Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay

Autores
Castells, Matías; Caffarena, Rubén Darío; Casaux, María Laura; Schild, Carlos; Castells, Felipe; Castells, Daniel; Victoria, Matías; Riet Correa, Franklin; Giannitti, Federico; Parreño, Gladys Viviana; Colina, Rodney
Año de publicación
2020
Idioma
inglés
Tipo de recurso
artículo
Estado
versión publicada
Descripción
Uruguay is a leading exporter of bovine meat and dairy products, and cattle production is one of the principal economic backbones in this country. A main clinical problem faced by livestock farmers is neonatal calf diarrhea (NCD); however, causes of NCD have not been extensively studied in Uruguay. Bovine norovirus (BoNoV) has been proposed as one of the possible etiologies of NCD as experimentally infected calves developed diarrhea and enteropathy, although limited information is available from field surveys. The aims of this study were to determine the frequency of infection, to investigate possible risk factors, and to determine the molecular diversity of BoNoV in Uruguay. A total of 761 samples of feces or intestinal contents from dairy and beef calves were analyzed through RT-qPCR. The overall frequency of detection of BoNoV was 66.1% with higher frequency in dairy (70.5%) than beef (15.9%) calves (p < 0.01). BoNoV was detected similarly in diarrheic (78.8%) and non-diarrheic (76.2%) dairy calves (p = 0.50). Calves ≤2 weeks of age (84%) were infected more often than older (62.7%) calves (p < 0.01). Phylogenetic analysis confirmed the presence of GIII.1 and GIII.2 genotypes. In addition, we reported the circulation of recombinant strains and the detection of a strain with the recently described novel VP1 genotype. This study represents the first report describing the circulation, the associated risk factors, and the molecular diversity of BoNoV in Uruguay.
Fil: Castells, Matías. Universidad de la República; Uruguay
Fil: Caffarena, Rubén Darío. Universidad de la República; Uruguay
Fil: Casaux, María Laura. No especifíca;
Fil: Schild, Carlos. No especifíca;
Fil: Castells, Felipe. Universidad de la Republica; Uruguay
Fil: Castells, Daniel. Universidad de la República; Uruguay
Fil: Victoria, Matías. Universidad de la República; Uruguay
Fil: Riet Correa, Franklin. No especifíca;
Fil: Giannitti, Federico. No especifíca;
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; Argentina
Fil: Colina, Rodney. Universidad de la República; Uruguay
Materia
BOVINE NOROVIRUS
CATTLE
DIARRHEA
GENOTYPES
URUGUAY
Nivel de accesibilidad
acceso abierto
Condiciones de uso
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
Repositorio
CONICET Digital (CONICET)
Institución
Consejo Nacional de Investigaciones Científicas y Técnicas
OAI Identificador
oai:ri.conicet.gov.ar:11336/145951
Acceder
id CONICETDig_78ea0c8d96594303bf5e028c29069fa9
oai_identifier_str oai:ri.conicet.gov.ar:11336/145951
network_acronym_str CONICETDig
repository_id_str 3498
network_name_str CONICET Digital (CONICET)
spelling Detection, risk factors and molecular diversity of norovirus GIII in cattle in UruguayCastells, MatíasCaffarena, Rubén DaríoCasaux, María LauraSchild, CarlosCastells, FelipeCastells, DanielVictoria, MatíasRiet Correa, FranklinGiannitti, FedericoParreño, Gladys VivianaColina, RodneyBOVINE NOROVIRUSCATTLEDIARRHEAGENOTYPESURUGUAYhttps://purl.org/becyt/ford/4.3https://purl.org/becyt/ford/4Uruguay is a leading exporter of bovine meat and dairy products, and cattle production is one of the principal economic backbones in this country. A main clinical problem faced by livestock farmers is neonatal calf diarrhea (NCD); however, causes of NCD have not been extensively studied in Uruguay. Bovine norovirus (BoNoV) has been proposed as one of the possible etiologies of NCD as experimentally infected calves developed diarrhea and enteropathy, although limited information is available from field surveys. The aims of this study were to determine the frequency of infection, to investigate possible risk factors, and to determine the molecular diversity of BoNoV in Uruguay. A total of 761 samples of feces or intestinal contents from dairy and beef calves were analyzed through RT-qPCR. The overall frequency of detection of BoNoV was 66.1% with higher frequency in dairy (70.5%) than beef (15.9%) calves (p < 0.01). BoNoV was detected similarly in diarrheic (78.8%) and non-diarrheic (76.2%) dairy calves (p = 0.50). Calves ≤2 weeks of age (84%) were infected more often than older (62.7%) calves (p < 0.01). Phylogenetic analysis confirmed the presence of GIII.1 and GIII.2 genotypes. In addition, we reported the circulation of recombinant strains and the detection of a strain with the recently described novel VP1 genotype. This study represents the first report describing the circulation, the associated risk factors, and the molecular