Nádia. University of Leuven. Department of Microbiology and Immunology; Bélgica. Rega Institute for Medical Research, Laboratory of Viral Metagenomics; Bélgica
Fil: Zeller, Mark. University of Leuven. Department of Microbiology and Immunology. Rega Institute for Medical Research, Laboratory of Viral Metagenomics; Bélgica
Fil: Heylen, Elisabeth. University of Leuven. Department of Microbiology and Immunology. Rega Institute for Medical Research, Laboratory of Viral Metagenomics; Bélgica
Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Area de Producción Animal; Argentina
Fil: Bilbao, Gladys. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela de Veterinaria; Argentina
Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Matthijnssens, Jelle. University of Leuven. Department of Microbiology and Immunology; Bélgica. Rega Institute for Medical Research. Laboratory of Viral Metagenomics; Bélgica.
Fil: Thiry, Etienne. University of Liège. Faculty of Veterinary Medicine. Fundamental and Applied Research for Animal and Health Centre. Department of Infectious and Parasitic Diseases. Veterinary Virology and Animal Viral Diseases; Bélgica
Fil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fuente
Infection, genetics and evolution 40 : 144-150. (June 2016)
Materia
Enfermedades de los Animales
Genotipos
Virus
Ganado de Leche
Animal Diseases
Genotypes
Viruses
Dairy Cattle
Norovirus
Nivel de accesibilidad
acceso restringido
Condiciones de uso
Repositorio
INTA Digital (INTA)
Institución
Instituto Nacional de Tecnología Agropecuaria
OAI Identificador
oai:localhost:20.500.12123/1219
Acceder
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oai_identifier_str oai:localhost:20.500.12123/1219
network_acronym_str INTADig
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network_name_str INTA Digital (INTA)
spelling Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotypeFerragut, FátimaVega, Celina GuadalupeMauroy, AxelConceição-Neto, NádiaZeller, MarkHeylen, ElisabethLouge Uriarte, Enrique LeopoldoBilbao, GladysBok, MarinaMatthijnssens, JelleThiry, EtienneBadaracco, AlejandraParreño, Gladys VivianaEnfermedades de los AnimalesGenotiposVirusGanado de LecheAnimal DiseasesGenotypesVirusesDairy CattleNorovirusBovine noroviruses are enteric pathogens detected in fecal samples of both diarrheic and non-diarrheic calves from several countries worldwide. However, epidemiological information regarding bovine noroviruses is still lacking for many important cattle producing countries from South America. In this study, three bovine norovirus genogroup III sequences were determined by conventional RT-PCR and Sanger sequencing in feces from diarrheic dairy calves from Argentina (B4836, B4848, and B4881, all collected in 2012). Phylogenetic studies based on a partial coding region for the RNA-dependent RNA polymerase (RdRp, 503 nucleotides) of these three samples suggested that two of them (B4836 and B4881) belong to genotype 2 (GIII.2) while the third one (B4848) was more closely related to genotype 1 (GIII.1) strains. By deep sequencing, the capsid region from two of these strains could be determined. This confirmed the circulation of genotype 1 (B4848) together with the presence of another sequence (B4881) sharing its highest genetic relatedness with genotype 1, but sufficiently distant to constitute a new genotype. This latter strain was shown in silico to be a recombinant: phylogenetic divergence was detected between its RNA-dependent RNA polymerase coding sequence (genotype GIII.2) and its capsid protein coding sequence (genotype GIII.1 or a potential norovirus genotype). According to this data, this strain could be the second genotype GIII.2_GIII.1 bovine norovirus recombinant described in literature worldwide. Further analysis suggested that this strain could even be a potential norovirus GIII genotype, tentatively named GIII.4. The data provides important epidemiological and evolutionary information on bovine noroviruses circulating in South AmericaInst.de VirologíaFil: Ferragut, Fátima. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Mauroy, Axel. University of Liège. Faculty of Veterinary Medicine. Fundamental and Applied Research for Animal and Health Centre. Department of Infectious and Parasitic Diseases. Veterinary Virology and Animal Viral Diseases; BélgicaFil: Conceição-Neto, Nádia. University of Leuven. Department of Microbiology and Immunology; Bélgica. Rega Institute for Medical Research, Laboratory of Viral Metagenomics; BélgicaFil: Zeller, Mark. University of Leuven. Department of Microbiology and Immunology. Rega Institute for Medical Research, Laboratory of Viral Metagenomics; BélgicaFil: Heylen, Elisabeth. University of Leuven. Department of Microbiology and Immunology. Rega Institute for Medical Research, Laboratory of Viral Metagenomics; BélgicaFil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Area de Producción Animal; ArgentinaFil: Bilbao, Gladys. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela de Veterinaria; ArgentinaFil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Matthijnssens, Jelle. University of Leuven. Department of Microbiology and Immunology; Bélgica. Rega Institute for Medical Research. Laboratory of Viral Metagenomics; Bélgica.Fil: Thiry, Etienne. University of Liège. Faculty of Veterinary Medicine. Fundamental and Applied Research for Animal and Health Centre. Department of Infectious and Parasitic Diseases. Veterinary Virology and Animal Viral Diseases; BélgicaFil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; ArgentinaFil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina2017-09-14T13:59:44Z2017-09-14T13:59:44Z2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfhttp://hdl.handle.net/20.500.12123/1219http://www.sciencedirect.com/science/article/pii/S156713481630065X1567-1348 (Print)1567-7257 (Online)https://doi.org/10.1016/j.meegid.2016.02.034Infection, genetics and evolution 40 : 144-150. (June 2016)reponame:INTA Digital (INTA)instname:Instituto Nacional de Tecnología AgropecuariaengArgentina (nation)info:eu-repo/semantics/restrictedAccess2025-09-29T13:44:10Zoai:localhost:20.500.12123/1219instacron:INTAInstitucionalhttp://repositorio.inta.gob.ar/Organismo científico-tecnológicoNo correspondehttp://repositorio.inta.gob.ar/oai/requesttripaldi.nicolas@inta.gob.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:l2025-09-29 13:44:10.908INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuariafalse
dc.title.none.fl_str_mv Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype
title Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype
spellingShingle Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype
Ferragut, Fátima
Enfermedades de los Animales
Genotipos
Virus
Ganado de Leche
Animal Diseases
Genotypes
Viruses
Dairy Cattle
Norovirus
title_short Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype
title_full Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype
title_fullStr Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype
title_full_unstemmed Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype
title_sort Molecular detection of bovine Noroviruses in Argentinean dairy calves : circulation of a tentative new genotype
dc.creator.none.fl_str_mv Ferragut, Fátima
Vega, Celina Guadalupe
Mauroy, Axel
Conceição-Neto, Nádia
Zeller, Mark
Heylen, Elisabeth
Louge Uriarte, Enrique Leopoldo
Bilbao, Gladys
Bok, Marina
Matthijnssens, Jelle
Thiry, Etienne
Badaracco, Alejandra
Parreño, Gladys Viviana
author Ferragut, Fátima
author_facet Ferragut, Fátima
Vega, Celina Guadalupe
Mauroy, Axel
Conceição-Neto, Nádia
Zeller, Mark
Heylen, Elisabeth
Louge Uriarte, Enrique Leopoldo
Bilbao, Gladys
Bok, Marina
Matthijnssens, Jelle
Thiry, Etienne
Badaracco, Alejandra
Parreño, Gladys Viviana
author_role author
author2 Vega, Celina Guadalupe
Mauroy, Axel
Conceição-Neto, Nádia
Zeller, Mark
Heylen, Elisabeth
Louge Uriarte, Enrique Leopoldo
Bilbao, Gladys
Bok, Marina
Matthijnssens, Jelle
Thiry, Etienne
Badaracco, Alejandra
Parreño, Gladys Viviana
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Enfermedades de los Animales
Genotipos
Virus
Ganado de Leche
Animal Diseases
Genotypes
Viruses
Dairy Cattle
Norovirus
topic Enfermedades de los Animales
Genotipos
Virus
Ganado de Leche
Animal Diseases
Genotypes
Viruses
Dairy Cattle
Norovirus
dc.description.none.fl_txt_mv Bovine noroviruses are enteric pathogens detected in fecal samples of both diarrheic and non-diarrheic calves from several countries worldwide. However, epidemiological information regarding bovine noroviruses is still lacking for many important cattle producing countries from South America. In this study, three bovine norovirus genogroup III sequences were determined by conventional RT-PCR and Sanger sequencing in feces from diarrheic dairy calves from Argentina (B4836, B4848, and B4881, all collected in 2012). Phylogenetic studies based on a partial coding region for the RNA-dependent RNA polymerase (RdRp, 503 nucleotides) of these three samples suggested that two of them (B4836 and B4881) belong to genotype 2 (GIII.2) while the third one (B4848) was more closely related to genotype 1 (GIII.1) strains. By deep sequencing, the capsid region from two of these strains could be determined. This confirmed the circulation of genotype 1 (B4848) together with the presence of another sequence (B4881) sharing its highest genetic relatedness with genotype 1, but sufficiently distant to constitute a new genotype. This latter strain was shown in silico to be a recombinant: phylogenetic divergence was detected between its RNA-dependent RNA polymerase coding sequence (genotype GIII.2) and its capsid protein coding sequence (genotype GIII.1 or a potential norovirus genotype). According to this data, this strain could be the second genotype GIII.2_GIII.1 bovine norovirus recombinant described in literature worldwide. Further analysis suggested that this strain could even be a potential norovirus GIII genotype, tentatively named GIII.4. The data provides important epidemiological and evolutionary information on bovine noroviruses circulating in South America