diversity of BoNoV in Uruguay.Fil: Castells, Matías. Universidad de la República; UruguayFil: Caffarena, Rubén Darío. Universidad de la República; UruguayFil: Casaux, María Laura. No especifíca;Fil: Schild, Carlos. No especifíca;Fil: Castells, Felipe. Universidad de la Republica; UruguayFil: Castells, Daniel. Universidad de la República; UruguayFil: Victoria, Matías. Universidad de la República; UruguayFil: Riet Correa, Franklin. No especifíca;Fil: Giannitti, Federico. No especifíca;Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; ArgentinaFil: Colina, Rodney. Universidad de la República; UruguayElsevier Science2020-12info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://purl.org/coar/resource_type/c_6501info:ar-repo/semantics/articuloapplication/pdfapplication/pdfhttp://hdl.handle.net/11336/145951Castells, Matías; Caffarena, Rubén Darío; Casaux, María Laura; Schild, Carlos; Castells, Felipe; et al.; Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay; Elsevier Science; Infection, Genetics and Evolution; 86; 12-2020; 1-71567-1348CONICET DigitalCONICETenginfo:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2020.104613info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1567134820304445info:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-sa/2.5/ar/reponame:CONICET Digital (CONICET)instname:Consejo Nacional de Investigaciones Científicas y Técnicas2025-09-29T10:26:15Zoai:ri.conicet.gov.ar:11336/145951instacron:CONICETInstitucionalhttp://ri.conicet.gov.ar/Organismo científico-tecnológicoNo correspondehttp://ri.conicet.gov.ar/oai/requestdasensio@conicet.gov.ar; lcarlino@conicet.gov.arArgentinaNo correspondeNo correspondeNo correspondeopendoar:34982025-09-29 10:26:15.884CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicasfalse
dc.title.none.fl_str_mv Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
title Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
spellingShingle Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
Castells, Matías
BOVINE NOROVIRUS
CATTLE
DIARRHEA
GENOTYPES
URUGUAY
title_short Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
title_full Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
title_fullStr Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
title_full_unstemmed Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
title_sort Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay
dc.creator.none.fl_str_mv Castells, Matías
Caffarena, Rubén Darío
Casaux, María Laura
Schild, Carlos
Castells, Felipe
Castells, Daniel
Victoria, Matías
Riet Correa, Franklin
Giannitti, Federico
Parreño, Gladys Viviana
Colina, Rodney
author Castells, Matías
author_facet Castells, Matías
Caffarena, Rubén Darío
Casaux, María Laura
Schild, Carlos
Castells, Felipe
Castells, Daniel
Victoria, Matías
Riet Correa, Franklin
Giannitti, Federico
Parreño, Gladys Viviana
Colina, Rodney
author_role author
author2 Caffarena, Rubén Darío
Casaux, María Laura
Schild, Carlos
Castells, Felipe
Castells, Daniel
Victoria, Matías
Riet Correa, Franklin
Giannitti, Federico
Parreño, Gladys Viviana
Colina, Rodney
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv BOVINE NOROVIRUS
CATTLE
DIARRHEA
GENOTYPES
URUGUAY
topic BOVINE NOROVIRUS
CATTLE
DIARRHEA
GENOTYPES
URUGUAY
purl_subject.fl_str_mv https://purl.org/becyt/ford/4.3
https://purl.org/becyt/ford/4
dc.description.none.fl_txt_mv Uruguay is a leading exporter of bovine meat and dairy products, and cattle production is one of the principal economic backbones in this country. A main clinical problem faced by livestock farmers is neonatal calf diarrhea (NCD); however, causes of NCD have not been extensively studied in Uruguay. Bovine norovirus (BoNoV) has been proposed as one of the possible etiologies of NCD as experimentally infected calves developed diarrhea and enteropathy, although limited information is available from field surveys. The aims of this study were to determine the frequency of infection, to investigate possible risk factors, and to determine the molecular diversity of BoNoV in Uruguay. A total of 761 samples of feces or intestinal contents from dairy and beef calves were analyzed through RT-qPCR. The overall frequency of detection of BoNoV was 66.1% with higher frequency in dairy (70.5%) than beef (15.9%) calves (p < 0.01). BoNoV was detected similarly in diarrheic (78.8%) and non-diarrheic (76.2%) dairy calves (p = 0.50). Calves ≤2 weeks of age (84%) were infected more often than older (62.7%) calves (p < 0.01). Phylogenetic analysis confirmed the presence of GIII.1 and GIII.2 genotypes. In addition, we reported the circulation of recombinant strains and the detection of a strain with the recently described novel VP1 genotype. This study represents the first report describing the circulation, the associated risk factors, and the molecular diversity of BoNoV in Uruguay.