Inst.de Virología
Fil: Ferragut, Fátima. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Vega, Celina Guadalupe. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Mauroy, Axel. University of Liège. Faculty of Veterinary Medicine. Fundamental and Applied Research for Animal and Health Centre. Department of Infectious and Parasitic Diseases. Veterinary Virology and Animal Viral Diseases; Bélgica
Fil: Conceição-Neto, Nádia. University of Leuven. Department of Microbiology and Immunology; Bélgica. Rega Institute for Medical Research, Laboratory of Viral Metagenomics; Bélgica
Fil: Zeller, Mark. University of Leuven. Department of Microbiology and Immunology. Rega Institute for Medical Research, Laboratory of Viral Metagenomics; Bélgica
Fil: Heylen, Elisabeth. University of Leuven. Department of Microbiology and Immunology. Rega Institute for Medical Research, Laboratory of Viral Metagenomics; Bélgica
Fil: Louge Uriarte, Enrique Leopoldo. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Balcarce. Area de Producción Animal; Argentina
Fil: Bilbao, Gladys. Universidad Nacional del Centro de la Provincia de Buenos Aires. Escuela de Veterinaria; Argentina
Fil: Bok, Marina. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Matthijnssens, Jelle. University of Leuven. Department of Microbiology and Immunology; Bélgica. Rega Institute for Medical Research. Laboratory of Viral Metagenomics; Bélgica.
Fil: Thiry, Etienne. University of Liège. Faculty of Veterinary Medicine. Fundamental and Applied Research for Animal and Health Centre. Department of Infectious and Parasitic Diseases. Veterinary Virology and Animal Viral Diseases; Bélgica
Fil: Badaracco, Alejandra. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
Fil: Parreño, Viviana. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Virología; Argentina
description Bovine noroviruses are enteric pathogens detected in fecal samples of both diarrheic and non-diarrheic calves from several countries worldwide. However, epidemiological information regarding bovine noroviruses is still lacking for many important cattle producing countries from South America. In this study, three bovine norovirus genogroup III sequences were determined by conventional RT-PCR and Sanger sequencing in feces from diarrheic dairy calves from Argentina (B4836, B4848, and B4881, all collected in 2012). Phylogenetic studies based on a partial coding region for the RNA-dependent RNA polymerase (RdRp, 503 nucleotides) of these three samples suggested that two of them (B4836 and B4881) belong to genotype 2 (GIII.2) while the third one (B4848) was more closely related to genotype 1 (GIII.1) strains. By deep sequencing, the capsid region from two of these strains could be determined. This confirmed the circulation of genotype 1 (B4848) together with the presence of another sequence (B4881) sharing its highest genetic relatedness with genotype 1, but sufficiently distant to constitute a new genotype. This latter strain was shown in silico to be a recombinant: phylogenetic divergence was detected between its RNA-dependent RNA polymerase coding sequence (genotype GIII.2) and its capsid protein coding sequence (genotype GIII.1 or a potential norovirus genotype). According to this data, this strain could be the second genotype GIII.2_GIII.1 bovine norovirus recombinant described in literature worldwide. Further analysis suggested that this strain could even be a potential norovirus GIII genotype, tentatively named GIII.4. The data provides important epidemiological and evolutionary information on bovine noroviruses circulating in South America
publishDate 2016
dc.date.none.fl_str_mv 2016
2017-09-14T13:59:44Z
2017-09-14T13:59:44Z
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/20.500.12123/1219
http://www.sciencedirect.com/science/article/pii/S156713481630065X
1567-1348 (Print)
1567-7257 (Online)
https://doi.org/10.1016/j.meegid.2016.02.034
url http://hdl.handle.net/20.500.12123/1219
http://www.sciencedirect.com/science/article/pii/S156713481630065X
https://doi.org/10.1016/j.meegid.2016.02.034
identifier_str_mv 1567-1348 (Print)
1567-7257 (Online)
dc.language.none.fl_str_mv eng
language eng
dc.rights.none.fl_str_mv info:eu-repo/semantics/restrictedAccess
eu_rights_str_mv restrictedAccess
dc.format.none.fl_str_mv application/pdf
dc.coverage.none.fl_str_mv Argentina (nation)
dc.source.none.fl_str_mv Infection, genetics and evolution 40 : 144-150. (June 2016)
reponame:INTA Digital (INTA)
instname:Instituto Nacional de Tecnología Agropecuaria
reponame_str INTA Digital (INTA)
collection INTA Digital (INTA)
instname_str Instituto Nacional de Tecnología Agropecuaria
repository.name.fl_str_mv INTA Digital (INTA) - Instituto Nacional de Tecnología Agropecuaria
repository.mail.fl_str_mv tripaldi.nicolas@inta.gob.ar
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