Fil: Castells, Matías. Universidad de la República; Uruguay
Fil: Caffarena, Rubén Darío. Universidad de la República; Uruguay
Fil: Casaux, María Laura. No especifíca;
Fil: Schild, Carlos. No especifíca;
Fil: Castells, Felipe. Universidad de la Republica; Uruguay
Fil: Castells, Daniel. Universidad de la República; Uruguay
Fil: Victoria, Matías. Universidad de la República; Uruguay
Fil: Riet Correa, Franklin. No especifíca;
Fil: Giannitti, Federico. No especifíca;
Fil: Parreño, Gladys Viviana. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación En Ciencias Veterinarias y Agronómicas. Instituto de Virología E Innovaciones Tecnológicas. - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Pque. Centenario. Instituto de Virología E Innovaciones Tecnológicas; Argentina
Fil: Colina, Rodney. Universidad de la República; Uruguay
description Uruguay is a leading exporter of bovine meat and dairy products, and cattle production is one of the principal economic backbones in this country. A main clinical problem faced by livestock farmers is neonatal calf diarrhea (NCD); however, causes of NCD have not been extensively studied in Uruguay. Bovine norovirus (BoNoV) has been proposed as one of the possible etiologies of NCD as experimentally infected calves developed diarrhea and enteropathy, although limited information is available from field surveys. The aims of this study were to determine the frequency of infection, to investigate possible risk factors, and to determine the molecular diversity of BoNoV in Uruguay. A total of 761 samples of feces or intestinal contents from dairy and beef calves were analyzed through RT-qPCR. The overall frequency of detection of BoNoV was 66.1% with higher frequency in dairy (70.5%) than beef (15.9%) calves (p < 0.01). BoNoV was detected similarly in diarrheic (78.8%) and non-diarrheic (76.2%) dairy calves (p = 0.50). Calves ≤2 weeks of age (84%) were infected more often than older (62.7%) calves (p < 0.01). Phylogenetic analysis confirmed the presence of GIII.1 and GIII.2 genotypes. In addition, we reported the circulation of recombinant strains and the detection of a strain with the recently described novel VP1 genotype. This study represents the first report describing the circulation, the associated risk factors, and the molecular diversity of BoNoV in Uruguay.
publishDate 2020
dc.date.none.fl_str_mv 2020-12
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
http://purl.org/coar/resource_type/c_6501
info:ar-repo/semantics/articulo
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/11336/145951
Castells, Matías; Caffarena, Rubén Darío; Casaux, María Laura; Schild, Carlos; Castells, Felipe; et al.; Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay; Elsevier Science; Infection, Genetics and Evolution; 86; 12-2020; 1-7
1567-1348
CONICET Digital
CONICET
url http://hdl.handle.net/11336/145951
identifier_str_mv Castells, Matías; Caffarena, Rubén Darío; Casaux, María Laura; Schild, Carlos; Castells, Felipe; et al.; Detection, risk factors and molecular diversity of norovirus GIII in cattle in Uruguay; Elsevier Science; Infection, Genetics and Evolution; 86; 12-2020; 1-7
1567-1348
CONICET Digital
CONICET
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1016/j.meegid.2020.104613
info:eu-repo/semantics/altIdentifier/url/https://www.sciencedirect.com/science/article/pii/S1567134820304445
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
eu_rights_str_mv openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/2.5/ar/
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Elsevier Science
publisher.none.fl_str_mv Elsevier Science
dc.source.none.fl_str_mv reponame:CONICET Digital (CONICET)
instname:Consejo Nacional de Investigaciones Científicas y Técnicas
reponame_str CONICET Digital (CONICET)
collection CONICET Digital (CONICET)
instname_str Consejo Nacional de Investigaciones Científicas y Técnicas
repository.name.fl_str_mv CONICET Digital (CONICET) - Consejo Nacional de Investigaciones Científicas y Técnicas
repository.mail.fl_str_mv dasensio@conicet.gov.ar; lcarlino@conicet.gov.ar
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score 13.070432

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